CN104704113B - Car酶及脂肪醇的改良生产 - Google Patents

Car酶及脂肪醇的改良生产 Download PDF

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Publication number
CN104704113B
CN104704113B CN201380026546.8A CN201380026546A CN104704113B CN 104704113 B CN104704113 B CN 104704113B CN 201380026546 A CN201380026546 A CN 201380026546A CN 104704113 B CN104704113 B CN 104704113B
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China
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mutations
host cell
fatty
recombinant host
fatty alcohol
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Expired - Fee Related
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Chinese (zh)
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CN104704113A (zh
Inventor
德里克·L·格林菲尔德
伊利莎白·J·克拉克
伊莱·S·格罗班
维克朗斯·阿拉盖达
S·李
X·李
Z·胡
B·朱
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Genomatica Inc
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REG Life Sciences LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0105—Long-chain-fatty-acyl-CoA reductase (1.2.1.50)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/99—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with other acceptors (1.2.99)
    • C12Y102/99006—Carboxylate reductase (1.2.99.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01041—Beta-ketoacyl-acyl-carrier-protein synthase I (2.3.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380026546.8A 2012-04-02 2013-04-02 Car酶及脂肪醇的改良生产 Expired - Fee Related CN104704113B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261619309P 2012-04-02 2012-04-02
US61/619,309 2012-04-02
PCT/US2013/035040 WO2013152052A2 (en) 2012-04-02 2013-04-02 Novel car enzymes and improved production of fatty alcohols

Publications (2)

Publication Number Publication Date
CN104704113A CN104704113A (zh) 2015-06-10
CN104704113B true CN104704113B (zh) 2018-01-23

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CN201380026546.8A Expired - Fee Related CN104704113B (zh) 2012-04-02 2013-04-02 Car酶及脂肪醇的改良生产

Country Status (14)

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US (4) US9340801B2 (enExample)
EP (6) EP3385374B1 (enExample)
JP (1) JP6230594B2 (enExample)
KR (6) KR20170113710A (enExample)
CN (1) CN104704113B (enExample)
AU (2) AU2013243602B2 (enExample)
BR (2) BR112014024674B1 (enExample)
CA (1) CA2883064A1 (enExample)
CO (1) CO7170148A2 (enExample)
ES (3) ES2786633T3 (enExample)
MX (2) MX358177B (enExample)
MY (1) MY170889A (enExample)
SI (1) SI3385374T1 (enExample)
WO (1) WO2013152052A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010062480A2 (en) * 2008-10-28 2010-06-03 Ls9, Inc. Methods and compositions for producing fatty alcohols
MX379251B (es) * 2012-04-02 2025-03-11 Genomatica Inc Producción mejorada de derivados de ácidos grasos.
KR20170113710A (ko) * 2012-04-02 2017-10-12 알이지 라이프 사이언시스, 엘엘씨 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산
EP2882843A4 (en) 2012-08-10 2016-04-06 Opx Biotechnologies Inc MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
WO2014145343A1 (en) * 2013-03-15 2014-09-18 Opx Biotechnologies, Inc. Bioproduction of chemicals
CN106795483A (zh) 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN111748511A (zh) * 2014-07-18 2020-10-09 基因组股份公司 微生物的脂肪二醇产生
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105985987B (zh) * 2015-03-02 2020-01-31 苏州安捷生物科技有限公司 一种生物法制备脂肪醇的方法
EP3430133A1 (en) * 2016-03-17 2019-01-23 INVISTA Textiles (U.K.) Limited Carboxylic acid reductase polypeptides and variants having improved activity, materials and processes relating thereto
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
BR112019020784A2 (pt) 2017-04-03 2020-04-28 Genomatica, Inc. variantes de tioesterase com atividade melhorada para a produção de derivados de ácidos graxos de cadeia média
WO2020047304A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose
EP3844269A4 (en) 2018-08-31 2022-07-27 Genomatica, Inc. PRODUCTION OF NON-NATIVE MONOUNSATURATED FATTY ACIDS IN BACTERIA
US12649937B2 (en) 2021-03-29 2026-06-09 Genomatica, Inc. Production of non-native monounsaturated fatty acids in bacteria
US20250019671A1 (en) 2021-03-30 2025-01-16 Asahi Kasei Kabushiki Kaisha Recombinant polypeptide having carboxylic acid reducing activity
WO2023178193A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Acyl-acp thioesterase variants and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010135624A2 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols
CN102264910A (zh) * 2008-10-28 2011-11-30 Ls9公司 用于产生脂肪醇的方法和组合物
CN102325864A (zh) * 2008-12-23 2012-01-18 Ls9公司 硫酯酶相关的方法和组合物

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US7056714B2 (en) * 2003-03-11 2006-06-06 University Of Iowa Research Foundation, Inc. Carboxylic acid reductase polypeptide, nucleotide sequence encoding same and methods of use
DE102004052115A1 (de) 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
AU2007254151A1 (en) 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
CA3035878C (en) 2007-03-28 2023-01-03 REG Life Sciences, LLC Enhanced production of fatty acid derivatives
JP5912251B2 (ja) 2007-05-22 2016-06-01 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 炭化水素産生遺伝子およびその使用方法
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
EP2424989A2 (en) 2009-05-01 2012-03-07 The Regents of The University of California Product of fatty acid esters from biomass polymers
WO2011047101A1 (en) * 2009-10-13 2011-04-21 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto
WO2011100667A1 (en) 2010-02-14 2011-08-18 Ls9, Inc. Surfactant and cleaning compositions comprising microbially produced branched fatty alcohols
WO2011127409A2 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
WO2012135789A2 (en) * 2011-04-01 2012-10-04 Genomatica, Inc. Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto
EP2906587B9 (en) * 2011-04-01 2021-01-06 Genomatica, Inc. Methods and compositions for improved production of fatty acids and derivatives thereof
US8617862B2 (en) * 2011-06-22 2013-12-31 Genomatica, Inc. Microorganisms for producing propylene and methods related thereto
KR20170113710A (ko) * 2012-04-02 2017-10-12 알이지 라이프 사이언시스, 엘엘씨 Ｃａｒ 효소들 및 지방족 알코올들의 개선된 생산

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102264910A (zh) * 2008-10-28 2011-11-30 Ls9公司 用于产生脂肪醇的方法和组合物
CN102325864A (zh) * 2008-12-23 2012-01-18 Ls9公司 硫酯酶相关的方法和组合物
WO2010135624A2 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
THE REGULATION OF FATTY ACID BIOSYNTHESIS;D. Grahame HARDIE et al.;《FEBS LETTERS》;19780731;第91卷(第1期);第1-7页 *
天然脂肪醇的新型生产技术;殷福珊;《日用化学品科学》;20061231;第29卷(第12期);第4-5页 *

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Publication number Publication date
CN104704113A (zh) 2015-06-10
WO2013152052A2 (en) 2013-10-10
KR102282677B1 (ko) 2021-07-28
KR20200038559A (ko) 2020-04-13
KR102114841B1 (ko) 2020-05-26
CO7170148A2 (es) 2015-01-28
AU2013243602A1 (en) 2014-11-13
EP3385374A1 (en) 2018-10-10
EP3985108A1 (en) 2022-04-20
CA2883064A1 (en) 2013-10-10
EP2834350B1 (en) 2016-11-16
MX358177B (es) 2018-08-08
US9340801B2 (en) 2016-05-17
KR20190076059A (ko) 2019-07-01
US11034937B2 (en) 2021-06-15
ES2786633T3 (es) 2020-10-13
EP3153579B1 (en) 2018-03-14
EP3153579A1 (en) 2017-04-12
AU2016231497A1 (en) 2016-10-06
MX2014011903A (es) 2015-05-12
JP6230594B2 (ja) 2017-11-15
SI3385374T1 (sl) 2020-09-30
KR102185762B1 (ko) 2020-12-02
KR20150032660A (ko) 2015-03-27
EP3674399B1 (en) 2021-09-22
ES2666157T3 (es) 2018-05-03
AU2013243602B2 (en) 2016-11-24
JP2015512645A (ja) 2015-04-30
BR122019026987B1 (pt) 2022-01-18
WO2013152052A3 (en) 2014-03-13
KR20210095226A (ko) 2021-07-30
US9758769B2 (en) 2017-09-12
US20160326499A1 (en) 2016-11-10
KR20170113710A (ko) 2017-10-12
US20180135028A1 (en) 2018-05-17
US10301603B2 (en) 2019-05-28
EP3674399A1 (en) 2020-07-01
BR112014024674A2 (pt) 2018-11-21
ES2613553T3 (es) 2017-05-24
MX343296B (es) 2016-11-01
BR112014024674B1 (pt) 2022-06-07
KR20190022935A (ko) 2019-03-06
MY170889A (en) 2019-09-12
EP2834350A2 (en) 2015-02-11
AU2016231497B2 (en) 2017-08-31
US20150064757A1 (en) 2015-03-05
EP4186974A1 (en) 2023-05-31
KR101831331B1 (ko) 2018-02-23
EP3385374B1 (en) 2020-01-08
US20200109374A1 (en) 2020-04-09

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