CN102283149B - Culturing method for sex reversal of female carp to male carp - Google Patents
Culturing method for sex reversal of female carp to male carp Download PDFInfo
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- CN102283149B CN102283149B CN201110186519XA CN201110186519A CN102283149B CN 102283149 B CN102283149 B CN 102283149B CN 201110186519X A CN201110186519X A CN 201110186519XA CN 201110186519 A CN201110186519 A CN 201110186519A CN 102283149 B CN102283149 B CN 102283149B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a culturing method for sex reversal of a female carp to a male carp. The culturing method comprises the following steps that A. squeezing an abdomen of a 10-11-month-old carp, and separating a male carp with out-flown semen and a carp with no out-flown semen; B. inserting a copper roe digging device in a genital pore of the carp from which semen can not be squeezed, and selecting the carp capable of obtaining roe particles to cultivate the carp in a cement pond; C. according to the weight of the carp, feeding the carp with dosing fodders containing 17 alpha- methyltestosterone every day, and mix-injecting domperidone, luteinizing hormone releasing hormone and carp pituitary gland in the thoracic cavity of the carp in each five days; and D. after continuously processing the test carp for 2 months, squeezing the abdomen of the carp, the test carp with out-flown milky white semen is the sex reversal carp which is a female on genotype and a male on physiology. The culturing method is easy and feasible and is convenient in operation, and can be used for sex reversing the female carp on genotype into a male carp on physiology. The growth speed of the filial generation cross bred by the sex reversal carp and the common carp is fast and the economic benefits are remarkable.
Description
Technical field
The present invention relates to aquatic livestock genetic breeding technical field; More specifically relating to a kind of is the breeding method of male carp by female reverse with carp; The genotype that adopts this method to cultivate is female but can be used as the parent for male carp on the physiology; With the seminal fluid of its generation and the ovum hybridization of common female carp generation, just can produce the offspring all is that female carp is applied to aquaculture.
Background technology
Fish are one of main sources of protein food, and the animal protein consumption of Chinese people folk law 1/3 is from aquatic products such as fish, and wherein about 70% aquatic products derive from aquaculture.China is the country that unique in the world aquaculture output surpasses amount of fishing.Improved seeds are bases of the sustainable sound development of aquaculture, and the cultured fishes new varieties of cultivating merit have very important significance.
Carp is one of dominance kind of China's freshwater aquiculture, and according to Ministry of Agriculture's statistics, China's carp culture gross yield in 2008 surpasses 2,350,000 tons, accounts for 11.4% of freshwater aquiculture gross yield.Find that in production practices carp has female than the obvious fast characteristics of male growth rate, 2 ages, female carp was than the average weight high approximately 30% of male carp of the same age; Therefore; Culturing complete female carp will shorten the culture-cycle, practice thrift cost, have tangible economic benefit.
The Sex determination mode of carp is female homotype (XX), diandry (XY).Adopt gynogenesis can directly cultivate complete female carp, but the gynogenesis complicated operation, and production efficiency is lower, can't satisfy extensive aquaculture demand.The gynogenesis technology being combined with the sex reversal technology, obtain female carp through the gynogenesis technology earlier, is the physiological male carp with the female individuals sex reversal again; In case obtain a spot of physiological male carp, then can be through sexual hybridization large-scale production all female carps fish (Wu C, Chen R; Ye Y, Huang W, Production of all-female carp and its application in fish cultivation. Aquaculture; 1990,85:327).
The carp that is physiological male with female carp sex reversal is the key of cultivating all female carps fish on a large scale.Fish belong to the low vertebrate of waiting; Sex hormone plays an important role in the fish sex atomization; Under the effect of exogenous hormone; Can change the original Sex Differentiation direction of fish, making genotype is that the individuality of female (male) is transformed into physiological male (female), thereby sex is taken a turn for the worse.It is generally acknowledged that should promptly carry out in the variable period of sexual gland differentiation direction before the differentiation of sexual gland form hormone-treated initial period, after the fish sex differentiation has been accomplished, carries out HORMONE TREATMENT again, the difficulty that sex reversal takes place fish is very big.Because the carp juvenile fish stage can't be distinguished male and female; Therefore before carrying out sex reversal, need to obtain female young carp through gynogenesis earlier, and then the female young carp of adopting androgenic hormone to handle about 20 day age carries out the sex reversal (Wu Qingjiang of carp; Ye Yuzhen; Chen Rongde, virgin gold is careless, the generation and the biological property thereof of red carp 8305 pure lines.Oceanologia et Limnologia Sinica, 1991,22 (4): 295-299), remain female (XX) thereby obtain sex genotype, but have the spermary of normal development on the phenotype, and can produce the physiological male carp of sperm with normal function.
Summary of the invention
The objective of the invention is to be to provide a kind of breeding method that is male carp with female carp sex reversal; Easy to implement the method; Easy and simple to handle, need not carry out the operation of complex manual gynogenesis, can be that female carp sex reversal is the carp of physiological male directly with genotype.In case the physiological male carp of acquired reverse just can be used as the parent, with the seminal fluid of its generation and the ovum hybridization of common carp generation, thereby the large-scale production filial generation all is female carp.The growth rate of female carp is cultured complete female carp and will be shortened the culture-cycle than the fast growth of male carp, practices thrift cost, has tangible economic benefit.
In order to reach above-mentioned purpose, the present invention adopts following technical measures:
The breeding method that a kind of female carp sex reversal is male carp the steps include:
A, the carp seedling after initial feeding 1-3 days was cultured 10-11 individual month by conventional method in the pond; (the safe and efficient healthy aquaculture pattern of the Yellow River carp, science is breeded fish, and 2010,11:42)
B, 10-11 monthly age carp is taken out from the pond; Support at outdoor cement pit (among the length * wide * depth of water=3.5m * 2.3m * 1.5m) temporarily; Push the carp belly one by one gently, will have seminal fluid male carp of flowing out and the carp that does not have seminal fluid to flow out and separate;
C, the copper egg apparatus that digs (is dug the long 10cm of egg apparatus handle; Front end has long 1.5cm, the groove of wide 0.3cm) insert gently and can not extrude in the carp gonopore of seminal fluid, be partial to left side or right side then; Rotation 3-5 circle is extracted out; If can obtain a small amount of ovum grain, just this raun is picked out, as the object of sex reversal processing;
D, the preparation that will pick out are used for the carp of sex reversal processing at the outdoor cement pit (length * wide * depth of water=3.5m * 2.3m * 1.5m) begin experiment after 1 week of breed.Cultivation density is that every saliva mud sump is no more than 20 fishes;
E, every mornings 8 point, 12 noon and afternoons 4 point; Throw something and feed according to the 5%-7% of carp body weight and to contain 17 Alpha-Methyl testosterones (17 α-Methyltestosterone, MT) carp feed of 50ugMT/g (the honest Co., Ltd in Wuhan product) for three times.Simultaneously; Respectively according to every carp body weight; Whenever at a distance from 5 days in the carp thoracic cavity hybrid injection DOM (Domperidone; DOM) 2mg/kg and Luteinizing hormone releasing-hormone A2 (Luteinizing Hormone Releasing Hormone A2, LHRH-A2) 5 μ g/kg, and carp hypophysis 2mg/kg;
F, the continuous Processing Test carp of E is pushed the carp belly after 2 months gently set by step, have test fish that the milky seminal fluid flows out be exactly genotype be female, and be male sex reversal carp on the physiology;
G, hybridize with the carp of this sex reversal seminal fluid that produces and the ovum that common female carp produces, just can produce the offspring in a large number all is that female carp is used for aquaculture.
The present invention compared with prior art has the following advantages and effect:
Adopt this method need not carry out the operation of complex manual gynogenesis; Can be that female carp sex reversal is the carp of physiological male directly with genotype; And can be with the carp of sex reversal directly as the parent; Through hybridizing with common female carp, the large-scale production filial generation is female carp all, is applied to aquaculture practices.To shorten the culture-cycle because the growth rate of female carp, is therefore cultured complete female carp than the fast growth of male carp, practice thrift cost, have tangible economic benefit.
Embodiment
Embodiment 1:
The breeding method that a kind of female carp sex reversal is male carp the steps include:
A, the carp seedling after initial feeding 1-3 days was cultured 10-11 individual month by conventional method in the pond; (the safe and efficient healthy aquaculture pattern of the Yellow River carp, science is breeded fish, and 2010,11:42)
B, 10-11 monthly age carp is taken out from the pond; Support at outdoor cement pit (among the length * wide * depth of water=3.5m * 2.3m * 1.5m) temporarily; Push the carp belly one by one gently, will have seminal fluid male carp of flowing out and the carp that does not have seminal fluid to flow out and separate;
C, the copper egg apparatus that digs (is dug the long 10cm of egg apparatus handle; Front end has long 1.5cm, the groove of wide 0.3cm) insert gently and can not extrude in the carp gonopore of seminal fluid, be partial to left side or right side then; Rotation 3-5 circle is extracted out; If can obtain a small amount of ovum grain, just this raun is picked out, as the object of sex reversal processing;
D, the preparation that will pick out are used for the carp culture of sex reversal processing at the outdoor cement pit (length * wide * depth of water=3.5m * 2.3m * 1.5m) begin experiment after 1 week.Cultivation density is that every saliva mud sump is no more than 20 fishes;
E, every mornings 8 point, 12 noon and afternoons 4 point; Throw something and feed according to the 5%-7% of carp body weight and to contain 17 Alpha-Methyl testosterones (17 α-Methyltestosterone, MT) carp feed of 50ugMT/g (the honest Co., Ltd in Wuhan product) for three times.Simultaneously; Respectively according to every carp body weight; Whenever at a distance from 5 days in the carp thoracic cavity hybrid injection DOM (Domperidone; DOM) 2mg/kg and Luteinizing hormone releasing-hormone A2 (Luteinizing Hormone Releasing Hormone A2, LHRH-A2) 5 μ g/kg, and carp hypophysis 2mg/kg;
F, the continuous Processing Test carp of E is pushed the carp belly after 2 months gently set by step, have test fish that the milky seminal fluid flows out be exactly genotype be female, and be male sex reversal carp on the physiology.
Embodiment 2:
The carp of this sex reversal all is the application in the female carp cultivating filial generation: its process is: (Wang Xue outstanding person Hu Yinchang tries to gain Xi Dongluo and builds benevolence according to conventional method common female carp to be carried out artificial induced spawning; Breeding of high-quality brocade carp and cultivation technology; Aquatic science and technology information, 2008,35 (5) 241-244.); The ovum that common carp is produced is hybridized with the seminal fluid that the carp of sex reversal produces, and just can produce the offspring in a large number all is that female carp is used for aquaculture.
Claims (1)
1. the breeding method that female carp sex reversal is male carp the steps include:
A, the carp seedling after initial feeding 1-3 days was cultured 10-11 individual month by conventional method in the pond;
B, 10-11 monthly age carp is taken out from the pond, support temporarily in outdoor cement pit, push the carp belly one by one, seminal fluid male carp of flowing out and the carp that does not have the seminal fluid outflow are separated;
C, the copper egg apparatus that digs is inserted and can not extrude in the carp gonopore of seminal fluid, deflection left side or right side then, rotation 3-5 circle is extracted out, obtains the ovum grain, just this raun is picked out, be the object of sex reversal processing;
D, the preparation that will pick out are used for the carp culture of sex reversal processing at outdoor cement pit, and cultivation density is that every saliva mud sump is no more than 20 fishes;
E, every mornings 8 point, 12 noon and afternoons 4 point; Throw something and feed according to the 5%-7% of carp body weight and to contain the carp feed of 17 Alpha-Methyl testosterone 50ug/g for three times; Simultaneously; Respectively according to every carp body weight, whenever at a distance from 5 days in the carp thoracic cavity hybrid injection DOM 2mg/kg and Luteinizing hormone releasing-hormone A2 5 μ g/kg, and carp hypophysis 2mg/kg;
F, the continuous Processing Test carp of E after 2 months set by step, extruding carp belly, have test fish that the milky seminal fluid flows out be exactly genotype be female, and be male sex reversal carp on the physiology.
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| CN201110186519XA CN102283149B (en) | 2011-07-05 | 2011-07-05 | Culturing method for sex reversal of female carp to male carp |
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Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103070120B (en) * | 2013-01-29 | 2015-08-05 | 水利部中国科学院水工程生态研究所 | Set up the method for complete female yellow cartfish breeding population |
| CN104285863B (en) * | 2014-10-21 | 2016-08-24 | 浙江省海洋水产研究所 | A kind of method inducing gynogenesis Nibea albiflora puppet milter |
| CN106069948A (en) * | 2016-07-15 | 2016-11-09 | 广东海洋大学 | A kind of method improving betta splendens male ratio |
| CN108377936B (en) * | 2017-03-24 | 2020-11-24 | 佛山市南海百容水产良种有限公司 | Large-scale production method of all-female mandarin fish |
| CN108184754B (en) * | 2017-04-17 | 2021-01-01 | 中国水产科学研究院长江水产研究所 | Method for inducing male giant salamander in China at high temperature |
| CN107079844B (en) * | 2017-05-08 | 2018-02-13 | 中国科学院水生生物研究所 | A kind of supermale carp and Genetic male and the preparation method of physiology female carp |
| CN109924149B (en) * | 2019-04-22 | 2021-05-28 | 宁德师范学院 | Method for promoting conversion of epinephelus akaara parents to males |
| CN112544516A (en) * | 2020-12-08 | 2021-03-26 | 汕头大学 | Artificial reversing method for scylla paramamosain |
| CN115500286A (en) * | 2022-09-19 | 2022-12-23 | 中山大学 | Method for obtaining pseudo-female siniperca chuatsi through artificial induction |
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| JPH10150883A (en) * | 1996-11-21 | 1998-06-09 | Kanagawa Pref Gov | Method for creating total male triploid fish by using sex reversal male fish of ayu |
| CN101120658A (en) * | 2006-08-08 | 2008-02-13 | 白宝海 | All female method for salmon and bulltrout |
| CN101390503B (en) * | 2008-11-03 | 2011-09-14 | 水利部中国科学院水工程生态研究所 | Method for establishing super-male yellow cartfish breeding population by copulating super-male fish with physiology spawner |
| CN101416613A (en) * | 2008-11-25 | 2009-04-29 | 广州鹭业水产有限公司 | The method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote |
| JP5390484B2 (en) * | 2009-07-28 | 2014-01-15 | 中部飼料株式会社 | Puffer sex change method, blowfish breeding method, and sex change blowfish obtained by the method |
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