CN101411313A - Method for producing fish seeds of genetic male red tilapia hybrids - Google Patents
Method for producing fish seeds of genetic male red tilapia hybrids Download PDFInfo
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- CN101411313A CN101411313A CNA2008102193944A CN200810219394A CN101411313A CN 101411313 A CN101411313 A CN 101411313A CN A2008102193944 A CNA2008102193944 A CN A2008102193944A CN 200810219394 A CN200810219394 A CN 200810219394A CN 101411313 A CN101411313 A CN 101411313A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for producing genetically male red tilapia hybrid fingerlings, which belongs to the technical field of breeding tilapia. A main point of a technical proposal of the method is to adopt red female tilapia and YY-type supermale tilapia nilotica for hybridizing propagation, wherein the red female tilapia is bred via more than 10 years of purification and rejuvenation after the red female tilapia was introduced from Taiwan in 1995; and the YY-type supermale tilapia nilotica is bred by use of a YY/GMT technique. The male red tilapia hybrid fingerlings produced by the method have the advantages of high red rate and male rate. The method can provide a brand new breeding technique for culturing red tilapia.
Description
Technical field
The present invention relates to the breeding technique of a kind of XX ♀-XY ♂ type Tilapia mossambica, more particularly, it relates to the producing method for seed of the male red Luo Fei hybrid fish of a kind of heredity.
Background technology
Tilapia mossambica is wide with its delicious meat, feeding habits, breeding is fast, adaptability and the strong advantage of premunition, and all there is raising in a lot of in the world countries; After the Tilapia mossambica kind was introduced from the sixties in 20th century in the China mainland area, the breed of Tilapia mossambica just obtained bigger development in the later stage eighties 20th century, and the overall annual production in the whole nation at present surpasses 1,200,000 tons, has become one of main economic freshwater fish.
In the Tilapia mossambica kind of culturing, there is a kind of red tilapia to be commonly called as the rainbow porgy, be find in Taiwan first, it is actually separates from red Mozambique Tilapia mossambica and bolti filial generation.Because it has the body colour bright red, salt resistance is strong, fertility is high, raise easily and the high advantage of the market price, become the well received breed object in China, Southeast Asia and European and American areas.At present, on China red tilapia seed produces, mostly adopt red sieve fish self propagated, its milter rate is common about 50%, and its filial generation contains 5～25% black seedling, in the process of selling the red tilapia seedling, choose black seedling superseded, so just increase seed breeding and labour cost, therefore how to have grasped color inheritance rule, utilization hybrid vigour and property control techniques, the complete fast male red Luo Fei hybrid fish of exploitation growth is applied to produce, and has become one of important topic of aquatic products industry tackling of key scientific and technical problems.
Summary of the invention
The technical problem to be solved in the present invention is the above-mentioned deficiency at prior art, and the producing method for seed of all high hereditary male red Luo Fei hybrid fish of a kind of red rate and male ratio is provided.
Technical scheme of the present invention is such: the producing method for seed of the male red Luo Fei hybrid fish of a kind of heredity is to adopt red tilapia raun and Ni Luoluofei YY type supermale fish to carry out outbreeding; The red tilapia raun is after introducing from Taiwan nineteen ninety-five, to select after purification and rejuvenation in more than 10 years; Described YY type supermale bolti brings out with the YY/GMT technology.
In the producing method for seed of the male red Luo Fei hybrid fish of heredity of the present invention, described outbreeding is to adopt single female and single male breeding to breed.
The present invention has compared with prior art solved the black fry problem that the body colour breeding aspect in the cultivating red tilapia exists, and can guarantee to hybridize fry is whole red; Simultaneously, improving aspect the male ratio of fry, improving greatly than about 50% male ratio in the red Tilapia mossambica self propagated of tradition, its male ratio can reach more than 90%, for the breed of red Tilapia mossambica provides a kind of brand-new technology, improves culture benefit greatly.
Embodiment
Below in conjunction with embodiment the inventor is described in further detail, but does not constitute any limitation of the invention.
Embodiment 1
1. materials and methods
1.1 material
1.1.1 Tilapia mossambica fingerling source: experiment original seed red tilapia is after introducing from Taiwan nineteen ninety-five, to select after purification and rejuvenation in surplus in the of 10 years, and its sex mechanism is XX ♀-XY ♂ type; YY type ultra-male Tilapia is that the inventor is supporting method with two, existing is referred to as in the world that the YY/GMT technology brings out, and its sex mechanism is YY ♂ type.
1.1.2 experimental facilities: mouth surplus the water Buddhist nun pond 20,, long * wide * dark: 300cm * 300cm * 120cm, with A, B ... numbering; Plumbing, oxygenation facility etc.
1.2 method
1.2.1 red tilapia raun and YY type supermale bolti cross method are adopted in this experiment in 2007.
1.2.2 mainly adopt single female and single male breeding method to breed, every group breeds filial generation and carries out the cultivation of single pond.
1.2.3 the body colour of fish in the seed developmental stage, just displays, and is main by redness, pink and wild type body colour (abbreviation black) division.Should illustrate, black splotch can occur on the trunk of a lot of red fishes and the position of fin, but still be considered to red fish on the whole.Above color classification is pressed in filial generation to each combination, and numeration respectively.
1.2.4 in order to study the genetic development of the red body colour of Tilapia mossambica, people propose the hereditary pattern of red body colour, promptly in the red tilapia strain of Taiwan, the hereditary pattern that the someone supposes is two allelic incomplete dominances on the site; In the Mozambique Tilapia mossambica, redness is seemingly controlled by single recessive gene on the different loci.
1.2.5, mainly confirm according to dissecting fish body observation ovary or spermary to the sex identification of experiment fish.
1.2.6 experimental result is carried out statistics and genetic analysis.
2. experimental result and analysis
2.1 Tilapia mossambica color inheritance result and analysis
2.1.1 color inheritance result
In 8 combinations of red tilapia raun and the hybridization of YY type supermale bolti, wherein the filial generation of 7 combinations is 100% red fish, and the red fish of 1 combination filial generation and the ratio of wild body colour fish are 1:1.Now list observed result in table 1
Table 1 hybrid Tilapia mossambica body colour observed result
*
*R ♀ (xx) * N ♂ (yy) means red tilapia raun and the mating of YY type supermale bolti
2.1.2 analyze
Adopt from us in 8 combination experiments of red sieve raun and the hybridization of YY type Buddhist nun Luo Chao milter, have the filial generation of 7 combinations to be red fish; Have only the filial generation of a combination, red fish and black fish respectively account for about 50%.How to explain this genetic phenomenon? we think that the filial generation of 7 combinations is redness, and this explanation our company red tilapia body colour is purer, but the impure person of minority is also arranged, so the black fish just occurred.This can make following hereditary pattern and represent: 1, RR ♀ (pink) * rr ♂ (black) → filial generation Rr is 100% redness.2, the redness of red R r ♀ * black rr ♂ → filial generation Rr+rr and black ratio are 1:1.3, purebred red sieve RR ♀ * RR ♂ → filial generation RR shows as whole red.4, purebred Buddhist nun sieve and Lan Luo rr ♀ * rr ♂ → filial generation rr shows as all black.
Above genetic analysis explanation, the color inheritance of the red tilapia of our company is to carry out with the term single gene mode of inheritance of incomplete dominance.Here, the red proterties of red tilapia is a dominance, and the proterties of wild type (black) is recessive.These are different with the hypothesis that the people is arranged.
In addition, we also carry out red sieve raun (15 tail) and Buddhist nun sieve milter (10 tail) mass mating experiment, sampling filial generation 2426 tails.Wherein red fish accounts for 88.8%, and non-red fish accounts for 11.2%.This experimental result, same available above-mentioned hereditary pattern explain, and red sieve raun parent 90% of explanation our company be purebred (RR), but also exist 10% parent (Rr) needs superseded.
2.2 Tilapia mossambica sex heredity result and analysis
2.2.1 sex observed result
In order to improve the male ratio of hybrid red tilapia, existing again with above-mentioned hybrid experiment, the sex observed result of filial generation is listed table 2 in.
The other ration statistics of the red sieve hybrid of table 2 piscinity
*
| Pond number | The experiment combination | Specification (millimeter) | Sum | The raun number | The milter number | ♂％ | Genotype |
| G-11 | R♀(xx)×N♂(yy) | 109～145 | 36 | 4 | 32 | 88.9 | XY |
| G-15 | R♀(xx)×N♂(yy) | 102～119 | 63 | 4 | 59 | 93.7 | XY |
| H-1 | R♀(XX)×N♂(YY) | 64～82 | 42 | 1 | 41 | 97.6 | XY |
| H-2 | R♀(XX)×N♂(YY) | 47～56 | 30 | 5 | 25 | 83.3 | XY |
| T-1/4 | R♀(XX)×N♂(YY) | 135～165 | 42 | 4 | 38 | 90.5 | XY |
| T-2/4 | R♀(XX)×N♂(YY) | 112～125 | 69 | 20 | 49 | 71.0 | XY |
| T-1/3 | R♀(XX)×N♂(YY) | 100～123 | 57 | 3 | 54 | 94.7 | XY |
| W-3 | R♀(XX)×N♂(YY) | 129 | 2 | 127 | 98.4 | XY |
*R ♀ (xx) * N ♂ (yy) means red tilapia raun and the mating of YY type supermale bolti
2.1.2 analyze
As can be seen from Table 2, adopt red tilapia raun (XX ♀) and Luo Fei supermale fish (YY ♂) breeding, can obtain 100% XY type red Luo Fei hybrid fish (XY), the male ratio average out to 90% of its performance.Remove that T-2/4 pond situation is special to be disregarded, then male ratio is 92.4%.The male fish of red Luo Fei hybrid (XY) comparison has significantly complete male growth vigor according to red sieve fish (XX ♀+XY ♂).
Claims (2)
1. the producing method for seed of the male red Luo Fei hybrid fish of heredity is characterized in that, is to adopt red tilapia raun and Ni Luoluofei YY type supermale fish to carry out outbreeding; The red tilapia raun is after introducing from Taiwan nineteen ninety-five, to select after purification and rejuvenation in more than 10 years; Described YY type supermale bolti brings out with the YY/GMT technology.
2. the producing method for seed of the male red Luo Fei hybrid fish of heredity according to claim 1 is characterized in that, described outbreeding adopts single female and single male breeding to breed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430084A (en) * | 2014-11-25 | 2015-03-25 | 中国水产科学研究院淡水渔业研究中心 | Family breeding method for pearl white strain red tilapia |
| CN105145438A (en) * | 2015-09-30 | 2015-12-16 | 中国水产科学研究院淡水渔业研究中心 | Method for establishing nile tilapia merit female pure line |
| CN105724290A (en) * | 2016-03-02 | 2016-07-06 | 广西壮族自治区水产引育种中心 | Method for selecting red tilmpa parent directionally and constructing core group |
| CN113637764A (en) * | 2021-05-14 | 2021-11-12 | 中山大学 | Detection primer for microsatellite related to body color of tilapia and application of detection primer |
-
2008
- 2008-11-25 CN CN2008102193944A patent/CN101411313B/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430084A (en) * | 2014-11-25 | 2015-03-25 | 中国水产科学研究院淡水渔业研究中心 | Family breeding method for pearl white strain red tilapia |
| CN104430084B (en) * | 2014-11-25 | 2016-06-29 | 中国水产科学研究院淡水渔业研究中心 | A kind of family selective breeding method of pearl white strain red tilapia |
| CN105145438A (en) * | 2015-09-30 | 2015-12-16 | 中国水产科学研究院淡水渔业研究中心 | Method for establishing nile tilapia merit female pure line |
| CN105145438B (en) * | 2015-09-30 | 2017-12-01 | 中国水产科学研究院淡水渔业研究中心 | The method for establishing bolti merit female pure lines |
| CN105724290A (en) * | 2016-03-02 | 2016-07-06 | 广西壮族自治区水产引育种中心 | Method for selecting red tilmpa parent directionally and constructing core group |
| CN113637764A (en) * | 2021-05-14 | 2021-11-12 | 中山大学 | Detection primer for microsatellite related to body color of tilapia and application of detection primer |
| CN113637764B (en) * | 2021-05-14 | 2023-05-30 | 中山大学 | Detection primer of microsatellite with tilapia body color and application thereof |
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| CN101411313B (en) | 2012-05-16 |
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Assignee: Hunan Dongjianghu Fishery Co., Ltd. Assignor: Guangzhou Luye Aquatic Products Co., Ltd. Contract record no.: 2012430000141 Denomination of invention: Method for producing fish seeds of genetic male red tilapia hybrids Granted publication date: 20120516 License type: Exclusive License Open date: 20090422 Record date: 20120618 |
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Owner name: XIAMEN LUYE FISHERIES CO., LTD. Free format text: FORMER OWNER: GUANGZHOU LUYE AQUATIC PRODUCTS CO., LTD. Effective date: 20121218 |
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Effective date of registration: 20121218 Address after: Jimei Zhongzhou town of Jimei District of Xiamen City, Fujian province 361000 Patentee after: Xiamen Heron aquatic products Co., Ltd. Address before: 510860 Guangdong province Guangzhou City Shiling Town Huadu District, Furong Village Special Road No. 88 on the third. Patentee before: Guangzhou Luye Aquatic Products Co., Ltd. |
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Effective date of registration: 20160226 Address after: Changtai County eco-tourism areas of mayangxi mountain village 363900 Fujian city of Zhangzhou Province Patentee after: Changtai Shengyuan Bacera Aquatic Seed Co. Ltd. Address before: Jimei Zhongzhou town of Jimei District of Xiamen City, Fujian province 361000 Patentee before: Xiamen Heron aquatic products Co., Ltd. |