CN101406165B - Method for producing seeds of ultra-male Niao tilapia - Google Patents
Method for producing seeds of ultra-male Niao tilapia Download PDFInfo
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- CN101406165B CN101406165B CN2008102193959A CN200810219395A CN101406165B CN 101406165 B CN101406165 B CN 101406165B CN 2008102193959 A CN2008102193959 A CN 2008102193959A CN 200810219395 A CN200810219395 A CN 200810219395A CN 101406165 B CN101406165 B CN 101406165B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for producing seeds of supermale Nero-Aurea tilapia, which belongs to the technical field of breeding tilapia. A technical proposal of the method has technical key point of mating WZ female-ZZ male type physiological female oreochromis aurea (ZZ delta female) with XX female-XY male type Nero supermale tilapia (YY male) so as to produce Nero-Austrian genetic supermale tilapia (YZ male). The super tilapia bred by the method has great significance for healthily breeding, improving male rate (100 percent) and promoting tilapia culture technology.
Description
Technical field
The present invention relates to a kind of Java tilapia breeding technology, more particularly, it relates to the method that the technology of feminizing of utilizing WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote is carried out the supermale Buddhist nun Tilapia mossambica production of hybrid seeds difficult to understand.
Background technology
Tilapia mossambica is wide with its delicious meat, feeding habits, breeding is fast, adaptability and the strong advantage of premunition, and all there is raising in a lot of in the world countries; After the Tilapia mossambica kind was introduced from the sixties in 20th century in the China mainland area, the breed of Tilapia mossambica just obtained bigger development in the later stage eighties 20th century, and the overall annual production in the whole nation at present surpasses 1,200,000 tons, has become one of main economic freshwater fish.Wherein the Buddhist nun fish difficult to understand in the Tilapia mossambica is by Ni Luoluofei raun (XX) and Leah Luo Fei milter difficult to understand (ZZ) hybrid seeding and since it have tangible hybrid vigour on producing, used 40 surplus year.But the male ratio of this hybrid large-scale production does not far meet the aquaculture needs generally 80%～95%, influences the output and the quality of marketable fish; Many for this reason production of hybrid seeds units adopt androgenic hormone to handle fry and induce to the complete male milter of what is called, can have a strong impact on consumer health and ecotope like this.In order to improve the male ratio of Buddhist nun hybrid difficult to understand, R&D institution's work that going in for the study.People such as the appearance Shou Bai of Marine University Of Zhanjiang carry out the hybrid experiment that bolti transforms raun (△ XY ♀) and Ao Liya milter (ZZ ♂); Can obtain Buddhist nun hybrid difficult to understand and mix male fish (XZ ♂+YZ ♂); Infer because the cause that YZ type hybrid fish exists; The comparable present Buddhist nun fish difficult to understand of its male ratio improves 2.5%, makes the male fish male ratio of mixing be up to 97.5%.The present inventor is at the process years of researches; Finally, the Tilapia mossambica milter makes a breakthrough in feminizing research; And applied for Chinese patent " method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote "; On this basis, transform raun (ZZ △ ♀), invented the producing method for seed of a kind of new supermale Buddhist nun Tilapia mossambica difficult to understand according to the Leah Luo Fei difficult to understand that is obtained.
Summary of the invention
The technical problem that the present invention will solve is the above-mentioned deficiency to prior art, provides a kind of health of growing seedlings, male ratio can reach the producing method for seed of 100% supermale Buddhist nun Tilapia mossambica difficult to understand.
Technical scheme of the present invention is such: the producing method for seed of a kind of supermale Buddhist nun Tilapia mossambica difficult to understand; Be to select for use WZ ♀-male zygote of ZZ ♂ type Oreochromis aureus after feminizing, to be transformed into physiology raun (ZZ △ ♀); Then this physiology raun and XX ♀-XY ♂ type Ni Luoluofei supermale fish (YY ♂) are carried out mating, produce hereditary supermale Buddhist nun Tilapia mossambica (YZ ♂) difficult to understand.
Above-mentioned Ni Luoluofei supermale fish is that the present inventor is that supporting method brings out with two, is referred to as the YY/GMT technology in the world.
In the producing method for seed of above-mentioned supermale Buddhist nun Tilapia mossambica difficult to understand, the described processing of feminizing is that the undifferentiated Oreochromis aureus seedling of sexual gland more than 15 days, is carried out normal feeding and management with the bait feeding that contains female sex hormone then, can obtain physiology raun (ZZ △ ♀); The dosage of female sex hormone is that every gram feed contains female sex hormone 100～200 micrograms in the pharmaceutical chemistry; Water temperature during bait feeding is 25～30 ℃.
In the producing method for seed of above-mentioned supermale Buddhist nun Tilapia mossambica difficult to understand, described female sex hormone (FH) is estradiol.
The present invention compared with prior art has following advantage:
(1) male ratio mixes the highest 97.5% the improving 2.5% again and reach 100% of male fish than existing Buddhist nun hybrid difficult to understand;
(2) to mix male fish than existing Buddhist nun hybrid difficult to understand will be more remarkable to growth vigor.To have only 50% fish in the male fish be the supermale fish because mix, and the present invention is 100% supermale fish.
(3) supermale fish of the present invention does not exist existing Buddhist nun hybrid difficult to understand to mix the reproductive patterns of male fish in culturing the marketable fish process, thereby makes marketable fish size, quality, price higher, and economic benefit is better.
Embodiment
Below in conjunction with embodiment the present invention is done to specify further, but do not constitute any restriction of the present invention.
Embodiment 1
1. materials and methods
1.1 material
The experiment starting material is the bolti (o.niloticus) and Oreochromis aureus (o.aurea) breeding of our company, and the hereditary basis of its breeding and sex mechanism can be represented with NNXX ♀-NNXY ♂ and AAWZ ♀-AAZZ ♂ respectively.
1.2 method
Select for use the Ni Luoluofei supermale fish (NNYY ♂) and the Leah Luo Fei physiology raun difficult to understand (AAZZ △ ♀) of our company to breed; The optimum organization technology of specifically feminizing of Leah physiology raun difficult to understand; In other a one Chinese patent application file " method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote ", detailed introduction has been arranged, the full content of this application is all included the application's open scope in; Hybrid experiment of the present invention is at a plurality of 200m
2～300m
2Carry out in the fishpond.
The sex of hybrid is mainly confirmed according to sexual organ and sexual gland.Biological information to hybrid carries out genetic analysis.
2. experiment observed result
2.1 novel Buddhist nun hybrid sex difficult to understand is observed and statistical analysis
Experiment is carried out in the Xiamen of our company and the Duo Kou fishpond in Guangzhou, breeds the tens thousand of tails of hybrid fry altogether, will random sampling observation statistics list in the table 1 in the fishpond at present.
Possibly find out that by table 1 male ratio of each pond Buddhist nun hybrid fish difficult to understand is 100%.
Research and analyse also and show, the sex gene bion of this novel Buddhist nun hybrid fish difficult to understand is a YZ ♂ type, this with at present on producing sex gene bion of the Buddhist nun hybrid fish difficult to understand of application be that XZ ♂ type is different.
Filial generation male ratio and the genetic analysis of table 1 ZZ △ ♀ * YY ♂
2.2 interpretation of result
The Buddhist nun of breeding of the present invention hybrid difficult to understand is compared with the Buddhist nun hybrid difficult to understand that production has at present been used, and its form structure is similar not to be accidental, and this is relevant with hereditary basis.Need explanation here first, N representes the summation of gene on the bolti monoploid autosome, and A representes the summation of gene on the Oreochromis aureus monoploid autosome.When bolti (NNXX ♀) transforms raun (AAZZ △ ♀) breeding with Oreochromis aureus (AAZZ ♂) breeding and Ni Luoluofei supermale fish (NNYY ♂) with Leah Luo Fei difficult to understand; All can obtain the diplontic hybrid filial generation of NA; This shows that they have common physical basis of heredity.Research and analyse also and show; Hybrid vigour appears in Buddhist nun hybrid difficult to understand; Show that mainly economic characters are like aspects such as growth, output; Then be by numerous complementary gene results of interaction on parents' autosome, with this reasoning, the Buddhist nun hybrid difficult to understand with breeding of the present invention of NA hereditary basis also hybrid vigour can occur.In addition, these two hybrids also have a common feature, be exactly they all be parents' F1 generation, have the characteristics of " purebred ", this just determines that their can show uniformity at aspects such as form and economic characters.
Be worth particularly pointing out, though these two kinds of hybrid fishes have common hereditary basis on autosome,, be again distinguishing on sex chromosome between them.Know, the Buddhist nun hybrid difficult to understand of on producing, using, its sex physiology genotype is XZ ♂, the Buddhist nun hybrid difficult to understand of breeding of the present invention (claiming Buddhist nun hybrid supermale fish difficult to understand or super Buddhist nun hybrid difficult to understand again), the physiology genotype of its sex is YZ ♂, obviously the two is different.We think that why the Buddhist nun hybrid difficult to understand of breeding of the present invention shows male ability strong (male ratio is 100%) is relevant with two male chromosomal synergy.And appearance 5%～20% is female easily in Buddhist nun hybrid difficult to understand (XZ) colony; Making colony's male ratio is 80%～95%; This causes by XZ genotype inherent characteristic, because it only has the male chromosome of Z and is not Y chromosome, so just is difficult to guarantee not make X gene and is positioned on the autosome uncertain little effect female gene and unites the performance leading role;, this is that XZ type fish is than the relatively poor reason of the male ability of YZ type fish.
More than analyze explanation, the Buddhist nun of breeding of the present invention its hereditary basis of hybrid difficult to understand is NAYZ, and it had both inherited existing Buddhist nun hybrid difficult to understand (NAXZ ♂) the F1 hybrid vigour in generation, had 100% complete male advantage again.The super Buddhist nun of breeding of the present invention hybrid fish (NAYZ ♂) difficult to understand; Possibly be substituted with historical Buddhist nun hybrid fish difficult to understand (NAXZ ♂) decades; This grows seedlings for health; Prevent to occur unnecessary breeding in the marketable fish poud, improve the output and the quality of marketable fish, the development and the export processing that promote China's Tilapia mossambica monosex cultivation industry are all significant.
Claims (1)
1. the producing method for seed of supermale Buddhist nun Tilapia mossambica difficult to understand; It is characterized in that; Be to select for use WZ ♀-male zygote of ZZ ♂ type Oreochromis aureus after feminizing, to be transformed into the physiology raun; Then this physiology raun and XX ♀-XY ♂ type Ni Luoluofei supermale fish are carried out mating, produce hereditary supermale Buddhist nun Tilapia mossambica difficult to understand; Described Ni Luoluofei supermale fish is that supporting method brings out with two, is referred to as the YY/GMT technology in the world; The described processing of feminizing is that the undifferentiated Oreochromis aureus seedling of sexual gland more than 15 days, is carried out normal feeding and management with the bait feeding that contains female sex hormone then, can obtain the physiology raun; The dosage of female sex hormone is that every gram feed contains female sex hormone 100～200 micrograms in the pharmaceutical chemistry; Water temperature during bait feeding is 25～30 ℃; Described female sex hormone is estradiol.
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| CN2008102193959A CN101406165B (en) | 2008-11-25 | 2008-11-25 | Method for producing seeds of ultra-male Niao tilapia |
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| CN2008102193959A CN101406165B (en) | 2008-11-25 | 2008-11-25 | Method for producing seeds of ultra-male Niao tilapia |
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| CN101406165B true CN101406165B (en) | 2012-07-11 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763625A (en) * | 2012-07-28 | 2012-11-07 | 湖南农业大学 | Culture method of pseudo-female Chinese soft-shelled turtle and all-male Chinese soft-shelled turtle |
| KR101575930B1 (en) * | 2013-06-15 | 2015-12-09 | 구골홀딩스 주식회사 | Method for inducing artificial ovulation and spawn of eel |
| CN106399293B (en) * | 2016-08-24 | 2019-12-31 | 中山大学 | High-efficiency induction and microsatellite molecular marker identification method for nile tilapia pseudofemale fish |
| TWI645779B (en) * | 2017-07-21 | 2019-01-01 | 嘉豐海洋國際股份有限公司 | Induction of Nile Wu Guoyu by soaking treatment with methyl ketone Male method |
| CN107996468A (en) * | 2017-12-20 | 2018-05-08 | 淮阴师范学院 | A kind of ussuriensis Genetic male reverses physiology female fish to educate screen method |
| CN108244000A (en) * | 2018-02-08 | 2018-07-06 | 宁波市海洋与渔业研究院 | The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized |
| CN109122415B (en) * | 2018-08-01 | 2020-12-29 | 广西壮族自治区水产科学研究院 | Breeding method of high-reproduction type tilapia mossambica mating line male parent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138329A (en) * | 2007-09-30 | 2008-03-12 | 中国水产科学研究院淡水渔业研究中心 | Tilapia male and female pairing propagating method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101138329A (en) * | 2007-09-30 | 2008-03-12 | 中国水产科学研究院淡水渔业研究中心 | Tilapia male and female pairing propagating method |
Non-Patent Citations (5)
| Title |
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| C.Melard.Production of a high percentage of male offspring with 17alpha-ethynylestradiol sex-reversed Oreochromis aureus. I. Estrogen sex-reversal and production or F2 pseudofemales.《Aquaculture》.1995,第130卷(第1期),25-34. * |
| D.Desprez,C.Melard.Effect or ambient water temperature on sex determinism in the bule tilapia oreochromis aureus.《Aquaculture》.1998,第162卷(第1-2期),79-84. * |
| 万松良等.蓝非鲫（WZ）莫桑比克非鲫（YY）杂交子一代性别观察.《水产科学》.1994,第13卷(第1期),15-19. * |
| 杨景峰等.罗非鱼全雄控制技术研究进展.《内蒙古民族大学学报(自然科学版)》.2003,第18卷(第4期),318-322. * |
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Owner name: XIAMEN LUYE FISHERIES CO., LTD. Free format text: FORMER OWNER: GUANGZHOU LUYE AQUATIC PRODUCTS CO., LTD. Effective date: 20121218 |
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Effective date of registration: 20121218 Address after: Jimei Zhongzhou town of Jimei District of Xiamen City, Fujian province 361000 Patentee after: Xiamen Heron aquatic products Co., Ltd. Address before: 510860 Guangdong province Guangzhou City Shiling Town Huadu District, Furong Village Special Road No. 88 on the third. Patentee before: Guangzhou Luye Aquatic Products Co., Ltd. |
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Effective date of registration: 20160226 Address after: Changtai County eco-tourism areas of mayangxi mountain village 363900 Fujian city of Zhangzhou Province Patentee after: Changtai Shengyuan Bacera Aquatic Seed Co. Ltd. Address before: Jimei Zhongzhou town of Jimei District of Xiamen City, Fujian province 361000 Patentee before: Xiamen Heron aquatic products Co., Ltd. |