CN101449664B - Seed production method of WZ(male)-ZZ(female) all-male blue tilapia - Google Patents
Seed production method of WZ(male)-ZZ(female) all-male blue tilapia Download PDFInfo
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- CN101449664B CN101449664B CN200810219397A CN200810219397A CN101449664B CN 101449664 B CN101449664 B CN 101449664B CN 200810219397 A CN200810219397 A CN 200810219397A CN 200810219397 A CN200810219397 A CN 200810219397A CN 101449664 B CN101449664 B CN 101449664B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a method of culturing WZ(female)-ZZ(male) type Oriali java tilapia full milter, belonging to the technical field of java tilapia breeding, characterized by selecting a male fry of Oriali strain to convert into physiological hen through feminization, then mating the physiological hen with the milter of the other Oriali strain, generating genetic full milter. The inventive method has the advantages of convenient management, high fertility rate and seedling rate, good brand stability, used for breeding cultivation of Oriali java tilapia full milter.
Description
Technical field
The present invention relates to the breeding technique of WZ ♀-ZZ ♂ type Tilapia mossambica, more particularly, it relates to the producing method for seed of WZ ♀-complete male Oreochromis aureus of ZZ ♂ type.
Background technology
Tilapia mossambica is wide with its delicious meat, feeding habits, breeding is fast, adaptability and the strong advantage of premunition, and all there is raising in a lot of in the world countries; After the Tilapia mossambica kind was introduced from the sixties in 20th century in the China mainland area, the breed of Tilapia mossambica just obtained bigger development in the later stage eighties 20th century, and the overall annual production in the whole nation at present surpasses 1,200,000 tons, has become one of main economic freshwater fish.In the cultural technique of Tilapia mossambica, the fast characteristics of Tilapia mossambica breeding also become the significant drawbacks that the puzzlement Tilapia mossambica is cultured development just; Reason is the early sexual maturity of Tilapia mossambica; By traditional male and female polyculture method, cause the excessive multiplication of Tilapia mossambica easily, make the size of cultured population be difficult to control; Cause the value of product low, deficiency in economic performance.
For this reason, personnel have proposed the complete male cultural technique of Tilapia mossambica in the industry, and the source of male Tilapia mossambica, the past is normally selected milter from the male and female population mixture, thereby causes the complex management of complete male cultural technique, and the raun serious waste.In view of the foregoing, the raun that American scholar jensen in 1979 and Shelton propose the Ao Liya sex reversal and the breeding of former milter obtain the imagination of the full milter in the heredity, do not achieve success but test; The present inventor is through years of researches; Finally, Tilapia mossambica makes a breakthrough in feminizing research; And applied for Chinese patent " method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote "; On this basis, according to the needs of culturing, a kind of seeding technique of complete male Oreochromis aureus has newly been proposed.
Summary of the invention
The technical problem that the present invention will solve is the above-mentioned deficiency to prior art, and the producing method for seed of WZ ♀-complete male Oreochromis aureus of ZZ ♂ type that a kind of convenient management, fertilization rate and emergence rate are high, variety stability is good is provided.
Technical scheme of the present invention is such: a kind of producing method for seed of complete male Oreochromis aureus; Be to select for use a kind of milter of Leah strain difficult to understand after feminizing, to be transformed into physiology raun (AAZZ △ ♀); Milter (BBZZ ♂) with this physiology raun and another kind of Ao Liya strain carries out mating (AAZZ △ ♀ * BBZZ ♂) then, produces the full milter (ABZZ ♂) of heredity.
In the producing method for seed of above-mentioned WZ ♀-complete male Oreochromis aureus of ZZ ♂ type; Described Leah strain difficult to understand is a kind of to be the strain of being introduced from Israel by Taiwan (AAWZ ♀/AAZZ ♂), and another is the strain (BBWZ ♀/BBZZ ♂) that introduce from the U.S. in fresh water fishery research center, the Yangtze river basin.
In the producing method for seed of above-mentioned WZ ♀-complete male Oreochromis aureus of ZZ ♂ type; The described processing of feminizing is to handle 15 day or more with the pharmaceutical chemistry that contains female sex hormone (FH) the undifferentiated Oreochromis aureus seedling of sexual gland; Carry out normal feeding and management then, can obtain physiology raun (AAZZ △ ♀); The dosage of female sex hormone in the pharmaceutical chemistry (FH) is that every gram feed contains female sex hormone 100～200 micrograms; Water temperature when pharmaceutical chemistry is handled is 25～30 ℃.
In the producing method for seed of above-mentioned WZ ♀-complete male Oreochromis aureus of ZZ ♂ type, described female sex hormone is estradiol.
The present invention compared with prior art has following advantage:
(1) the full milter of heredity cultivated of the inventive method to have a growth fast, individual big; The current processing with androgenic hormone of instead induces so-called full milter to carry out the marketable fish breeding production; Therefore exempting the androgenic hormone processing influences consumer health and ecotope, guarantees that marketable fish is a pollution-free food.
(2) overcoming in the past male and female raises together with and causes the drawback that marketable fish size is uneven, commodity value is low.
The mating filial generation of (3) two kinds of fishes shows as 100% male fish, avoids unnecessary breeding in the marketable fish breed.
(4) select the Oreochromis aureus of two kinds of different strains of the same race to breed, do not relate to other kind of fish, management and easy and simple to handle, and also higher than fertilization rate and the emergence rate of hybrid; The parent and the filial generation mixed questions that also do not exist hybridization to bring.
Description of drawings
Fig. 1 is the process chart of the specific embodiment of the invention.
Embodiment
Below in conjunction with embodiment the present invention is done to specify further, but do not constitute any restriction of the present invention.
Embodiment 1
Adopt the breeding (o.aureus) of the in store two kinds of Oreochromis aureus strains of the national aquatic products seed multiplication farm of our company, the one, introduce from Israel by Taiwan, we are referred to as Ao Liya A strain, and its sex genotype is represented with AAWZ ♀/AAZZ ♂.Another is that introduce from the U.S. in fresh water fishery research center, the Yangtze river basin, is called Ao Liya B strain, and its sex genotype is represented with BBWZ ♀/BBZZ ♂.
Our company is to these two kinds of original seeds, carries out a water (advance, drainage ditch), land (pool, dike, road), empty (bird prevention net) three-dimensional strict isolation protecting kind of field boundary, and carries out seed selection kind, purification and rejuvenation by the standard of biological character and property ratio year after year.Rationally preserve the Ao Liya original seed in these two sources, all valuable for enriching the germplasm gene pool, improve the breed, prevent the degeneration of inbreeding germplasm and making full use of hybrid vigour.
The present invention selects for use the male fry of Ao Liya A strain after feminizing, to be transformed into physiology raun (AAZZ Δ ♀); The concrete optimum organization technology of feminizing; In other a one Chinese patent application file " method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote ", detailed introduction has been arranged, the full content in this article is all included the application's open scope in.Milter with this physiology raun and Ao Liya B strain carries out mating then, produces the full milter of heredity, and its processing technology routine is as shown in Figure 1.
Adopt above-mentioned approach to produce the full milter of Ao Liya (ABZZ ♂) and be the breeding production service, the problem that can effectively avoid inbreeding, germplasm to degenerate makes kind more stable.
The full milter of cultivating by the inventive method of Ao Liya heredity has following characteristics:
(1) male ratio is high, and 100%, Oreochromis aureus natural population male ratio is about 50%.
(2) emergence rate is high, improves about 10% than single strain male and female fish breeding.
(3) cold resistance is good, and the low temperature threshold value of Tilapia mossambica differs at 6 ℃～12 ℃ because of kind usually, and the full milter of this Leah heredity difficult to understand is 4 ℃～6 ℃.
(4) growth is fast, and is faster than Oreochromis aureus nature both sexes population.
(5) adult fish neat specification, marketable fish is worth high.Owing to be the full milter of heredity, do not exist in the process of forming because of male and female individual growth difference and breeding activity, cause that marketable fish size differs, a series of shortcomings of degradation under the output, therefore, the full milter of this Leah difficult to understand has fine market prospect.
(6) the most outstanding characteristics are hereditary male fishes of 100%, and no androgenic hormone pollution problem is the basic assurance of pollution-free food.
Claims (2)
1. the producing method for seed of WZ ♀-complete male Oreochromis aureus of ZZ ♂ type; It is characterized in that; Select for use a kind of male fry of Leah strain difficult to understand after feminizing, to be transformed into the physiology raun, the milter with this physiology raun and another kind of Ao Liya strain carries out mating then, produces the full milter of heredity; Described feminizing is that the undifferentiated Oreochromis aureus seedling of sexual gland is handled more than 15 days with the pharmaceutical chemistry that contains female sex hormone, carries out normal feeding and management then, can obtain the physiology raun; The dosage of female sex hormone is that every gram feed contains female sex hormone 100～200 micrograms in the pharmaceutical chemistry; Water temperature when pharmaceutical chemistry is handled is 25～30 ℃; Described female sex hormone is estradiol.
2. the producing method for seed of WZ ♀ according to claim 1-complete male Oreochromis aureus of ZZ ♂ type; It is characterized in that; Described Leah strain difficult to understand is a kind of to be the strain of being introduced from Israel by Taiwan, and another is the strain that introduce from the U.S. in fresh water fishery research center, the Yangtze river basin.
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| CN200810219397A CN101449664B (en) | 2008-11-25 | 2008-11-25 | Seed production method of WZ(male)-ZZ(female) all-male blue tilapia |
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| CN200810219397A CN101449664B (en) | 2008-11-25 | 2008-11-25 | Seed production method of WZ(male)-ZZ(female) all-male blue tilapia |
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| CN101449664B true CN101449664B (en) | 2012-10-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338104A (en) * | 2018-04-03 | 2019-10-18 | 嘉丰海洋国际股份有限公司 | The method of Oreochromis aureus feminized is induced using the immersion treatment of ethinylestradiol |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101575930B1 (en) * | 2013-06-15 | 2015-12-09 | 구골홀딩스 주식회사 | Method for inducing artificial ovulation and spawn of eel |
| CN108244000A (en) * | 2018-02-08 | 2018-07-06 | 宁波市海洋与渔业研究院 | The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized |
| CN108377939B (en) * | 2018-03-13 | 2021-08-06 | 广西壮族自治区水产科学研究院 | Method for producing hybrid Oreochromis aureus |
| CN110338105A (en) * | 2018-04-03 | 2019-10-18 | 嘉丰海洋国际股份有限公司 | The method for inducing the masculine of bolti using the immersion treatment of Jia Ji Testis ketone |
-
2008
- 2008-11-25 CN CN200810219397A patent/CN101449664B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| C.Melard.Production of a high percentage of male offspring with 17alpha-ethynylestradiol sex-reversed Oreochromis aureus. I. Estrogen sex-reversal and production or F2 pseudofemales.《Aquaculture》.1995,第130卷(第1期),25-34. * |
| 周一红等.性逆转技术在国外水产养殖中的应用.《现代渔业信息》.1993,第8卷(第11期),16-20. * |
| 杨景峰等.罗非鱼全雄控制技术研究进展.《内蒙古民族大学学报(自然科学版)》.2003,第18卷(第4期),318-322. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338104A (en) * | 2018-04-03 | 2019-10-18 | 嘉丰海洋国际股份有限公司 | The method of Oreochromis aureus feminized is induced using the immersion treatment of ethinylestradiol |
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Owner name: XIAMEN LUYE FISHERIES CO., LTD. Free format text: FORMER OWNER: GUANGZHOU LUYE AQUATIC PRODUCTS CO., LTD. Effective date: 20121218 |
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Effective date of registration: 20160226 Address after: Changtai County eco-tourism areas of mayangxi mountain village 363900 Fujian city of Zhangzhou Province Patentee after: Changtai Shengyuan Bacera Aquatic Seed Co. Ltd. Address before: Jimei Zhongzhou town of Jimei District of Xiamen City, Fujian province 361000 Patentee before: Xiamen Heron aquatic products Co., Ltd. |