CN109480125A - Universal solid shell additive of one seed shrimp crab and its preparation method and application - Google Patents

Universal solid shell additive of one seed shrimp crab and its preparation method and application Download PDF

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Publication number
CN109480125A
CN109480125A CN201811282000.XA CN201811282000A CN109480125A CN 109480125 A CN109480125 A CN 109480125A CN 201811282000 A CN201811282000 A CN 201811282000A CN 109480125 A CN109480125 A CN 109480125A
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CN
China
Prior art keywords
shell
shrimp
vitamin
shrimp crab
additive
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Pending
Application number
CN201811282000.XA
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Chinese (zh)
Inventor
张其华
郑石轩
姚春凤
程开敏
黎春昶
康裕财
刘丽燕
朱学芝
马学坤
桑勇
刘建军
刘娟花
胡蝶
李素莹
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ZHEJIANG YUEHAI FEED CO Ltd
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ZHEJIANG YUEHAI FEED CO Ltd
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Priority to CN201811282000.XA priority Critical patent/CN109480125A/en
Publication of CN109480125A publication Critical patent/CN109480125A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements

Abstract

The present invention relates to technical field of aquaculture, more particularly to universal solid shell additive of a seed shrimp crab and preparation method thereof.The universal solid shell additive of shrimp crab provided by the invention is made of the component of following mass percent: shrimp shell meal 25%~30%, squid visceral meal 15%~20%, calcium dihydrogen phosphate 25%~30%, ferrous sulfate 0.5%~1%, cupric glycinate 1%~2%, zinc methionine 2%~3%, Mn-Gly 1%~2%, astaxanthin 1%~2%, cholesterol 3%~5%, chitosan 4%~6%, the vitamin C phosphoric ester 5%~8% of content 35%, vitamine D3 0.5%~1%, vitamin e1 %~2%.The universal solid shell additive of shrimp crab of the present invention can improve the problems such as unsuccessful soft shell in shrimp crab breeding process, shell and solid shell, effectively improve shrimp crab survival rate, enhance shrimp crab immunity and resistance, promote growth.

Description

Universal solid shell additive of one seed shrimp crab and its preparation method and application
Technical field
The present invention relates to technical field of aquaculture, more particularly to the universal solid shell additive of a seed shrimp crab and its preparation Method.
Background technique
Shrimp crab is as main crustacean, and cultivation development in recent years at home is swift and violent, and China's seawater shrimps are supported within 2016 It grows yield and reaches 1271743 tons, increased by 9.51% than 2015;Sea crabs cultured output reaches 292850 tons, increases than 2015 Add 7.04%;China's Freshwater Shrimp Aquiculture yield reaches 2032113 tons within 2016, increases by 8.82% than 2015;Fresh water crab cultivation Yield is 812103 tons, slightly subtracts 1.36% than 2015;China's seawater and freshwater shrimp crab cultivation total output reaches within 2016 4408809 tons, form sizable cultivation scale.With the increase of shrimp crab cultivation scale, also there are many cultivation problems, such as It is more typical general character growth question during prawn and crab cultivation that soft shell disease, shell be unsuccessful etc., influences prawn and crab is supported The survival rate grown, the reason of causing these problems, has with existing aquaculture model and feed formula structure compared with Important Relations, in fresh water Cultivation is alternately injected in sea-farming using sea water and fresh water, and salinity is lower in breeding water body, and the cultivation of shrimp crab is in the shell phase to dimension The demand that raw element, mineral element etc. are conducive to solid shell component changes greatly, and commercial feed common at present is not met by shrimp The nutritional need of the special growth period of crab.
Summary of the invention
The purpose of the present invention is to the disadvantages and deficiency in existing feed technology, provide a seed shrimp crab universal solid shell Additive solves the common problems such as the soft shell disease occurred in shrimp crab breeding process, shell be unsuccessful, it is ensured that shrimp crab is in the shell phase pair The composition requirements amount such as vitamin, mineral element guarantees the growth result of shrimp crab particular time, improves survival rate, and also can be used as The effective component of forage protein is added.
Another object of the present invention is to provide the preparation methods and application method of the universal solid shell additive of above-mentioned shrimp crab.
To achieve the above object, the present invention is realized according to following technical scheme:
The universal solid shell additive of shrimp crab provided by the invention, shrimp shell meal 25%~30%, squid visceral meal 15%~ 20%, calcium dihydrogen phosphate 25%~30%, ferrous sulfate 0.5%~1%, cupric glycinate 1%~2%, zinc methionine 2%~ 3%, Mn-Gly 1%~2%, astaxanthin 1%~2%, cholesterol 3%~5%, chitosan 4%~6%, content 35% Vitamin C phosphoric ester 5%~8%, vitamine D3 0.5%~1%, vitamin e1 %~2%.
Wherein, the chemical name of the astaxanthin is 3,3 '-dihydroxy -4,4 '-diketo-β, β '-carrotene, chemistry Molecular formula is C40H52O4
Preferably, the sum of above-mentioned each component mass percent is 100%.
The preparation method of the universal solid shell additive of shrimp crab provided by the invention, which comprises the following steps:
S1 weighs above-mentioned each substance component according to mass percent;
Each substance component weighed in S1 is ground into powders component by S2;
Powders component obtained in S2 is uniformly mixed by S3.
Preferably, the order by merging in the step S3 is followed successively by shrimp shell meal, squid visceral meal, calcium dihydrogen phosphate, sulfuric acid Ferrous iron, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, chitosan, content 35% vitamin C phosphoric ester, Vitamin D3, vitamin E, the substance component is uniformly mixed.
Preferably, the order by merging in the step S3 is followed successively by shrimp shell meal, squid visceral meal, calcium dihydrogen phosphate, sulfuric acid Ferrous iron, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, chitosan, content 35% vitamin C phosphoric ester, Vitamin D3, vitamin E.
Preferably, powders component described in step S2 is to be crushed to 100 mesh, that is, is sieved with 100 mesh sieve;It is uniformly mixed in step S3 When, the substance each component mixture homogeneity coefficient of variation≤5%.
The application method of additive provided by the invention is as follows:
1, add in shrimp crab mixed feed production process with account for shrimp crab mixed feed mass percent 1.5~2.0% Dosage addition, forms the shrimp crab mixed feed finished product containing the additive, for cultivating shrimp crab.
2, spice uses, and the mass ratio of 1:50 is pressed with general goods shrimp crab mixed feed, and uniform spice is again before feeding It feeds.
The present invention has the advantage that
1, the present invention has used the group of the Multicomponents such as shrimp shell meal and astaxanthin, vitamin, minerals, chitosan for the first time It closes, is taken by addition shrimp shell meal, squid visceral meal, calcium dihydrogen phosphate, astaxanthin and multivitamin, the reasonable of several mineral materials Match, can be sloughed off in shrimp crab and change the particular time of shell required full nutrition is provided for it, by adding chitosan, shrimp can be effectively improved Crab changes the immunity and premunition of shell particular time sloughing off, and guarantees that shrimp crab sloughs off and changes shell smoothly and normal growth.Addition of the invention Just sufficiently research shrimp crab is on the basis of sloughing off the nutritional need for changing shell particular time for agent prescription, than pervious feed addictive prescription It is more accurate reasonable.
2, the present invention can improve the problems such as unsuccessful soft shell in shrimp crab breeding process, shell and solid shell, effectively improve shrimp crab Survival rate enhances shrimp crab immunity and resistance, promotes growth.
3, shrimp shell meal, squid visceral meal and multivitamin, mineral combinations are had good attractant effect by the present invention, The palatability of feed can be improved, while shrimp shell meal, minerals and protein in squid visceral meal can be made full use of.
4, the present invention used in primary raw material shrimp shell meal, squid visceral meal be processing of aquatic products waste, steady sources, The high level for being able to achieve narrow resources through the present invention efficiently utilizes.
Specific embodiment
Below with reference to embodiment, the invention will be further described:
In following embodiment the chemical name of astaxanthin be 3,3 '-dihydroxy -4,4 '-diketo-β, β '-carrotene, Chemical molecular formula is C40H52O4
Embodiment one
(1) 26 kilograms of shrimp shell meals are weighed, 16 kilograms of squid visceral meals, 26 kg of phosphoric acid calcium dihydrogens, 1 kilogram of ferrous sulfate, 2 Kilogram cupric glycinate, 3 kilograms of zinc methionines, 2 kilograms of Mn-Glies, 2 kilograms of astaxanthins, 5 kilograms of cholesterol, 6 kilograms of chitosans, The vitamin C phosphoric ester of 8 kilograms of contents 35%, 1 kilogram of vitamin D3, 2 kilograms of vitamin Es.
(2) the above component is crushed respectively, 100 mesh of smashing fineness sieves with 100 mesh sieve.
(3) shrimp shell meal to pulverize and sieve is put into mixing machine, then successively puts into the squid visceral meal to pulverize and sieve, phosphoric acid Calcium dihydrogen, ferrous sulfate, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, chitosan, content 35% dimension Raw element C phosphate, vitamine D3, vitamin E, the substance component is uniformly mixed, it is ensured that the variation of each component mixture homogeneity Coefficient≤5%;Or the cuttlefish to pulverize and sieve is then first put by any order in the shrimp shell meal investment mixing machine to pulverize and sieve Visceral meal, ferrous sulfate, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, chitosan, contains calcium dihydrogen phosphate Vitamin C phosphoric ester, the vitamine D3, vitamin E of amount 35%, then the substance component is uniformly mixed, it is ensured that each component is mixed Close the uniformity coefficient of variation≤5%;It can be prepared by the universal solid shell additive of shrimp crab.
Embodiment two
Weigh 30 kilograms of shrimp shell meals, 20 kilograms of squid visceral meals, 30 kg of phosphoric acid calcium dihydrogens, 0.5 kilogram of ferrous sulfate, 1 Kilogram cupric glycinate, 2 kilograms of zinc methionines, 1 kilogram of Mn-Gly, 1 kilogram of astaxanthin, 3 kilograms of cholesterol, 4 kilograms of chitosans, The vitamin C phosphoric ester of 6 kilograms of contents 35%, 0.5 kilogram of vitamine D3,1 kilogram of vitamin E.Remaining preparation method is by implementation The universal solid shell additive of shrimp crab can be made in example one.
Embodiment three
Weigh 28 kilograms of shrimp shell meals, 18.5 kilograms of squid visceral meals, 28 kg of phosphoric acid calcium dihydrogens, 0.75 kilogram of sulfuric acid Asia Iron, 1.5 kilograms of cupric glycinates, 2.5 kilograms of zinc methionines, 1.5 kilograms of Mn-Glies, 1.5 kilograms of astaxanthins, 4 kilograms of cholesterol, 5 kilograms of chitosans, the vitamin C phosphoric ester of 6.5 kilograms of contents 35%, 0.75 kilogram of vitamine D3,1.5 kilograms of vitamin Es.Its The universal solid shell additive of shrimp crab can be made by embodiment one in remaining preparation method.
By upper described, the present invention can be better realized.
The invention product is tested for prawn culturing: the universal solid shell additive of above-mentioned shrimp crab is added in the ratio of 1:50 Spice in the general goods penaeus vannamei boone feed produced to our company carries out culture experiment.Control group is not plus shrimp crab is general Type consolidates the same model general goods penaeus vannamei boone feed of shell additive.Select health, specification uniformly, initial weight be about 0.45 gram Litopenaeus vannamei is tested, and 4 processing, each processing sets 4 repetitions, and each Glass fibre reinforced plastic tub (300 liters) puts 40 tail of prawn in a suitable place to breed, Culture experiment 8 weeks by a definite date, remaining was consistent in addition to feed, and the litopenaeus vannamei cultivation result of embodiment and control group is seen below Table:
Item number First counterpoise Last counterpoise Rate of body weight gain Feed coefficient Survival rate
Control group 0.45±0 11.48±0.06 2451.85±13.71 1.46±0.01b 67.5±1.44a
Embodiment 1 0.45±0 11.71±0.04 2502.22±9.25 1.17±0.01a 91.67±2.2b
Embodiment 2 0.45±0 11.63±0.06 2483.71±14.19 1.23±0.02a 87.5±2.5b
Embodiment 3 0.45±0 11.7±0.05 2500.74±10.29 1.2±0.02a 87.5±1.44b
F . 3.773 3.772 58.321 30.955
P . 0.059 0.059 0.000 0.000
The experimental results showed that embodiment each group rate of body weight gain is slightly above control group, but group difference is not significant (P > 0.05);And The survival rate of embodiment each group is significantly higher than control group (P < 0.05), and feed coefficient is substantially less than control group (P < 0.05), implements Survival rate and feed coefficient difference is not significant (P > 0.05) between example each group.
The experimental results showed that breeding process whole process, using the universal solid shell additive of shrimp crab prepared by the present invention, shrimp shell is suitable Benefit has prevented soft shell, the generation for phenomena such as shell is unsuccessful, to enhancing shrimp immunity and premunition, improve survival rate have it is significant Effect, also can significantly reduce feed coefficient, to reduce shrimp crab aquaculture cost.
The invention product is tested for Eriocheir sinensis: using general goods Chinese eriocheir sinensises feed as control group, being added In addition stating the general goods Chinese eriocheir sinensises feed of the universal solid shell additive (carrying out spice in the ratio of 1:50) of three seed shrimp crabs As test group, cultivation comparative test is carried out.4 groups of feeds, every group of feed set 3 repetitions, select health, specification uniformly, initial weight About 0.56 gram of Eriocheir sinensis is tested, and each Glass fibre reinforced plastic tub (300 liters) puts 20 tail of Eriocheir sinensis in a suitable place to breed, and culture experiment is 6 weeks, remaining condition was completely the same in addition to feed, and the cultivation result of test group and control group Eriocheir sinensis see the table below:
Item number First counterpoise Last counterpoise (g) Rate of body weight gain (%) Feed coefficient Survival rate (%)
Control 0.56±0 3.69±0.02 558.93±4.12 1.96±0b 63.33±1.67a
Test 1 0.56±0 3.76±0.03 571.43±4.49 1.93±0.01a 78.33±1.67b
Test 2 0.56±0 3.75±0.02 569.05±3.62 1.93±0.01a 80±2.89b
Test 3 0.56±0 3.73±0.04 566.67±6.3 1.93±0.01a 80±5b
F . 1.306 1.306 7.690 6.738
P . 0.338 0.338 0.010 0.014
The experimental results showed that test group each group rate of body weight gain is slightly above control group, but group difference is not significant (P > 0.05);And The survival rate of test group each group is significantly higher than control group (P < 0.05);Feed coefficient is substantially less than control group (P < 0.05), test Survival rate and feed coefficient difference is not significant (P > 0.05) between group each group.
The experimental results showed that breeding process whole process uses the universal solid shell additive of shrimp crab prepared by the present invention, Eriocheir sinensia Crab shell is smooth, has prevented soft shell, the generation for phenomena such as shell is unsuccessful, to the immunity and premunition of enhancing crab, raising is survived Rate has obvious action, also can significantly reduce feed coefficient, to reduce the aquaculture cost of crab.
The above is presently preferred embodiments of the present invention, but the present invention should not be limited to disclosed in the embodiment Content.So all do not depart from the lower equivalent or modification completed of spirit disclosed in this invention, the model that the present invention protects is both fallen within It encloses.

Claims (9)

1. the universal solid shell additive of a seed shrimp crab, the additive are made of the component of following mass percent: shrimp shell meal 25%~30%, squid visceral meal 15%~20%, calcium dihydrogen phosphate 25%~30%, ferrous sulfate 0.5%~1%, sweet ammonia Sour copper 1%~2%, zinc methionine 2%~3%, Mn-Gly 1%~2%, astaxanthin 1%~2%, cholesterol 3%~5%, Chitosan 4%~6%, the vitamin C phosphoric ester 5%~8% of content 35%, vitamine D3 0.5%~1%, vitamin e1 % ~2%.
2. additive according to claim 1, which is characterized in that the chemical name of the astaxanthin is 3,3 '-dihydroxy- 4,4 '-diketo-β, β '-carrotene, chemical molecular formula C40H52O4
3. additive according to claim 1, which is characterized in that the sum of described each component mass percent is 100%.
4. the preparation method of -3 any additives according to claim 1, which comprises the following steps:
S1 weighs above-mentioned each substance component according to mass percent;
Each substance component weighed in S1 is ground into powders component by S2;
Powders component obtained in S2 is uniformly mixed by S3.
5. the preparation method according to claim 4, which is characterized in that the order by merging in the step S3 is followed successively by shrimp shell Powder, squid visceral meal, calcium dihydrogen phosphate, ferrous sulfate, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, shell The substance component is uniformly mixed by glycan, the vitamin C phosphoric ester of content 35%, vitamine D3, vitamin E.
6. the preparation method according to claim 4, which is characterized in that the order by merging in the step S3 is followed successively by shrimp shell Powder, squid visceral meal, calcium dihydrogen phosphate, ferrous sulfate, cupric glycinate, zinc methionine, Mn-Gly, astaxanthin, cholesterol, shell Glycan, the vitamin C phosphoric ester of content 35%, vitamine D3, vitamin E.
7. the preparation method according to claim 4, which is characterized in that powders component described in step S2 is to be crushed to 100 Mesh sieves with 100 mesh sieve;When being uniformly mixed in step S3, the substance each component mixture homogeneity coefficient of variation≤5%.
8. the application method of -3 any additives according to claim 1, which is characterized in that raw in shrimp crab mixed feed 1.5~2.0% additive amount addition of shrimp crab mixed feed mass percent is accounted for during production, formation contains the additive Shrimp crab mixed feed finished product, for cultivating shrimp crab.
9. the application method of -3 any additives according to claim 1, which is characterized in that spice uses, and common Commercial sized prawn crab mixed feed presses the mass ratio of 1:50, and uniform spice is fed again before feeding.
CN201811282000.XA 2018-10-31 2018-10-31 Universal solid shell additive of one seed shrimp crab and its preparation method and application Pending CN109480125A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110002568A (en) * 2018-10-09 2019-07-12 江门市澳保生物科技有限公司 A kind of water-soluble Ca and Mg regulator and preparation method thereof
CN112471357A (en) * 2020-12-03 2021-03-12 珠海容川饲料有限公司 Penaeus monodon shell fixing compound feed and application thereof
CN112655846A (en) * 2020-12-25 2021-04-16 长沙兴嘉生物工程股份有限公司 Organic trace element compound bag for shrimps and crabs as well as preparation method and application of organic trace element compound bag

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Publication number Priority date Publication date Assignee Title
CN101518299A (en) * 2008-05-29 2009-09-02 珠海市世海饲料有限公司 Formulated hatch feed of young penaeus
CN101904434A (en) * 2009-10-23 2010-12-08 广东粤海饲料集团有限公司 Microbial growth regulator for prawn culture and preparation method thereof
CN102715378A (en) * 2012-07-05 2012-10-10 广东粤佳饲料有限公司 Composite shell strengthening additive for litopenaeus vannamei and preparation method thereof
CN102715379A (en) * 2012-07-05 2012-10-10 广东粤佳饲料有限公司 Compound shell reinforcing additive for sugpo prawns and preparation method of compound shell reinforcing additive
CN103704519A (en) * 2013-12-24 2014-04-09 广东粤海饲料集团有限公司 Chinese herbal anti-disease feed additive for prawns and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101518299A (en) * 2008-05-29 2009-09-02 珠海市世海饲料有限公司 Formulated hatch feed of young penaeus
CN101904434A (en) * 2009-10-23 2010-12-08 广东粤海饲料集团有限公司 Microbial growth regulator for prawn culture and preparation method thereof
CN102715378A (en) * 2012-07-05 2012-10-10 广东粤佳饲料有限公司 Composite shell strengthening additive for litopenaeus vannamei and preparation method thereof
CN102715379A (en) * 2012-07-05 2012-10-10 广东粤佳饲料有限公司 Compound shell reinforcing additive for sugpo prawns and preparation method of compound shell reinforcing additive
CN103704519A (en) * 2013-12-24 2014-04-09 广东粤海饲料集团有限公司 Chinese herbal anti-disease feed additive for prawns and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110002568A (en) * 2018-10-09 2019-07-12 江门市澳保生物科技有限公司 A kind of water-soluble Ca and Mg regulator and preparation method thereof
CN110002568B (en) * 2018-10-09 2021-09-17 江门市澳保生物科技有限公司 Water-soluble calcium-magnesium regulator and preparation method thereof
CN112471357A (en) * 2020-12-03 2021-03-12 珠海容川饲料有限公司 Penaeus monodon shell fixing compound feed and application thereof
CN112655846A (en) * 2020-12-25 2021-04-16 长沙兴嘉生物工程股份有限公司 Organic trace element compound bag for shrimps and crabs as well as preparation method and application of organic trace element compound bag
CN112655846B (en) * 2020-12-25 2023-10-31 长沙兴嘉生物工程股份有限公司 Organic trace element composite package for shrimps and crabs as well as preparation method and application thereof

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Application publication date: 20190319