CN103975898B - The breeding method of layer of green-shell egg pure lines - Google Patents

The breeding method of layer of green-shell egg pure lines Download PDF

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CN103975898B
CN103975898B CN201410205725.4A CN201410205725A CN103975898B CN 103975898 B CN103975898 B CN 103975898B CN 201410205725 A CN201410205725 A CN 201410205725A CN 103975898 B CN103975898 B CN 103975898B
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green
egg
shell
genotype
cock
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CN103975898A (en
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蒋小松
杜华锐
杨朝武
李晴云
余春林
邱莫寒
张增荣
李雯
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Sichuan Animal Science Academy
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Sichuan Animal Science Academy
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Abstract

The present invention relates to a kind of animal breeding method, particularly the breeding method that is sheerly of a kind of layer of green-shell egg, the black chicken of Yi Jiuyuan is material, and can promote the variety purification for other with green shell blood relationship chicken; It comprises the steps: the selection of the first step, parent; The production of second step, the oocyan gene individuality that isozygotys; 3rd step, colony expand numerous; The present invention realizes layer of green-shell egg and to isozygoty the structure of colony, by the heterozygous genotypes under screening natural conditions, by the hybridization scheme of science, produce homozygous genotype, and reach the numerous object of expansion fast, the variety purification for other with green shell blood relationship chicken can be promoted.

Description

The breeding method of layer of green-shell egg pure lines
Technical field
The present invention relates to a kind of animal breeding method, particularly the breeding method that is sheerly of a kind of layer of green-shell egg, the black chicken of Yi Jiuyuan is material, and can promote the variety purification for other with green shell blood relationship chicken.
Background technology
Improve the important directions having become laying hen breeding research with raising egg quality.The egg with green shell mark contains a large amount of lecithin, vitamin, amino acid, trace element etc., is subject to consumers in general and likes.The commercial generation layer of green-shell egg of hybridizing in the market, although its egg produced is based on green shell, green shell ratio is from 60 ~ 90% not etc., low in economic efficiency.Have the chicken of 5% to produce green-egg-shelled in the black chicken of old institute, and under nature, the genotype of oocyan gene mostly is heterozygote, for the utilization of the green shell proterties of this kind brings difficulty.The green shell proterties of chicken is qualitative character, is controlled, be positioned on number one the short arm of a chromosome, in complete dominance by oocyan gene Oocyan (O).For a hen, eggshell color and the depth thereof are relatively fixed, and have inheritance, and the root that green shell rate is low, be layer of green-shell egg provenance impure caused by, therefore, the kind provenance with green shell blood relationship is purified, for the development and utilization of this kind, and the seed selection of layer of green-shell egg kind, significant.
Summary of the invention
Technical problem to be solved by this invention is to provide the breeding method of a kind of layer of green-shell egg pure lines, by the purification to cock and hen oocyan gene, builds layer of green-shell egg colony of isozygotying.
For achieving the above object, the technical solution used in the present invention is: a kind of breeding method of layer of green-shell egg pure lines, comprises the steps:
The selection of the first step, parent
(1) female parent selection, the hen producing green-egg-shelled in the black chicken of Yi Jiuyuan, for maternal, determines its genotype OO and Oo by observing its eggshell color of laying eggs; Wherein oocyan gene heterozygote individual genotype is Oo, produce in egg the white shell egg that adulterates; Green shell homozygotic individual genotype is OO, and institute lays eggs and is green-egg-shelled;
(2) male parent is selected, adopt the method for families selecting, with the black cock chicken of old institute for male parent, its genotype is OO, Oo and oo, be that oo is hybridized with high yield white shell egg chicken, hen genotype, if institute lays eggs and is white shell in family, then this family cock is oo homozygote, do not carry oocyan gene O, eliminated; If institute's white shell of having laid eggs and green shell in family, then this family cock carries oocyan gene, and genotype is Oo, continues to employ; If lay eggs and be green shell, then this family cock carries oocyan gene, genotype is OO, continues to employ;
The production of second step, the oocyan gene individuality that isozygotys
Female parent selected by the first step and male parent are carried out mating, there are white shell egg and green-egg-shelled in institute in laying eggs, green-egg-shelled is carried out hatching and obtains F1 generation, the genotype of method to the individuality of F1 described in the first step is adopted to differentiate, filter out the cock hen individuality carrying the oocyan gene that isozygotys that hybridization is produced, white shell egg is then eliminated;
3rd step, colony expand numerous
By second step to produce and the cock with the oocyan gene OO that isozygotys identified and hen hybridize, its Offspring F2 is homozygote, realizes fast-propagation colony.
Advantageous Effects of the present invention is: the present invention realizes layer of green-shell egg and to isozygoty the structure of colony, by the heterozygous genotypes under screening natural conditions, by the hybridization scheme of science, produce homozygous genotype, and reach the numerous object of expansion fast, the variety purification for other with green shell blood relationship can be promoted.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The green shell proterties of layer of green-shell egg is qualitative character, is controlled by single-gene Oocyan (O).There is the chicken of 5% to produce green-egg-shelled in the black chicken of old institute, need first screen breeding material.Hen is selected by observing the eggshell color of laying eggs, and the genotype of its oocyan gene is OO or Oo; And whether cock carries oocyan gene, need to be measured by families selecting method.Again through the numerous scheme of a series of expansion, build isozygoty cock and hen colony.
Embodiment
The selection of the first step, parent
(1) maternal selection: the hen producing green-egg-shelled in the black chicken of Yi Jiuyuan is material, its genotype (OO and Oo) is determined by observing its eggshell color of laying eggs, wherein oocyan gene heterozygote individual (Oo) produce in egg the white shell egg that adulterates, homozygotic individual (OO) is laid eggs and is green-egg-shelled;
(2) selection of male parent: the method adopting families selecting, with the black cock chicken of old institute for material, its genotype is OO, Oo and oo, and every cock and 10 high yield white shell egg chicken hens (genotype is oo) are hybridized, and records each family and to lay eggs situation;
If 1. in family, hen is laid eggs and is white shell egg, then the cock genotype of this family is oo, does not namely possess oocyan gene, and this family is eliminated;
If 2. in family, hen has been laid eggs white shell and green shell, then this family cock has had oocyan gene, and genotype is Oo;
If 3. lay eggs and be green shell, then the cock hybridized with it is OO homozygote;
The second, to isozygoty the production of oocyan gene individuality
1. the female parent identifying out by the first step and male parent carry out mating, its egg that produces has white shell egg (eliminating) and green-egg-shelled, green-egg-shelled is carried out hatching and obtains F1 generation, adopt the genotype of method to the individuality of F1 described in the first step to differentiate, filter out the cock hen individuality carrying the oocyan gene that isozygotys that hybridization is produced;
Offspring F1 is the individuality containing oocyan gene (O), and wherein the oocyan gene type of hen is differentiated according to the method for the first step (1); The oocyan gene type of cock is differentiated according to the method for the first step (2);
2., under nature, namely the first step (1), (2) individuality carrying the oocyan gene that isozygotys that can filter out is very few, if exist, can be directly used in expand numerous;
Three, colony expands numerous
By second step to produce and the cock with the oocyan gene that isozygotys identified and hen (OO) hybridize, its Offspring F2 is homozygote, realizes fast-propagation colony;
It should be noted that, the probability of what this step method can be produced isozygoty oocyan gene type individuality is 25%, therefore needs repeatedly to expand numerous, can build the cock and hen colony of carrying the oocyan gene that isozygotys, for the exploitation of layer of green-shell egg.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (1)

1. a breeding method for layer of green-shell egg pure lines, is characterized in that, comprise the steps:
The selection of the first step, parent
(1) female parent selection, the hen producing green-egg-shelled in the black chicken of Yi Jiuyuan, for maternal, determines its genotype OO and Oo by observing its eggshell color of laying eggs; Wherein oocyan gene heterozygote individual genotype is Oo, produce in egg the white shell egg that adulterates; Green shell homozygotic individual genotype is OO, and institute lays eggs and is green-egg-shelled;
(2) male parent is selected, adopt the method for families selecting, with the black cock chicken of old institute for male parent, its genotype is OO, Oo and oo, be that oo is hybridized with high yield white shell egg chicken, hen genotype, if institute lays eggs and is white shell in family, then this family cock is oo homozygote, do not carry oocyan gene O, eliminated; If institute's white shell of having laid eggs and green shell in family, then this family cock carries oocyan gene, and genotype is Oo, continues to employ; If lay eggs and be green shell, then this family cock carries oocyan gene, genotype is OO, continues to employ;
The production of second step, the oocyan gene individuality that isozygotys
Female parent selected by the first step and male parent are carried out mating, there are white shell egg and green-egg-shelled in institute in laying eggs, green-egg-shelled is carried out hatching and obtains F1 generation, the genotype of method to the individuality of F1 described in the first step is adopted to differentiate, filter out the cock hen individuality carrying the oocyan gene that isozygotys that hybridization is produced, white shell egg is then eliminated;
3rd step, colony expand numerous
By second step to produce and the cock with the oocyan gene OO that isozygotys identified and hen hybridize, its Offspring F2 is homozygote, realizes fast-propagation colony.
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Families Citing this family (8)

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CN105875497A (en) * 2016-03-08 2016-08-24 扬州翔龙禽业发展有限公司 Grain-saving black-feather green-shell laying hen breeding method
CN106234307B (en) * 2016-08-31 2019-03-12 湖南文妹子现代农业科技生态园有限公司 The breeding method of the black plumage Gallus domesticlus brisson of green-egg-shelled
CN107410191A (en) * 2017-06-20 2017-12-01 山东农业大学 A kind of breeding method of reed catkins feather layer of green-shell egg
CN107494410B (en) * 2017-08-18 2020-03-31 四川农业大学 Method for producing dark and light shell green-shell laying hens in matched combination manner
CN107667976B (en) * 2017-11-07 2021-06-18 山东省农业科学院家禽研究所 Method for producing seeds of male-female self-discriminating high-yield Luhua feather green-shell laying hens
CN108522421B (en) * 2018-05-02 2020-03-31 四川农业大学 Seed production method of three-line matched black feather black skin green shell laying hens
CN109006691A (en) * 2018-10-24 2018-12-18 广西万寿谷投资集团股份有限公司 A kind of breeding method of high yield Donglan Gallus domesticlus brisson
CN112088840A (en) * 2020-09-18 2020-12-18 贵州大学 Breeding method of green-shell laying hens

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