CN105145438A - Method for establishing nile tilapia merit female pure line - Google Patents
Method for establishing nile tilapia merit female pure line Download PDFInfo
- Publication number
- CN105145438A CN105145438A CN201510635512.XA CN201510635512A CN105145438A CN 105145438 A CN105145438 A CN 105145438A CN 201510635512 A CN201510635512 A CN 201510635512A CN 105145438 A CN105145438 A CN 105145438A
- Authority
- CN
- China
- Prior art keywords
- bolti
- female
- milter
- net cage
- tilapia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000276703 Oreochromis niloticus Species 0.000 title abstract description 5
- 230000004720 fertilization Effects 0.000 claims abstract description 8
- 210000000349 chromosome Anatomy 0.000 claims abstract description 6
- 241000647380 Oreochromis sp. YCC-2008 Species 0.000 claims description 15
- 102000002322 Egg Proteins Human genes 0.000 claims description 12
- 108010000912 Egg Proteins Proteins 0.000 claims description 12
- 210000004681 ovum Anatomy 0.000 claims description 12
- 230000013011 mating Effects 0.000 claims description 11
- 241000251468 Actinopterygii Species 0.000 claims description 8
- 230000002068 genetic effect Effects 0.000 claims description 6
- 230000002779 inactivation Effects 0.000 claims description 6
- 230000005070 ripening Effects 0.000 claims description 6
- 235000013601 eggs Nutrition 0.000 claims description 5
- 230000000366 juvenile effect Effects 0.000 claims description 5
- 230000009849 deactivation Effects 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 3
- 230000017448 oviposition Effects 0.000 claims description 3
- 230000008771 sex reversal Effects 0.000 claims description 3
- 230000027272 reproductive process Effects 0.000 claims 1
- 241000276707 Tilapia Species 0.000 abstract description 7
- 238000009399 inbreeding Methods 0.000 abstract 1
- 230000035800 maturation Effects 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241000219233 Oreochromis aureus Species 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 210000001766 X chromosome Anatomy 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for establishing a nile tilapia merit female pure line. Only XX female tilapia is generated through gynogenetic egg chromosome doubling, false male tilapia (XX) obtained after sex reversion can carry out fertilization normally with the female tilapia, so that fry of the later generation are all female tilapia, and in other words, the female pure line is achieved. The nile tilapia merit female pure line is established through the method of parent selecting, maturation accelerating of parents, gynogenesis, sex reversion and inbreeding, and the merits of nile tilapia as reproduction female parents are effectively maintained.
Description
Technical field
The invention belongs to the Genetic improvement technology of bolti.Especially can by Juvenile stage, accelerate the ripening, gynogenesis, gynogenesis offspring sex reverse, the male and female mating etc. after reverse, set up female bolti pure lines a kind of method.
Background technology
The Buddhist nun's Hybrid tilapia difficult to understand offspring of the mating of bolti female parent (the Oreochromis aureus male parent with) is one of main Tilapia mossambica breed variety of China, through nearly twenty or thirty year cultivation, the particularly excessive use of parent, parent's genetic diversity is increased, by traditional phenotypic character selection method, be difficult to accurately be improved in genetic character, causing the reproduction rate of female parent (bolti), the proterties such as male ratio, resistance of Buddhist nun's Hybrid tilapia seedling difficult to understand of making exists difference in various degree and differentiation, there is decline trend in kind merit.
Summary of the invention
In order to solve current Buddhist nun's Hybrid tilapia parent difficult to understand, the degradation phenomena of especially maternal Oreochromis niloticus matter merit, the invention provides a kind of a kind of method setting up the female pure lines of bolti.
Principle: bolti sex determinative chromosome is X, Y type, XY is male, XX is female, ovum only produces X chromosome ovum, the raun of XX is only produced by gynogenesis ovum chromosome doubling, false milter (XX) after property turns can with raun normal fertilization, such offspring seed is full raun, i.e. female pure lines.
The present invention for achieving the above object, adopts following technical scheme:
Set up a method for the female pure lines of bolti merit, it is characterized in that comprising the following steps:
One, Juvenile stage:
Mating season in June, in the kind that existing bolti is possessed, the individuality in 1 age is selected to breed, breeding is carried out in net cage, net cage 1 cubic metre of size, 2 tail bolti milter 3 tail bolti rauns put in a suitable place to breed by every net cage, check the situation of laying eggs (ovum is contained in raun oral cavity) of a bolti raun week about later, the bolti raun of egg laying amount more than 1500 is chosen to concentrate in special pond and cultivates as parent population for subsequent use, giving it up of less than 1500 bolti rauns.In election process, suitably supplement female parent, reach required selection quantity.
Second Year June, prepare parent population for subsequent use and 2 age red tilapia milter, separate with net cage respectively and raise, every net cage puts a tail bolti raun or red tilapia milter in a suitable place to breed, intensive culture about 15 days, when finding that the fish in some net cages no longer ingests, red tilapia milter color is beautiful when occupying one jiao, net cage, namely reaches parent and to accelerate the ripening requirement.
Two, gynogenesis:
Conveniently gynogenetic method, carry out deactivation to the sperm of red tilapia milter, the sperm of genetic inactivation and the ovum " fertilization " of bolti raun, low temperature induction ovum chromosome doubling becomes normal dliploid, then carry out artificial incubation, complete gynogenesis.
In gynogenetic fry, there is normal bolti seedling (for gynogenetic offspring), and the fry of red zone black splotch is cross hybrid seedling (not having the red tilapia milter sperm of inactivation and the female fish egg normal fertilization institute of bolti extremely), give it up.
Three, sex reverses:
Gynogenetic complete female bolti seedling, wherein 1/2nd of quantity normally cultivate, second half carries out sex reverse, obtains false milter (physiological performance is male, and genetic material is female).Cultivate for subsequent use after 1 year.
Four, endogamy:
The raun normally cultivated out after gynogenesis and the milter of sex reversal, carry out mating in mating season, and the fry of producing is the female pure lines of bolti.
Invent reversed by Juvenile stage, the accelerating the ripening of parent, gynogenesis, sex, endogamic method, set up the pure lines of bolti merit, effectively keep bolti as the maternal merit of breeding.
Embodiment
One, Juvenile stage:
Mating season in June, in the kind that existing bolti is possessed, the individuality in 1 age is selected to breed, breeding is carried out in net cage, net cage 1 cubic metre of size 60,2 tail bolti milter 3 tail bolti rauns put in a suitable place to breed by every net cage, check the situation of laying eggs (ovum is contained in raun oral cavity) of a bolti raun week about later, the bolti raun of egg laying amount more than 1500 is chosen to concentrate in special pond and cultivates as parent population for subsequent use, giving it up of less than 1500 bolti rauns.In election process, suitably supplement female parent, reach required selection quantity.Female 400 tails of bolti are chosen altogether after 1 month.
Second Year June, prepare 30 tail 2 red tilapia in age milters, good, the individual 60 larger tails of gonad development are picked out from 400 tail bolti rauns, red tilapia milter and rear parent population bolti raun for subsequent use, separate with net cage respectively and raise, every net cage puts a tail bolti raun or red tilapia milter in a suitable place to breed, intensive culture about 15 days, when finding that the fish in some net cages no longer ingests, red tilapia milter color is beautiful when occupying one jiao, net cage, namely reaches parent and to accelerate the ripening requirement.
Two, gynogenesis:
Conveniently gynogenetic method, deactivation is carried out to the sperm of red tilapia, the red tilapia sperm of genetic inactivation and the female fish egg of bolti " fertilization " (excite egg development and do not participate in genetic material transmission), low temperature induction ovum chromosome doubling becomes normal dliploid, then carry out artificial incubation, complete gynogenesis.Obtain gynogenesis of fish fry 3200 tail altogether,
Through the cultivation of 1 month, in gynogenetic fry, have normal bolti seedling (for gynogenetic offspring), and the fry of red zone black splotch is cross hybrid seedling (do not have the sperm of inactivation and ovum normal fertilization institute to), gives it up.Obtain gynogenesis of fish fry more than 2900 tail altogether.
Three, sex reverses:
In gynogenetic complete female bolti seedling 2900 tail, wherein 1/2nd of quantity normally cultivate, second half carries out sex reverse, (physiological performance is male to obtain false milter, genetic material is female), carry out sex identification after forming adult fish, obtain false milter more than 1400 tail altogether.Cultivate for subsequent use after 1 year.
Four, endogamy:
False milter 500 tail of Ni Luoluofei raun 1000 tail normally cultivated out after selecting gynogenesis and sex reversal, carries out mating in mating season, and the fry of producing is the female pure lines of bolti.Post-coitum catches seedling 900,000 tail bolti pure lines seed on the 10th day first.
Claims (5)
1. set up the method for the female pure lines of bolti merit, it is characterized in that comprising the steps:
(1) Juvenile stage:
Mating season in June, in the kind that existing bolti is possessed, select the individuality in 1 age to breed, check the situation of laying eggs of a bolti raun week about, choose the raun of egg laying amount more than 1500 as parent population for subsequent use;
Second Year June, by parent population for subsequent use and 2 age red tilapia milter, separate and raise, intensive culture 12-18 days, reach parent and to accelerate the ripening requirement;
(2) gynogenesis:
Conveniently gynogenetic method, carry out deactivation to the sperm of red tilapia milter, the ovum " fertilization " of the sperm of genetic inactivation and bolti parent population for subsequent use, low temperature induction ovum chromosome doubling becomes normal dliploid, then carry out artificial incubation, complete gynogenesis;
In gynogenetic fry, reject the cross hybrid seedling not having the ovum normal fertilization of the sperm of inactivation and bolti parent population for subsequent use;
(3) sex reverses:
Gynogenetic complete female bolti seedling, wherein 1/2nd of quantity normally cultivate, second half carries out sex reverse, obtains false milter, cultivates for subsequent use after 1 year;
(4) endogamy:
The female bolti milter of the bolti raun normally cultivated out after gynogenesis and sex reversal, carries out mating in mating season, and the fry of producing is the female pure lines of bolti.
2. the method setting up the female pure lines of bolti merit according to claim 1, is characterized in that: in described step (1), reproductive process is carried out in net cage, and net cage 1 cubic metre of size, 2 tail milter 3 tail rauns put in a suitable place to breed by every net cage.
3. the method setting up the female pure lines of bolti merit according to claim 1, is characterized in that: in described step (1) parent population for subsequent use election process in, regular replenishment bolti raun.
4. the method setting up the female pure lines of bolti merit according to claim 1, is characterized in that: separate in described step (1) to raise and adopt net cage to separate to raise, every net cage put in a suitable place to breed tail parent population for subsequent use or 2 age red tilapia milter.
5. the method setting up the female pure lines of bolti merit according to claim 4, is characterized in that: when the fish in net cage no longer ingests, and red tilapia milter color is beautiful when occupying one jiao, net cage, namely reaches parent and to accelerate the ripening requirement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510635512.XA CN105145438B (en) | 2015-09-30 | 2015-09-30 | The method for establishing bolti merit female pure lines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510635512.XA CN105145438B (en) | 2015-09-30 | 2015-09-30 | The method for establishing bolti merit female pure lines |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105145438A true CN105145438A (en) | 2015-12-16 |
| CN105145438B CN105145438B (en) | 2017-12-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510635512.XA Expired - Fee Related CN105145438B (en) | 2015-09-30 | 2015-09-30 | The method for establishing bolti merit female pure lines |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259089A (en) * | 2016-08-15 | 2017-01-04 | 安徽省农业科学院水产研究所 | A kind of method of large-scale production gynoecial loaches |
| CN108624619A (en) * | 2018-02-28 | 2018-10-09 | 西南大学 | Tilapia mossambica pgr/mstn gene pures knock out the completely sterile strain of female and construction method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411313A (en) * | 2008-11-25 | 2009-04-22 | 广州鹭业水产有限公司 | Method for producing fish seeds of genetic male red tilapia hybrids |
| CN101999332A (en) * | 2010-11-26 | 2011-04-06 | 中国水产科学研究院长江水产研究所 | Test-tube Tilapia nilotica breeding method |
| CN103583427A (en) * | 2013-11-06 | 2014-02-19 | 广西壮族自治区水产科学研究院 | Pseudo-male and full-female nile tilapia fingerling production method |
-
2015
- 2015-09-30 CN CN201510635512.XA patent/CN105145438B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411313A (en) * | 2008-11-25 | 2009-04-22 | 广州鹭业水产有限公司 | Method for producing fish seeds of genetic male red tilapia hybrids |
| CN101999332A (en) * | 2010-11-26 | 2011-04-06 | 中国水产科学研究院长江水产研究所 | Test-tube Tilapia nilotica breeding method |
| CN103583427A (en) * | 2013-11-06 | 2014-02-19 | 广西壮族自治区水产科学研究院 | Pseudo-male and full-female nile tilapia fingerling production method |
Non-Patent Citations (1)
| Title |
|---|
| 桂建芳等: "《鱼类性别和生殖的遗传基础及其人工控制》", 31 July 2007 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259089A (en) * | 2016-08-15 | 2017-01-04 | 安徽省农业科学院水产研究所 | A kind of method of large-scale production gynoecial loaches |
| CN108624619A (en) * | 2018-02-28 | 2018-10-09 | 西南大学 | Tilapia mossambica pgr/mstn gene pures knock out the completely sterile strain of female and construction method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105145438B (en) | 2017-12-01 |
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Granted publication date: 20171201 Termination date: 20180930 |