CN112889711A - Artificial breeding method of yellow large-scale barbel - Google Patents
Artificial breeding method of yellow large-scale barbel Download PDFInfo
- Publication number
- CN112889711A CN112889711A CN202110053645.1A CN202110053645A CN112889711A CN 112889711 A CN112889711 A CN 112889711A CN 202110053645 A CN202110053645 A CN 202110053645A CN 112889711 A CN112889711 A CN 112889711A
- Authority
- CN
- China
- Prior art keywords
- pond
- water
- spawning
- fish
- loop
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 38
- 241000124815 Barbus barbus Species 0.000 title abstract description 15
- 210000000084 barbel Anatomy 0.000 title abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 95
- 241000251468 Actinopterygii Species 0.000 claims abstract description 92
- 230000001488 breeding effect Effects 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 16
- 230000012447 hatching Effects 0.000 claims abstract description 15
- 239000003814 drug Substances 0.000 claims abstract description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 13
- 239000001301 oxygen Substances 0.000 claims abstract description 13
- 230000006698 induction Effects 0.000 claims abstract description 10
- 230000004720 fertilization Effects 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims abstract description 6
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 6
- 230000001939 inductive effect Effects 0.000 claims abstract description 5
- 241000132906 Tubificidae Species 0.000 claims abstract description 4
- 235000013601 eggs Nutrition 0.000 claims description 20
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 230000002196 ecbolic effect Effects 0.000 claims description 13
- 238000005276 aerator Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 12
- 241001302443 Luciobarbus capito Species 0.000 claims description 10
- 210000002969 egg yolk Anatomy 0.000 claims description 10
- 230000003203 everyday effect Effects 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 8
- 108010000912 Egg Proteins Proteins 0.000 claims description 8
- 235000013345 egg yolk Nutrition 0.000 claims description 8
- 241001465754 Metazoa Species 0.000 claims description 7
- 229940079593 drug Drugs 0.000 claims description 7
- 230000002354 daily effect Effects 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 241000306814 Capito Species 0.000 claims description 5
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 4
- 239000013505 freshwater Substances 0.000 claims description 4
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 4
- 230000000384 rearing effect Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000010255 intramuscular injection Methods 0.000 claims description 3
- 239000007927 intramuscular injection Substances 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 241001604129 Polydactylus Species 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 4
- 230000026109 gonad development Effects 0.000 abstract description 4
- 230000001502 supplementing effect Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 7
- 241001275872 Sinocyclocheilus grahami Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 210000002149 gonad Anatomy 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 241000252210 Cyprinidae Species 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- QLGKMLRGKPKGKI-QWQCQAQZSA-N (2s)-n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl Chemical compound CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H]1NC(=O)CC1)CC1=CC=C(O)C=C1 QLGKMLRGKPKGKI-QWQCQAQZSA-N 0.000 description 1
- CPKVUHPKYQGHMW-UHFFFAOYSA-N 1-ethenylpyrrolidin-2-one;molecular iodine Chemical compound II.C=CN1CCCC1=O CPKVUHPKYQGHMW-UHFFFAOYSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000732800 Cymbidium Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001460311 Luciobarbus brachycephalus Species 0.000 description 1
- 241001098054 Pollachius pollachius Species 0.000 description 1
- 229920000153 Povidone-iodine Polymers 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- DPWSRXJWCYEGIV-CWMZTGDLSA-N acetic acid;n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxoprop Chemical compound CC(O)=O.CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 DPWSRXJWCYEGIV-CWMZTGDLSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 241001233061 earthworms Species 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229960001952 metrifonate Drugs 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229960001621 povidone-iodine Drugs 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- NFACJZMKEDPNKN-UHFFFAOYSA-N trichlorfon Chemical compound COP(=O)(OC)C(O)C(Cl)(Cl)Cl NFACJZMKEDPNKN-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
An artificial breeding method of yellow large-scale barbel, belongs to the technical field of aquaculture, and relates to an artificial breeding method of yellow large-scale barbel. The invention aims to solve the problems that an artificial breeding technology suitable for yellow large-scale barbel does not exist at present, and the artificial induced spawning rate, the fertilization rate and the seedling survival rate are low. The breeding process comprises five links of parent fish breeding, parent fish spawning induction, natural spawning fertilization, fertilized egg circular loop hatching and fry breeding. The gonad development and maturity of the parent fish are strengthened by singly culturing and supplementing the bait for the tubificidae animals. Optimizing the dosage of the artificial spawning-inducing medicine suitable for the natural spawning of the parent fish. Utilizes the stimulation of the circular water flow to improve the natural spawning rate of the parent fish. The flow rate of water flow in the loop and the using time of external circulation and internal circulation are adjusted, and the hatchability reaches 72.12 percent. The survival rate of the fry pond reaches 65.33 percent by adopting a feeding strategy of controlling dissolved oxygen, bacillus and photosynthetic bacteria in the fry pond to adjust water quality and gradually transitioning from natural bait to artificial bait.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to an artificial breeding method of yellow sinocyclocheilus grahami.
Background
Barbus capito (Luciobarbus brachycephalus) belongs to Cyprinidae, Cyprinidae and Barcheideidae, is native to the waters of the south of the saltwater, the Lihai and the West, and is introduced into China from the Sulzbekkan saltwater of the Heilongjiang aquatic research institute in 2003, and the product name of the Barbus capito is fresh water pollack. The invention patent of 'a seed production method of pure line yellow large-scale barbel' in 09.01.8.2018, with the authorization number of CN105409857B, is characterized in that the wild silver gray large-scale barbel is captured from a natural water area and cultivated until the gonad is mature, and the yellow large-scale barbel individual is finally obtained through hybridization and breeding. The patent discloses a germplasm source of yellow sinocyclocheilus grahami, and does not relate to an artificial breeding method. The yellow large-scale barbel is a homozygote of a yellow recessive genotype, and has the characteristics of orange body color, hypoxia tolerance, easy domestication, low seedling survival rate and the like. No literature is reported about the successful artificial breeding of the yellow large-scale barbel, and the breeding method of the carpi or the silver gray large-scale barbel is basically used for reference. The breeding effect of the current yellow large-scale barbel is not ideal, and the artificial induced spawning rate, the fertilization rate and the seedling survival rate are all less than 50%.
Disclosure of Invention
The invention aims to solve the problems that an artificial breeding technology suitable for yellow large-scale barbel does not exist at present, and the artificial yield rate, the fertilization rate and the seedling survival rate are low, and provides an artificial breeding method for the yellow large-scale barbel.
The artificial breeding method of the yellow Barcheilus capito comprises the following steps of:
firstly, parent fish cultivation:
the parent fish breeding adopts a pond single breeding mode, the stocking density of parent fish is 200 tails/mu, the artificial compound pellet feed is fed to the parent fish in fresh water, the daily bait feeding amount is 3-5% of the weight of the fish, and the animal bait tubificidae is fed for 1 time every day 2 months before artificial propagation;
secondly, spawning induction of parent fishes:
in the breeding season of 5-6 months in spring, selecting mature parent fish according to the ratio of male and female 1:1.5-1:2, and artificially inducing spawning, wherein the dosage of the female fish induced spawning medicine is LHRH-A210 mug/kg, HCG 150IU/kg, DOM 2mg/kg, the male fish dosage is halved;
thirdly, natural spawning and fertilization:
the spawning pond and the fertilized egg hatching pond are in the same circular loop, the radius of an inner loop of the circular loop is 2.0m, the radius of an outer loop is 2.7m, the width of the loop is 0.7m, the depth of water in the loop is 1m, the external circulation and the internal circulation can be realized, the external circulation is that the water flow in the loop rotates under the pressure of an external water source, the internal circulation is that no external water source is supplied, the water flow in the loop rotates under the pressure of a water pump, the water temperature is 22-25 ℃, the flow rate is 0.25-0.30m/s, the parent fish injected with the oxytocic is put into the spawning pond according to the proportion of 1:1.5-1:2, the parent fish naturally spawns and fertilizes in the internal circulation mode, the parent fish is transferred to the pond after spawning is finished, the postpartum culture is started, and;
fourthly, hatching fertilized eggs in a circular path:
hatching fertilized eggs in a circular loop by adopting combination of external circulation and internal circulation, sterilizing twice with 20ppm methylene blue every day for 30min each time, preventing and controlling saprolegniasis, controlling the flow velocity of water in the loop to be 0.17-0.21m/s and the water temperature to be 22-25 ℃;
fifthly, fry rearing:
opening the incubated fry after 5-6 days of incubation, transferring the fry to an outdoor pond for incubation, preparing plankton in the pond in advance to reach a peak period, measuring dissolved oxygen in water before the fry is put into the pond, aerating the fry with an aerator to reduce the dissolved oxygen to below 7mg/L after the dissolved oxygen exceeds 9mg/L, putting the fry into the pond, starting the waterwheel aerator in sunny days for 4-6h after putting the fry into the pond, starting the waterwheel aerator in cloudy days for 4-5h at night, controlling the dissolved oxygen in water to be 6-8mg/L, adjusting water quality with bacillus and photosynthetic bacteria every 5 days, feeding egg yolk water two days before the fry is put into the pond, alternately feeding egg yolk and the fry opening material from a wind head of the pond after balancing water temperature, starting to feed egg yolk and the fry opening material on the 4 th day after putting the pond, feeding 0.3mm to 10 mm of broken material every 3 times every day, wherein the total length reaches 2.5-3cm after 20 days, the fish can be transferred to a culture pond for fish culture.
The oxytocic dosage of the invention is several times lower than the existing dosage, and the beneficial effects are that the cost of the oxytocic can be reduced; and secondly, the drug resistance of the fish to the drugs is reduced. The parent fish before induced spawning can promote the gonad development and maturation to be improved by 20-30% by supplementing animal baits.
The gonads of the parent fishes mature by singly culturing and supplementing the bait of the tubificidae animals. Optimizing the dosage of artificial spawning-inducing medicine (LHRH-A) suitable for parent fish to spawn naturally2(10. mu.g/kg) + HCG (150IU/kg) + DOM (2 mg/kg)). The parent fish is not needed to be anesthetized by drugs when injected, so that the influence of the anesthetic on the fish body is avoided. The natural spawning rate of parent fish reaches 73.33 percent by utilizing the stimulation of the circular water flow, thereby avoiding the difficulty of accurate control and repeated check of egg squeezing when the eggs are squeezed manuallyThe parent fish is easy to be injured and killed. The flow rate of water for hatching in the loop is controlled to be 0.17-0.21m/s, the hatching is carried out by combining external circulation and internal circulation, and the hatching rate reaches 72.12 percent. By adopting a feeding strategy of controlling dissolved oxygen in the pond, regulating water quality by bacillus and photosynthetic bacteria and gradually transiting artificial bait by natural bait, the survival rate of the fry pond reaches 65.33 percent. The invention successfully establishes the artificial breeding technology of the yellow large-scale barbel.
Drawings
FIG. 1 is a photograph of a circular loop used in the present invention.
Detailed Description
The technical solution of the present invention is not limited to the following specific embodiments, but includes any combination of the specific embodiments.
The first embodiment is as follows: the artificial breeding method of the yellow sinocyclocheilus grahami is carried out according to the following steps:
firstly, parent fish cultivation:
the parent fish culture adopts a pond single-culture mode, the stocking density of parent fish is 200 tails/mu, the artificial compound granular feed for fresh water parent fish is fed, the daily bait feeding amount is 3-5% of the weight of the fish, and the zooglomus gigas is fed for 1 time every day 2 months before artificial propagation (compared with the parent fish which is not fed with animal bait before induction, the gonad development maturity can be improved by 20-30%);
secondly, spawning induction of parent fishes:
in the breeding season of 5-6 months in spring, selecting mature parent fish according to the ratio of male and female 1:1.5-1:2, and artificially inducing spawning, wherein the dosage of the female fish induced spawning medicine is LHRH-A210 mug/kg, HCG 150IU/kg, DOM 2mg/kg, the male fish dosage is halved;
thirdly, natural spawning and fertilization:
the spawning pond and the fertilized egg hatching pond are in the same circular loop, the radius of an inner loop of the circular loop is 2.0m, the radius of an outer loop is 2.7m, the width of the loop is 0.7m, the depth of water in the loop is 1m, the external circulation and the internal circulation can be realized, the external circulation is that the water flow in the loop rotates under the pressure of an external water source, the internal circulation is that no external water source is supplied, the water flow in the loop rotates under the pressure of a water pump, the water temperature is 22-25 ℃, the flow rate is 0.25-0.30m/s, the parent fish injected with the oxytocic is put into the spawning pond according to the proportion of 1:1.5-1:2, the parent fish naturally spawns and fertilizes in the internal circulation mode, the parent fish is transferred to the pond after spawning is finished, the postpartum culture is started, and;
fourthly, hatching fertilized eggs in a circular path:
hatching fertilized eggs in a circular loop by adopting combination of external circulation and internal circulation, sterilizing twice with 20ppm methylene blue every day for 30min each time, preventing and controlling saprolegniasis, controlling the flow velocity of water in the loop to be 0.17-0.21m/s and the water temperature to be 22-25 ℃;
fifthly, fry rearing:
after 5-6 days of incubation, the fries are opened horizontally and transferred to an outdoor pond for incubation, the pond is disinfected at the bottom of the pond in the early period, new water is added and fertilizer application is prepared, natural biological bait rotifers of the fries reach the peak period (5000 to 6000 fries per liter), rich opening bait is provided for the fries, dissolved oxygen in water is measured before the fries are put into the pond, the fries are aerated by an aerator and are reduced to below 7mg per liter, then the fries are put into the pond, a water vehicle aerator is started for 4-6 hours in sunny days after the fries are put into the pond, the water vehicle aerator is started for 4-5 hours in cloudy days, dissolved oxygen in water is controlled to be 6-8 mg/liter, bacillus and photosynthetic bacteria are used every 5 days to keep fat, tender and tasty water, egg yolk water is fed before the fries are put into the pond for two days, the fries are put into the pond from deep water of a wind head after water temperature is balanced, and alternately feeding yolk and larval fish starter on the 4 th day after the fish is put into the pond, feeding the crushed materials with the length of 0.3mm 3-4 times a day, feeding the crushed materials with the length of 0.3mm in 9-10 days 4 times a day, and transferring the crushed materials to a culture pond to culture the fish seeds after the total length of the crushed materials reaches 2.5-3cm in about 20 days.
The second embodiment is as follows: the difference between the first embodiment and the second embodiment is that in the first step, the pond is selected to be 3-6 mu, the water source is pollution-free, the water inlet and the water outlet are convenient, the sunlight is sufficient, the traffic is convenient, and the electric power matching condition is good. The rest is the same as the first embodiment.
The third concrete implementation mode: the difference between the first and second embodiments is that in the second step, a one-time injection method is adopted, the injection method is intramuscular injection at the base of dorsal fins, and during injection, the parent fish is not anesthetized by medicines, and the fish body is slightly supported until the base of dorsal fins is exposed out of the water surface for injection. The others are the same as in the first or second embodiment.
The fourth concrete implementation mode: the difference between this embodiment and one of the first to third embodiments is that the daily bait dosage in the first step is 4% of the fish weight. The rest is the same as one of the first to third embodiments.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is that in the third step, the parent fish injected with the oxytocic is placed in the spawning pond at a ratio of male and female 1:1.5 under the conditions that the water temperature is 22 ℃ and the flow rate is 0.26 m/s. The rest is the same as one of the first to fourth embodiments.
The sixth specific implementation mode: the difference between this embodiment and the first to the fifth embodiment is that in the third step, the parent fish injected with the oxytocic is placed in the spawning pond at a ratio of male and female 1:1.6 under the conditions that the water temperature is 23 ℃ and the flow rate is 0.27 m/s. The rest is the same as one of the first to fifth embodiments.
The seventh embodiment: the difference between this embodiment and the first to sixth embodiments is that in the third step, the parent fish injected with the oxytocic is placed in the spawning pond at a ratio of male and female 1:1.7 under the conditions of water temperature of 24 ℃ and flow rate of 0.28 m/s. The rest is the same as one of the first to sixth embodiments.
The specific implementation mode is eight: the difference between this embodiment and the first to seventh embodiments is that in the fourth step, the flow rate of the water in the loop is controlled to be 0.18m/s, and the water temperature is controlled to be 22 ℃. The rest is the same as one of the first to seventh embodiments.
The specific implementation method nine: the difference between this embodiment and the first to eighth embodiments is that in the fourth step, the flow rate of the water in the loop is controlled to be 0.19m/s, and the water temperature is controlled to be 23 ℃. The rest is the same as the first to eighth embodiments.
The detailed implementation mode is ten: the difference between this embodiment and one of the first to ninth embodiments is that in the fourth step, the flow rate of the water in the loop is controlled to be 0.20m/s, and the water temperature is controlled to be 24 ℃. The rest is the same as one of the first to ninth embodiments.
The following experiments are adopted to verify the effect of the invention:
experiment one:
the yellow Barbus capito artificial breeding method comprises the following steps:
firstly, parent fish cultivation:
3 mu of pond B61 is selected as a parent fish breeding pond at the Holland aquatic product test station of the research institute of Heilongjiang aquatic products. The pond is in the shape of a rectangle with east-west length, south-north width and the length-width ratio of 2: 1. Adding river water of cymbidium at a depth of 1.5 m for 4 months and 7 days, and sterilizing with 0.7ppm crystal trichlorfon, 0.5ppm copper sulfate, and 0.2ppm povidone iodine. Transferring 600 tails of yellow parent fish of Barcheilus capito from the overwintering pond in 16 days 4 months, wherein the body length is 40.2-54.1cm, and the body weight is 0.90-2.23 kg. Sterilizing with 2% saline for 20min before being placed into pond. 3.5mm parent fish pellet feed produced by Harbin wetting agriculture and animal husbandry Limited company is fed, the daily feeding amount is 36kg according to 3 percent of the total weight of the fish, the feeding is carried out for 3 times, then the feeding is gradually increased to 5 percent, and the feeding is carried out for 4 times. Meanwhile, 20kg of the aquatic earthworms serving as the animal bait is fed every day (compared with parent fishes which are not supplemented with the animal bait before induction, the growth and maturity of gonads can be improved by 20-30%).
Secondly, spawning induction of parent fishes:
6 months and 4 days, the water temperature in the pond reaches 19 ℃, the gonad development maturity of parent fish is checked, female fish and male fish are selected for artificial spawning induction, and the spawning induction drug dosage is female fish LHRH-A2(10 μ g/kg) + HCG (150IU/kg) + DOM (2mg/kg), 2-2.5ml is injected according to the weight, and the dosage of the male fish is halved. The injection method is characterized in that a needle injection method is adopted, the injection method is intramuscular injection at the base of the dorsal fin, no drug is used for anesthesia during injection, and the injection is carried out in a white plastic box for transporting fish by slightly supporting the fish body until the base of the dorsal fin is exposed out of the water surface.
Thirdly, natural spawning and fertilization:
the parent fish 15-tailed female fish and 25-tailed male fish injected with oxytocic are put into the loop to naturally lay eggs. The water depth in the loop is 1m, the internal circulation is adopted during the spawning period of the parent fish, and the flow rate is controlled to be 0.25-0.30m/s by adjusting a water outlet valve. At the water temperature of 22 ℃, spawning begins 15 hours after the injection of the oxytocic, and the spawning peak is reached within 18-23 hours. After spawning, the parent fish is transferred to a pond for postpartum cultivation, 11 female 15 fishes successfully spawn, and the spawning induction rate is 73.33%. Fertilized eggs are left in the circular path for hatching.
Fourthly, hatching fertilized eggs in a circular path:
after the spawning is finished, the external circulation is used, and the water outlet valve is adjusted to control the flow rate of water flow in the loop to be 0.17-0.21 m/s. Sterilizing with 20ppm methylene blue twice a day for 30min each time, and preventing and controlling saprolegniasis. The internal circulation is used during the disinfection period to maintain the concentration of the medicine to be constant; after the disinfection is finished, the medicine concentration is gradually diluted to 0 by using external circulation and supplementing new water source. At the water temperature of 22 ℃, fertilized eggs are hatched for 52 hours to start rupture of membranes and emerge, and about 6 hours of total emergence. The internal circulation is used during the emergence of seedlings, and the external circulation is used after the emergence of seedlings.
Fifthly, fry rearing:
and 6, 13 days after 6 months, culturing the larval fish in the circular loop for 6 days, starting floating and level swimming, and timely transferring the larval fish to an outdoor pond for culturing. And 3 mu of pond B63 is selected according to the number of the fries. The pond is prepared in the early stage, the water depth is 0.8m, plankton such as rotifers reaches the peak, and the pond is natural and high-quality initial feed for the fries. The fry is put in the pond at 17:00 evening in sunny days, the pond water is aerated for 5 hours by a 1.5kw waterwheel aerator, and the dissolved oxygen is 6.8 mg/L. And collecting the fry in the circular path by using a fry collector, and counting about 75 ten thousand fries. Egg yolk water is fed before the fry are put into the pond, and the fry are put into the pond from the deep water position of the wind head on the pond after the water temperature is balanced. After the pond is placed, the aerator is started at 10:00-15:00 in the morning on a sunny day, and the aerator is started at 23:00-4:00 in the evening on a cloudy day. And (3) regulating the water quality by using the bacillus and the photosynthetic bacteria every 5 days, and timely filling well water to keep the water quality fresh. And alternately feeding the yolk water and the seed opening material on the 4 th day after the egg is placed in the pond, and 3-4 times per day. Feeding 0.3mm crushed material at 23 days after 6 months, and feeding 2-4kg each time according to the feeding condition of fry, 4 times per day. The total length of the fry reaches 2.5-3cm in the beginning of 7 months, and the fry can be transferred to a culture pond for fish culture.
The natural spawning and artificial egg squeezing modes in the experiment are as follows:
TABLE 1 comparison of reproductive Performance of Natural spawning and Artificial egg extrusion
It is shown in Table 1 that the natural spawning hastening rate and the fertilization rate are higher than those of the artificial egg extrusion, and the artificial egg extrusion is safe and reliable and cannot cause casualty.
The action of the circular loop internal circulation and the circular loop external circulation in the invention is as follows:
TABLE 2 comparison of the effects of internal and external circulation in a circular circuit
Claims (10)
1. The artificial breeding method of the yellow Barbus capito is characterized in that the artificial breeding method of the yellow Barbus capito is carried out according to the following steps:
firstly, parent fish cultivation:
the parent fish breeding adopts a pond single breeding mode, the stocking density of parent fish is 200 tails/mu, the artificial compound pellet feed is fed to the parent fish in fresh water, the daily bait feeding amount is 3-5% of the weight of the fish, and the animal bait tubificidae is fed for 1 time every day 2 months before artificial propagation;
secondly, spawning induction of parent fishes:
in the breeding season of 5-6 months in spring, selecting mature parent fish according to the ratio of male and female 1:1.5-1:2, and artificially inducing spawning, wherein the dosage of the female fish induced spawning medicine is LHRH-A210 mug/kg, HCG 150IU/kg, DOM 2mg/kg, the male fish dosage is halved;
thirdly, natural spawning and fertilization:
the spawning pond and the fertilized egg hatching pond are in the same circular loop, the radius of an inner loop of the circular loop is 2.0m, the radius of an outer loop is 2.7m, the width of the loop is 0.7m, the depth of water in the loop is 1m, the external circulation and the internal circulation can be realized, the external circulation is that the water flow in the loop rotates under the pressure of an external water source, the internal circulation is that no external water source is supplied, the water flow in the loop rotates under the pressure of a water pump, the water temperature is 22-25 ℃, the flow rate is 0.25-0.30m/s, the parent fish injected with the oxytocic is put into the spawning pond according to the proportion of 1:1.5-1:2, the parent fish naturally spawns and fertilizes in the internal circulation mode, the parent fish is transferred to the pond after spawning is finished, the postpartum culture is started, and;
fourthly, hatching fertilized eggs in a circular path:
hatching fertilized eggs in a circular loop by adopting combination of external circulation and internal circulation, sterilizing twice with 20ppm methylene blue every day for 30min each time, preventing and controlling saprolegniasis, controlling the flow velocity of water in the loop to be 0.17-0.21m/s and the water temperature to be 22-25 ℃;
fifthly, fry rearing:
opening the incubated fry after 5-6 days of incubation, transferring the fry to an outdoor pond for incubation, preparing plankton in the pond in advance to reach a peak period, measuring dissolved oxygen in water before the fry is put into the pond, aerating the fry with an aerator to reduce the dissolved oxygen to below 7mg/L after the dissolved oxygen exceeds 9mg/L, putting the fry into the pond, starting the waterwheel aerator in sunny days for 4-6h after putting the fry into the pond, starting the waterwheel aerator in cloudy days for 4-5h at night, controlling the dissolved oxygen in water to be 6-8mg/L, adjusting water quality with bacillus and photosynthetic bacteria every 5 days, feeding egg yolk water two days before the fry is put into the pond, alternately feeding egg yolk and the fry opening material from a wind head of the pond after balancing water temperature, starting to feed egg yolk and the fry opening material on the 4 th day after putting the pond, feeding 0.3mm to 10 mm of broken material every 3 times every day, wherein the total length reaches 2.5-3cm after 20 days, the fish can be transferred to a culture pond for fish culture.
2. The artificial breeding method of yellow barbus dabryi according to claim 1, wherein in the first step, a pond with the size of 3-6 mu, no pollution to water source, convenient water intake and drainage, sufficient sunlight, convenient traffic and good power matching conditions is selected.
3. The artificial breeding method of yellow barbus capito according to claim 1, wherein in the second step, a one-shot injection method is adopted, the injection method is intramuscular injection at the base of dorsal fin, and during the injection, the parent fish is not anesthetized by drugs, and the injection is performed by slightly supporting the fish body until the base of dorsal fin is exposed out of the water surface.
4. The method for artificially breeding the yellow barbus capito according to claim 1, wherein the daily bait amount in the first step is 4% of the weight of the fish.
5. The method for artificially breeding the yellow Barcheilus capito according to claim 1, wherein the parent fish injected with the oxytocic drug is placed in a spawning pond according to the ratio of male to female 1:1.5 under the conditions that the water temperature is 22 ℃ and the flow rate is 0.26m/s in the third step.
6. The method for artificially breeding the yellow Barcheilus capito according to claim 1, wherein the parent fish injected with the oxytocic is placed in a spawning pond according to the ratio of male to female 1:1.6 under the conditions that the water temperature is 23 ℃ and the flow rate is 0.27m/s in the third step.
7. The method for artificially breeding the yellow Barcheilus capito according to claim 1, wherein the parent fish injected with the oxytocic is placed in a spawning pond according to the ratio of male to female 1:1.7 under the conditions that the water temperature is 24 ℃ and the flow rate is 0.28m/s in the third step.
8. The method for artificially breeding the yellow barbus capito according to claim 1, wherein the flow rate of water in the loop is controlled to be 0.18m/s in the fourth step, and the water temperature is controlled to be 22 ℃.
9. The method for artificially breeding the yellow barbus capito according to claim 1, wherein the flow rate of water in the loop is controlled to be 0.19m/s in the fourth step, and the water temperature is controlled to be 23 ℃.
10. The method for artificially breeding the yellow barbus capito according to claim 1, wherein the flow rate of water in the loop is controlled to be 0.20m/s in the fourth step, and the water temperature is controlled to be 24 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110053645.1A CN112889711B (en) | 2021-01-15 | 2021-01-15 | Artificial breeding method of yellow large-scale barbel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110053645.1A CN112889711B (en) | 2021-01-15 | 2021-01-15 | Artificial breeding method of yellow large-scale barbel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112889711A true CN112889711A (en) | 2021-06-04 |
| CN112889711B CN112889711B (en) | 2021-11-16 |
Family
ID=76114470
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110053645.1A Active CN112889711B (en) | 2021-01-15 | 2021-01-15 | Artificial breeding method of yellow large-scale barbel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112889711B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615609A (en) * | 2021-09-16 | 2021-11-09 | 四川洪光农业开发有限责任公司 | Breeding method suitable for carp fries and four major Chinese carps |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106280A (en) * | 2011-01-19 | 2011-06-29 | 新宾满族自治县水产技术推广站 | Odontobutis yaluensis breeding method |
| CN103931534A (en) * | 2014-05-05 | 2014-07-23 | 天津市天祥水产有限责任公司 | Barbus capito pond net cage efficient breeding method |
| CN104170778A (en) * | 2014-08-18 | 2014-12-03 | 永丰一帆水产科技有限公司 | Large-scale common sucker breeding method capable of controlling temperature of circulating water |
| CN104396818A (en) * | 2014-11-10 | 2015-03-11 | 江苏省农业科学院宿迁农科所 | Cultivation method of intercropping of barbus capito and loach |
| CN105409857A (en) * | 2015-12-18 | 2016-03-23 | 中国水产科学研究院黑龙江水产研究所 | Fingerling-preparing method for pure-line yellow barbus capito |
| CN105994100A (en) * | 2016-06-15 | 2016-10-12 | 浙江省海洋水产研究所 | Marine fish fry breeding device and method |
| CN106900608A (en) * | 2017-05-03 | 2017-06-30 | 重庆两江生态渔业发展有限公司 | A kind of breeding method of big squama Barb fishes |
| KR20170104014A (en) * | 2016-03-03 | 2017-09-14 | 전라남도 | Method for mass producing artificial seed of Amphiproion ephippium |
| CN108633792A (en) * | 2018-06-22 | 2018-10-12 | 中国水产科学研究院黑龙江水产研究所 | A kind of salt-soda soil fishing-agriculture collective species cultural method |
| CN110279044A (en) * | 2019-07-24 | 2019-09-27 | 天津农学院 | A kind of mixed feed modifying agent and its application for improving big squama Barb meat flavor |
| CN111316936A (en) * | 2018-12-13 | 2020-06-23 | 射阳县周正海家庭农场 | Static water stripping artificial propagation method for large-scale barbel fish |
-
2021
- 2021-01-15 CN CN202110053645.1A patent/CN112889711B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106280A (en) * | 2011-01-19 | 2011-06-29 | 新宾满族自治县水产技术推广站 | Odontobutis yaluensis breeding method |
| CN103931534A (en) * | 2014-05-05 | 2014-07-23 | 天津市天祥水产有限责任公司 | Barbus capito pond net cage efficient breeding method |
| CN104170778A (en) * | 2014-08-18 | 2014-12-03 | 永丰一帆水产科技有限公司 | Large-scale common sucker breeding method capable of controlling temperature of circulating water |
| CN104396818A (en) * | 2014-11-10 | 2015-03-11 | 江苏省农业科学院宿迁农科所 | Cultivation method of intercropping of barbus capito and loach |
| CN105409857A (en) * | 2015-12-18 | 2016-03-23 | 中国水产科学研究院黑龙江水产研究所 | Fingerling-preparing method for pure-line yellow barbus capito |
| KR20170104014A (en) * | 2016-03-03 | 2017-09-14 | 전라남도 | Method for mass producing artificial seed of Amphiproion ephippium |
| CN105994100A (en) * | 2016-06-15 | 2016-10-12 | 浙江省海洋水产研究所 | Marine fish fry breeding device and method |
| CN106900608A (en) * | 2017-05-03 | 2017-06-30 | 重庆两江生态渔业发展有限公司 | A kind of breeding method of big squama Barb fishes |
| CN108633792A (en) * | 2018-06-22 | 2018-10-12 | 中国水产科学研究院黑龙江水产研究所 | A kind of salt-soda soil fishing-agriculture collective species cultural method |
| CN111316936A (en) * | 2018-12-13 | 2020-06-23 | 射阳县周正海家庭农场 | Static water stripping artificial propagation method for large-scale barbel fish |
| CN110279044A (en) * | 2019-07-24 | 2019-09-27 | 天津农学院 | A kind of mixed feed modifying agent and its application for improving big squama Barb meat flavor |
Non-Patent Citations (11)
| Title |
|---|
| 丁辰龙: "大鳞鲃的人工繁殖及体色变异分析", 《天津师范大学学报(自然科学版)》 * |
| 国家海水鱼产业技术研发中学: "《国家海水鱼产业技术体系年度报告 2017》", 31 December 2018, 中国海洋大学出版社 * |
| 张棋: "大鳞鲃人工繁殖技术及胚胎发育观察", 《湖北农业科学》 * |
| 徐伟: "大鳞鲃的人工繁殖、胚胎发育和耐盐碱测定", 《水产学报》 * |
| 戈贤平: "《新编淡水养殖技术手册》", 30 April 2007, 上海科学技术出版社 * |
| 王衍亮: "《可持续水产养殖-资源 环境 质量 2003水产科技论坛》", 31 May 2004, 海洋出版社 * |
| 王飞飞: "两种体色大鳞鲃鱼的肌肉营养成分分析与品质评价", 《营养学报》 * |
| 甘肃省农牧厅: "《养鱼技术读本》", 30 November 2014, 甘肃科学技术出版社 * |
| 蔡灵: "大鳞鲃亲鱼培育及人工繁殖技术", 《河北科学》 * |
| 蔺玉华: "大鳞鲃的引进、驯化、繁育及研发", 《吉林农业大学学报》 * |
| 袁隆平: "《淡水鱼高效养殖关键技术 彩插版》", 31 January 2006, 中国三峡出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615609A (en) * | 2021-09-16 | 2021-11-09 | 四川洪光农业开发有限责任公司 | Breeding method suitable for carp fries and four major Chinese carps |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112889711B (en) | 2021-11-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101715747B (en) | Method for promoting Yangtze River saury to naturally spawn | |
| CN103444600B (en) | Two-stage efficient rearing method for freshwater shrimp fry | |
| CN101669452A (en) | Mimic ecological propagation method for breeding parent fish of American hilsa herring | |
| CN101103706B (en) | Reproduction method for American reeves shad artificial cultivation parent fish | |
| CN102379254A (en) | Controllable synchronous brooding breeding method of cherax quadricarinatus | |
| CN110463627A (en) | A kind of annual artificial fecundation method of mandarin fish | |
| CN112042570A (en) | Method for efficiently cultivating and domesticating micropterus salmoides water bloom seedlings in pond greenhouse | |
| CN110024722A (en) | A kind of artificial fecundation method of scavenger | |
| CN108142328B (en) | Early propagation and rapid seedling raising method for small yellow croakers | |
| CN112889711B (en) | Artificial breeding method of yellow large-scale barbel | |
| CN102919186B (en) | Artificial breeding method for sillago sihama | |
| CN101933482A (en) | Method for north-south hybridization and reproduction regulation breeding of Lateolabrax | |
| CN110754405B (en) | Large-scale production method of artemia salina imago | |
| CN112544512A (en) | Method and equipment for hatching and breeding spiny eels | |
| CN116349624B (en) | Batch cultivation method for round-mouth copper fish fries | |
| CN109819912B (en) | Fish, shrimp and vegetable culture system and culture method | |
| CN115251010B (en) | Hybridization breeding method for female salmon and male wide fin | |
| CN113383733B (en) | Artificial cultivation method for young spiny plum juvenile fish | |
| CN112136728B (en) | Artificial breeding method for first-filial generation parent fish of Gymnocypris duringii in circulating water system | |
| CN104585094B (en) | A kind of artificial fecundation method of silver-colored silver xenocypris | |
| CN114128663A (en) | Thermo-optical regulation and control method for anti-season propagation of parent fish of takifugu rubripes and application of thermo-optical regulation and control method | |
| CN112772472A (en) | Ecological breeding pool and ecological breeding method for wild black porgy | |
| KR101476032B1 (en) | Embryo production method for mackerel | |
| JPH0249689B2 (en) | ||
| CN110419470B (en) | Indoor industrial reproduction method of mandarin fish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |