CN103583427B - Pseudo-male and complete female bolti producing method for seed - Google Patents
Pseudo-male and complete female bolti producing method for seed Download PDFInfo
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- CN103583427B CN103583427B CN201310546027.6A CN201310546027A CN103583427B CN 103583427 B CN103583427 B CN 103583427B CN 201310546027 A CN201310546027 A CN 201310546027A CN 103583427 B CN103583427 B CN 103583427B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses pseudo-male and complete female bolti producing method for seed.Step is as follows: 1. throw something and feed containing the feed 15 days of Methyltestosterone after bolti opening continuously, obtains the complete male Tilapia mossambica of phenotype and cultivates to sexual maturity; 2. with the complete male Tilapia mossambica of above-mentioned phenotype for male parent, common female bolti is maternal, adopts male and female 1:1 to set up sib families; 3. each family is randomly drawed 100 tail fries and is carried out Methyltestosterone process, get the common raising of 100 tail in addition at random as a control group, the female-male proportion of statistics control group, drug treating group milter corresponding to complete female control group is the male Tilapia mossambica of puppet of sex chromosome X X, and pseudo-male Tilapia mossambica of selecting and remain cultivates sexual maturity; 4. the offspring's sex chromosome utilizing pseudo-male Tilapia mossambica and common Tilapia mossambica to breed is XX, and common cultivation can realize the complete female bolti scale production of hybrid seeds, and Methyltestosterone process can be mass pseudo-male bolti.The inventive method can improve Tilapia mossambica kind fish quality, improves seed production efficiency, reduces breeding cost.
Description
Technical field
The invention belongs to aquatic livestock breeding field.Specifically pseudo-male and complete female bolti producing method for seed.
Background technology
Lucky rich strain bolti (hereinafter referred to as gift tilapia) and Sarotherodon sp are the topmost Tilapia mossambica breed varieties of China, the production female parent of these 2 kind Tilapia mossambica seeds is all female bolti, because Tilapia mossambica fertility is low, plant fish screening conditions harsh, fish needs to drop into a large amount of manpower and financial resources preparation Tilapia mossambica kind, and wherein female Oreochromis niloticus fish preparation is the key factor that impact drops into.
The seed selection of Tilapia mossambica kind fish generally with growth, form, body colour, striped etc. for index, generally screened stage by stage through 3 times.The quantity that gift tilapia expands numerous fry is that plan is selected and remain more than 7 times of kind of fish quantity, and raise about 50g and can carry out first time and screen, female, the milter rate of selecting and remain is respectively 50% and about 20%; Raise and carry out programmed screening to about 200g, female, the milter rate of selecting and remain is respectively 60% and about 50%; Raise about 400g and carry out third time screening, female, the milter rate of selecting and remain is respectively 70% and about 70%; Female, the male rate of selecting and remain of planting fish final of gift tilapia requires 20% and about 7% respectively, amounts to and eliminates quantity more than 85%, eliminate weight more than 60%.Sarotherodon sp is be male parent and bolti with Oreochromis aureus is that female parent is hybridized, and expand numerous bolti and raise about 50g and can carry out first time screening, the raun rate of selecting and remain is respectively 40%, and milter is all eliminated; Raise and carry out programmed screening to about 200g, the raun rate of selecting and remain is 50%; Raise about 400g and carry out third time screening, the raun rate of selecting and remain is 50%; Female, the male rate of selecting and remain of planting fish final of Tilapia mossambica is respectively about 10% and 0%, amounts to superseded quantity and gets over 95%, eliminate weight 80%.Due to 2 ~ 4 times that the maternal production of hybrid seeds quantity of Tilapia mossambica is male parent, early stage male and female difficulty is distinguished, superseded a large amount of Tilapia mossambica is needed in Breeding Process, wherein male Tilipia is main, particularly plant in fish seed selection at Sarotherodon sp female parent, need superseded all male boltis, workload and fund input are all larger.At present, have high input in the maternal production of hybrid seeds of Tilapia mossambica seed multiplication farm ubiquity Tilapia mossambica, take up an area many, that workload is large problem, often become the bottleneck of restriction Tilapia mossambica seed multiplication farm development.
Tilapia mossambica sexual control is the focus of research for a long time, uses Sex Control can improve the controllability of the Tilapia mossambica production of hybrid seeds, realizes planned kind of sashimi (raw fish) and produces, improve seed production efficiency, reduce breeding cost, and the quality of Tilapia mossambica kind fish can be improved, ensure fry quality.
Summary of the invention
The object of the invention is to overcome the deficiency existed in existing bolti seeding technique, a kind of pseudo-male and complete female bolti producing method for seed is provided, Tilapia mossambica kind fish quality can be improved, improve seed production efficiency, reduce breeding cost.
The technical scheme that the present invention solves the problems of the technologies described above is as follows.
Pseudo-male and complete female bolti producing method for seed, producing method for seed step is as follows:
1. collect bolti fertilized egg to carry out artificial incubation and obtain fry, throw something and feed continuously after initial feeding containing the feed 15 days of Methyltestosterone, obtain the complete male bolti of phenotype and cultivate sexual maturity;
2. with the male bolti of phenotype for male parent, common female bolti is maternal, matches 20 groups according to nido matched pair technique male and female quantity 1:1 in net cage, collects fertilized egg artificial incubation and obtains Tilapia mossambica fry family;
3. each family selects the 100 tails feed of throwing something and feeding containing Methyltestosterone to obtain phenotype complete male bolti is at random drug treating group, 100 tails normal diet of throwing something and feeding is selected to be control group at random, cultivate to about 20g, the female-male proportion of statistics control group, drug treating group corresponding to entirely female control group family is that pseudo-male Tilapia mossambica family, phenotype are male, sex chromosome X X, and pseudo-male bolti family of selecting and remain cultivates sexual maturity;
4. the offspring's sex chromosome utilizing pseudo-male bolti and common bolti to breed is XX, and common cultivation can realize the complete female bolti scale production of hybrid seeds, carries out androgenic hormone process can be mass pseudo-male bolti to fry.
The batch production of the male bolti of above-mentioned puppet: the Tilapia mossambica family fry that androgenic hormone process is female parent with the male bolti of puppet for the common female bolti of male parent, obtains phenotype male Tilipia and is pseudo-male bolti.
The above-mentioned complete female bolti scale production of hybrid seeds: with the male bolti family of puppet for male parent, common female bolti is maternal, and the filial generation sex chromosome of breeding is XX type, and common mode cultivation can obtain complete female bolti.
The advantage of the inventive method:
The inventive method can improve Tilapia mossambica kind fish quality, improves seed production efficiency, reduces breeding cost.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
The experimental technique of unreceipted actual conditions in embodiment, usually conveniently condition, unspecified technology is routine techniques.All experiments all complete at national Nanning Tilapia mossambica seed multiplication farm.
Embodiment 1
Pseudo-male as follows with complete female bolti producing method for seed step:
1. collect tilapia oosperm, the method hatching of artificial incubation is adopted to obtain fry, randomly draw 300 tail fries, put into 3 45cm × 75cm × 20cm(wide × long × high) water tank raise, each water tank 100 tail, fry initial feeding Methyltestosterone content of namely throwing something and feeding continuously is the bulk feed 15 days of 40mg/kg, after completing drug treating, respectively the Tilapia mossambica seedling of 3 water tanks is transferred to 2m × 3m × 1.5m(wide × long × high) support to about 20g in 20 order net cages, the female-male proportion of statistics Tilapia mossambica, find that the male ratio of 3 net cage Tilapia mossambicas is 100%, each net cage is randomly drawed 20 tails and is cultivated sexual maturity.
2. from above-mentioned net cage, randomly draw male bolti 20 tail of the good phenotype of gonad development as male parent, common female bolti 20 tail is as female parent, wide × long × high at 20 1.5m × 1.5m × 1.5m(according to nido matched pair technique male and female quantity 1:1) match in 40 order net cages, Tilapia mossambica is natural mating fertilization in net cage, match 10 days afterwards collection fertilized egg artificial incubation obtain 11 family fries.
3. each family selects 100 tails to obtain the complete male bolti of phenotype according to (1) institute method of telling at random, 100 tails are selected to throw something and feed normal diet as a control group at random, cultivate to about 20g, statistics female-male proportion, finding that there is 6 control group familys is complete female family, the Tilapia mossambica male ratio of these 6 familys after Methyltestosterone process is 100%, and be puppet male Tilapia mossambica family (phenotype male, sex chromosome X X), 3 male bolti familys of puppet of selecting and remain cultivate sexual maturity.
4. utilize 3 male bolti familys of puppet to be male parent, common bolti raun is maternal, wide × long × high at 3m × 10m × 1.5m(respectively) pairing breeding in 40 order net cages, artificially collect fertilized egg and hatch, each family selects 100 tails to obtain the complete male bolti of phenotype according to (1) institute method of telling at random, all the other adopt normal diet cultivation, cultivate to about 20g, statistics female-male proportion, find that the Tilapia mossambica through Methyltestosterone process is male, the Tilapia mossambica of common cultivation is female, obtain complete female Tilapia mossambica 11.7 ten thousand tail altogether, achieve the batch production of the complete female bolti scale production of hybrid seeds and the male bolti of puppet.
Claims (3)
1. pseudo-male and complete female bolti producing method for seed, it is characterized in that, producing method for seed step is as follows:
1) collect bolti fertilized egg to carry out artificial incubation and obtain fry, throw something and feed continuously after initial feeding containing the feed 15 days of Methyltestosterone, obtain the complete male bolti of phenotype and cultivate to sexual maturity;
2) with the male bolti of phenotype for male parent, common female bolti is maternal, matches in net cage according to nido matched pair technique male and female quantity 1:1, collects fertilized egg artificial incubation and obtains bolti seedling;
3) each family selects the 100 tails feed of throwing something and feeding containing Methyltestosterone to obtain phenotype complete male bolti is at random drug treating group, 100 tails normal diet of throwing something and feeding is selected to be control group at random, cultivate to about 20g, the female-male proportion of statistics control group bolti, the drug treating group milter that entirely female control group is corresponding is the male bolti of puppet of sex chromosome X X, and pseudo-male bolti family of selecting and remain cultivates sexual maturity;
4) the offspring's sex chromosome utilizing pseudo-male bolti and common bolti to breed is XX, and common cultivation can realize the complete female bolti scale production of hybrid seeds, carries out androgenic hormone process can be mass pseudo-male bolti to fry.
2. pseudo-male and complete female bolti producing method for seed as claimed in claim 1, it is characterized in that, the batch production of pseudo-male bolti: with the male bolti of puppet for male parent, common female bolti is that maternal breeding obtains fry, by Methyltestosterone process, pseudo-male bolti can be obtained in batches.
3. pseudo-male and complete female bolti producing method for seed as claimed in claim 1; it is characterized in that; the complete female bolti scale production of hybrid seeds: with the male bolti family of puppet for male parent; common female bolti is maternal; the filial generation sex chromosome of breeding is XX type, and common mode cultivation can obtain complete female bolti.
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| CN105145438B (en) * | 2015-09-30 | 2017-12-01 | 中国水产科学研究院淡水渔业研究中心 | The method for establishing bolti merit female pure lines |
| CN108377939B (en) * | 2018-03-13 | 2021-08-06 | 广西壮族自治区水产科学研究院 | Method for producing hybrid Oreochromis aureus |
| CN110338105A (en) * | 2018-04-03 | 2019-10-18 | 嘉丰海洋国际股份有限公司 | The method for inducing the masculine of bolti using the immersion treatment of Jia Ji Testis ketone |
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| CN1177438A (en) * | 1997-09-04 | 1998-04-01 | 中国科学院水生生物研究所 | Method for production of all female carps |
| US6476290B1 (en) * | 1995-04-19 | 2002-11-05 | Dalhousie University | Transgenic tilapia comprising a humanized insulin gene |
| CN101120658A (en) * | 2006-08-08 | 2008-02-13 | 白宝海 | All female method for salmon and bulltrout |
| CN102783445A (en) * | 2012-08-31 | 2012-11-21 | 北京卧佛山庄养殖有限公司 | Method for breeding full-feminization rainbow trout |
| CN102792910A (en) * | 2012-08-31 | 2012-11-28 | 北京市水产科学研究所 | Cultivating method of oncorhynchus mykiss walbaum gynoecial hybrid offspring seed |
| CN102812913A (en) * | 2012-08-31 | 2012-12-12 | 北京市水产科学研究所 | Cultivation method for hybridized all-female golden trout fries |
| CN103070120A (en) * | 2013-01-29 | 2013-05-01 | 水利部中国科学院水工程生态研究所 | Method for establishing full-female pelteobagrus fulvidraco breeding population |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10150883A (en) * | 1996-11-21 | 1998-06-09 | Kanagawa Pref Gov | Method for creating total male triploid fish by using sex reversal male fish of ayu |
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6476290B1 (en) * | 1995-04-19 | 2002-11-05 | Dalhousie University | Transgenic tilapia comprising a humanized insulin gene |
| CN1177438A (en) * | 1997-09-04 | 1998-04-01 | 中国科学院水生生物研究所 | Method for production of all female carps |
| CN101120658A (en) * | 2006-08-08 | 2008-02-13 | 白宝海 | All female method for salmon and bulltrout |
| CN102783445A (en) * | 2012-08-31 | 2012-11-21 | 北京卧佛山庄养殖有限公司 | Method for breeding full-feminization rainbow trout |
| CN102792910A (en) * | 2012-08-31 | 2012-11-28 | 北京市水产科学研究所 | Cultivating method of oncorhynchus mykiss walbaum gynoecial hybrid offspring seed |
| CN102812913A (en) * | 2012-08-31 | 2012-12-12 | 北京市水产科学研究所 | Cultivation method for hybridized all-female golden trout fries |
| CN103070120A (en) * | 2013-01-29 | 2013-05-01 | 水利部中国科学院水工程生态研究所 | Method for establishing full-female pelteobagrus fulvidraco breeding population |
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