CN105165684B - The method for establishing Oreochromis aureus male pure lines - Google Patents
The method for establishing Oreochromis aureus male pure lines Download PDFInfo
- Publication number
- CN105165684B CN105165684B CN201510635367.5A CN201510635367A CN105165684B CN 105165684 B CN105165684 B CN 105165684B CN 201510635367 A CN201510635367 A CN 201510635367A CN 105165684 B CN105165684 B CN 105165684B
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- CN
- China
- Prior art keywords
- gynogenesis
- oreochromis aureus
- pure lines
- milter
- raun
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of method for establishing Oreochromis aureus male pure lines, purified milter ZZ mates with just common female Oreochromis aureus zw, and mating generates milter Zz and raun Zw and respectively accounts for 50%, fish culture to sexal maturity.Raun Zw therein, milter of the contained Z chromosome from purifying mate with the milter ZZ of gynogenesis again, and the fry of generation cultivates to sexal maturity, as a result generate a large amount of pure lines Ao Yali milters ZZ individuals.The present invention by Juvenile stage, accelerate the ripening, gynogenesis, mating selection, the method for backcrossing can establish the excellent pure lines of Oreochromis aureus, effectively keep Oreochromis aureus as the merit for breeding male parent.
Description
Technical field
This invention belongs to the Genetic improvement technology of sharp Tilapia mossambica difficult to understand.Especially can by Juvenile stage, accelerate the ripening, thelykaryon
Development, mating selection, the method for backcrossing, it is established that a kind of method of Oreochromis aureus male pure lines.
Background technology
Buddhist nun's Hybrid tilapia difficult to understand（The offspring that Oreochromis aureus male parent mates with bolti female parent）It is that China is main
One of Tilapia mossambica breed variety, cultivated by nearly twenty or thirty year, especially the excessive use of parent, make parent's genetic diversity
Increase, by traditional phenotypic character selection method, it is difficult to accurately be improved in terms of inhereditary feature, leads to the Ao Ni made
There are different degrees of difference and differentiation, kind merit exists to decline the characters such as male ratio, the resistance of Hybrid tilapia seedling
Move back trend.
Invention content
In order to solve moving back for current Buddhist nun Hybrid tilapia parent difficult to understand, especially male parent Oreochromis aureus germplasm merit
Change phenomenon, the present invention provides a kind of a kind of method for establishing Oreochromis aureus male pure lines.
Principle：The chromosome type of Oreochromis aureus Sex Determination is Z, W type, i.e. ZZ shows as male, ZW performances
For female, the excellent pure lines of Oreochromis aureus male property are obtained, it is necessary to pick out parent from existing breeding and establish.
The principle that this method establishes Oreochromis aureus male pure lines is, Oreochromis aureus raun produces two kinds of ovum, Z-type and
W types, dyed body doubles to generate the fry of two kinds of genders again after gynogenesis（And X, Y chromosome Sex Determination type thelykaryon are sent out
It educates offspring and only generates full raun XX, such as bolti）, i.e. ZZ shows as male（Pure lines）, WW shows as female（It is non-required
's）, the wilderness demand of the male fry ZZ quantity general satisfaction that generates by gynogenesis not production will carry out pure lines expansion
Greatly.Particularly purified milter ZZ mates with just common female Oreochromis aureus zw, and mating generates milter
Zz and raun Zw respectively accounts for 50%, fish culture to sexal maturity.Raun therein（Zw, milter of the contained Z chromosome from purifying）Again with
The milter of gynogenesis（ZZ）It mates, the fry of generation cultivates to sexal maturity, as a result generates a large amount of Ao Yali milters
（ZZ）Individual is sheerly.Wherein Zw types show as raun（It eliminates and does not have to）.
A method of establishing Oreochromis aureus merit female pure lines, it is characterised in that include the following steps：
One, Juvenile stage
Mating period in June selects the individual in 2 ages to be bred in the kind that existing Oreochromis aureus is possessed,
Breeding carries out in net cage, and 1 cubic metre of size of net cage, every net cage puts 2 tail Oreochromis aureus milter, 3 tail Ao Liya Rofes in a suitable place to breed
Fish raun checks the oviposition situation of a raun week about later（Ovum is contained in raun oral cavity）, egg laying amount 1500 with
On raun choose and concentrate in special pond cultivation and be used as spare parent population, 1500 or less rauns are given it up.In election process
In, female parent is suitably supplemented, required selection quantity is reached.
Second year June, the spare parent population of preparation and 2 age red tilapia milters are separated with net cage raise respectively, every net cage
Put a tail Oreochromis aureus raun or red tilapia milter in a suitable place to breed, intensive culture 15 days or so, when finding the fish in some net cages no longer
It ingests, red tilapia milter color is beautiful when occupying one jiao of net cage, that is, reaches parent and accelerate the ripening requirement.
Two, gynogenesis
According to the method for conventional gynogenesis, the sperm of red tilapia milter is inactivated, the essence of genetic inactivation
Son and the ovum " fertilization " of Oreochromis aureus raun, low temperature induction ovum chromosome doubling at normal diploid, then into
Gynogenesis is completed in row artificial incubation.
In the fry of gynogenesis, there is normal Oreochromis aureus seedling（For the offspring of gynogenesis, wherein WW（♀）With
ZZ（♂）Respectively account for 50%）, and the fry of red zone black splotch is cross hybrid seedling（The sperm not inactivated and ovum normal fertilization institute
Extremely）, give it up.The offspring fry of gynogenesis cultivates at sexal maturity, wherein there is milter（ZZ）（Account for individual in population quantity
50%）As male pure lines Oreochromis aureus caused by gynogenesis.
Three, mating selection
The male pure lines Oreochromis aureus ZZ that gynogenesis generates, mates with normal raun zw, generates milter
Zz and raun Zw fries, fry cultivate to sexal maturity, eliminate milter Zz therein, and selection retains raun Zw therein and is used for back
It hands over.
Four, it is returned
It selects the raun Zw retained to be returned with male pure lines Oreochromis aureus ZZ caused by gynogenesis, produces
To sexal maturity, the Z chromosome of milter ZZ therein is all from gynogenesis for the fry cultivation gone out, as group expand after Austria
Leah male is sheerly Tilapia mossambica.
The present invention by Juvenile stage, accelerate the ripening, gynogenesis, mating selection, the method for backcrossing can establish Leah sieve difficult to understand
The excellent pure lines of non-fish effectively keep Oreochromis aureus as the merit of breeding male parent.
Specific implementation mode
One, Juvenile stage
Mating period in June selects the individual in 2 ages to be bred in the kind that existing Oreochromis aureus is possessed,
Breeding carries out in net cage, 1 cubic metre of net cage size 60, and it is female that every net cage puts 2 tail milter, 3 tail in a suitable place to breed, examines week about later
Look into the oviposition situation of a raun（Ovum is contained in raun oral cavity）, egg laying amount is chosen in 1500 or more rauns concentrates on special pond
Middle cultivation is used as spare parent population, and 1500 or less rauns are given it up.In election process, female parent is suitably supplemented, is reached
To required selection quantity.Choose female 300 tail of Oreochromis aureus after 1 month altogether.
Second year June prepares 30 tail, 2 age red tilapia milter, gonad development is picked out from 300 tail Oreochromis aureus
60 tails good, individual is larger, red tilapia milter and rear spare parent population Oreochromis aureus raun are separated with net cage raise respectively
It supports, every net cage puts a tail raun or milter in a suitable place to breed, intensive culture 15 days or so, when finding that the fish in some net cages no longer ingests, milter
Color is beautiful when occupying one jiao of net cage, that is, reaches parent and accelerate the ripening requirement.
Two, gynogenesis
According to the method for conventional gynogenesis, the sperm of red tilapia is inactivated, the sperm of genetic inactivation with
Oreochromis aureus ovum " fertilization "（It excites egg development and is not involved in inhereditary material transmission）, low temperature induction ovum chromosome adds
Again at normal diploid, artificial incubation is then carried out, completes gynogenesis.2800 tail of gynogenesis of fish fry is obtained altogether,
By cultivation in 3 months, in the fry of gynogenesis, there is normal Oreochromis aureus seedling（For gynogenesis
Offspring）, and the fry of red zone black splotch is cross hybrid seedling（The sperm not inactivated and ovum normal fertilization institute are extremely）, abandon no
With.Removal deformity and slow-growing individual, obtain more than 200 tail of gynogenesis fishes altogether.Reservation wherein has more than 100 tail milters
（ZZ）, choose raun（WW）.
Three, mating selection
The male pure lines Oreochromis aureus that gynogenesis generates（ZZ）In 100 tails, selects gonad development and well amount to 30
Tail, with normal raun（zw）100 tails mate, and obtain 60000 tail of fry altogether（40% raun oviposition, 1500 are averagely produced per tail
Tail or so）Fry cultivates to sexal maturity, eliminates milter therein（Zz）, selection reservation 30000 tail of raun therein, after forming
To raun（Zw）More than 10000 tails are for being returned.It is selected to retain raun according to production scale.（It lays eggs by 40% raun, is produced per tail
1500 tail fries, 10000 rauns can produce 6,000,000 tail seedlings, wherein 3,000,000 tails are male Oreochromis aureus）
Four, it is returned
Select the raun retained（Zw）400 tails are selected in 10000 tails, are sheerly with male caused by gynogenesis（ZZ）
100 tails are returned, the 240000 tail fries cultivation produced to sexal maturity, 120000 tail milter therein（ZZ）Z dyeing
Body is all from gynogenesis, and the Ao Liya males after as group expands are sheerly Tilapia mossambica.
Claims (6)
1. the method for establishing Oreochromis aureus male pure lines, it is characterised in that include the following steps：
（1）Juvenile stage：
Mating period in June selects the individual in 2 ages to be bred in the kind that existing Oreochromis aureus is possessed, every
The oviposition situation of the one Oreochromis aureus raun of weekly check one time chooses egg laying amount in 1500 or more rauns as spare parent
Fish；
Spare parent population and 2 age red tilapia milters are separated raising by second year June, intensive culture 12-18 days, are reached parent and are accelerated the ripening and want
It asks；
（2）Gynogenesis：
According to the method for conventional gynogenesis, the sperm of red tilapia milter is inactivated, the sperm of genetic inactivation with
The ovum " fertilization " of the spare parent population of Oreochromis aureus, low temperature induction ovum chromosome doubling at normal diploid, then into
Gynogenesis is completed in row artificial incubation；
In the fry of gynogenesis, the ovum normal fertilization for the sperm and spare parent population of Oreochromis aureus not inactivated is rejected
Cross hybrid seedling, at sexal maturity, wherein milter is that male pure lines caused by gynogenesis are difficult to understand for the offspring fry cultivation of gynogenesis
Leah Tilapia mossambica（ZZ）；
（3）Mating selection：
The male pure lines Oreochromis aureus that gynogenesis generates（ZZ）, with normal Oreochromis aureus raun（zw）It is handed over
Match, fry cultivates to sexal maturity, eliminates milter therein（Zz）, selection reservation raun therein（Zw）For being returned；
（4）Backcrossing：
Select the raun retained（Zw）With male pure lines Oreochromis aureus caused by gynogenesis（ZZ）It is returned,
The fry produced cultivates to sexal maturity, milter therein（ZZ）Z chromosome be all from gynogenesis, as group
Ao Liya males after expansion are sheerly Tilapia mossambica.
2. the method according to claim 1 for establishing Oreochromis aureus male pure lines, it is characterised in that：The step
（1）Middle reproductive process carries out in net cage, and 1 cubic metre of size of net cage, every net cage puts 2 tail milter, 3 tail raun in a suitable place to breed.
3. the method according to claim 1 for establishing Oreochromis aureus male pure lines, it is characterised in that：The step
（1）In spare parent population election process in, regular replenishment Oreochromis aureus raun.
4. the method according to claim 1 for establishing Oreochromis aureus male pure lines, it is characterised in that：The step
（1）Middle separation raising separates raising using net cage, and every net cage puts the spare parent population of a tail or 2 age red tilapia milters in a suitable place to breed.
5. the method according to claim 4 for establishing Oreochromis aureus male pure lines, it is characterised in that：When in net cage
Fish no longer ingests, and red tilapia milter color is beautiful when occupying one jiao of net cage, that is, reaches parent and accelerate the ripening requirement.
6. the method according to claim 1 for establishing Oreochromis aureus male pure lines, it is characterised in that：The step
（2）Male pure lines Oreochromis aureus caused by middle gynogenesis（ZZ）Account for the 50% of the fry of gynogenesis.
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| CN201510635367.5A CN105165684B (en) | 2015-09-30 | 2015-09-30 | The method for establishing Oreochromis aureus male pure lines |
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| CN105994077A (en) * | 2016-06-30 | 2016-10-12 | 湖南文理学院 | Efficient breeding method for spiral shell male tetraploid pure line |
| CN106818546B (en) * | 2016-12-05 | 2020-04-17 | 广西壮族自治区水产科学研究院 | Breeding method of high-reproduction type hybrid mating mantle line of Niao tilapia |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994072A (en) * | 2006-12-28 | 2007-07-11 | 中国科学院水生生物研究所 | Method for continuously producing complete male pelteobagrus fulvidraco by sex reversion and embryonic development |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994072A (en) * | 2006-12-28 | 2007-07-11 | 中国科学院水生生物研究所 | Method for continuously producing complete male pelteobagrus fulvidraco by sex reversion and embryonic development |
Non-Patent Citations (1)
| Title |
|---|
| 奥略罗非鱼的性转换、全雄性种群的产生及其生长;叶冀雄;《外国水产》;19830331(第1期);第3-9页 * |
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