CN104704113B - Car酶及脂肪醇的改良生产 - Google Patents

Car酶及脂肪醇的改良生产 Download PDF

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Publication number
CN104704113B
CN104704113B CN201380026546.8A CN201380026546A CN104704113B CN 104704113 B CN104704113 B CN 104704113B CN 201380026546 A CN201380026546 A CN 201380026546A CN 104704113 B CN104704113 B CN 104704113B
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mutations
host cell
fatty
recombinant host
fatty alcohol
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CN104704113A (zh
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德里克·L·格林菲尔德
伊利莎白·J·克拉克
伊莱·S·格罗班
维克朗斯·阿拉盖达
S·李
X·李
Z·胡
B·朱
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Genomatica Inc
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LS9 Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006—Carboxylate reductase (1.2.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380026546.8A 2012-04-02 2013-04-02 Car酶及脂肪醇的改良生产 Active CN104704113B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Publications (2)

Publication Number Publication Date
CN104704113A CN104704113A (zh) 2015-06-10
CN104704113B true CN104704113B (zh) 2018-01-23

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US (4) US9340801B2 (enExample)
EP (6) EP3674399B1 (enExample)
JP (1) JP6230594B2 (enExample)
KR (6) KR101831331B1 (enExample)
CN (1) CN104704113B (enExample)
AU (2) AU2013243602B2 (enExample)
BR (2) BR112014024674B1 (enExample)
CA (1) CA2883064A1 (enExample)
CO (1) CO7170148A2 (enExample)
ES (3) ES2786633T3 (enExample)
MX (2) MX343296B (enExample)
MY (1) MY170889A (enExample)
SI (1) SI3385374T1 (enExample)
WO (1) WO2013152052A2 (enExample)

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US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN106574238B (zh) * 2014-07-18 2021-09-21 基因组股份公司 微生物的脂肪二醇产生
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
EP3430133A1 (en) * 2016-03-17 2019-01-23 INVISTA Textiles (U.K.) Limited Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
WO2020047088A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
WO2022209763A1 (ja) 2021-03-30 2022-10-06 旭化成株式会社 カルボン酸還元活性を有する組換えポリペプチド
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

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MX2014011903A (es) 2015-05-12
KR20190022935A (ko) 2019-03-06
EP3385374A1 (en) 2018-10-10
CA2883064A1 (en) 2013-10-10
SI3385374T1 (sl) 2020-09-30
KR102114841B1 (ko) 2020-05-26
JP6230594B2 (ja) 2017-11-15
KR102185762B1 (ko) 2020-12-02
BR112014024674A2 (pt) 2018-11-21
EP3985108A1 (en) 2022-04-20
KR20150032660A (ko) 2015-03-27
EP3153579A1 (en) 2017-04-12
KR101831331B1 (ko) 2018-02-23
US9758769B2 (en) 2017-09-12
MY170889A (en) 2019-09-12
BR112014024674B1 (pt) 2022-06-07
KR102282677B1 (ko) 2021-07-28
EP2834350A2 (en) 2015-02-11
ES2786633T3 (es) 2020-10-13
KR20200038559A (ko) 2020-04-13
AU2016231497A1 (en) 2016-10-06
EP3153579B1 (en) 2018-03-14
US20200109374A1 (en) 2020-04-09
EP3385374B1 (en) 2020-01-08
WO2013152052A3 (en) 2014-03-13
AU2016231497B2 (en) 2017-08-31
BR122019026987B1 (pt) 2022-01-18
KR20190076059A (ko) 2019-07-01
US20180135028A1 (en) 2018-05-17
JP2015512645A (ja) 2015-04-30
EP4186974A1 (en) 2023-05-31
EP2834350B1 (en) 2016-11-16
KR20170113710A (ko) 2017-10-12
MX343296B (es) 2016-11-01
US10301603B2 (en) 2019-05-28
ES2613553T3 (es) 2017-05-24
AU2013243602A1 (en) 2014-11-13
US9340801B2 (en) 2016-05-17
EP3674399A1 (en) 2020-07-01
WO2013152052A2 (en) 2013-10-10
KR20210095226A (ko) 2021-07-30
US20150064757A1 (en) 2015-03-05
AU2013243602B2 (en) 2016-11-24
MX358177B (es) 2018-08-08
US11034937B2 (en) 2021-06-15
US20160326499A1 (en) 2016-11-10
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
CN104704113A (zh) 2015-06-10
CO7170148A2 (es) 2015-01-28

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