CN104619836A - 具有葡糖淀粉酶活性的变体 - Google Patents

具有葡糖淀粉酶活性的变体 Download PDF

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Publication number
CN104619836A
CN104619836A CN201380042995.1A CN201380042995A CN104619836A CN 104619836 A CN104619836 A CN 104619836A CN 201380042995 A CN201380042995 A CN 201380042995A CN 104619836 A CN104619836 A CN 104619836A
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CN
China
Prior art keywords
glucoamylase
seq
amino acid
variant
parent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380042995.1A
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English (en)
Chinese (zh)
Inventor
J.F.克拉梅
P.E.德格恩
I.尼科拉伊
V.阿勒克塞耶夫
C.W.维罗伊曼
D.托雷斯帕兹米诺
N.M.菲什
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International N&H Denmark ApS
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Danisco AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco AS filed Critical Danisco AS
Publication of CN104619836A publication Critical patent/CN104619836A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00—Fermentation processes for beer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00—Preparation of wort
    • C12C7/04—Preparation or treatment of the mash
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E10/00—Energy generation through renewable energy sources
    • Y02E10/30—Energy from the sea, e.g. using wave energy or salinity gradient

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380042995.1A 2012-08-22 2013-08-21 具有葡糖淀粉酶活性的变体 Pending CN104619836A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261692040P 2012-08-22 2012-08-22
US61/692040 2012-08-22
EP12189588.2 2012-10-23
EP12189588 2012-10-23
PCT/EP2013/067391 WO2014029808A1 (en) 2012-08-22 2013-08-21 Variants having glucoamylase activity

Publications (1)

Publication Number Publication Date
CN104619836A true CN104619836A (zh) 2015-05-13

Family

ID=47046486

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380042995.1A Pending CN104619836A (zh) 2012-08-22 2013-08-21 具有葡糖淀粉酶活性的变体

Country Status (9)

Country Link
US (2) US20150315558A1 (https=)
EP (1) EP2888357A1 (https=)
JP (1) JP2015526090A (https=)
CN (1) CN104619836A (https=)
AU (1) AU2013305007A1 (https=)
EA (1) EA201590425A1 (https=)
IN (1) IN2015DN00231A (https=)
MX (1) MX2015002099A (https=)
WO (1) WO2014029808A1 (https=)

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WO2016100837A1 (en) 2014-12-18 2016-06-23 Danisco Us Inc Engineered multifunctional enzymes and methods of use
EP3253779A1 (en) 2015-02-06 2017-12-13 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CN107429220A (zh) 2015-03-27 2017-12-01 嘉吉公司 经葡糖淀粉酶修饰的酵母菌株和用于产生生物产物的方法
BR112018011902A2 (pt) 2015-12-17 2018-12-04 Cargill Inc método de fermentação, levedura geneticamente modificada, construto de ácido nucleico, vetor, célula hospedeira, meio de fermentação e utilização da levedura geneticamente modificada
JP6849316B2 (ja) * 2016-04-26 2021-03-24 サッポロビール株式会社 ビールテイスト飲料の製造方法及びビールテイスト飲料
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
JP7115830B2 (ja) * 2017-09-29 2022-08-09 群栄化学工業株式会社 麦汁もしくは麦芽エキス、又は醸造酒の製造方法
US11634673B2 (en) 2017-09-29 2023-04-25 Dupont Nutrition Biosciences Aps Production of brewer's wort having increase fermentable sugars for fermentation
BR112020018294A2 (pt) 2018-03-09 2020-12-29 Danisco Us Inc. Glucoamilases e métodos de uso dos mesmos
US20210024909A1 (en) * 2018-03-26 2021-01-28 Lallemand Hungary Liquidity Management Llc Chimeric amylases comprising an heterologous starch binding domain
CN109266633A (zh) * 2018-09-14 2019-01-25 华东理工大学 碳水化合物结合模块及其在固定化酶制备与融合蛋白纯化上的应用
WO2021236715A1 (en) * 2020-05-21 2021-11-25 Dupont Nutrition Biosciences Aps Unihibited amylases for brewing with high tannin materials
JP7096290B2 (ja) * 2020-06-12 2022-07-05 サッポロビール株式会社 ビールテイスト飲料
CN112662648B (zh) * 2021-01-04 2022-09-06 杭州荔枝红生物科技有限公司 一种木聚糖酶GH11-xylanase突变体及其应用
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法

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Also Published As

Publication number Publication date
EP2888357A1 (en) 2015-07-01
EA201590425A1 (ru) 2015-06-30
WO2014029808A1 (en) 2014-02-27
JP2015526090A (ja) 2015-09-10
MX2015002099A (es) 2015-05-11
AU2013305007A1 (en) 2015-01-29
US20150315558A1 (en) 2015-11-05
IN2015DN00231A (https=) 2015-06-12
US20170306309A1 (en) 2017-10-26

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