CN110419506A - Red plumage chicken produces the corss combination method of rouge and powder shell egg - Google Patents
Red plumage chicken produces the corss combination method of rouge and powder shell egg Download PDFInfo
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- 241000287828 Gallus gallus Species 0.000 title claims abstract description 89
- 239000000843 powder Substances 0.000 title claims abstract description 45
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000008774 maternal effect Effects 0.000 claims abstract description 60
- 230000008775 paternal effect Effects 0.000 claims abstract description 28
- 210000003746 feather Anatomy 0.000 claims description 28
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 230000035772 mutation Effects 0.000 claims description 14
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- 238000007400 DNA extraction Methods 0.000 claims description 11
- 238000012408 PCR amplification Methods 0.000 claims description 11
- 210000000349 chromosome Anatomy 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 claims description 6
- 108091008146 restriction endonucleases Proteins 0.000 claims description 6
- 230000029087 digestion Effects 0.000 claims description 5
- 238000012163 sequencing technique Methods 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 2
- 108010000912 Egg Proteins Proteins 0.000 abstract description 25
- 102000002322 Egg Proteins Human genes 0.000 abstract description 25
- 210000003278 egg shell Anatomy 0.000 abstract description 25
- 235000013601 eggs Nutrition 0.000 description 50
- 230000012447 hatching Effects 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 6
- 230000013011 mating Effects 0.000 description 6
- 206010064571 Gene mutation Diseases 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000002773 nucleotide Substances 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 241000272496 Galliformes Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000577218 Phenes Species 0.000 description 1
- 241000218636 Thuja Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000005549 deoxyribonucleoside Substances 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
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Abstract
The present invention relates to a kind of corss combination method that red plumage chicken produces rouge and powder shell egg, includes the following steps: that the red hen of the red cock of step S1: Yi Luodao and Lip river island is that male parent paternal line and male parent are maternal, cultivate the red cock in male parent Lip river island；Step S2: it is that maternal paternal and female parent is maternal with Lip river island Baigong chicken and Bai Lai aircraft carrier chicken, cultivates maternal；Step S3: commercial generation laying hen is cultivated with the red cock in male parent Lip river island and maternal band color spot hen, rouge and powder egg new varieties is obtained, Down co1or sexing is realized by plumage color, wherein red plumage is hen, and non-red plumage is cock.Commercial generation hen eggshell color of the present invention is rouge and powder color, plumage color is red plumage, the blank of the such layer breed type in China can be filled up, and since eggshell color is between brown shell egg and powder shell egg, further enrich domestic laying hen eggshell color type, it accords with the demands of the market, has broad application prospects.
Description
Technical field
The present invention relates to laying hen new varieties mating breeding technology fields, and in particular to a kind of red plumage chicken produces the miscellaneous of rouge and powder shell egg
Hand over matching method.
Background technique
It is influenced by Traditional consumption habits, the egg of Chinese urban and rural residents is consumed mainly based on fresh egg, the eggshell of egg
Color is paid close attention to by consumer.Powder shell egg proportion is in continue ascendant trend in the market in recent years, close to 40%.
Currently, produce the high yield commodity egg of powder shell egg mainly by brown shell layer and white shell egg chicken be orthogonal or reciprocal cross is mating and gives birth to,
Eggshell color between brown shell and white shell (L value whole process average value is 80 or so).With the increase of the brand egg market demand,
Consumer rises year by year to the hobby of rouge and powder shell egg, and this eggshell color is deeper than powder shell egg color, but than brown shell egg color
Shallowly.In existing powder shell layer chicken breed system, brown shell layer blood relationship and white shell egg chicken blood relationship respectively account for 50%, by mating to powder shell layer chicken
The breeding of brown shell strain eggshell color can deepen commercial generation egg pink colour degree to a certain extent in system.
Meanwhile red plumage rejected layer (being commonly called as old hen) has the advantages that easily sale, price are high, consumption market also has it
There is preference, so layer breed is favored by culturist, however, lacking the product that this kind of red plumage chicken produces rouge and powder shell egg currently on the market
Kind, also, the eggshell color genetic force of laying hen is medium, and Breeding Progress is slow, ineffective；Therefore, it is badly in need of a kind of method, to refer to
It leads and cultivates the new varieties that red plumage chicken produces rouge and powder egg.
Summary of the invention
To solve the shortcomings of the prior art, a kind of laying hen new varieties that can produce rouge and powder color egg, the present invention are provided
A kind of corss combination method of red plumage chicken production rouge and powder shell egg is provided, is included the following steps:
The red hen of the red cock of step S1: Yi Luodao and Lip river island is that male parent paternal line and male parent are maternal, cultivates the red cock in male parent Lip river island；
Step S2: it is that maternal paternal and female parent is maternal with Lip river island Baigong chicken and Bai Lai aircraft carrier chicken, cultivates maternal；Wherein, Luo Daobai
Cock character is presented recessive white feather, fast plumage and brown shell egg, it is white come aircraft carrier chicken character that dominant white plumage, plumage color gene homozygosis is presented is prominent
Change, slow plumage, white shell egg realize the Down co1or sexing of Lip river island Baigong chicken and Bai Lai aircraft carrier chicken offspring by Feathering type, to filter out
Maternal band color spot hen；
Step S3: commercial generation laying hen is cultivated with the red cock in male parent Lip river island and maternal band color spot hen, rouge and powder egg new varieties is obtained, passes through
Plumage color realizes Down co1or sexing, wherein red plumage is hen, and non-red plumage is cock.
Wherein, the red hen of the red cock in Lip river island and Lip river island that the male parent is paternal and male parent is maternal, character present red plumage,
Fast plumage and brown shell egg.
Wherein, the maternal paternal Lip river island Baigong chicken, selected from it is white come aircraft carrier chicken giblets hand over after hen have rust
The strain of color spot.
Wherein, the maternal paternal Lip river island Baigong chicken corresponds to the screening technique of strain are as follows: to existing Lip river island Baigong chicken
Strain carries out DNA extraction, PCR amplification and product sequencing analysis, selects the 18288303rd deoxidation core of No. 11 chromosome positive-sense strands
The strain of thuja acid homozygous mutation.
Wherein, the maternal paternal Lip river island Baigong chicken corresponds to the screening technique of strain are as follows: to existing Lip river island Baigong chicken
Strain carries out DNA extraction, PCR amplification, and to identify that sequence carries out digestion for the restriction endonuclease of T ↓ CGCGA, selection can generate two
The red feather genes homozygous mutant line of bar segment.
Wherein, the female parent it is maternal it is white come aircraft carrier chicken screening technique are as follows: to it is existing it is white come aircraft carrier chicken strain carry out
DNA extraction, PCR amplification and product sequencing analysis, select the 18288303rd deoxynucleotide homozygosis of No. 11 chromosome positive-sense strands
The strain of mutation.
Wherein, the female parent it is maternal it is white come aircraft carrier chicken screening technique are as follows: to it is existing it is white come aircraft carrier chicken strain carry out
DNA extraction, PCR amplification, and to identify that restriction endonuclease of the sequence for T ↓ CGCGA carries out digestion, selection can generate two bar segments
Red feather genes homozygous mutant line.
Red plumage chicken provided by the invention produces the corss combination method of rouge and powder shell egg, and commercial generation hen eggshell color is rouge and powder
Color, plumage color are red plumage, can fill up the blank of the such layer breed type in China, and due to eggshell color between brown shell egg and
Between powder shell egg, domestic laying hen eggshell color type is further enriched, is accorded with the demands of the market, before wide application
Scape.
Detailed description of the invention
Fig. 1: red plumage chicken of the invention produces the mating flow chart of the corss combination method of rouge and powder shell egg.
Specific embodiment
In order to have further understanding to technical solution of the present invention and beneficial effect, it is described in detail with reference to the accompanying drawing
Technical solution of the present invention and its beneficial effect of generation.
Fig. 1 is the mating flow chart for the corss combination method that red plumage chicken of the invention produces rouge and powder shell egg, as shown in Figure 1, this
The red plumage chicken that invention provides produces the corss combination method of rouge and powder shell egg, mainly includes the following steps:
1, the cultivation of male parent
It is maternal as male parent paternal line and male parent using the red hen of the red cock in Lip river island and Lip river island, cultivate the red cock in male parent Lip river island, the red public affairs in Lip river island
Red plumage, fast plumage and brown shell egg is presented in the island Ji Jiluo its character of red hen, and the red hen of the red cock in Lip river island and Lip river island is in the market
Existing common kind.
2, maternal cultivation
Using Lip river island Baigong chicken (recessive white feather, fast plumage and brown shell egg) as maternal paternal, it is white come aircraft carrier chicken (dominant white plumage, plumage color
Homozygous mutation, slow plumage, white shell egg) it is maternal as female parent, according to filial generation hen feather color, screen qualified
Lip river island Baigong chicken is as maternal paternal.
Specifically, Lip river island Baigong chicken (recessive white feather, fast plumage and brown shell egg) and it is white come aircraft carrier chicken (dominant white plumage, plumage color base
Because of homozygous mutation, slow plumage, white shell egg) after hybridization, slow plumage is presented for cock in parent, and two different phenes are presented in hen:
Band color spot hen (fast plumage, powder shell egg) and pure white feather hen (fast plumage, powder shell egg).Inventor is by above two different manifestations character
Hen hybridize with the red cock in Lip river island after find, after pure white feather hen hybridizes with the red cock in Lip river island, the laying hen of commercial generation can not be real
Existing plumage color Down co1or sexing, and after hybridizing with color spot hen with the red cock in Lip river island, the laying hen of commercial generation can be realized plumage color male and female certainly
Not.Therefore the present invention selects the female parent with color spot hen as parent's generation, selects filial generation hen that the Lip river of colored plaque-like is presented
Island Baigong chicken is as maternal paternal.
In the present invention, the method for a specific maternal paternal Lip river island Baigong chicken of screening is for example are as follows: utilizes Lip river island Baigong
Chicken (fast plumage, recessive white feather, eggshell color be brown shell) with plumage color gene homozygous mutation it is white come aircraft carrier chicken (slow plumage, dominant white plumage,
Eggshell color is white, demonstrate,proves word the 80th from No. 6 laying hen new breed system (agriculture 09) new varieties of capital powder) maternal maternal) hybridization, it presses
According to male and female ratio 1:5 artificial insemination, 10 days hatching eggs, family half sibs hatching and nestling are continuously collected, filial generation hen wears wing number,
Pedigree information is recorded, raising to 5 week old observes feather color, if the offspring hen feather of certain cock has rust color
This cock is then elected to be maternal paternal line by spot (position where color spot includes wing, back, neck etc.).
In the present invention, the meaning of maternal paternal line Lip river island Baigong's chicken recessive white feather character is: recessive white feather character is presented
Lip river island Baigong chicken, filial generation are likely to occur other non-white plumage phenotypes.In the present invention, maternal paternal and maternal maternal correspondence
Lip river island Baigong chicken and it is white come aircraft carrier chicken, be the red feather genes homozygous mutation kind selected from current existing kind,
It all has homozygous red plumage mutated gene in vivo, this gene can make the hen of its filial generation be band color spot hen.
Inventor filters out do not have plumage respectively according to Lip river island Baigong chicken and the white Apparent character come aircraft carrier chicken filial generation
The Lip river island Baigong chicken and Bai Lai aircraft carrier chicken of color gene mutation, and, have Lip river island Baigong chicken and the Bai Laihang of plumage color gene mutation
Hen finds have the corresponding product of plumage color gene mutation after carrying out DNA extraction, PCR amplification and product sequencing analysis to it respectively
System, there are homozygous mutations for the 18288303rd deoxynucleotide of No. 11 chromosome positive-sense strands.
Inventor further on its basis, has developed for the 18288303rd deoxyribonucleoside of No. 11 chromosome positive-sense strands
The restriction endonuclease of acid, identification sequence are T ↓ CGCGA, and individual corresponding two nucleotide chains of red feather genes homozygous mutation can be complete
Slitting-up generates two kinds of segments with different length；Corresponding two nucleotide chains of individual for not having red feather genes mutation are equal
It can not be cut open, still retain a kind of segment of length；Individual corresponding two nucleotide chains, one energy of red feather genes heterozygous mutant
Enough be cut open, another cannot, generate the segment of three kinds of different lengths.
Therefore, one quickly screening is maternal paternal and maternal maternal Lip river island Baigong chicken and it is white come aircraft carrier chicken method
Are as follows: to existing Lip river island Baigong chicken or it is white come aircraft carrier chicken strain carry out DNA extraction, PCR amplification and product sequencing analysis, select 11
The strain of number the 18288303rd deoxynucleotide homozygous mutation of chromosome positive-sense strand；Alternatively, to existing Lip river island Baigong chicken or
It is white come aircraft carrier chicken strain carry out DNA extraction, PCR amplification, and with identify sequence for T ↓ CGCGA restriction endonuclease carry out digestion, selection
The red feather genes homozygous mutant line of two bar segments can be generated.
3, breed system constructs
It continues from the preceding paragraph described, by dialogue come the screening of the red feather genes homozygous mutant line of the island aircraft carrier Ji Jiluo Baigong chicken, structure
Build out the new breed system of rouge and powder egg laying hen.
Male parent is maternal and male parent it is paternal be the red plumage chicken in Lip river island, the parent after hybridization is that produce the brown island Ke Danluo red
Chicken.
It is maternal paternal and it is maternal it is maternal be respectively Lip river island Baigong chicken and it is white come aircraft carrier chicken, female generation after hybridization produces powder shell
Egg, band color spot hen are in fast plumage, and cock is in slow plumage, can realize Down co1or sexing by Feathering type.
Commercial generation produces rouge and powder shell egg after the red cock in parent Lip river island and female generation band color spot hen are mating, and hen is in red plumage, cock
In non-red plumage, Down co1or sexing can be realized by plumage color.
A concrete mode of the invention is as follows:
1, breed system pure-line selection
(1) male parent line: the red chicken in Lip river island, red plumage, fast plumage, the brown shell of eggshell color.
(2) maternal maternal: Huadu Yukou Fowls Industry Co., Ltd. combines the capital cultivated with China Agricultural University
No. 6 new breed systems of laying hen of powder ((agriculture 09) new varieties demonstrate,prove word the 80th) maternal maternal chicken (specific screening technique institute for example above
State), white Leghorn, dominant white plumage, plumage color gene homozygous mutation, slow plumage, eggshell color are white shell.
(3) maternal paternal: the white laying hen in Lip river island (the specific screening side that Huadu Yukou Fowls Industry Co., Ltd. cultivates
Method is as described above), which is recessive white feather, fast plumage, and eggshell color is brown shell.White laying hen can also pass through offspring on Lip river island
Phenotypic Selection:, male and female ratio 1:5 maternal with the strain cock individual semen deposition female parent, continuous to collect hatching egg 10 days, family half sibs
Hatching and nestling, all filial generation hens wear wing number, record pedigree information.Filial generation hen is raised to 5 week old, observation
Feather color, if the offspring hen feather of certain cock has rust color spot, (position where color spot includes wing, back
Portion, neck etc.), then using this cock as maternal paternal.
2, corss combination
(1) by above-mentioned maternal paternal and maternal maternal hybridization acquisition parent godmother chicken, according to chick feather growth when hatching and nestling
Speed distinguishes male and female chicken, wherein slow plumage is cock, eliminates；Fast plumage is hen, and Feathering type identifies male and female chicken accuracy rate and exists
99.5% or more；One 68 week old of parent godmother chicken can produce qualified hatching egg 280 or more, provide strong female young number 120 or more,
Hatching egg eggshell color is powder shell；Healthy parent godmother fowl raising is selected and remain, 130 days start to lay eggs, and reach peak of laying eggs within 160 days, can
To breed.
(2) with the red cock in above-mentioned Lip river island and hen hybridization production parent for cock.
(3) according to male and female ratio 1:80, parent is bred for cock and hen, hatching and nestling obtains commercial generation laying hen, plumage color
Automatic sexing, cock are non-red plumage, and hen is red plumage, identify 98% or more accuracy rate.
Commercial generation laying hen eggshell color is rouge and powder color, and eggshell color is measured using spectral photometric colour measuring meter CM700d, and laying period is complete
Journey eggshell color L value average value 72 or so, is significantly deeper than existing high yield powder shell layer chicken eggshell color, but than brown shell egg eggshell
It is of light color, i.e., between brown shell egg and powder shell egg；
Commercial generation laying hen feather color is mainly red, and the apex of the wing and tail point are white.
80 week old feeding day egg number of commercial generation hen can raise whole 95% or more survival rate, belong to height up to 375
Laying hen breed system.
Beneficial effects of the present invention are as follows:
1, commercial generation hen eggshell color is rouge and powder color, and plumage color is red plumage, has filled up the sky of the such layer breed type in China
It is white.
2, commercial generation hen eggshell color further enriches state laying hen between brown shell egg and powder shell egg
Eggshell color type, accords with the demands of the market, and has broad prospect of application.
3, plumage speed automatic sexing can be achieved in generation in parent, and commercial generation can realize automatical sexing on feather color, reduces and turns over anus identification pair
The injury of chick can guarantee chick quality.
Although the present invention is illustrated using above-mentioned preferred embodiment, the protection model that however, it is not to limit the invention
It encloses, anyone skilled in the art are not departing within the spirit and scope of the present invention, and opposite above-described embodiment carries out various changes
It is dynamic still to belong to the range that the present invention is protected with modification, therefore protection scope of the present invention subjects to the definition of the claims.
Claims (7)
1. a kind of corss combination method that red plumage chicken produces rouge and powder shell egg, which comprises the steps of:
The red hen of the red cock of step S1: Yi Luodao and Lip river island is that male parent paternal line and male parent are maternal, cultivates the red cock in male parent Lip river island；
Step S2: it is that maternal paternal and female parent is maternal with Lip river island Baigong chicken and Bai Lai aircraft carrier chicken, cultivates maternal；Wherein, Luo Daobai
Cock character is presented recessive white feather, fast plumage and brown shell egg, it is white come aircraft carrier chicken character that dominant white plumage, plumage color gene homozygosis is presented is prominent
Change, slow plumage, white shell egg realize the Down co1or sexing of Lip river island Baigong chicken and Bai Lai aircraft carrier chicken offspring by Feathering type, to filter out
Maternal band color spot hen；
Step S3: commercial generation laying hen is cultivated with the red cock in male parent Lip river island and maternal band color spot hen, rouge and powder egg new varieties is obtained, passes through
Plumage color realizes Down co1or sexing, wherein red plumage is hen, and non-red plumage is cock.
2. the corss combination method that red plumage chicken as described in claim 1 produces rouge and powder shell egg, which is characterized in that the male parent is paternal
And red plumage, fast plumage and brown shell egg is presented in the red hen of the red cock in Lip river island and Lip river island that male parent is maternal, character.
3. the corss combination method that red plumage chicken as described in claim 1 produces rouge and powder shell egg, which is characterized in that described maternal paternal
Lip river island Baigong chicken, selected from it is white come aircraft carrier chicken giblets hand over after hen have the strain of rust color spot.
4. the corss combination method that red plumage chicken as claimed in claim 3 produces rouge and powder shell egg, which is characterized in that described maternal paternal
Lip river island Baigong chicken correspond to the screening technique of strain are as follows: to existing Lip river island Baigong chicken strain carry out DNA extraction, PCR amplification and
Product sequencing analysis selects the strain of the 18288303rd deoxynucleotide homozygous mutation of No. 11 chromosome positive-sense strands.
5. the corss combination method that red plumage chicken as claimed in claim 3 produces rouge and powder shell egg, which is characterized in that described maternal paternal
Lip river island Baigong chicken correspond to the screening technique of strain are as follows: DNA extraction, PCR amplification are carried out to existing Lip river island Baigong chicken strain, and
To identify that restriction endonuclease of the sequence for T ↓ CGCGA carries out digestion, selection can generate the red feather genes homozygous mutation product of two bar segments
System.
6. the corss combination method that red plumage chicken as described in claim 1 produces rouge and powder shell egg, which is characterized in that the female parent is maternal
It is white come aircraft carrier chicken screening technique are as follows: to it is existing it is white come aircraft carrier chicken strain carries out DNA extraction, PCR amplification and product are sequenced
Analysis, selects the strain of the 18288303rd deoxynucleotide homozygous mutation of No. 11 chromosome positive-sense strands.
7. the corss combination method that red plumage chicken as described in claim 1 produces rouge and powder shell egg, which is characterized in that the female parent is maternal
It is white come aircraft carrier chicken screening technique are as follows: to it is existing it is white come aircraft carrier chicken strain carry out DNA extraction, PCR amplification, and to identify sequence
The restriction endonuclease for being classified as T ↓ CGCGA carries out digestion, and selection can generate the red feather genes homozygous mutant line of two bar segments.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111345267A (en) * | 2020-04-08 | 2020-06-30 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN111406712A (en) * | 2020-05-22 | 2020-07-14 | 南昌师范学院 | Method for cultivating high-quality chicken with both colored feather and eggs by using babcock B380 |
| CN114667965A (en) * | 2022-02-17 | 2022-06-28 | 江苏省家禽科学研究所科技创新有限公司 | Method for breeding high-reproduction, high-efficiency and high-quality dual-purpose black-feather chickens |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111345267A (en) * | 2020-04-08 | 2020-06-30 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN111345267B (en) * | 2020-04-08 | 2021-11-19 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Breeding method of hybrid line of special laying hens with feather color double-self-distinguishing male and female black-feather pink shells and high yield |
| CN111406712A (en) * | 2020-05-22 | 2020-07-14 | 南昌师范学院 | Method for cultivating high-quality chicken with both colored feather and eggs by using babcock B380 |
| CN114667965A (en) * | 2022-02-17 | 2022-06-28 | 江苏省家禽科学研究所科技创新有限公司 | Method for breeding high-reproduction, high-efficiency and high-quality dual-purpose black-feather chickens |
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| CN110419506B (en) | 2022-01-14 |
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Denomination of invention: Hybrid matching method of red-feather chicken for producing red-pink shell eggs Effective date of registration: 20220818 Granted publication date: 20220114 Pledgee: Agricultural Bank of China Limited Beijing Pinggu sub branch Pledgor: BEIJING HUADU YUKOU POULTRY INDUSTRY Co.,Ltd. Registration number: Y2022110000180 |
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Denomination of invention: A Hybrid Matching Method for Red Feather Chicken to Produce Red Powder Shell Eggs Effective date of registration: 20231130 Granted publication date: 20220114 Pledgee: Agricultural Bank of China Limited Beijing Pinggu sub branch Pledgor: BEIJING HUADU YUKOU POULTRY INDUSTRY Co.,Ltd. Registration number: Y2023110000501 |