CN104705259B - In silkworm genetic breeding dark moth wing base because Application way - Google Patents
In silkworm genetic breeding dark moth wing base because Application way Download PDFInfo
- Publication number
- CN104705259B CN104705259B CN201510044662.3A CN201510044662A CN104705259B CN 104705259 B CN104705259 B CN 104705259B CN 201510044662 A CN201510044662 A CN 201510044662A CN 104705259 B CN104705259 B CN 104705259B
- Authority
- CN
- China
- Prior art keywords
- individual
- moth wing
- gene
- homozygous
- dark
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 36
- 238000012214 genetic breeding Methods 0.000 title claims abstract description 8
- 108700003861 Dominant Genes Proteins 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 18
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims abstract description 11
- 230000001488 breeding effect Effects 0.000 claims abstract description 9
- 230000008569 process Effects 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 235000013601 eggs Nutrition 0.000 claims description 7
- 230000009466 transformation Effects 0.000 claims description 6
- 230000007547 defect Effects 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 abstract description 4
- 108091006146 Channels Proteins 0.000 abstract description 3
- 238000007796 conventional method Methods 0.000 description 5
- 108700005079 Recessive Genes Proteins 0.000 description 4
- 102000052708 Recessive Genes Human genes 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 241000382353 Pupa Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses dark moth wing base in silkworm genetic breeding because Application way, homozygous method for genetic is quickly introduced by a kind of dominant gene, by in a kind of the channel genes to cross combination, make it on the basis of the degree of being practical of economic characters is not reduced, and can using its in cross combination another pair hand over kind between exist significant moth wing body colour difference reach distinguish and reject it is pure right, so as to improve the purpose of first cross kind hybrid rate.Quick homozygous method for genetic after channel genes is to utilize the inherent characteristic of the dominant gene homozygous individual and select the type individual subculture, can accelerate the complete homozygous process of the gene, can significantly reduce selection algebraically relative to traditional breeding way.Algebraically needed for gene pure of the present invention is few, and process is simple, and cost is low, pollution-free, economic characters are good, and practical value is high, and using dark moth wing base in cross combination because preparation feature, the hybrid rate of first cross kind can be greatly improved, avoid the underproof phenomenon of hybrid rate index from occurring.
Description
Technical field
The present invention relates to the breeding method of silkworm, particularly relate to dark moth wing base in silkworm genetic breeding because utilization side
Method.
Background technology
According to the regulation of national silkworm first cross kind inspection procedure standard (NYT 327-1997), first cross kind it is miscellaneous
Friendship rate must not be less than 95%, and traditional male and female pupa authentication technique is not only time-consuming, and often because technical measures are not in place straight
Connect cause because male and female are not thorough respectively and largely occur it is pure to mating, hybrid rate index be difficult to it is up to standard, cause by the gross silkworm egg scrap.
In the prior art, 2 kinds avoid the pure technical method to generation in silkworm breeding production：
1st, some silkworm egg production units are dyed a kind silkworm moth in Hybrid using dyestuff, and it is pure right to reach differentiation
Purpose, but such a method can not only increase recruitment and the input cost during kind of the field production of hybrid seeds, and due to having used industry
Dyestuff, cause silkworm moth to continue to apply to deep processing (such as male bombycic wine), produce new environmental pollution, do not meet current " money
Source save, it is environment-friendly " requirement；
2nd, other silkworm egg production units are grey black using the silkworm moth of homozygous darkening property gene (mln/mln), in silkworm
The kind that the recessive gene is imported into cross combination in breeding process, it is grey black to cause the kind silkworm moth body colour,
It is obvious with ordinary white silkworm moth difference, it is caused pure right in easily distinguishable first cross kind manufacturing process, but due to the basic product
Cocoon weight, cocoon shell weight, the Cocoon layer ratio of kind are relatively low, and economic characters are poor, and vitality is relatively low, no practical value, need to constantly transform,
Especially because gene linkage, is difficult to increase substantially its practicality by Chromosome recombination technology, constrains the veriety mesh
Preceding popularization and application.
The content of the invention
The problem of existing for prior art, solving artificially colored technology it is an object of the invention to provide one kind causes dirt
The method of dye and the darkening property not high technical barrier of genetic resources degree of being practical, will be sent out using this method in practical kind
Existing dark moth wing genetic resources, homozygous method is quickly introduced by its channel genes to cross combination by a kind of dominant gene
In a kind, make it on the basis of the degree of being practical of economic characters is not reduced, and can using its with it is another in cross combination
Hand over for a pair between kinds significant moth wing body colour difference reach distinguish and reject it is pure right, so as to improve the mesh of first cross kind hybrid rate
's.Meanwhile shade has the characteristics of larger difference between the homozygous individual and heterozygous individual based on the dominant gene, in seed selection
The completely homozygous purpose of dominant gene can be rapidly achieved by the individual subculture for selecting color most deep in journey, relative to traditional breeding method
Algebraically needed for dominant gene homozygosis in method, the method can significantly reduce the homozygous selection subculture number of the dominant gene,
And it is homozygous thorough, no recessive gene c is present.
In silkworm genetic breeding provided by the invention dark moth wing base because Application way, comprise the following steps：
(1) cultivated silkworm breed variety that need to be transformed is chosen as parent；
(2) with containing dark moth wing base because basic silkworm race with selected by need transformation cultivated silkworm breed variety hybridized to obtain heterozygosis
Type F1, utilize F1Or backcrossing each generation obtained goneoclin carries out continuous backcross with the cultivated silkworm breed variety that selection need to transform and returned
Seed selection material is handed over, recycles backcross breeding material to be selfed to obtain F2, from F2The individual selfing for selecting moth wing body colour most deep in generation obtains
F3.Because dark moth wing base is because of dominant gene, and the color of dominant gene homozygous individual is most deep, the non-homozygous individual of dominant gene
The color of (heterozygous individual) is shallower than the color of its homozygous individual, based on this otherness feature, dominant gene can be made to reach homozygous mesh
Algebraically foreshorten to F3Generation, i.e. F2The most deep dominant gene homozygous individual of generation selection moth wing color comes subculture, F3In generation, just can reach
Dominant dark moth wing base is because of the purpose of 100% homozygosis.Finally, obtain that general performance is excellent, dark moth wing by continuing seed selection
The completely homozygous new varieties of gene；
Need to import dominant dark moth wing gene C in transformation material cc to selected；
All dominant gene heterozygous individuals, need to transform material cc selected by backcrossing, and 50%；
Need to transform material cc selected by selection Cc individual backcrossings, 75%；
Need to transform material cc selected by selection Cc individual backcrossings, 87.5%；
Need to transform material cc selected by selection Cc individual backcrossings, 93.75%；
Cc individual selfings are selected, prepare F2, the theoretical proportions 1- (1/2) of contained blood relationship compositionn+1；
The CC individual selfing subcultures that color is most deep are selected, and conventional method is to eliminate cc individuals；
Wherein CC individuals are 100%, and conventional method is to continue with eliminating cc individuals in CC, Cc, cc colony, until F8Newly
Ratio containing dominant gene C in seed selection material is more than 99%, but recessive gene c is still with the presence of minor proportion (dark moth wing
Gene is represented with C, is dominant；Ordinary white moth wing is represented with c, for recessiveness；CC represents dark moth wing base because of homozygous individual, color
It is most deep；Cc represents dark moth wing base because heterozygous individual, color are shallower；Cc represents ordinary white individual；F1~BC3Percentage afterwards
Refer to subculture individual containing the selected theoretical proportions for needing to transform material blood relationship composition).
(3) the characteristic new varieties obtained with step (2), new one is combined for another kind of ordinary white with moth wing
Combined for cenospecies, the characteristics of significant difference, i.e. a kind be present using moth wing body colour between two kinds in the cross combination
For dark moth wing, another is ordinary white moth wing, can 100% distinguish and reject in first cross kind manufacturing process there may be
It is pure right, so as to reach improve f_1 hybrid eggs of silkworm hybrid rate purpose.
The present invention is in F2For when select the most deep individual subculture of moth wing color its gene pure algebraically can be foreshortened into F3Generation,
It is that a kind of special dominant gene is completely pure relative to the homozygous basic step of dominant gene in silkworm genetic breeding conventional method
The fast method of conjunction.
The present invention have the advantage that compared with prior art for：Gene pure algebraically of the present invention is few, and process is simple, into
This is low, and without any pollution, economic characters are good, and practical value is high, and using hybridize dark moth wing base in Combination nova because preparation it is special
Point, i.e. kind are dark flower wing, and another is ordinary white, can greatly improve the hybrid rate of first cross kind, avoid because
Hybrid rate index is unqualified and phenomenon that silkworm egg is eliminated by the gross occurs.
Brief description of the drawings
Fig. 1 is the hybridization flow chart of the present invention.
Embodiment
Be as shown in Figure 1 the invention provides dark moth wing base in silkworm genetic breeding because Application way, it is including as follows
Step：
(1) cultivated silkworm breed variety that need to be transformed is chosen as parent；
(2) with containing dark moth wing base because basic silkworm race with selected by need transformation cultivated silkworm breed variety hybridized to obtain heterozygosis
Type F1, utilize F1Or the goneoclin that backcrossing each generation obtains is returned with the selected cultivated silkworm breed variety progress continuous backcross for needing transformation
Seed selection material is handed over, recycles backcross breeding material to be selfed to obtain F2, from F2The individual selfing for selecting moth wing body colour most deep in generation obtains
F3.Because dark moth wing base is because of dominant gene, and the color of dominant gene homozygous individual is most deep, the non-homozygous individual of dominant gene
The color of (heterozygous individual) is shallower than the color of its homozygous individual, based on this otherness feature, dominant gene can be made to reach homozygous mesh
Algebraically foreshorten to F3Generation, i.e. F2The most deep dominant gene homozygous individual of generation selection moth wing color comes subculture, F3In generation, just can reach
Dominant dark moth wing base is because of the purpose (as shown in Figure 1) of 100% homozygosis.Finally, by continuing seed selection, to obtain general performance excellent
Good, dark moth wing base is because of complete homozygous new varieties；
Need to import dominant dark moth wing gene C in transformation material cc to selected；
All dominant gene heterozygous individuals, need to transform material cc selected by backcrossing, and 50%；
Need to transform material cc selected by selection Cc individual backcrossings, 75%；
Need to transform material cc selected by selection Cc individual backcrossings, 87.5%；
Need to transform material cc selected by selection Cc individual backcrossings, 93.75%；
Cc individual selfings are selected, prepare F2, the theoretical proportions 1- (1/2) of contained blood relationship compositionn+1；
The CC individual selfing subcultures that color is most deep are selected, and conventional method is to eliminate cc individuals；
Wherein CC individuals are 100%, and conventional method is to continue with eliminating cc individuals in CC, Cc, cc colony, until F8Newly
Ratio containing dominant gene C in seed selection material is more than 99%, but recessive gene c is still with the presence of minor proportion；
Wherein, dark moth wing base is dominant because being represented with C；Ordinary white moth wing is represented with c, for recessiveness；CC represents dark
Moth wing base is most deep because of homozygous individual, color；Cc represents dark moth wing base because heterozygous individual, color are shallower；Cc represents ordinary white
Individual；F1~BC3Percentage afterwards refers to subculture individual containing the selected theoretical proportions for needing to transform material blood relationship composition；
(3) the characteristic new varieties obtained with step (2), new one is combined for another kind of ordinary white with moth wing
Combined for cenospecies, the characteristics of significant difference, i.e. a kind be present using moth wing body colour between two kinds in the cross combination
For dark moth wing, another is ordinary white moth wing, can 100% distinguish and reject in first cross kind manufacturing process there may be
It is pure right, so as to reach improve f_1 hybrid eggs of silkworm hybrid rate purpose.
Dark moth wing base is because of dominant gene, and the moth wing color of dominant gene homozygous individual is most deep, and dominant gene is not pure
The color for closing individual (i.e. dominant gene heterozygous individual) is shallower than its homozygous individual.It is special based on this otherness during step (2)
Point, it can will come to eliminate recessive individual by generation at least through 8 algebraically more than generation in traditional breeding way, reach dominant gene homozygosis
Required algebraically foreshorten to F3Generation (F1For all dominant gene heterozygous individuals, F2Occurs partial dominance gene pure in colony
Individual).That is F2The most deep dominant gene homozygous individual subculture of generation selection moth wing color, F3In generation, just can reach dominant dark moth wing
The completely homozygous purpose of gene.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (1)
1. in silkworm genetic breeding dark moth wing base because Application way, it is characterised in that comprise the following steps：
(1) cultivated silkworm breed variety that need to be transformed is chosen as parent；
(2) with containing dark moth wing base because basic silkworm race with selected by need transformation cultivated silkworm breed variety hybridized to obtain heterozygous F1,
Utilize F1Or backcrossing each generation obtained goneoclin and the cultivated silkworm breed variety that selection need to transform carry out continuous backcross and obtain backcrossing choosing
Material is educated, recycles backcross breeding material to be selfed to obtain F2, from F2The individual selfing for selecting moth wing body colour most deep in generation obtains F3；
Because dark moth wing base is because of dominant gene, and the color of dominant gene homozygous individual is most deep, the face of dominant gene heterozygous individual
Color is shallower than the color of its homozygous individual, and based on this otherness feature, 8 seed selections more than generation will can be at least needed in traditional breeding way
Algebraically eliminates recessive individual by generation, and the algebraically for reaching dominant gene homozygosis purpose foreshortens to F3Generation, in this Breeding Process, F1
For all dominant gene heterozygous individuals, F2It is individual and dominant in this section for partial dominance gene pure occurs in colony
Defect individual is selected to come subculture, F in gene pure individual3In generation, just can reach dominant dark moth wing base because of the mesh of 100% homozygosis
's；Finally, obtain that general performance is excellent, dark moth wing base is because of complete homozygous new varieties by continuing seed selection；
(3) the characteristic new varieties obtained with step (2), for another kind of ordinary white, to combine a new generation miscellaneous with moth wing
Kind of a combination is handed over, the characteristics of significant difference be present using moth wing body colour between two kinds in the cross combination, i.e. a kind is deep
Color moth wing, another is ordinary white moth wing, can 100% distinguish and reject issuable pure in first cross kind manufacturing process
It is right, so as to reach the purpose for improving f_1 hybrid eggs of silkworm hybrid rate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044662.3A CN104705259B (en) | 2015-01-25 | 2015-01-25 | In silkworm genetic breeding dark moth wing base because Application way |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044662.3A CN104705259B (en) | 2015-01-25 | 2015-01-25 | In silkworm genetic breeding dark moth wing base because Application way |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104705259A CN104705259A (en) | 2015-06-17 |
| CN104705259B true CN104705259B (en) | 2018-03-27 |
Family
ID=53405363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510044662.3A Active CN104705259B (en) | 2015-01-25 | 2015-01-25 | In silkworm genetic breeding dark moth wing base because Application way |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104705259B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU793540A1 (en) * | 1979-07-24 | 1981-01-07 | Главное Управление Шелководствауправления Сельского Хозяйстваузбекской Ccp | Device for obtaining silkworm seed |
| CN1194093A (en) * | 1997-03-21 | 1998-09-30 | 胡祥平 | Technology for artificially cultivating china wood frog |
| CN1296741A (en) * | 2000-11-09 | 2001-05-30 | 上海丰康科技开发有限公司 | Technique for reproducing and raising Chinese wood frog in souch of China |
| RO125576A0 (en) * | 2009-11-25 | 2010-07-30 | Universitatea De Ştiinţe Agricole Şi Medicina Veterinară Cluj-Napoca | Methods for maintaining the breeds in the genetic fund of bombyx mori l species |
| CN201640211U (en) * | 2010-04-20 | 2010-11-24 | 张凤春 | Tadpole hatching breeding pool |
| CN102972360A (en) * | 2012-12-28 | 2013-03-20 | 湖南省蚕桑科学研究所 | Method for applying silkworm moth dark flowery wing gene to domestic silkworm genetic breeding |
-
2015
- 2015-01-25 CN CN201510044662.3A patent/CN104705259B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU793540A1 (en) * | 1979-07-24 | 1981-01-07 | Главное Управление Шелководствауправления Сельского Хозяйстваузбекской Ccp | Device for obtaining silkworm seed |
| CN1194093A (en) * | 1997-03-21 | 1998-09-30 | 胡祥平 | Technology for artificially cultivating china wood frog |
| CN1296741A (en) * | 2000-11-09 | 2001-05-30 | 上海丰康科技开发有限公司 | Technique for reproducing and raising Chinese wood frog in souch of China |
| RO125576A0 (en) * | 2009-11-25 | 2010-07-30 | Universitatea De Ştiinţe Agricole Şi Medicina Veterinară Cluj-Napoca | Methods for maintaining the breeds in the genetic fund of bombyx mori l species |
| CN201640211U (en) * | 2010-04-20 | 2010-11-24 | 张凤春 | Tadpole hatching breeding pool |
| CN102972360A (en) * | 2012-12-28 | 2013-03-20 | 湖南省蚕桑科学研究所 | Method for applying silkworm moth dark flowery wing gene to domestic silkworm genetic breeding |
Non-Patent Citations (2)
| Title |
|---|
| 家蚕育种的理论与方法;长岛荣一;《蚕学通讯》;19861231(第04期);第1-8页 * |
| 桑蚕暗花蛾突变体及其在生产上的应用前景;杨明观等;《蚕桑通报》;20011228;第32卷(第4期);第19-21页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104705259A (en) | 2015-06-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lou et al. | Advances in research on Cordyceps militaris degeneration | |
| CN102550485A (en) | Method for breeding improved mutton sheep strain | |
| CN105494239A (en) | Auto-sexing breeding method of early-maturing excellent type yellow-shank partridge chickens | |
| CN103416333A (en) | Litopenaeus vannamei base population establishing method based on genetic information and excellent properties | |
| CN107667976A (en) | A kind of producing method for seed of the high yield type reed catkins feather layer of green-shell egg of automatic sexing | |
| CN104642262A (en) | Breeding method for local chicken variety resource protection and utilization | |
| CN103583470A (en) | Molecular-assisted-selection cultivation method for green-shin recessive-white-feather chicken line | |
| CN102100211A (en) | Method for breeding white Rex rabbit new variety | |
| CN104604794A (en) | Recessive white feather Beijing fatty chicken breeding method | |
| CN103409420B (en) | Y-chromosome specific molecular marker of Nile tilapia, and genetic sex determination method and supermale producing method both based on molecular marker | |
| CN101926306A (en) | Breeding method of colourful rabbits | |
| CN107410191A (en) | A kind of breeding method of reed catkins feather layer of green-shell egg | |
| CN101107925B (en) | Multicomponent cross breeding method of selection and breeding of silkworm | |
| CN100420374C (en) | Hybrid breeding method for high-yield reversible-resisting ctenoid pectinid and luxurious ctenoid pectinid | |
| CN106929594A (en) | The molecular labeling of the complete red bud mutation character site of the operatic circle skin and its primer and application | |
| CN104705259B (en) | In silkworm genetic breeding dark moth wing base because Application way | |
| CN103190381B (en) | Double back hands over the breeding method of selection and breeding of silkworm multiple cross kind | |
| CN104642264A (en) | Fast balance breeding method for native hen varieties | |
| CN104642265B (en) | A kind of Fast-Balance breeding method of high-quality chicken breed | |
| Mogili et al. | Evaluation of promising interspecific hybrid derivatives of okra (Abelmoschus esculentus (L.) Moench) | |
| CN115251004B (en) | Cultivation method of green-shank yellow-feather high-quality broilers | |
| CN102422825A (en) | Method for establishing and breeding three matched breeding systems of Eriocheir sinensis | |
| Ramasundaram et al. | Manipulating technology for surplus extraction: The case of Bt cotton in India | |
| CN105385768A (en) | Method for identifying foxtail millet variety by adopting SSR molecular marker technique and application | |
| CN113207681B (en) | Breeding method of recessive glandless cotton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |