CN108244000A - The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized - Google Patents
The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized Download PDFInfo
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- CN108244000A CN108244000A CN201810125264.8A CN201810125264A CN108244000A CN 108244000 A CN108244000 A CN 108244000A CN 201810125264 A CN201810125264 A CN 201810125264A CN 108244000 A CN108244000 A CN 108244000A
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- dai
- crocea
- days
- pseudosciaena crocea
- feminized
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- 241001596950 Larimichthys crocea Species 0.000 title claims abstract description 33
- 238000013459 approach Methods 0.000 title claims abstract description 7
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims abstract description 9
- 229960005309 estradiol Drugs 0.000 claims abstract description 9
- 229930182833 estradiol Natural products 0.000 claims abstract description 9
- 230000009466 transformation Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 210000004907 gland Anatomy 0.000 claims description 3
- 230000001568 sexual effect Effects 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims description 2
- 241000251468 Actinopterygii Species 0.000 abstract description 11
- 230000006698 induction Effects 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 241001417494 Sciaenidae Species 0.000 abstract description 3
- 230000004075 alteration Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 235000008078 Arctium minus Nutrition 0.000 description 1
- 241000721191 Clarias gariepinus Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 240000004980 Rheum officinale Species 0.000 description 1
- 235000008081 Rheum officinale Nutrition 0.000 description 1
- 240000005374 Rumex hydrolapathum Species 0.000 description 1
- 235000015290 Rumex hymenosepalus Nutrition 0.000 description 1
- 241000630329 Scomberesox saurus saurus Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001061 forehead Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/565—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids not substituted in position 17 beta by a carbon atom, e.g. estrane, estradiol
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Public Health (AREA)
- Marine Sciences & Fisheries (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses the abductive approach that Dai-ju stock Pseudosciaena crocea is feminized, with estradiol 40 45 age in days Pseudosciaena crocea Fries are carried out with the induction production of hybrid seeds 42 days, it can be obtained by transformation system function raun, such method induction fish, which feminize, to be had many advantages, such as that female conversion ratio and survival rate are high, operate that easy, dosage is few, processing time is short and not aberration inducing.Basis and preposition technology can be provided for Larimichthys crocea and the nearlyer Sciaenidae unisexuality breeding of other affiliations.
Description
Technical field
The present invention relates to the breeding techniques of Larimichthys crocea, and in particular to the abductive approach that Dai-ju stock Pseudosciaena crocea is feminized.
Background technology
Larimichthys crocea (Larimichthys crocea), Perciformes, Sciaenidae, yellow croaker category are belonged in classification, is that cluster is whirled
The warm warm nature near sea fishes of trip, are mainly distributed on the marine sites such as China's the East China Sea, the Taiwan Straits and the South Sea.Cultivation at present
Larimichthys crocea mainly has 2 groups, and respectively Dai-ju stock Pseudosciaena crocea and east Guangdong Larimichthys crocea, Dai-ju stock Pseudosciaena crocea is distributed in because of it
The Dai Quyang and Lv Siyang of Zhoushan Fishing Ground and gain the name, have compared with east Guangdong Larimichthys crocea in the market body colour is yellow, build is good,
The features such as meat is excellent, popular with consumers, commodity value is high.With the gradually subdivision of Larimichthys crocea consumption market and secondary industry,
The presented trend for having moved towards different market channels of the Larimichthys crocea of different sexes, this is not only due to Larimichthys crocea male build more
It is good, directly as high-end gift fish and food and drink good merchantable brand, female individuals can utilize roe to develop deep processed product, more with consumer couple
The history sincerity of Wild Rhubarb fish and consumer form directly related.Rheum officinale fish culture and process mating industry have been equipped with carrying out
The Industry Foundation and the market demand of Larimichthys crocea monosex cultivation.At present, fish breeding has started based on fish sex and has determined that pattern is opened
The production of hybrid seeds of bill fish and breeding cultivation, receive the concern of aquatic products circle.But its production of hybrid seeds work originates in the induction of physiology Dot Blots Sex,
The large-scale production of unisexuality breeding could be realized by only realizing artificial sex induction.The research converted with hormone induction fish property
It starts from after estradiol is successfully synthesized and puts into production soon；According to display is had been reported, China is in Tilapia mossambica, yellow forehead
Sex induction research experiment is carried out in the cultured fishes such as fish, C. lazera, since kind is different, development time is different, processing
Method is different, and integrated conduct method is there are great difference, and induced efficiency is also significantly different.
Invention content
The technical problems to be solved by the invention are to provide the abductive approach that a kind of Dai-ju stock Pseudosciaena crocea is feminized, the induction
Method has many advantages, such as that female conversion ratio and survival rate are higher, processing time is short and not aberration inducing.
Technical solution is used by the present invention solves above-mentioned technical problem：The induction side that Dai-ju stock Pseudosciaena crocea is feminized
Method, its step are as follows：
A, estradiol with scrod feed is uniformly mixed, is configured to the pharmaceutical chemistry that estradiol content is 80~120mg/kg；
B, will hatching after 40~45 ages in days, body long size be 18~20mm, the above-mentioned pharmaceutical chemistry of the undifferentiated Pseudosciaena crocea Fry of sexual gland
It is fed 42 days instead of scrod feed, 22~25 DEG C of water temperature, salinity 22~24,1000~1500lx of illumination, pharmaceutical chemistry stop during feeding
Normal feeding management is carried out after feeding, you can obtains transformation system function raun.
Compared with prior art, the advantage of the invention is that the abductive approach that Dai-ju stock Pseudosciaena crocea is feminized, uses estradiol
40~45 age in days Pseudosciaena crocea Fries are carried out with the induction production of hybrid seeds 42 days, with regard to Ke to obtain transformation system function raun, such method induces
Fish feminize with female conversion ratio and survival rate be high, operation easily, dosage is few, processing time is short and aberration inducing etc. is not excellent
Point.Basis and preposition technology can be provided for Larimichthys crocea and the nearlyer Sciaenidae unisexuality breeding of other affiliations.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1
120mg estradiol is added to after the dissolving of 10ml ethyl alcohol as hormone working solution in 50ml ultra-pure waters, then weigh 1kg childrens
Fish meal is put in pallet, and the hormone working solution dissolved with estradiol is uniformly sprayed scrod feed, makes its final concentration of
The pharmaceutical chemistry of 120mg/kg is placed in 37 DEG C of drying 2h in baking oven, until ethyl alcohol volatilization packs refrigeration completely, after pharmaceutical chemistry drying.It chooses
The Dai-ju stock Pseudosciaena crocea fry of 40 ages in days 10000 feeds feed with pharmaceutical chemistry, and the time fed daily is 7：00、11:00、
15:00、20：00 point, feeding volume, which was subject in half an hour, to be eaten up, and it is primary respectively to change water sooner or later, quantity of exchanged water 50%-80%, continuous days
42 days.Daily management during nursery carries out without exception according to the method for Larimichthys crocea artificial propagation.Fry is shifted after induction
It is formed to marine net cage, chow diet is fed during forming, once in the morning and once at night, day feeding volume is the 3% of fish body weight.To big
The identification of yellow croaker gender mainly takes sexual gland to be determined with Histological method, and the property for feeding 150 days or so is taken to turn 31 tail of Pseudosciaena crocea Fry
（Body grows 14.00 ~ 23.5cm）, it transports laboratory back and is dissected, gonadal tissue is taken to carry out microexamination, distinguishes male and female for the first time, it is female
Property rate be 96.77%.Quadratic search physiological raun genetic sex takes muscle of back tissue to carry out molecular specificity verification, specifically
Including DNA extractions-PCR amplification-agarose electrophoresis, as a result androgenetic individual ratio is 56.67% in physiological raun.
Embodiment 2
It is substantially the same manner as Example 1, the pharmaceutical chemistry of different only final concentration of 80mg/kg, selection be 45 ages in days Dai Qu
Race's Pseudosciaena crocea Fry.
Embodiment 3
It is substantially the same manner as Example 1, the pharmaceutical chemistry of different only final concentration of 100mg/kg, selection be 42 ages in days Dai Qu
Race's Pseudosciaena crocea Fry.
Claims (1)
1. the abductive approach that Dai-ju stock Pseudosciaena crocea is feminized, it is characterised in that step is as follows：
A, estradiol with scrod feed is uniformly mixed, is configured to the pharmaceutical chemistry that estradiol content is 80~120mg/kg, preferably
120mg/kg；
B, will hatching after 40~45 ages in days, body long size be 18~20mm, the above-mentioned pharmaceutical chemistry of the undifferentiated Pseudosciaena crocea Fry of sexual gland
It is fed 42 days instead of scrod feed, 22~25 DEG C of water temperature, salinity 22~24,1000~1500lx of illumination, pharmaceutical chemistry stop during feeding
Normal feeding management is carried out after feeding, you can obtains transformation system function raun.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810125264.8A CN108244000A (en) | 2018-02-08 | 2018-02-08 | The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810125264.8A CN108244000A (en) | 2018-02-08 | 2018-02-08 | The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108244000A true CN108244000A (en) | 2018-07-06 |
Family
ID=62744029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810125264.8A Pending CN108244000A (en) | 2018-02-08 | 2018-02-08 | The abductive approach that Dai-ju stock Pseudosciaena crocea is feminized |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108244000A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406165A (en) * | 2008-11-25 | 2009-04-15 | 广州鹭业水产有限公司 | Method for producing seeds of ultra-male Niao tilapia |
| CN101416613A (en) * | 2008-11-25 | 2009-04-29 | 广州鹭业水产有限公司 | The method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote |
| CN101449664A (en) * | 2008-11-25 | 2009-06-10 | 广州鹭业水产有限公司 | Seed production method of WZ(male)-ZZ(female) all-male blue tilapia |
| CN105377025A (en) * | 2013-06-15 | 2016-03-02 | 古高尔控股有限公司 | Method for inducing artificial ovulation and spawning of fresh-water eels |
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
-
2018
- 2018-02-08 CN CN201810125264.8A patent/CN108244000A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406165A (en) * | 2008-11-25 | 2009-04-15 | 广州鹭业水产有限公司 | Method for producing seeds of ultra-male Niao tilapia |
| CN101416613A (en) * | 2008-11-25 | 2009-04-29 | 广州鹭业水产有限公司 | The method of feminizing of WZ ♀-ZZ ♂ type Oreochromis aureus ZZ zygote |
| CN101449664A (en) * | 2008-11-25 | 2009-06-10 | 广州鹭业水产有限公司 | Seed production method of WZ(male)-ZZ(female) all-male blue tilapia |
| CN105377025A (en) * | 2013-06-15 | 2016-03-02 | 古高尔控股有限公司 | Method for inducing artificial ovulation and spawning of fresh-water eels |
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180706 |