CN105409857A - Fingerling-preparing method for pure-line yellow barbus capito - Google Patents

Fingerling-preparing method for pure-line yellow barbus capito Download PDF

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Publication number
CN105409857A
CN105409857A CN201510962779.XA CN201510962779A CN105409857A CN 105409857 A CN105409857 A CN 105409857A CN 201510962779 A CN201510962779 A CN 201510962779A CN 105409857 A CN105409857 A CN 105409857A
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CN
China
Prior art keywords
yellow
squama barb
large squama
silver gray
barb
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CN201510962779.XA
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Chinese (zh)
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CN105409857B (en
Inventor
徐伟
耿龙武
姜海峰
李晨宇
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Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
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Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences
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Priority to CN201510962779.XA priority Critical patent/CN105409857B/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a fingerling-preparing method for pure-line yellow barbus capito. The invention provides a breeding method for pure-line yellow barbus capito. The method comprises the following steps: firstly selecting wild silver gray barbus capito in a natural water area to be cultured till gonadal maturation; then performing cross breeding; and finally obtaining yellow barbus capito individuals. The method provided by the invention is applied to the field of fingerling preparation for barbus capito.

Description

A kind of producing method for seed being sheerly yellow large squama Barb
Technical field
The present invention relates to a kind of producing method for seed being sheerly yellow large squama Barb.
Background technology
Large squama Barb (Barbuscapito), belongs to Cyprinidae, Barbinae, Barb belongs to, and the river being mainly distributed in the Caspian Sea of West Asia and the Saltwater Sea and flowing through is local famous and precious large-scale economic fish.This fish length can reach 70cm, body weight 12kg, and merits such as having that Salt And Alkali Tolerance is high, feeding habits are wide, delicious meat, growth are fast, does not still carry out artificial breeding research abroad, still locate the wild reset condition under natural water area.2003 under the support of the Ministry of Agriculture " 948 " project; domesticly introduce wild large squama Barb from Uzbekistan first; through research and the test of Heilungkiang aquatic products research institute scientific research personnel more than ten years; whole artificial propagation and the large-scale production of this fish is achieved first in China; and carried out cultivating promoting in multiple provinces and cities, create good economy, society and ecological benefits.The large squama Barb body colour of fishing for acquisition at wild environment is all silver-gray individuality mostly, does not still report yellow individual appearance.This is because under natural water area environment, large squama Barb breeds parental population comparatively greatly, and genetic diversity is higher, and the possibility of the filial generation inbreeding of generation is less, even if there is the individuality of recessive body colour to occur, also can ingest by natural selection or by other feral animals.
Summary of the invention
The invention provides a kind of producing method for seed being sheerly yellow large squama Barb, providing operation basis for obtaining the yellow large squama Barb of pure lines.
A kind of producing method for seed being sheerly yellow large squama Barb of the present invention, it carries out according to following steps:
One, adopt from natural water area and catch the large squama Barb of wild silver gray, cultivate to gonad maturity;
Two, female, the male wild large squama Barb of previous step gonad maturity is carried out artificial induced spawning, insemination and hatching, obtain F1 silver gray individual; The F1 silver gray individuality obtained is carried out next-step operation;
Three, the silver gray F1 individual feeding upper step obtained, in pond, is cultivated to gonad maturity;
Four, take the filial generation inbreeding of same Parent, female for the F1 of previous step gonad maturity, milter are carried out artificial induced spawning, insemination and hatching, bred by silver gray F1 selfing and obtain F2;
Five, F2 reaches after 2 ~ 3cm through pond rearing, and it is individual that separation screening obtains yellow large squama Barb;
The large squama Barb individuality of the yellow six, obtained by previous step separation screening retains, then cultivates to gonad maturity;
Seven, cultivate to sexually matured yellow large squama Barb individuality in previous step, female, the male parent population of Stochastic choice carries out artificial induced spawning, insemination and hatching;
Eight, the filial generation obtained by previous step is all that yellow large squama Barb is individual, namely completes the production of hybrid seeds process of the yellow large squama Barb of described pure lines.
The present invention comprises following beneficial effect:
The present invention catches the large squama Barb of 1 wild silver gray in age breed by adopting natural water area, finally cultivates the fingerling completely with the yellow large squama Barb of pure lines.
Large quantity research shows, cinerous and the Red color trait of most fish are controlled by 2 pairs of genes, cinerous is dominant, red is recessive, genotype is that RRBB, RrBB, rrBB, RRBb, RRbb, RrBb, rrBb and Rrbb show dominant steel gray body colour, only has genotype to be that rrbb just can show redness.Therefore, occur that the large squama Barb probability of recessive body colour is less at occurring in nature, even if there is the individuality of other body colour recessive to occur, also can ingest by natural selection or by other feral animals.
A kind of producing method for seed being sheerly yellow large squama Barb that the present invention adopts, first the father and mother parent with recessive gene r and b is obtained by the mating of wild large squama Barb, but because the genotype showing steel gray body colour is more, therefore, yellow can't be there is in most of silver gray F1 self-bred progeny, only have Parent to control two pairs of genes of body colour simultaneously all with recessive gene, if genotype is RrBb, rrBb and Rrbb mating, just may there is yellow in filial generation.According to Mendelian independent assortment genetic development, RrBb × RrBb mating, there will be the genotype rrbb yellow of 1/16; RrBb × rrBb or Rrbb mating, there will be the genotype rrbb yellow of 1/8; RrBb × Rrbb mating, there will be the genotype rrbb yellow of 1/4.Due to the homozygote that yellow is recessive genotype rrbb, therefore, the filial generation of yellow F2 selfing is all yellow, namely obtains a kind of producing method for seed being sheerly yellow large squama Barb.
Accompanying drawing explanation
Fig. 1 is the yellow large squama Barb seed selection flow chart of the present invention.
Embodiment
Embodiment one: a kind of producing method for seed being sheerly yellow large squama Barb of present embodiment, it carries out according to following steps:
One, adopt from natural water area and catch the large squama Barb of wild silver gray, cultivate 3 ~ 4 years to gonad maturity;
Two, female, the male wild large squama Barb of previous step gonad maturity is carried out artificial induced spawning, insemination and hatching, obtain F1 silver gray individual; The F1 silver gray individuality obtained is carried out next-step operation;
Three, the silver gray F1 individual feeding upper step obtained, in pond, cultivates 4 ~ 5 years to gonad maturity;
Four, take the filial generation inbreeding of same Parent, female for the F1 of previous step gonad maturity, milter are carried out artificial induced spawning, insemination and hatching, bred by silver gray F1 selfing and obtain F2;
Five, F2 reaches after 2 ~ 3cm through pond rearing, and it is individual that separation screening obtains yellow large squama Barb;
The large squama Barb individuality of the yellow six, obtained by previous step separation screening retains, then cultivates 4 ~ 5 years to gonad maturity;
Seven, cultivate to sexually matured yellow large squama Barb individuality in previous step, female, the male parent population of Stochastic choice carries out artificial induced spawning, insemination and hatching;
Eight, the filial generation obtained by previous step is all that yellow large squama Barb is individual, namely completes the production of hybrid seeds process of the yellow large squama Barb of described pure lines.
Embodiment two: present embodiment and embodiment one unlike: the large squama Barb of wild silver gray introduces from Uzbekistan.Other is identical with embodiment one.
Content of the present invention is not limited only to the content of the respective embodiments described above, and the combination of one of them or several embodiment equally also can realize the object of inventing.
Beneficial effect of the present invention is verified by following examples:
A kind of producing method for seed being sheerly yellow large squama Barb of the present embodiment, it carries out according to following steps:
The first step: adopt from natural water area and catch the large squama Barb of 1 wild silver gray in age, cultivate 3 ~ 4 years to gonad maturity;
Second step: carry out 6 ~ 8 group of 1 female artificial induced spawning to 3 male wild large squama Barb, insemination and hatching, the F1 of acquisition is all that silver gray is individual;
3rd step: raised respectively in pond by the silver gray F1 often organizing generation, cultivates 4 ~ 5 years to gonad maturity;
4th step: the fish inbreeding taking same pond, every group selection 5 ~ 8 carries out artificial induced spawning, insemination and hatching to female milter, is bred obtain F2 by silver gray F1 selfing;
5th step: F2 reaches 2 ~ 3cm through the pond rearing of 20 ~ 25d, observes and finds in the filial generation of 1 combination, is separated and has yellow recessiveness individual.Through statistics in 2014, different body colour segregation ratio was yellow 15.6%, silver gray 84.4%;
6th step: the individuality of yellow is retained, then cultivate 4 ~ 5 years to gonad maturity;
7th step: in the sexually matured yellow individuality of previous step, Stochastic choice 15 pairs of male and female parent populations carry out artificial induced spawning, naturally inseminate;
8th step: what observe the filial generation 100% of previous step acquisition is yellow individual, proves to obtain the large squama Barb of yellow of pure lines.
Interpretation of result:
The seed selection situation of the large squama Barb of Yellow strain of the present embodiment is shown in Table 1;
The seed selection of the large squama Barb of table 1 Yellow strain
When large squama Barb fry only has cultivation to 2 ~ 3cm, could better distinguish is silver-gray and yellow cultivars.As shown in Table 1, the experiment initial stage is all put together by the F2 fry of 5 ~ 6 groups to raise together with during 2012 ~ 2013, and observe in the filial generation finding different pond rearing, some combinations can produce yellow cultivars, and some combinations do not have yellow cultivars.Within 2014, add up one and occurred that the filial generation body colour separation case in more yellow large squama Barb fishpond is as table 2:
The segregation ratio of yellow large squama Barb F2 is had in table 22014 year filial generation
As shown in Figure 1, the method for the present embodiment, successfully cultivates yellow fish products kind.

Claims (2)

1. be sheerly a producing method for seed of yellow large squama Barb, it is characterized in that it carries out according to following steps:
One, adopt from natural water area and catch the large squama Barb of wild silver gray, cultivate to gonad maturity;
Two, female, the male wild large squama Barb of previous step gonad maturity is carried out artificial induced spawning, insemination and hatching, obtain F1 silver gray individual; The F1 silver gray individuality obtained is carried out next-step operation;
Three, the silver gray F1 individual feeding upper step obtained, in pond, is cultivated to gonad maturity;
Four, take the filial generation inbreeding of same Parent, female for the F1 of previous step gonad maturity, milter are carried out artificial induced spawning, insemination and hatching, bred by silver gray F1 selfing and obtain F2;
Five, F2 reaches after 2 ~ 3cm through pond rearing, and it is individual that separation screening obtains yellow large squama Barb;
The large squama Barb individuality of the yellow six, obtained by previous step separation screening retains, then cultivates to gonad maturity;
Seven, cultivate to sexually matured yellow large squama Barb individuality in previous step, female, the male parent population of Stochastic choice carries out artificial induced spawning, insemination and hatching;
Eight, the filial generation obtained by previous step is all that yellow large squama Barb is individual, namely completes the production of hybrid seeds process of the yellow large squama Barb of described pure lines.
2. a kind of producing method for seed being sheerly yellow large squama Barb according to claim 1, is characterized in that the large squama Barb of wild silver gray introduces from Uzbekistan.
CN201510962779.XA 2015-12-18 2015-12-18 A kind of producing method for seed for being sheerly the big squama Barb of yellow Expired - Fee Related CN105409857B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109744170A (en) * 2019-03-13 2019-05-14 沈阳华泰渔业有限公司 A kind of producing method for seed being sheerly the paradise fish of China
CN110692550A (en) * 2019-08-06 2020-01-17 山东省淡水渔业研究院 Method for producing seeds of golden yellow snakeheads
CN112741029A (en) * 2021-02-04 2021-05-04 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp
CN112889711A (en) * 2021-01-15 2021-06-04 中国水产科学研究院黑龙江水产研究所 Artificial breeding method of yellow large-scale barbel

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* Cited by examiner, † Cited by third party
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张建森 等: "鲤鱼体色体型遗传的研究", 《水产学报》 *
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李中科 等: "大鳞鲃人工繁育试验报告", 《河北渔业》 *
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109744170A (en) * 2019-03-13 2019-05-14 沈阳华泰渔业有限公司 A kind of producing method for seed being sheerly the paradise fish of China
CN110692550A (en) * 2019-08-06 2020-01-17 山东省淡水渔业研究院 Method for producing seeds of golden yellow snakeheads
CN112889711A (en) * 2021-01-15 2021-06-04 中国水产科学研究院黑龙江水产研究所 Artificial breeding method of yellow large-scale barbel
CN112889711B (en) * 2021-01-15 2021-11-16 中国水产科学研究院黑龙江水产研究所 Artificial breeding method of yellow large-scale barbel
CN112741029A (en) * 2021-02-04 2021-05-04 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp
CN112741029B (en) * 2021-02-04 2022-06-07 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp

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