CN1177438A - Method for production of all female carps - Google Patents

Method for production of all female carps Download PDF

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Publication number
CN1177438A
CN1177438A CN97109225A CN97109225A CN1177438A CN 1177438 A CN1177438 A CN 1177438A CN 97109225 A CN97109225 A CN 97109225A CN 97109225 A CN97109225 A CN 97109225A CN 1177438 A CN1177438 A CN 1177438A
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carp
female
carps
scattered mirror
scattered
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CN97109225A
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CN1078440C (en
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吴清江
叶玉珍
陈荣德
黄文郁
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Institute of Hydrobiology of CAS
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Institute of Hydrobiology of CAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Feed For Specific Animals (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to the field of pisciculture technology, in particular, it relates to a fish-sex control method. It is characterized by including the following steps: making Sanlinjing carp growth to haptoid through artificial gynogenesis treatment, then reduplicating one to obtain female diploid, feeding the female diploid with methyltestosterome to make it into "physilolgic male" Sanlinjing carp to be used as "male parent", and using pure-line Xingguohong carp as "female parent" to culture hologynic carp. After feeding for 6 months, the weight of one hologynic carp is up to 500-700g, its growth speed is twice that of uncultivated carp, and its fish protein and fat content is 76.66%. Said invention is suitable for production on a certain scale.

Description

The production method of all female carps
The present invention relates to the breed of fish, particularly the sex Artificial Control method of fish.
All there is evident difference in fish because of different its sexual maturity of sex period, growth rate and individual size, growth rate of the male fish that has and individual weight are better than female fish, the female fish that has then is better than male fish, rule in view of the above, people adopt the manually operated method of sex as an important channel of improving fish crop.
The manually operated universal method of the sex of fish is with the sex hormone fingerling of feeding.But can not reach the purpose of female entirely or full hero, and can not entail the next generation, be unfavorable for producing use.
Yang Yongquan equals 1980 at Acta Genetica Sinica 7 (3): deliver " use three series mating and go up complete male Mozambique Tilapia mossambica by way of producing heredity " paper on the 241-246, using that this method can produce all is the complete male offspring of XY type, because after female sex hormone is handled, be difficult to offer an explanation the raun (XY type) of normal raun (XX type) and commentaries on classics property, be difficult to from male offspring, pick out supermale (YY) fish again, thereby can not implement aborning.Jon G.Stanley in 1976 at " Transations of theAmerican Fisheries Society ", Vol.105, deliver " Production of Hybrid; Androgenetic and Gynogenetic Grass Carp andCarp " literary composition on the No.1 pp10-16., reported with ultraviolet ray and made carp sperm genetic inactivation and bring out the method for the full raun of grass carp gynogenesis production, though this method can be produced complete female offspring, but offspring's survival rate only 0.006%, therefore output is extremely low, can not be applied to large-scale production.
The carp that the objective of the invention is widely to culture with China is a research object, creates a kind of method of energy large-scale production all female carps.
In order to realize this purpose, its main technical schemes is: scattered mirror carp is produced monoploid embryo through artificial gynogenesis carry out artificial doubling again, make it produce diploid female, this thelykaryon dliploid all is female, its sex chromosome is the XX type, the female fish of XX type is converted into " physiological male " scattered mirror carp with manual method,, produces all female carps with this " physiological male " scattered mirror carp and the female red carp mating of normal pure lines.
The all female carps that utilizes this technical scheme to produce has following beneficial effect:
1, fast growth, the summer flower fingerling was raised through 6 months then, and cabrage reaches the 500-700 gram, is two times of wild carp.
2, edibility ratio height, because the prematurity still of female sexual gland, thereby edible part accounts for 92% of fish body full weight.
3, quality better, what protein and fat accounted for dry matter weight is 76.66%.
4, raise together with all female carps at the chinese carp fingerling pond, can not increase the feed input, every mu of new units increased in production 15-30 kilogram in fishpond.The main all female carps per mu yield 390-420 kilogram of supporting.
Production method and step:
Embodiment 1,
1, artificial gynogenesis is got the crucian seminal fluid through 10-13 ten thousand rad cobalts 60 gals
Figure A9710922500041
Radiation exposure 1-3 hour or be 200J/cm with intensity 2Ultraviolet irradiation half an hour starts the mature egg of scattered mirror carp with seminal fluid, makes it to carry out gynogenesis;
2, thelykaryon doubling monoploids. ovum starts 5 fens kinds, places 1-3 ℃, and 20-25 minute, or placed 39-41 ℃ of following 1-3 minute, making scattered mirror carp thelykaryon doubling monoploids is the thelykaryon dliploid;
3, the Artificial Control of Sex Differentiation direction, beginning to feed after fry emerged 20 days contained the artificial feed of the Methyltestosterone of 30ppm in 90 days, made to transfer " physiological male " scattered mirror carp to;
4, after manually " physiological male " scattered mirror carp of commentaries on classics property is bred parent population, with this parent population and the female red carp of making the country prosperous of pure lines.Producing according to a conventional method all is female all female carps fish.
Embodiment 2,
The gynoecy scattered mirror carp is produced in method acquisition " physiological male " scattered mirror carp and female scattered mirror carp mating with embodiment 1, press the 3rd method of embodiment 1 again and handle a large amount of " physiological male " scattered mirror carp of acquisition, do " male parent " and the female red carp mating of making the country prosperous of pure lines with this, produce all female carps fish.

Claims (3)

1, a kind of Artificial Control fish sex, the production method of all female carps is characterized in that:
A, get the crucian seminal fluid, start the mature egg of scattered mirror carp, make it gynogenesis with the seminal fluid of this processing through cobalt gamma ray or ultraviolet irradiation; Ovum starts 5 fens kinds, places 1-3 ℃, and 20-25 minute, or placed 39-41 ℃ of following 1-3 minute, making scattered mirror carp thelykaryon doubling monoploids is gynogenesis diploid; Beginning to feed after fry emerged 20 days contained the artificial feed of the Methyltestosterone of 30ppm in 90 days, made to transfer " physiological male " scattered mirror carp to, with itself and pure Xingguo red carp mating, produced all female carps again;
B, get " physiological male " scattered mirror carp and female scattered mirror carp mating and produce the gynoecy scattered mirror carp, contained the artificial feed of the Methyltestosterone of 30ppm again in 90 days through feeding, obtain a large amount of " physiological male " scattered mirror carps, with the pure Xingguo red carp mating. produce all female carps.
2, the production method of all female carps according to claim 1 is characterized in that cobalt 60 gals The exposure intensity of ray is 10-13 ten thousand rads, time 1-3 hour.
3, the production method of all female carps according to claim 1 is characterized in that ultraviolet irradiation intensity is 200J/cm 2, half an hour time.
CN97109225A 1997-09-04 1997-09-04 Method for production of all female carps Expired - Fee Related CN1078440C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN97109225A CN1078440C (en) 1997-09-04 1997-09-04 Method for production of all female carps

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN97109225A CN1078440C (en) 1997-09-04 1997-09-04 Method for production of all female carps

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CN1078440C CN1078440C (en) 2002-01-30

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100435630C (en) * 2007-01-27 2008-11-26 中国水产科学研究院黄海水产研究所 Cynoglossus semilaevis ovum miosis gynogenesis method induced by bass frozen sperm
CN103583427A (en) * 2013-11-06 2014-02-19 广西壮族自治区水产科学研究院 Pseudo-male and full-female nile tilapia fingerling production method
CN104285863A (en) * 2014-10-21 2015-01-21 浙江省海洋水产研究所 Method for inducing pseudo milters of gynogenesis spotted maigre
CN110771536A (en) * 2019-11-21 2020-02-11 浙江省海洋水产研究所 Large-scale cultivation method of all-female spotted maigre

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100539832C (en) * 2007-08-28 2009-09-16 中国水产科学研究院黄海水产研究所 The method of inducing female nucleus growth with heterologous frozen sperm

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100435630C (en) * 2007-01-27 2008-11-26 中国水产科学研究院黄海水产研究所 Cynoglossus semilaevis ovum miosis gynogenesis method induced by bass frozen sperm
CN103583427A (en) * 2013-11-06 2014-02-19 广西壮族自治区水产科学研究院 Pseudo-male and full-female nile tilapia fingerling production method
CN103583427B (en) * 2013-11-06 2015-09-23 广西壮族自治区水产科学研究院 Pseudo-male and complete female bolti producing method for seed
CN104285863A (en) * 2014-10-21 2015-01-21 浙江省海洋水产研究所 Method for inducing pseudo milters of gynogenesis spotted maigre
CN104285863B (en) * 2014-10-21 2016-08-24 浙江省海洋水产研究所 A kind of method inducing gynogenesis Nibea albiflora puppet milter
CN110771536A (en) * 2019-11-21 2020-02-11 浙江省海洋水产研究所 Large-scale cultivation method of all-female spotted maigre
CN110771536B (en) * 2019-11-21 2021-09-14 浙江省海洋水产研究所 Large-scale cultivation method of all-female spotted maigre

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