CN107667976B - Method for producing seeds of male-female self-discriminating high-yield Luhua feather green-shell laying hens - Google Patents
Method for producing seeds of male-female self-discriminating high-yield Luhua feather green-shell laying hens Download PDFInfo
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- 210000003746 feather Anatomy 0.000 title claims abstract description 77
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 25
- 241000287828 Gallus gallus Species 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims abstract description 27
- 230000001488 breeding effect Effects 0.000 claims abstract description 27
- 230000008774 maternal effect Effects 0.000 claims abstract description 25
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 22
- 230000008775 paternal effect Effects 0.000 claims abstract description 18
- 210000003278 egg shell Anatomy 0.000 claims abstract description 11
- 235000013601 eggs Nutrition 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 14
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 238000009396 hybridization Methods 0.000 claims description 7
- 230000009027 insemination Effects 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 4
- 239000003147 molecular marker Substances 0.000 claims description 3
- 238000009402 cross-breeding Methods 0.000 claims 1
- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 244000144977 poultry Species 0.000 abstract description 2
- 230000000644 propagated effect Effects 0.000 abstract 1
- 235000013330 chicken meat Nutrition 0.000 description 23
- 230000002068 genetic effect Effects 0.000 description 5
- 230000036750 egg laying performance Effects 0.000 description 3
- 210000003765 sex chromosome Anatomy 0.000 description 3
- 108700003861 Dominant Genes Proteins 0.000 description 2
- 210000000436 anus Anatomy 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000037237 body shape Effects 0.000 description 2
- 239000013065 commercial product Substances 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
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- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 101150023929 egg gene Proteins 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
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Abstract
The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a method for producing male and female self-distinguished high-yield Luhua feather green-shell laying hens. The method for producing the seeds of the self-sexing high-yield Luhua feather green-shell laying hens comprises the steps of forming a first paternal line A and a first maternal line B by utilizing a non-Luhua feather high-yield laying hen A line and a non-Luhua feather high-yield laying hen B line; after the first paternal line A and the first maternal line B are hybridized and purely propagated, high-yield slow-feather non-Luhua-feather non-green-shell hens are left to be selected as a second maternal line AB; selecting fast-feather and non-green-shell cocks from the Luhua chicken strains as male parents, selecting fast-feather and green-shell hens from the non-Luhua chicken strains as female parents, hybridizing the two families, performing pure breeding, and selecting genes of fast-feather, Luhua-feather and green-shell to be homozygous to serve as a terminal father line C; and hybridizing the terminal paternal line C and the second maternal line AB to obtain a commercial generation, wherein slow feather is cock and fast feather is hen. The high-yield Luhua feather green-shell laying hen capable of self-distinguishing male and female obtained by the seed production method has the advantages of high yield, self-distinguishing male and female and green shell egg shells.
Description
Technical Field
The invention belongs to the technical field of poultry genetic breeding, and particularly relates to a method for producing male and female self-distinguished high-yield Luhua feather green-shell laying hens.
Background
The method has the advantages that the breadth of China is wide, the local variety resources are rich, the variety with local characteristics is formed in different regions, and the characteristic characters are outstanding. Wenshang Luhua chicken has the advantages of beautiful appearance, delicious meat quality and rich nutrition of eggs, but the yield is low. The green shell eggs are bright in color and are deeply loved by consumers, so that the green shell eggs have higher market price but also have the defect of low egg yield. The quality character of the green shell egg is dominant gene control on an autosome, so that important significance is achieved if the green shell egg gene can be introduced into Wenshang Luhua chickens, a new green shell strain with the body and appearance characteristics of the Wenshang Luhua chickens is cultivated, and then the new green shell strain is hybridized with a high-yield variety, so that the egg laying performance of commercial green shell laying hens is improved. A self-distinguishing male-female system is established by utilizing the sex-linked genetic principle of fast and slow feathers, the polymerization of the Luhua feather character, the green shell egg character, the high yield character and the fast and slow feathers is realized, and a new variety which has the characteristics of keeping the body shape and appearance of the Wenshang Luhua chicken and can produce the green shell egg and greatly improve the egg laying performance is cultivated, so that the method has wide market prospect.
Disclosure of Invention
In order to make up for the defects of the prior art, the invention provides the seed production method of the self-sexing high-yield Luhua feather green-shell laying hens, which is scientific and reasonable in seed production and simple in operation.
The invention is realized by the following technical scheme: a seed production method of a self-sexing high-yield Luhua feather green-shell laying hen comprises the following operation steps:
s1: mooring and selecting slow-feather and non-green-shell individuals by using non-Luhua-feather high-yield laying hens A to form a first paternal line A; selecting slow feather and non-green shell individuals by using a non-Luhua feather high-yield laying hen B line to form a first maternal line B;
s2: hybridizing a first paternal line A with a first maternal line B to obtain F1 pure breeding, and selecting high-yield, slow-feather, non-Luhua-feather and non-green-shell hens as a second maternal line AB;
s3: selecting fast-feathering and non-green-shell cocks from Luhua chicken strains as male parents, selecting fast-feathering and green-shell hens from the non-Luhua chicken strains as female parents, hybridizing the two, and then carrying out pure propagation, wherein the selected homozygous genes are fast-feathering, Luhua-feathering and green-shell genes, and the strain capable of being stably inherited is a terminal father line C;
s4: and hybridizing the terminal male line C with the second female line AB to obtain a commercial generation which can self identify male and female and has the characteristics of high yield, Luhua feather and green shell, wherein the slow feather is a cock, and the fast feather is a hen.
In step S3, the fast feather gene, the green hull gene and the Luhua feather gene of the male parent are homozygous genotype ZkZkOO and ZBZB。
In step S3, Luhua feather and fast feather are selected from F1 'generation obtained by hybridization of male parent and female parent, meanwhile, the hen produces green shell egg, and the obtained F2' generation is selected and reserved according to the same selection standard to continue to be purely bred for many times, and simultaneously, molecular marker-assisted selection and test cross experiments are carried out, and finally, the fast feather, Luhua feather and green shell strains of homozygous genotypes are bred as a terminal father line C.
In step S4, the eggshell color trait genotype of the terminal paternal line C is a dominant homozygous genotype OO, which is crossed with OO of a non-green eggshell genotype, and the obtained offspring genotype is OO, and the eggshell color phenotype is green.
In step S4, the Luhua feather character gene of terminal paternal line C is dominant homozygous genotype ZBZBIt is combined with genotype ZbAfter the second maternal line AB of the W, which is not provided with Luhua feathers, is hybridized, the feathers of the commercial cock and the hen are both presented with Luhua feathers.
The invention has the following beneficial effects: the preparation method is simple, adopts a three-line matched seed production method, takes a high-yield slow-feather line as a first paternal line A, takes a high-yield slow-feather line as a first maternal line B, hybridizes the first paternal line A with the first maternal line B to obtain a second maternal line AB with high yield and slow feather, takes homozygous fast-feather, luhua and green-shell lines as a terminal paternal line C, and assembles the second maternal line AB and the terminal paternal line C to produce a matched line, and has the following technical advantages:
1. the second maternal AB has high egg yield, proper egg weight and high feed utilization efficiency, and each parental generation breeding hen can produce 110 commercial generation hens at 72 weeks old, so that the seed production cost is reduced;
2. the egg yield of the Wenshang Luhua chicken of 72 weeks is generally within 180, the egg yield of the commercial product of the invention is improved by about 45 percent compared with that of the pure Wenshang Luhua chicken, and the egg yield of the commercial product of 72 weeks is up to more than 260;
3. the eggshell color of the commercial chicken substitutes is green, so that the consumption requirement of consumers on the eggshell color is met;
4. the commercial cock and hen produced by the method keep the body shape and appearance characteristics of the Wenshang Luhua chicken, and are popular with consumers;
5. the commodity generation produced finally can identify male and female according to feather speed, so that cross contamination possibly caused by anus turning identification is avoided, and the identification cost is saved.
The method meets the consumption requirements of consumers on the special local breeding hens, the value of the produced commercial cock and hen is obviously improved compared with that of the common laying hens, the innovative utilization of excellent variety resources in China is realized, and the economic and social benefits are good.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a pattern diagram of a mating line for producing male and female self-discriminating high-yield Luhua feather green-shell laying hens of the present invention;
FIG. 2 is a genetic map of breeding lines of a self-sexing high-yield Luhua feather green-shell laying hen according to an embodiment of the present invention;
FIG. 3 is a breeding flow chart of the green hull and Luhua feather homozygous terminal father line C of the invention;
wherein:
Z/W: it is expressed as sex chromosome of chicken, ZZ is male, ZW is female;
K/K: the genes representing slow-feather and fast-feather traits are positioned on a sex chromosome Z and are sex-linked genetic genes;
B/B: the genes representing Luhua feather and non-Luhua feather are positioned on a sex chromosome Z and are sex-linked genetic genes;
O/O: indicating that the green shell and non-green shell genes are located on the autosome and are autosomal dominant genes.
Detailed Description
In order to clearly illustrate the technical features of the present invention, the following detailed description of the present invention with reference to the accompanying drawings is provided, but the present invention is not limited thereto.
Examples
As shown in fig. 1-3, the method for producing the self-sexing high-yield Luhua feather green-shell laying hen comprises the following operation steps:
s1: high-yield laying hen Jinghong No. 1 laying hen A line cock and hen are used as the former generation, slow feather individuals are selected for breeding the first father line A at the age of 1 day after hatching, heterozygous genes are eliminated by means of transverse cross assay and the like, and the genotype is selected to be the male ZKbZKboo，♀ZKbWoo is subjected to hybridization breeding, weak chicks are eliminated, individual cages are adopted for feeding, artificial insemination is performed, the number of seeds reserved in each generation is more than 500, and the male-female ratio is 1: 10; finally, the homozygous genotype cock Z is left to be selectedKbZKboo, forming a first paternal system A;
high-yield laying hen Jinghong No. 1 laying hen B line cock and hen are used as the former generation, slow feather individuals are selected for breeding of a first maternal line B at the age of 1 day after hatching, heterozygous genes are eliminated by means of transverse cross assay and the like, and the selected genotype is the male ZKbZKboo and male parent ZKbWoo is subjected to hybridization breeding, weak chicks are eliminated, individual cages are adopted for feeding, artificial insemination is performed, the number of seeds reserved in each generation is more than 2000, and the male-female ratio is 1: 10; finally, the homozygous genotype hen Z is left to be selectedKbWoo, forming a first parent system B.
S2: crossing the first paternal line A and the first maternal line B to obtain F1 to obtain a second maternal line AB with homozygous genotype ZKbWoo；
In the seed production process, a first paternal line A and a first maternal line B are respectively bred and cultured by utilizing a high-yield layer Jinghong No. 1 layer A line and a high-yield layer Jinghong No. 1 layer B line, and then the first paternal line A with high yield and stable characters is hybridized with the first maternal line B to obtain a second maternal line AB with slow feather and stable genetic characteristics, which plays an important role in subsequently improving the egg yield of commercial generations.
S3: selecting fast-feathering and non-green-shell cock from Wenshang Luhua chicken as male parent, wherein the genotype is Henan ZkBZkBoo, selecting fast-feather green-shell hen from Dongxiang green-shell laying hens asIs female parent and its genotype is male parent ZkbWOO, F1' generation obtained by the hybridization of the two generations are fast feather, reed flower feather and green shell, and the genotype of the cock is equal to ZkBZkbOo, female parent of hen genotype ZkBWOo, respectively; then pure breeding is carried out, individuals with the characters of fast feather, Luhua feather and green shell are reserved in the obtained F2' generation, and the genotype of the obtained cock may be ZkBZkBOo、ZkBZkBOO、ZkBZkbOo、ZkBZkbOO, hen genotype may be ZkBWOo、ZkBWOO, and selecting homozygous genotype Z by a molecular marker assisted selection or test cross methodkBZkBOO、♀ZkBPerforming pure breeding again on the WOO individuals, eliminating weak chicks, keeping healthy chicks, adopting single-cage breeding and artificial insemination, wherein the seed number of each generation is more than 500, and the male-female ratio is 1: 10; finally, the homozygous cock is left and selected as a terminal father line C, and the genotype is as the sex of the cockkBZkBOO。
S4: c and Z of father system of the terminalkBZkBOO and AB/Z of the second maternal lineKbThe commercial generation is obtained by crossing Woo to obtain F2, the phenotype of which is high yield, Luhua feather and green shell, wherein the cock is slow feather, and the genotype is male ZkBZKbOo, hen is fast feather, its genotype is male parent ZkBWOo, the chicken can be quickly identified by fast and slow feather.
In the above method, the second maternal line AB can be obtained by crossing Hailan A line and Hailan B line, and the breeding and raising method is the same as that described in step S1.
The commercial generation of the invention can be in accordance with the cage-rearing mode of modern laying hen production, and has the advantages of high rearing efficiency, convenient management and contribution to ensuring the product quality safety.
Examples of the experiments
1. The production performance of parental breeding hens is compared and shown in table 1:
experimental groups: self-sexing parental breeding hens of the high-yield Luhua feather green shell laying hens; control group: a pure parent breeding chicken of luhua wenshang.
TABLE 1 Productivity of parental breeding hens bred in accordance with the present invention
As can be seen from Table 1, the production performance of the parent breeding hens is higher, the number of eggs laid by the breeding hens is improved by more than 120 compared with that of the pure Wenshang Luhua chicken used as a female parent and is improved by more than 60% compared with that of the Wenshang Luhua chicken in 72 weeks, and the breeding cost is obviously reduced; the parent-generation hens are the father-line hens in the seed production process of the high-yield laying hens, the feather color is recessive golden feather, so that the feather color of the offspring of the parents-generation hens after the parents-generation hens are hybridized with the reed-blossom-feather cocks is represented as the reed-blossom-feather, the production performance of the parents-generation hens is equivalent to that of the high-yield laying hens (the high-yield laying hens lay 300 eggs at the age of 72 weeks), and the parent-generation hens of the seed production method have higher egg laying performance.
2. The comprehensive production performance of the commercial chicken substitutes is compared and shown in the table 2:
experimental groups: the method comprises the following steps of (1) self-sexing high-yield commercial generation cock (commercial generation cock for short), self-sexing high-yield commercial generation hen (commercial generation hen for short); control group: the commercial chicken of Wenshang Luhua chicken was bred as cock, and the commercial chicken of Wenshang Luhua chicken was bred as hen.
TABLE 2 comparison of the traits of commercial chicken substitutes
And (4) analyzing results: as can be seen from the table 2, the eggshells of the commercial eggs bred by the method 1 are green, so that the consumption requirements of consumers on the eggshell color are met; 2. the commercial generation 72-week-old egg yield of the invention is up to 260, which is improved by over 45 percent compared with the pure Wenshang Luhua chicken, thus enhancing the market competitiveness; 3. the commercial cock generations and the commercial hen generations are slow feather generations and fast feather generations, and male and female generations can be distinguished according to the feather speed, so that cross contamination possibly caused by anus turning identification is avoided, and the identification cost is saved.
Claims (5)
1. A seed production method of a male-female self-distinguished high-yield Luhua feather green-shell laying hen is characterized by comprising the following operation steps:
s1: high-yield laying hen Jinghong No. 1 laying hen A line cock and hen are used as a former generation, a slow feather individual is left and selected for breeding a first father line at the age of 1 day after hatching, heterozygous genes are eliminated by a cross-breeding determination method, and the genotype of the first father line Z is selectedKbZKboo，♀ZKbWoo is subjected to hybridization breeding, weak chicks are eliminated, individual cages are adopted for feeding, artificial insemination is performed, the number of seeds reserved in each generation is more than 500, and the male-female ratio is 1: 10; finally, the homozygous genotype cock Z is left to be selectedKbZKboo, forming a first paternal system;
high-yield laying hen Jinghong No. 1 laying hen B line cock and hen are used as a first ancestor, slow feather individuals are selected for breeding of a first maternal line at the age of 1 day after hatching, heterozygous genes are eliminated by a cross-over determination means, and the genotype is selected as a male ZKbZKboo and male parent ZKbWoo is subjected to hybridization breeding, weak chicks are eliminated, individual cages are adopted for feeding, artificial insemination is performed, the number of seeds reserved in each generation is more than 2000, and the male-female ratio is 1: 10; finally, the homozygous genotype hen Z is left to be selectedKbWoo, forming a first maternal line;
s2: hybridizing a first paternal line and a first maternal line to obtain F1 pure breeding, and selecting high-yield, slow-feather, non-Luhua-feather and non-green-shell hens as a second maternal line;
s3: selecting fast-feather and non-green-shell cocks from Luhua chicken strains as male parents, selecting fast-feather and green-shell hens from the non-Luhua chicken strains as female parents, hybridizing the two, and then carrying out pure breeding, wherein the selected homozygous genes are fast-feather, Luhua-feather and green-shell genes, and the strain capable of being stably inherited is a terminal father line;
s4: and hybridizing the terminal male line and the second female line to obtain a commercial generation which can self identify male and female and has the characteristics of high yield, Luhua feather and green shell, wherein the slow feather is a cock, and the fast feather is a hen.
2. The method for producing eggs of Luhua feather laying hens capable of producing high yield eggs by self-sexing according to claim 1, wherein the method comprises the following steps: in step S3, the fast feather gene, the green hull gene and the Luhua feather gene of the terminal father line are homozygous genotypes ZkZkOO and ZBZB。
3. The method for producing eggs of Luhua feather laying hens capable of producing high yield eggs by self-sexing as claimed in claim 2, wherein the method comprises the following steps: in step S3, Luhua feather and fast feather are selected from F1 'generation obtained by hybridization of male parent and female parent, meanwhile, the hen produces green shell egg, and the obtained F2' generation is selected and reserved according to the same selection standard to continue to be purely bred for many times, and simultaneously, molecular marker-assisted selection and test cross experiments are carried out, and finally, the fast feather, Luhua feather and green shell strains of homozygous genotypes are bred as terminal father lines.
4. The method for producing eggs of Luhua feather laying hens capable of producing high yield eggs by self-sexing according to claim 1, wherein the method comprises the following steps: in step S4, the eggshell color trait genotype of the terminal paternal line is dominant homozygous genotype OO, which is crossed with OO of a non-green eggshell genotype to obtain an offspring genotype of OO, which is green in all eggshell color phenotypes.
5. The method for producing eggs of Luhua feather laying hens capable of producing high yield eggs by self-sexing according to claim 1, wherein the method comprises the following steps: in step S4, the Luhua feather character gene of the terminal father line is dominant homozygous genotype ZBZBIt is combined with genotype ZbAfter the second female line of the W, which is not provided with the Luhua feather, is hybridized, the feather color of the commercial cock and the feather color of the hen are both presented as the Luhua feather color.
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| CN108496896A (en) * | 2018-04-09 | 2018-09-07 | 徐杰 | A kind of green shell plumage color of high yield is from other laying hen breeding method and its application |
| CN109315350A (en) * | 2018-07-12 | 2019-02-12 | 湖北欣华生态畜禽开发有限公司 | A kind of breeding method of the green shell high-yield egg chicken of recessive white feather short-foot |
| CN109122579B (en) * | 2018-09-30 | 2021-11-05 | 辽宁农业职业技术学院 | Method for improving self-identification sexing accuracy of fast and slow feathers of big-bone chicken |
| CN111345267B (en) * | 2020-04-08 | 2021-11-19 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN114667965A (en) * | 2022-02-17 | 2022-06-28 | 江苏省家禽科学研究所科技创新有限公司 | Method for breeding high-reproduction, high-efficiency and high-quality dual-purpose black-feather chickens |
| CN115226671A (en) * | 2022-08-05 | 2022-10-25 | 长顺天农绿壳蛋鸡实业有限公司 | Method for producing hybrid seeds of self-sexing Changshun green-shell laying hens matched line |
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