CN108377939B - Method for producing hybrid Oreochromis aureus - Google Patents
Method for producing hybrid Oreochromis aureus Download PDFInfo
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- CN108377939B CN108377939B CN201810206109.9A CN201810206109A CN108377939B CN 108377939 B CN108377939 B CN 108377939B CN 201810206109 A CN201810206109 A CN 201810206109A CN 108377939 B CN108377939 B CN 108377939B
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 241000219233 Oreochromis aureus Species 0.000 title claims description 6
- 241000276707 Tilapia Species 0.000 claims abstract description 107
- 238000000034 method Methods 0.000 claims abstract description 30
- 241000276703 Oreochromis niloticus Species 0.000 claims abstract description 28
- 238000009395 breeding Methods 0.000 claims abstract description 28
- 230000001488 breeding effect Effects 0.000 claims abstract description 28
- 241000276701 Oreochromis mossambicus Species 0.000 claims abstract description 22
- 210000003765 sex chromosome Anatomy 0.000 claims abstract description 16
- 235000013601 eggs Nutrition 0.000 claims description 18
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 16
- 241000251468 Actinopterygii Species 0.000 claims description 15
- 241000276719 Oreochromis Species 0.000 claims description 13
- GCKMFJBGXUYNAG-UHFFFAOYSA-N 17alpha-methyltestosterone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C)(O)C1(C)CC2 GCKMFJBGXUYNAG-UHFFFAOYSA-N 0.000 claims description 10
- GCKMFJBGXUYNAG-HLXURNFRSA-N Methyltestosterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@](C)(O)[C@@]1(C)CC2 GCKMFJBGXUYNAG-HLXURNFRSA-N 0.000 claims description 10
- 229960001566 methyltestosterone Drugs 0.000 claims description 10
- 230000001568 sexual effect Effects 0.000 claims description 10
- 230000012447 hatching Effects 0.000 claims description 9
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 8
- 239000005711 Benzoic acid Substances 0.000 claims description 8
- 235000010233 benzoic acid Nutrition 0.000 claims description 8
- 229960005309 estradiol Drugs 0.000 claims description 8
- 229930182833 estradiol Natural products 0.000 claims description 8
- UYIFTLBWAOGQBI-BZDYCCQFSA-N Benzhormovarine Chemical compound C([C@@H]1[C@@H](C2=CC=3)CC[C@]4([C@H]1CC[C@@H]4O)C)CC2=CC=3OC(=O)C1=CC=CC=C1 UYIFTLBWAOGQBI-BZDYCCQFSA-N 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 229950002007 estradiol benzoate Drugs 0.000 claims description 3
- 238000011161 development Methods 0.000 abstract description 5
- 238000003975 animal breeding Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000008014 freezing Effects 0.000 description 4
- 238000007710 freezing Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000026109 gonad development Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 244000180534 Berberis hybrid Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for producing hybrid tilapia in Orgi, belonging to the technical field of aquatic animal breeding. The seed production method comprises the following steps: A. breeding pseudo-female Oreochromis mossambica with ZZ sex chromosome and female sex chromosome XX and male pseudo-male gift tilapia with male sex chromosome until the sex is mature; B. and D, hybridizing the pseudo-female oreochromis niloticus obtained in the step A as a female parent and the pseudo-male gift tilapia as a male parent to obtain the hologynic oreochromis niloticus fingerling. The fingerling obtained by the method has higher male rate and stress resistance than that of common gift tilapia, has higher emergence amount and growth speed than that of common Aoni tilapia, meets the development requirement of the industry on tilapia, and has extremely high market value.
Description
Technical Field
The invention relates to a method for producing seeds of tilapia, in particular to a method for producing seeds of hybrid tilapia in Orgji, belonging to the technical field of aquatic animal breeding.
Background
Tilapia (Oreochromys) is a warm water fish native to tropical and subtropical regions, and is an international fish and trade aquatic product intensively popularized and cultured by the Food and Agriculture Organization (FAO) of the United nations. The history of tilapia culture in China is not long, but the tilapia culture develops very rapidly, the tilapia yield increases by about 10% every year in the early stage of twenty-first century, the increase rate is slowed down in recent years, but the tilapia culture, processing and export quantity in China are still on the first place in the world.
Currently, the main species of tilapia cultivated in China are gift tilapia and ornithoctopus. The gift tilapia is the tilapia variety which grows the fastest, and the tilapia grows at a slower speed than the gift tilapia, but has stronger stress resistance. Since 2008, streptococcicosis and freezing disaster of tilapia have great impact on the tilapia industry, great loss is caused every year, the most serious tilapia suffering from the streptococcicosis and freezing disaster is gift tilapia in front of the diseases and the freezing disaster, and the tilapia withstands the tests of the diseases and the freezing disaster due to the strong disease and cold resistance.
In recent years, the sale price and the sale quantity of the tilapia fries are steadily increased, and even the tilapia is difficult to obtain one fry. Although the tilapia has the advantages of disease resistance and cold resistance, the defects are not ignored, and the main expression is the following 4 points: (2) the growth speed of the Ornity tilapia is obviously slower than that of the gift tilapia, and the culture time of the Ornity tilapia reaches the market specification and is more than 1 month more than that of the gift tilapia; (2) the fry yield is low, the Ornithoia mossambica is an interspecific hybrid variety, the hybrid reproductive capacity is far inferior to intraspecific mating, the average fry yield is only about 1/3 of the Tilapia gilvus, and the seed production cost is high; (3) the male rate is unstable, the male rate of the Ornity hybrid tilapia is 100% theoretically, the male rate can generally reach more than 90% in actual production, and the male rate is only about 70% and is difficult to meet the production requirement.
In order to solve the problems of the tilapia, parent breeding and large-scale seed production technical research of the tilapia is carried out in simplified Wei industry, plum blossom and the like, the growth speed of the tilapia bred in 2009 is higher than that of the tilapia, and the seedling emergence amount of the tilapia bred by breeding is only 1300 fish/year/tail female fish because the group breeding has an unsatisfactory effect on reproductive performance selection. Due to high breeding and breeding costs of the tilapia mossambica (including the gift tilapia mossambica) and low fry yield, a strange image of 'lack price of the fry' is caused, and on one hand, the fry field produces less fries and is difficult to profit, and on the other hand, farmers are difficult to grow the fries and increase the cost.
Therefore, the 'fast growth and fast death' of the gift tilapia and the 'slow growth and difficult seedling demand' of the Ornity tilapia are difficult to adapt to the development requirements of the tilapia industry, the development of the current tilapia industry needs a new generation of tilapia variety to drive, and the variety can not grow too slowly or die too fast and has enough seedling supply.
Disclosure of Invention
The invention aims to solve the technical problems that in the prior art, the gift tilapia grows fast and dies fast, the tilapia ornithogamicus grows slow and is difficult to obtain seedlings, and the development requirements of the current tilapia industry cannot be met.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a method for producing hybrid Oregai tilapia seeds is characterized in that: the method comprises the following steps:
A. breeding pseudo-female Oreochromis mossambica with ZZ sex chromosome and female sex chromosome XX and male pseudo-male gift tilapia with male sex chromosome until the sex is mature;
B. and D, hybridizing the pseudo-female oreochromis niloticus obtained in the step A as a female parent and the pseudo-male gift tilapia as a male parent to obtain the hologynic oreochromis niloticus fingerling.
Further, in step a of the present invention, the method for breeding pseudo-female oreochromis niloticus is as follows:
a1. collecting fertilized eggs of oreochromis niloticus, incubating by adopting an artificial incubation method, soaking the fertilized eggs by estradiol containing benzoic acid after rupture of membranes, incubating to obtain fry, and continuously feeding feed containing estradiol containing benzoic acid after the fry is obtained by opening the mouth to obtain the all-female oreochromis niloticus;
a2. taking the hologynic oreochromis niloticus obtained by the cultivation in the step a1 as a female parent and taking the common male oreochromis niloticus as a male parent, and pairing in a net cage to obtain fries of a plurality of families;
a3. and c, selecting fry from each family in the step a2, feeding the fry with common feed, breeding the fry to 20g, selecting the tilapia family with a full male phenotype, treating the fry of the same family by the method in the step a1 to obtain a pseudo female oreochromis tilapia family, and breeding the pseudo female oreochromis tilapia family to sexual maturity.
Furthermore, in the step a1 of the invention, the content of estradiol benzoate in the feed is 300 mg/kg.
Further, in the step a1, the feeding method of the feed is 3-4 times a day and the feeding time is 30 days.
Further, in step a2 of the present invention, the ratio of male to female is 2:1 when mated.
Further, in the step a2 of the present invention, after pairing for 10 days, fry were collected.
Further, in the step A of the invention, the method for cultivating the full-male gift tilapia comprises the following steps:
a11. collecting fertilized eggs of the gift tilapia, hatching by adopting an artificial hatching method, and after fish fries are obtained, continuously feeding feed containing methyltestosterone after the fish fries are fed in an opening manner to obtain the all-male gift tilapia;
a22. pairing the fully male gift tilapia bred in the step a11 as a male parent and a common female as a female parent in a net cage to obtain fries of a plurality of families;
a33. and c, selecting fry from each family in the step a22, feeding the fry with common feed, breeding the fry to 20g, selecting the family of the gift tilapia with the full female phenotype, treating the fry of the same family by the method in the step a11 to obtain pseudo-male gift tilapia, and breeding the fry to sexual maturity.
Furthermore, in the step a11 of the invention, the content of methyltestosterone in the feed is 40 mg/kg.
Further, in the step a11, the feeding method of the feed is 3-4 times a day and the feeding time is 30 days.
Further, in step a22 of the present invention, the ratio of male to female is 2:1 when mated.
The invention has the beneficial effects that:
the invention obtains a new male gift tilapia variety through the pseudo-female gift tilapia (the sex chromosome is ZZ, the phenotype is female) and the pseudo-male gift tilapia (the sex chromosome is XX, the phenotype is male), the male gift tilapia variety has a offspring male rate and stress resistance higher than those of the common gift tilapia variety, the emergence amount and the growth speed are higher than those of the common tilapia variety, the development requirements of the industry on the tilapia are met, and the invention has extremely high market value.
Detailed Description
The technical scheme of the invention is further described by combining specific examples, wherein the experimental method without specific conditions noted in the examples is generally carried out according to conventional conditions, and the technology which is not described in detail is conventional technology. All experiments in the following examples were performed in the national-grade south-China tilapia stock farm.
Example 1
A method for producing hybrid Oregai tilapia seeds is characterized in that: the method comprises the following steps:
A. breeding pseudo-female Oreochromis mossambica with ZZ sex chromosome and female sex chromosome XX and male pseudo-male gift tilapia with male sex chromosome until the sex is mature;
B. and D, hybridizing the pseudo-female oreochromis niloticus obtained in the step A as a female parent and the pseudo-male gift tilapia as a male parent to obtain the hologynic oreochromis niloticus fingerling.
In step a of this example, the method for breeding the pseudo-female oreochromis niloticus is as follows:
a1. collecting fertilized eggs of oreochromis niloticus, incubating by adopting an artificial incubation method, soaking the fertilized eggs by estradiol containing benzoic acid after rupture of membranes, incubating to obtain fry, and continuously feeding feed containing estradiol containing benzoic acid after the fry is obtained by opening the mouth to obtain the all-female oreochromis niloticus;
a2. taking the hologynic oreochromis niloticus obtained by the cultivation in the step a1 as a female parent and taking the common male oreochromis niloticus as a male parent, and pairing in a net cage to obtain fries of a plurality of families;
a3. and c, selecting fry from each family in the step a2, feeding the fry with common feed, breeding the fry to 20g, selecting the tilapia family with a full male phenotype, treating the fry of the same family by the method in the step a1 to obtain a pseudo female oreochromis tilapia family, and breeding the pseudo female oreochromis tilapia family to sexual maturity.
In step a1 of this example, the content of estradiol benzoate in the feed was 300 mg/kg.
In step a1 of this embodiment, the feeding method of the feed is 3-4 times a day, and the feeding is performed for 30 days.
In step a2 of this example, the ratio of male to female was 2:1 at the time of pairing.
In step a2 of this example, fry were collected 10 days after pairing.
In step a of this example, the method for breeding the fully male gift tilapia is as follows:
a11. collecting fertilized eggs of the gift tilapia, hatching by adopting an artificial hatching method, and after fish fries are obtained, continuously feeding feed containing methyltestosterone after the fish fries are fed in an opening manner to obtain the all-male gift tilapia;
a22. pairing the fully male gift tilapia bred in the step a11 as a male parent and a common female as a female parent in a net cage to obtain fries of a plurality of families;
a33. and c, selecting fries from each family in the step a22 to feed common feed, breeding to 20g, selecting a gift tilapia family with a full female phenotype, treating the fries of the same family by the method in the step a11, namely collecting fertilized eggs, carrying out artificial incubation, feeding feed containing methyltestosterone continuously after opening feeding to obtain pseudo-male gift tilapia, and breeding to sexual maturity.
In step a11 of this example, the content of methyltestosterone in the feed was 40 mg/kg.
In step a11 of this embodiment, the feeding method of the feed is 3-4 times a day, and the feeding is performed for 30 days.
In step a22 of this example, the ratio of male to female was 2:1 at the time of pairing.
Example 2
A method for producing hybrid Oreochromis aureus, which comprises the following steps:
A1. cultivation of pseudo-female Oreochromis mossambica (sex chromosome ZZ, phenotype female)
(1) Collecting fertilized eggs of oreochromis niloticus, incubating by an artificial incubation method, soaking the fertilized eggs after membrane rupture by estradiol containing benzoic acid, incubating to obtain seedlings, randomly extracting 300 fries, putting the fries into 3 water tanks of 45cm multiplied by 75cm multiplied by 20cm (width multiplied by length multiplied by height), feeding the fries in each water tank with 100 tails, continuously feeding estradiol containing benzoic acid feed with the content of 300mg/kg of bulk feed for 30 days after the fries are opened, transferring the tilapia fries in 3 water tanks into a 20-mesh net cage of 2m multiplied by 3m multiplied by 1.5m (width multiplied by length multiplied by height) to about 20g after the drug treatment is completed, counting the female-male ratio of the oreochromis nilotis, finding that the female rate of 3 family tilapia is 100%, and randomly extracting 30 fries from each net cage to reach sexual maturity;
(2) randomly extracting 20 tails of Oreochromis mossambicus with good gonad development phenotype from the net cage as female parents, taking 10 tails of common male Oreochromis mossambicus as male parents, pairing in a net cage with 20 meshes of 10 1.5m multiplied by 1.5m (width multiplied by length multiplied by height) according to the nest pairing method, naturally mating and fertilizing the Tilapia mossambicus in the net cage, collecting fertilized eggs and artificially incubating to obtain 11 family fries after pairing for 10 days;
(3) selecting 100 fish fries at random for each family, feeding a feed containing benzoic acid estradiol continuously when the fish fries are open to eat, feeding a pelletized feed with the content of 300mg/kg for 30 days to obtain phenotype full-female oreochromis niloticus, then randomly selecting 100 fish fries from each family, feeding a common feed as a control group, breeding to about 20g, counting the ratio of female to male, finding that 4 control groups are full-male families, selecting 3 pseudo-female oreochromis niloticus after the 4 families are treated by benzoic acid estradiol, breeding to reach sexual maturity, and obtaining 1350 fish fries of pseudo-female oreochromis nilotis niloticus.
A2. Breeding of pseudo-male gift tilapia (sex chromosome is XX, phenotype is male)
(1) Collecting fertilized eggs of tilapia, hatching by adopting an artificial hatching method to obtain fries, randomly extracting 300 fries, putting the fries into 3 water tanks of 45cm multiplied by 75cm multiplied by 20cm (width multiplied by length multiplied by height) for feeding, wherein 100 fries are arranged in each water tank, the fries are fed after being opened, namely, the fries are continuously fed with a bulk feed with the methyltestosterone content of 40mg/kg for 20 days, after the drug treatment is completed, the tilapia fries in the 3 water tanks are respectively transferred into a net cage of 20 meshes of 2m multiplied by 3m multiplied by 1.5m (width multiplied by length multiplied by height) for feeding to about 20g, counting the male and female ratio of the tilapia, finding that the male rate of 3 net cages is 100 percent, and randomly extracting 20 fries from each net cage for breeding to sexual maturity;
(2) randomly extracting 18 male tails with good gonad development phenotype from the net cage as male parents, taking 36 common female tails as female parents, pairing the male and female parents in 18 net cages with 40 meshes, namely 1.5m multiplied by 1.5m (width multiplied by length multiplied by height) according to a nest pairing method, naturally mating and fertilizing tilapia in the net cages, collecting fertilized eggs after pairing for 10 days, and artificially incubating to obtain 11 family fries;
(3) randomly selecting 100 families from each family to obtain phenotype hologynes according to the method in the step (1), randomly selecting 100 families to feed common feed as a control group, culturing to about 20g, counting the proportion of the males and the females, finding that 6 families of the control group are hologynes, wherein the male rate of the tilapia treated by the methyltestosterone in the 6 families is 100 percent, and selecting and reserving 3 pseudo-male families to culture to sexual maturity;
(4) 3 pseudo-male families are used as male parents, common female fishes are used as female parents, pairing propagation is carried out in a 40-mesh net cage with the size of 3m multiplied by 10m multiplied by 1.5m (width multiplied by length multiplied by height), fertilized eggs are manually collected and incubated, 100 tilapia mossambica are randomly selected from each family according to the method (1) to obtain phenotype full males, the rest tilapia mossambica are cultured by common feed, the ratio of males and females is counted, the tilapia mossambica treated by methyl testosterone is all male, the tilapia mossambica cultured commonly is female, 11.7 ten thousand tilapia mossambica tails with pseudo-male gift are obtained together, and large-scale full-female seed production and batch production of pseudo-male are realized.
B. Hybrid Oreochromis japonica seed production
Screening 150 tails of pseudo-female oreochromis tilapia as female parents and 60 tails of pseudo-male gift tilapia as male parents, carrying out pairing propagation in a 40-mesh net cage with 3 meshes of 3m multiplied by 10m multiplied by 1.5m (width multiplied by length multiplied by height), manually collecting fertilized eggs after 10 days of pairing, and hatching to obtain oreochromis hybrid tilapia fries, wherein common oreochromis tilapia is used as a control group. Collecting fertilized eggs of oreochromis niloticus and incubating to obtain 10.61 ten thousand fries of offspring seeds, wherein 3 net cages respectively comprise 3.67 ten thousand tails, 3.20 ten thousand tails and 3.74 ten thousand tails; obtaining 3.78 ten thousand seeds of Oreochromis niloticus fries, namely 1.26 thousand seeds, 1.03 thousand seeds and 1.49 thousand seeds, wherein the emergence amount of Oreochromis niloticus is remarkably higher than that of common Oreochromis nilotis niloticus;
(2) the main economic performance comparison experiment is carried out by taking common Oreochromis mossambicus as a control group, the culture experiment is carried out in a pond, 2000 tilapia fries are put in each mu averagely, 1000 tilapia mossambicus and 1000 tilapia mossambicus are respectively put in each mu, and fin marking and pond culture are carried out, the result shows that the growth speed and the uniformity of the Oreochromis mossambicus are higher than those of the Oreochromis mossambicus, the male rate reaches 100 percent (the Oreochromis mossambicus is 92.7 percent), and the culture survival rate has no significant difference with the Oreochromis mossambicus.
TABLE 1 comparison table of main economic properties of Oreochromis aureus and Oreochromis aureus
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and all simple modifications and equivalent variations of the above embodiments according to the technical spirit of the present invention are included in the scope of the present invention.
Claims (5)
1. A method for producing hybrid Oregai tilapia seeds is characterized in that: the method comprises the following steps:
A. breeding pseudo-female Oreochromis mossambica with ZZ sex chromosome and female sex chromosome XX and male pseudo-male gift tilapia with male sex chromosome until the sex is mature;
B. taking the pseudo-female oreochromis niloticus obtained in the step A as a female parent and the pseudo-male gift tilapia as a male parent, and hybridizing to obtain hologynic oreochromis niloticus fries;
in the step A, the method for cultivating the pseudo-female Oreochromis aureus is as follows:
a1. collecting fertilized eggs of oreochromis niloticus, incubating by adopting an artificial incubation method, soaking the fertilized eggs by estradiol containing benzoic acid after rupture of membranes, incubating to obtain fry, and continuously feeding feed containing estradiol containing benzoic acid after the fry is obtained by opening the mouth to obtain the all-female oreochromis niloticus;
a2. taking the hologynic oreochromis niloticus obtained by the cultivation in the step a1 as a female parent and taking the common male oreochromis niloticus as a male parent, and pairing in a net cage to obtain fries of a plurality of families;
a3. selecting fry from each family in the step a2, feeding the fry with common feed, breeding the fry to 20g, selecting a tilapia family with a full male phenotype, treating the fry of the same family by the method in the step a1 to obtain a pseudo female oreochromis tilapia family, and breeding the pseudo female oreochromis tilapia family to sexual maturity;
in the step a1, the content of estradiol benzoate in the feed is 300 mg/kg;
in the step a1, the feeding method of the feed is 3-4 times a day and the feeding is carried out for 30 days;
in the step a2, when in matching, the ratio of male to female is 2: 1;
in the step A, the method for cultivating the pseudo-male gift tilapia comprises the following steps:
a11. collecting fertilized eggs of the gift tilapia, hatching by adopting an artificial hatching method, and after fish fries are obtained, continuously feeding feed containing methyltestosterone after the fish fries are fed in an opening manner to obtain the all-male gift tilapia;
a22. pairing the fully male gift tilapia bred in the step a11 as a male parent and a common female as a female parent in a net cage to obtain fries of a plurality of families;
a33. and c, selecting fry from each family in the step a22, feeding the fry with common feed, breeding the fry to 20g, selecting the family of the gift tilapia with the full female phenotype, treating the fry of the same family by the method in the step a11 to obtain pseudo-male gift tilapia, and breeding the fry to sexual maturity.
2. The method for producing hybrid tilapia in Orgi according to claim 1, characterized in that: in step a2, after pairing for 10 days, fry are collected.
3. The method for producing hybrid tilapia in Orgi according to claim 1, characterized in that: in step a11, the content of methyltestosterone in the feed is 40 mg/kg.
4. The method for producing hybrid tilapia in Orgi according to claim 1, characterized in that: in the step a11, the feeding method of the feed is 3-4 times a day and the feeding is carried out for 30 days.
5. The method for producing hybrid tilapia in Orgi according to claim 1, characterized in that: in step a22, the ratio of male to female is 2:1 when mated.
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| CN103583427A (en) * | 2013-11-06 | 2014-02-19 | 广西壮族自治区水产科学研究院 | Pseudo-male and full-female nile tilapia fingerling production method |
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| CN101292633B (en) * | 2007-04-28 | 2011-11-16 | 李思发 | Bredding method for hybridization of fish-Ji'ao tilapia |
| CN101449664B (en) * | 2008-11-25 | 2012-10-10 | 广州鹭业水产有限公司 | Seed production method of WZ(male)-ZZ(female) all-male blue tilapia |
| JP6536961B2 (en) * | 2014-03-26 | 2019-07-03 | 国立大学法人東京海洋大学 | Method for producing fish gametes for cultured fish production using surrogate parent fish, which is applicable to parent species of another genus fish species |
| CN105360034A (en) * | 2015-11-30 | 2016-03-02 | 烟台开发区天源水产有限公司 | Fry preparation method for all-male takifugu rubripus and application |
| CN106818546B (en) * | 2016-12-05 | 2020-04-17 | 广西壮族自治区水产科学研究院 | Breeding method of high-reproduction type hybrid mating mantle line of Niao tilapia |
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| CN103583427A (en) * | 2013-11-06 | 2014-02-19 | 广西壮族自治区水产科学研究院 | Pseudo-male and full-female nile tilapia fingerling production method |
Non-Patent Citations (2)
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| 吉富罗非鱼与奥利亚罗非鱼完全双列杂交后代生长性能与肌肉;强俊等;《中国水产科学》;20150731(第4期);第654-665页 * |
| 苯甲酸雌二醇诱导新吉富罗非鱼雌性化的研究;万松良;《水产学杂志》;20121231;第29-32页 * |
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