CN104593524A - 一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 - Google Patents
一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 Download PDFInfo
- Publication number
- CN104593524A CN104593524A CN201410831122.5A CN201410831122A CN104593524A CN 104593524 A CN104593524 A CN 104593524A CN 201410831122 A CN201410831122 A CN 201410831122A CN 104593524 A CN104593524 A CN 104593524A
- Authority
- CN
- China
- Prior art keywords
- type
- respiratory syncytial
- syncytial virus
- seq
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
名称 | 序列(5′→3′) |
A型-FP | GGTTTAAGAGAAGAAATGATAGAAAA(SEQ ID No.1) |
A型-RP1 | ATCATTGTCACTATCATTCCCTTC(SEQ ID No.2) |
A型-RP2 | ATCATTGTCACTATCGTTGTCTTC(SEQ ID No.3) |
A型-P | X1-TATGGCAAGACTCAGGAATGAGGAAAG-Y1(SEQ ID No.4) |
B型-FP | GGTTTAAGAGAAGAAATGATAGAAAA(SEQ ID No.1) |
B型-RP1 | ATCATTGTCACTATCATTCCCTTC(SEQ ID No.2) |
B型-RP2 | ATCATTGTCACTATCGTTGTCTTC(SEQ ID No.3) |
B型-P | X2-CTATCATTGGTCATTAATGCTTCCGCTCT–Y2(SEQ ID No.5) |
反应液组分 | 加量(ul)/1人份 |
核酸扩增反应液 | 7.5 |
酶混合液 | 5 |
呼吸道合胞病毒A型/B型反应液 | 4 |
去RNA酶水(阴性参考品) | 3.5 |
RNA样本 | 5 |
总体积 | 25 |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410831122.5A CN104593524B (zh) | 2014-12-26 | 2014-12-26 | 一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410831122.5A CN104593524B (zh) | 2014-12-26 | 2014-12-26 | 一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104593524A true CN104593524A (zh) | 2015-05-06 |
CN104593524B CN104593524B (zh) | 2017-06-16 |
Family
ID=53119575
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410831122.5A Active CN104593524B (zh) | 2014-12-26 | 2014-12-26 | 一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104593524B (zh) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287346A (zh) * | 2016-03-30 | 2017-10-24 | 广东省疾病预防控制中心 | 用于禽流感病毒n亚型基因分型的多重荧光定量pcr检测试剂盒及其应用 |
CN108165667A (zh) * | 2017-12-05 | 2018-06-15 | 浙江大学 | 人呼吸道合胞病毒三重实时荧光定量rt-pcr检测试剂盒 |
CN109762942A (zh) * | 2019-03-13 | 2019-05-17 | 中国疾病预防控制中心病毒病预防控制所 | 一种快速检测呼吸道合胞病毒的含内参双重等温核酸扩增方法 |
CN111778357A (zh) * | 2020-07-13 | 2020-10-16 | 国家卫生健康委科学技术研究所 | 基于CRISPR/Cas12a的呼吸道合胞病毒核酸快速检测试剂盒及其检测方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070065813A1 (en) * | 2005-09-20 | 2007-03-22 | Hayden Randall T | Assay for quantitative detection of respiratory syncytial virus (RSV) |
WO2009085733A1 (en) * | 2007-12-21 | 2009-07-09 | Quest Diagnostics Investments Incorporated | Multiplex detection assay for influenza and rsv viruses |
WO2013116965A1 (en) * | 2012-02-10 | 2013-08-15 | Beijing Advaccine Biotechnology Co. Ltd | Combined antigen and dna vaccine for preventing and treating rsv infection |
WO2013128405A1 (en) * | 2012-02-29 | 2013-09-06 | Vela Operations Pte.Ltd. | Real time pcr detection of respiratory syncytial virus |
CN103451317A (zh) * | 2011-12-15 | 2013-12-18 | 上海市公共卫生临床中心 | 一种同时检测五种呼吸道病毒的引物、探针及其用途 |
-
2014
- 2014-12-26 CN CN201410831122.5A patent/CN104593524B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070065813A1 (en) * | 2005-09-20 | 2007-03-22 | Hayden Randall T | Assay for quantitative detection of respiratory syncytial virus (RSV) |
WO2009085733A1 (en) * | 2007-12-21 | 2009-07-09 | Quest Diagnostics Investments Incorporated | Multiplex detection assay for influenza and rsv viruses |
CN103451317A (zh) * | 2011-12-15 | 2013-12-18 | 上海市公共卫生临床中心 | 一种同时检测五种呼吸道病毒的引物、探针及其用途 |
WO2013116965A1 (en) * | 2012-02-10 | 2013-08-15 | Beijing Advaccine Biotechnology Co. Ltd | Combined antigen and dna vaccine for preventing and treating rsv infection |
WO2013128405A1 (en) * | 2012-02-29 | 2013-09-06 | Vela Operations Pte.Ltd. | Real time pcr detection of respiratory syncytial virus |
Non-Patent Citations (2)
Title |
---|
AIZHONG HU,ET AL: "Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real-Time PCR", 《JOURNAL OF CLINICAL MICROBIOLOGY 》 * |
JANE KUYPERS,ET AL: "Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children", 《JOURNAL OF CLINICAL VIROLOGY》 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107287346A (zh) * | 2016-03-30 | 2017-10-24 | 广东省疾病预防控制中心 | 用于禽流感病毒n亚型基因分型的多重荧光定量pcr检测试剂盒及其应用 |
CN107287346B (zh) * | 2016-03-30 | 2020-11-06 | 广东省疾病预防控制中心 | 用于禽流感病毒n亚型基因分型的多重荧光定量pcr检测试剂盒及其应用 |
CN108165667A (zh) * | 2017-12-05 | 2018-06-15 | 浙江大学 | 人呼吸道合胞病毒三重实时荧光定量rt-pcr检测试剂盒 |
CN109762942A (zh) * | 2019-03-13 | 2019-05-17 | 中国疾病预防控制中心病毒病预防控制所 | 一种快速检测呼吸道合胞病毒的含内参双重等温核酸扩增方法 |
CN109762942B (zh) * | 2019-03-13 | 2022-05-17 | 中国疾病预防控制中心病毒病预防控制所 | 一种快速检测呼吸道合胞病毒的含内参双重等温核酸扩增方法 |
CN111778357A (zh) * | 2020-07-13 | 2020-10-16 | 国家卫生健康委科学技术研究所 | 基于CRISPR/Cas12a的呼吸道合胞病毒核酸快速检测试剂盒及其检测方法 |
Also Published As
Publication number | Publication date |
---|---|
CN104593524B (zh) | 2017-06-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Shen et al. | Recent advances and perspectives of nucleic acid detection for coronavirus | |
US20160289777A1 (en) | Primers and probes for detection and discrimination of types and subtypes of influenza viruses | |
CN102277455B (zh) | 猪圆环病毒、猪伪狂犬病毒和猪细小病毒检测用引物、探针及检测试剂盒 | |
CN104120080B (zh) | 一种α-珠蛋白基因突变检测试剂盒及其制备方法与应用 | |
CN104032034A (zh) | 一种同时检测甲型、乙型及丙型流感病毒的方法及试剂盒 | |
CN104195264A (zh) | 一种快速检测柯萨奇病毒a6型/a10型的核酸检测试剂盒及其应用 | |
CN104593524A (zh) | 一种快速检测呼吸道合胞病毒a型和b型的核酸检测试剂盒及其应用 | |
CN102876776B (zh) | 检测y染色体微缺失的实时荧光定量pcr试剂盒及方法 | |
US20220112567A1 (en) | Methods and compositions for influenza a virus subtyping | |
US20180265936A1 (en) | Compositions and methods for detection and discrimination of influenza viruses | |
CN103409552A (zh) | 同步检测多种高危型hpv基因型的引物组、探针组、方法及试剂盒 | |
CN103409554B (zh) | 一种快速检测麻疹病毒/风疹病毒的核酸检测试剂盒 | |
CN104878126A (zh) | 一种柯萨奇病毒a6型核酸的检测试剂盒及检测方法 | |
US10329630B2 (en) | Compositions and methods for detection and discrimination of emerging influenza virus subtypes | |
CN102286639A (zh) | 甲型h1n1/甲型流感病毒核酸双重荧光pcr检测试剂盒 | |
CN104419713A (zh) | 基于环介导的等温扩增的人呼吸道合胞病毒检测试剂盒 | |
CN105296675A (zh) | 一种乙型流感病毒核酸检测试剂盒及其制备方法 | |
CN103290141A (zh) | 16种高危型和5种低危型hpv检测试剂盒 | |
CN101586170B (zh) | 一种检测乙型肝炎病毒基因型的方法及试剂盒 | |
CN106319079A (zh) | 一种检测22q11.2拷贝数缺失的方法 | |
CN105695627A (zh) | 一种用于荧光定量pcr检测15型hpv的特异性引物和探针的组合以及试剂盒 | |
Hu et al. | Development of a real-time RT-PCR assay for detection and quantitation of parainfluenza virus 3 | |
EP4176089A2 (en) | Rapid detection kit for human pathogenic coronaviruses : betacoronavirus group b/c and sars-cov-2 | |
CN101195843A (zh) | 一种hbv dna基因分型荧光pcr多通道检测方法及其试剂盒 | |
CN114990261B (zh) | 用于检测呼吸道感染性疾病病原体的多重qPCR检测试剂 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: 225300, Jiangsu Taizhou Development Zone Temple Lane rich Nomura, Shuai village A (G19) third storey factory building and third, fourth floor research area Patentee after: Jiangsu world biological Polytron Technologies Inc Address before: 3 building, G19 building, No. 225300, drug City Avenue, Hailing District, Jiangsu, Taizhou Patentee before: Jiangsu Shuoshi Biological Technology Co., Ltd. |
|
CP02 | Change in the address of a patent holder | ||
CP02 | Change in the address of a patent holder |
Address after: No. 837, Yaocheng Avenue, Taizhou, Jiangsu 225300 Patentee after: JIANGSU BIOPERFECTUS TECHNOLOGIES CO.,LTD. Address before: 225300 Workshop on the third floor of building a (G19), Fuye village and shuaiyu village, Sixiang Development Zone, Taizhou City, Jiangsu Province, and office and research area on the third and fourth floors Patentee before: JIANGSU BIOPERFECTUS TECHNOLOGIES CO.,LTD. |