CN104542287B - A kind of preparation method of dendrobium candidum private organizations nutrient solution - Google Patents
A kind of preparation method of dendrobium candidum private organizations nutrient solution Download PDFInfo
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- 241000026010 Dendrobium candidum Species 0.000 title claims abstract description 33
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 68
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 45
- 238000003756 stirring Methods 0.000 claims abstract description 22
- 229920001817 Agar Polymers 0.000 claims abstract description 18
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 18
- 239000008272 agar Substances 0.000 claims abstract description 18
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 16
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims abstract description 16
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 14
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 14
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 14
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 11
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims abstract description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 9
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 9
- 235000020197 coconut milk Nutrition 0.000 claims abstract description 9
- 238000010411 cooking Methods 0.000 claims abstract description 9
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims abstract description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 9
- 235000013575 mashed potatoes Nutrition 0.000 claims abstract description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 9
- 235000012015 potatoes Nutrition 0.000 claims abstract description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 8
- 239000004471 Glycine Substances 0.000 claims abstract description 8
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000001110 calcium chloride Substances 0.000 claims abstract description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 8
- 235000011148 calcium chloride Nutrition 0.000 claims abstract description 8
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 claims abstract description 8
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 8
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims abstract description 8
- 229960000367 inositol Drugs 0.000 claims abstract description 8
- 235000007079 manganese sulphate Nutrition 0.000 claims abstract description 8
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 8
- 235000001968 nicotinic acid Nutrition 0.000 claims abstract description 8
- 229960003512 nicotinic acid Drugs 0.000 claims abstract description 8
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 8
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 8
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 8
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims abstract description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 8
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 8
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 8
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000004327 boric acid Substances 0.000 claims abstract description 7
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 7
- 235000015393 sodium molybdate Nutrition 0.000 claims abstract description 7
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 238000010438 heat treatment Methods 0.000 claims abstract description 6
- 235000019190 thiamine hydrochloride Nutrition 0.000 claims abstract description 6
- 229940099596 manganese sulfate Drugs 0.000 claims abstract description 4
- 239000011702 manganese sulphate Substances 0.000 claims abstract description 4
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims abstract description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims abstract description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 3
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims 2
- LFDGRWDETVOGDT-UHFFFAOYSA-N 1h-pyrrole;hydrochloride Chemical compound Cl.C=1C=CNC=1 LFDGRWDETVOGDT-UHFFFAOYSA-N 0.000 claims 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims 1
- 206010044565 Tremor Diseases 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 abstract description 3
- 229960000344 thiamine hydrochloride Drugs 0.000 abstract description 3
- 239000011747 thiamine hydrochloride Substances 0.000 abstract description 3
- 229960002645 boric acid Drugs 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 238000012856 packing Methods 0.000 abstract 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract 1
- 240000004638 Dendrobium nobile Species 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 150000003840 hydrochlorides Chemical class 0.000 description 4
- 241001523681 Dendrobium Species 0.000 description 3
- -1 lipid peroxide Chemical class 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000015961 tonic Nutrition 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241000737241 Cocos Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- QSNCUGULHPBRGR-UHFFFAOYSA-N Dendramine Natural products CC12C3(O)CCC2CN(C)C1C1C(C(C)C)C3C(=O)O1 QSNCUGULHPBRGR-UHFFFAOYSA-N 0.000 description 1
- RYAHJFGVOCZDEI-UFFNCVEVSA-N Dendrobine Chemical compound C([C@H]1CC[C@@H]2[C@@]31C)N(C)[C@@H]3[C@H]1[C@@H](C(C)C)[C@@H]2C(=O)O1 RYAHJFGVOCZDEI-UFFNCVEVSA-N 0.000 description 1
- 241001076416 Dendrobium tosaense Species 0.000 description 1
- 241000594394 Hedyotis Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- KTHDTJVBEPMMGL-VKHMYHEASA-N N-acetyl-L-alanine Chemical compound OC(=O)[C@H](C)NC(C)=O KTHDTJVBEPMMGL-VKHMYHEASA-N 0.000 description 1
- MAEQVSBPCIMMJF-UHFFFAOYSA-N Nobilonin Natural products CC(C)C1C2C(=O)C3(C)C(CN(C)C)CCC3C1C(=O)O2 MAEQVSBPCIMMJF-UHFFFAOYSA-N 0.000 description 1
- MAEQVSBPCIMMJF-MSZYXCAISA-N Nobilonine Natural products O=C1[C@@]2(C)[C@@H](CN(C)C)CC[C@H]2[C@H]2[C@H](C(C)C)[C@H]1OC2=O MAEQVSBPCIMMJF-MSZYXCAISA-N 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- YWAAZZCPWLHJAN-UHFFFAOYSA-N Shihunine Chemical compound CN1CCCC11C2=CC=CC=C2C(=O)O1 YWAAZZCPWLHJAN-UHFFFAOYSA-N 0.000 description 1
- YWAAZZCPWLHJAN-GFCCVEGCSA-N Shihunine Natural products O=C1O[C@@]2(N(C)CCC2)c2c1cccc2 YWAAZZCPWLHJAN-GFCCVEGCSA-N 0.000 description 1
- 240000001519 Verbena officinalis Species 0.000 description 1
- 235000018718 Verbena officinalis Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- RYAHJFGVOCZDEI-CZKZLRAZSA-N dendrobine Natural products O=C1O[C@@H]2[C@H](C(C)C)[C@H]1[C@H]1[C@@]3(C)[C@@H]2N(C)C[C@H]3CC1 RYAHJFGVOCZDEI-CZKZLRAZSA-N 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- IRPDISVJRAYFBI-UHFFFAOYSA-N nitric acid;potassium Chemical compound [K].O[N+]([O-])=O IRPDISVJRAYFBI-UHFFFAOYSA-N 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229950001390 sudismase Drugs 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Abstract
The invention discloses a kind of preparation method of dendrobium candidum private organizations nutrient solution, including：KI, manganese sulfate, zinc sulfate, boric acid, sodium molybdate, copper sulphate, cobalt chloride are dissolved to obtain the first component；Ferrous sulfate and disodium ethylene diamine tetraacetate are dissolved to obtain the second component；Inositol, nicotinic acid, puridoxine hydrochloride, thiamine hydrochloride and glycine are dissolved to obtain third component；Peeled potatoes are weighed, is added in cooking machine, is stirred to obtain mashed potatoes, adds coconut milk, material A is obtained after well mixed；Ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, potassium dihydrogen phosphate are added to the water dissolving and obtain material B；Then material A is heated, material B is added in heating process, the first component, the second component and third component are sequentially added after stirring, brown sugar and agar is continuously added and stirs, mixed material is obtained after adjusting pH value；Autoclave sterilization is carried out after packing, room temperature is cooled to and obtains dendrobium candidum private organizations nutrient solution.
Description
Technical field
The present invention relates to technical field of plant culture, more particularly to a kind of preparation side of dendrobium candidum private organizations nutrient solution
Method
Background technology
The stem of noble dendrobium is that orchid family Dendrobium grows nonparasitically upon another plant herbaceous plant for many years, is the conventional rare traditional Chinese medicine of China's tradition.Early in《The legendary god of farming
Book on Chinese herbal medicine warp》With《Compendium of Materia Medica》On be classified as top grade medicinal material, history tree and current《Pharmacopoeia of People's Republic of China》In have receipts
Carry.The stem of noble dendrobium is used as medicine with stem, contains a variety of alkaloids such as dendrobine, dendramine, nobilonine, shihunine；Slightly sweet flavor, cold nature；
There are tonifying-Yin and nourishing-stomach, clearing heat and promoting fluid effect, mainly there is bright treatment to diseases such as digestive system, respiratory system, angiocarpy and ophthalmology
Effect.Modern medicine proves that the stem of noble dendrobium can stimulate circulation, and strengthens cardiac function；Promote enterogastric peristalsis, strengthen digestive function；
The SOP (Sudismase) that can be significantly improved in human body is horizontal, reduces LPO (lipid peroxide), plays significant anti-aging and makees
With.
Dendrobium candidum is also known as ribbed hedyotis herb, belongs to orchid family Dendrobium, is the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family, is a kind of precious
Wild Medicinal.Height above sea level is born in up on the dark and damp rock in 1600 meters of mountain region half, liking warm and moist weather and half cloudy half light
It environment, can not resist cold, be generally resistant to -5 DEG C of low temperature.The stem of noble dendrobium can be divided into tens of kinds of yellow grass, Jin Chai, horsewhip etc., dendrobium candidum and
HERBA DENDROBII is the superfine product of the stem of noble dendrobium, and dendrobium candidum is listed in " first of Chinese nine big celestial grass ", since the Tang and Song Dynasty, has been listed in emperor always
Room tribute, dendrobium candidum are gained the name because of epidermis in iron green.Dendrobium candidum has unique medical value, is used as medicine with its stem, in
Medicine name：The stem of noble dendrobium, belong to the yin tonics in tonic,《Chinese Pharmacopoeia》：Reinforcing stomach reg fluid, nourishing Yin and clearing heat.A small number of kinds such as dendrobium candidum
Tender stem, be twisted into helical form or spring-like, dry, commodity are referred to as earrings dendrobium, however, because growth cycle is slow, it is extremely difficult to survive,
Dendrobium officinale is endangered, artificial breeding, and survival rate is relatively low.
The content of the invention
Based on technical problem existing for background technology, the present invention proposes a kind of system of dendrobium candidum private organizations nutrient solution
Preparation Method, by adjusting the ratio between a component and the sequencing of addition, make the nutrient solution finally given, can be iron sheet
The stem of noble dendrobium provides comprehensive nutriment, can make the quick promoting root growth life stem of dendrobium candidum, and can improve polysaccharide in dendrobium candidum and contain
Amount, amino acid content.
A kind of preparation method of dendrobium candidum private organizations nutrient solution proposed by the present invention, comprises the following steps：
S1,2.2-2.5 parts KI, 40-42 parts manganese sulfate, 23-25 parts zinc sulfate, 70-72 part boron are weighed by molar part
Acid, 1-1.5 parts sodium molybdate, 0.1-0.3 parts copper sulphate, 0.3-0.5 part cobalt chlorides, are uniformly mixing to obtain first group after being dissolved in water
Point, wherein the molal volume of KI and the first component ratio (mmol/L) is 2.2-2.5：2.3-2.4；
S2,30-33 parts ferrous sulfate and 59-62 part disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water
Mix and uniformly obtain the second component, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 30-33：1.9-2.1；
S3,1.4-1.6 × 10 are weighed by weight5Part inositol, 400-410 parts nicotinic acid, 780-800 parts puridoxine hydrochloride,
170-190 parts thiamine hydrochloride and 1550-1600 part glycine, are uniformly mixing to obtain third component, wherein salt after being dissolved in water
Sour pyridoxol and the mass volume ratio of third component (g/L) are 156-160：1000；
S4,3800-4000 part peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain soil after adding water
Beans mud, 1000-1200 part coconut milk is added, material A is obtained after well mixed；By 57-60 parts ammonium nitrate, 67-70 part nitric acid
Potassium, 16-20 parts calcium chloride, 14-17 parts magnesium sulfate, 7-10 part potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material
B；Then material A is heated, in heating process add material B, sequentially added after stirring the first component, the second component and
Third component simultaneously stirs, and continuously adds 3000-3200 parts brown sugar and 300-320 parts agar and stirs, is dissolved in water
After stir after, regulation pH value be 5.5-6 obtain the mass volume ratio (g/L) of mixed material, wherein agar and mixed material
For 75-80：17-18, the first component, the second component, the volume ratio of third component and mixed material are 0.2-0.4:0.2-0.4:
0.2-0.4:65-75；
S5, after mixed material is dispensed, autoclave sterilization is carried out, be cooled to room temperature and obtain the training of dendrobium candidum private organizations
Nutrient solution.
Preferably, in S1,2.3-2.4 parts KI, 40.6-41.2 parts manganese sulfate, 23.7-24.5 parts are weighed by molar part
Zinc sulfate, 70.8-71.2 parts boric acid, 1.2-1.4 parts sodium molybdate, 0.13-0.22 parts copper sulphate, 0.36-0.45 part cobalt chlorides, add
The first component is uniformly mixing to obtain after water dissolving, the wherein molal volume of KI and the first component ratio (mmol/L) is 2.3-
2.4：2.35-2.37.
Preferably, in S2, the mol ratio of ferrous sulfate and disodium ethylene diamine tetraacetate is 31-32：60-61.
Preferably, in S3,1.45-1.56 × 10 are weighed by weight5Part inositol, 403-407 parts nicotinic acid, 785-795 parts
Puridoxine hydrochloride, 175-185 parts thiamine hydrochloride and 1580-1590 part glycine, the 3rd is uniformly mixing to obtain after being dissolved in water
The mass volume ratio (g/L) of component, wherein puridoxine hydrochloride and third component is 157-159：1000；
Preferably, in S4,3850-3900 part peeled potatoes are weighed by weight, are added in cooking machine, are stirred after adding water
Mix to obtain mashed potatoes, add 1050-1150 part coconut milk, material A is obtained after well mixed；By 58-59 parts ammonium nitrate, 68-
69 parts of potassium nitrate, 17-19 parts calcium chloride, 15-16 parts magnesium sulfate, 8-9 part potassium dihydrogen phosphates are added to the water dissolving and mixing
Obtain material B；Then material A is heated, material B is added in heating process, the first component, the are sequentially added after stirring
Two components and third component simultaneously stir, and continuously add 3100-3150 parts brown sugar and 305-315 parts agar and stir,
After being stirred after being dissolved in water, regulation pH value is that 5.5-6 obtains the quality volume of mixed material, wherein agar and mixed material
It is 77-78 than (g/L)：17-18, the first component, the second component, the volume ratio of third component and mixed material are 0.3:0.3:
0.3:68-72。
Compatibility of the present invention is reasonable, by adjusting the ratio between a component and the sequencing of addition, makes N P and K etc. normal
Secondary element and trace element make the nutrient solution finally given, can provided for dendrobium candidum entirely among rational ratio
The nutriment in face, and organic substance and inorganic salts cooperate, and can make the quick promoting root growth life stem of dendrobium candidum, mashed potatoes, coconut palm
Sub- juice, brown sugar and agar cooperate, it is possible to increase polyoses content, amino acid content in dendrobium candidum, improve dendrobium candidum
Rooting rate and the speed of growth, significantly improve the survival rate of dendrobium candidum.
Embodiment
Below, technical scheme is described in detail by specific embodiment.
Embodiment 1
A kind of preparation method of dendrobium candidum private organizations nutrient solution proposed by the present invention, comprises the following steps：
S1, weigh by molar part 2.2 parts of KIs, 42 parts of manganese sulfates, 23 parts of zinc sulfate, 72 parts of boric acid, 1 part of sodium molybdate,
0.3 part of copper sulphate, 0.3 part of cobalt chloride, be uniformly mixing to obtain the first component after being dissolved in water, wherein KI and the first component
Molal volume ratio (mmol/L) is 25：23；
S2,33 parts of ferrous sulfate and 59 parts of disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water
The second component is obtained, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 33：1.9；
S3,1.6 × 10 are weighed by weight5Part inositol, 400 parts of nicotinic acid, 800 parts of puridoxine hydrochlorides, 170 parts of thiamine hydrochlorides
Element and 1600 parts of glycine, the matter of third component, wherein puridoxine hydrochloride and third component is uniformly mixing to obtain after being dissolved in water
It is 156 to measure volume ratio (g/L)：1000；
S4,4000 parts of peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain mashed potatoes after adding water,
1000 portions of coconut milk are added, material A is obtained after well mixed；By 60 parts of ammonium nitrate, 67 parts of potassium nitrate, 20 parts of calcium chloride, 14
Part magnesium sulfate, 10 parts of potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material B；Then material A is heated, heated
During add material B, the first component, the second component and third component are sequentially added after stirring and is stirred, continue
Add 3000 portions of brown sugar and 320 parts of agar and stir, after being stirred after being dissolved in water, regulation pH value is mixed for 5.5
The mass volume ratio (g/L) of compound material, wherein agar and mixed material is 80：17, the first component, the second component, third component
Volume ratio with mixed material is 0.4:0.2:0.4:65；
S5, after mixed material is dispensed, autoclave sterilization is carried out, be cooled to room temperature and obtain the training of dendrobium candidum private organizations
Nutrient solution.
Embodiment 2
A kind of preparation method of dendrobium candidum private organizations nutrient solution proposed by the present invention, comprises the following steps：
S1,2.5 parts of KIs, 40 parts of manganese sulfates, 25 parts of zinc sulfate, 70 parts of boric acid, 1.5 parts of molybdic acids are weighed by molar part
Sodium, 0.1 part of copper sulphate, 0.5 part of cobalt chloride, are uniformly mixing to obtain the first component after being dissolved in water, wherein KI and first group
The molal volume ratio (mmol/L) divided is 11：12；
S2,30 parts of ferrous sulfate and 62 parts of disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water
The second component is obtained, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 10：0.7；
S3,1.4 × 10 are weighed by weight5Part inositol, 410 parts of nicotinic acid, 780 parts of puridoxine hydrochlorides, 190 parts of thiamine hydrochlorides
Element and 1550 parts of glycine, the matter of third component, wherein puridoxine hydrochloride and third component is uniformly mixing to obtain after being dissolved in water
It is 16 to measure volume ratio (g/L)：100；
S4,3800 parts of peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain mashed potatoes after adding water,
1200 portions of coconut milk are added, material A is obtained after well mixed；By 57 parts of ammonium nitrate, 70 parts of potassium nitrate, 16 parts of calcium chloride, 17
Part magnesium sulfate, 7 parts of potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material B；Then material A is heated, heated
During add material B, the first component, the second component and third component are sequentially added after stirring and is stirred, continue
Add 3200 portions of brown sugar and 300 parts of agar and stir, after being stirred after being dissolved in water, regulation pH value is mixed for 6
The mass volume ratio (g/L) of material, wherein agar and mixed material is 75：18, the first component, the second component, third component and
The volume ratio of mixed material is 0.2:0.4:0.2:75；
S5, after mixed material is dispensed, autoclave sterilization is carried out, be cooled to room temperature and obtain the training of dendrobium candidum private organizations
Nutrient solution.
Embodiment 3
A kind of preparation method of dendrobium candidum private organizations nutrient solution proposed by the present invention, comprises the following steps：
S1, weigh by molar part 2.3 parts of KIs, 41.2 parts of manganese sulfates, 23.7 parts of zinc sulfate, 71.2 parts of boric acid, 1.2 parts
Sodium molybdate, 0.22 part of copper sulphate, 0.36 part of cobalt chloride, are uniformly mixing to obtain the first component after being dissolved in water, wherein KI with
The molal volume ratio (mmol/L) of first component is 2.4：2.35；
S2,32 parts of ferrous sulfate and 60 parts of disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water
The second component is obtained, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 32：1.9；
S3,1.56 × 10 are weighed by weight5Part inositol, 403 parts of nicotinic acid, 795 parts of puridoxine hydrochlorides, 175 parts of hydrochloric acid sulphur
Amine element and 1590 parts of glycine, be uniformly mixing to obtain third component after being dissolved in water, wherein puridoxine hydrochloride and third component
Mass volume ratio (g/L) is 157：1000；
S4,3900 parts of peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain mashed potatoes after adding water,
1050 portions of coconut milk are added, material A is obtained after well mixed；By 59 parts of ammonium nitrate, 68 parts of potassium nitrate, 19 parts of calcium chloride, 15
Part magnesium sulfate, 9 parts of potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material B；Then material A is heated, heated
During add material B, the first component, the second component and third component are sequentially added after stirring and is stirred, continue
Add 3100 portions of brown sugar and 315 parts of agar and stir, after being stirred after being dissolved in water, regulation pH value is mixed for 5.5
The mass volume ratio (g/L) of compound material, wherein agar and mixed material is 78：17, the first component, the second component, third component
Volume ratio with mixed material is 1:1:1:240；
S5, after mixed material is dispensed, autoclave sterilization is carried out, be cooled to room temperature and obtain the training of dendrobium candidum private organizations
Nutrient solution.
Embodiment 4
A kind of preparation method of dendrobium candidum private organizations nutrient solution proposed by the present invention, comprises the following steps：
S1, weigh by molar part 2.4 parts of KIs, 40.6 parts of manganese sulfates, 24.5 parts of zinc sulfate, 70.8 parts of boric acid, 1.4 parts
Sodium molybdate, 0.13 part of copper sulphate, 0.45 part of cobalt chloride, are uniformly mixing to obtain the first component after being dissolved in water, wherein KI with
The molal volume ratio (mmol/L) of first component is 2.3：2.37；
S2,31 parts of ferrous sulfate and 61 parts of disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water
The second component is obtained, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 31：2.1；
S3,1.45 × 10 are weighed by weight5Part inositol, 407 parts of nicotinic acid, 785 parts of puridoxine hydrochlorides, 185 parts of hydrochloric acid sulphur
Amine element and 1580 parts of glycine, be uniformly mixing to obtain third component after being dissolved in water, wherein puridoxine hydrochloride and third component
Mass volume ratio (g/L) is 159：1000；
S4,3850 parts of peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain mashed potatoes after adding water,
1150 portions of coconut milk are added, material A is obtained after well mixed；By 58 parts of ammonium nitrate, 69 parts of potassium nitrate, 17 parts of calcium chloride, 16
Part magnesium sulfate, 8 parts of potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material B；Then material A is heated, heated
During add material B, the first component, the second component and third component are sequentially added after stirring and is stirred, continue
Add 3150 portions of brown sugar and 305 parts of agar and stir, after being stirred after being dissolved in water, regulation pH value is mixed for 6
The mass volume ratio (g/L) of material, wherein agar and mixed material is 77：18, the first component, the second component, third component and
The volume ratio of mixed material is 0.3:0.3:0.3:68；
S5, after mixed material is dispensed, autoclave sterilization is carried out, be cooled to room temperature and obtain the training of dendrobium candidum private organizations
Nutrient solution.
It should be noted that in the present invention, the order of tri- steps of S1, S2 and S3 is not defined, Ke Yigen
The order of above three step is adjusted according to the convenience of arrangement of time, those skilled in the art are not paying creative labor
On the basis of dynamic, the arrangement to above three step adjusts obtained technical scheme, all should be within protection scope of the present invention.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (3)
1. a kind of preparation method of dendrobium candidum private organizations nutrient solution, it is characterised in that comprise the following steps：
S1,2.3-2.4 parts KI, 40.6-41.2 parts manganese sulfate, 23.7-24.5 parts zinc sulfate, 70.8- are weighed by molar part
71.2 parts of boric acid, 1.2-1.4 parts sodium molybdate, 0.13-0.22 parts copper sulphate, 0.36-0.45 part cobalt chlorides, are stirred after being dissolved in water
The first component uniformly is obtained, the wherein molal volume of KI and the first component ratio (mmol/L) is 2.3-2.4：2.35-2.37；
S2,30-33 parts ferrous sulfate and 59-62 part disodium ethylene diamine tetraacetates are weighed by molar part, stirred after being dissolved in water equal
Even to obtain the second component, the wherein molal volume of ferrous sulfate and the second component ratio (mmol/L) is 30-33：1.9-2.1；
S3,1.45-1.56 × 10 are weighed by weight5Part inositol, 403-407 parts nicotinic acid, 785-795 parts puridoxine hydrochloride, 175-
185 parts of thiamine hydrochlorides and 1580-1590 part glycine, are uniformly mixing to obtain third component, wherein hydrochloric acid pyrrole after being dissolved in water
The mass volume ratio (g/L) for alcohol and the third component of trembling is 157-159：1000；
S4,3800-4000 part peeled potatoes are weighed by weight, add in cooking machine, be stirred to obtain mashed potatoes after adding water,
1000-1200 part coconut milk is added, material A is obtained after well mixed；By 57-60 parts ammonium nitrate, 67-70 parts potassium nitrate, 16-
20 parts of calcium chloride, 14-17 parts magnesium sulfate, 7-10 part potassium dihydrogen phosphates are added to the water dissolving and mixing and obtain material B；Then
Material A is heated, material B is added in heating process, the first component, the second component and the 3rd group are sequentially added after stirring
Divide and stir, continuously add 3000-3200 parts brown sugar and 300-320 parts agar and stir, stirred after being dissolved in water
After uniformly, regulation pH value is that 5.5-6 obtains mixed material, and the mass volume ratio (g/L) of wherein agar and mixed material is 75-
80：17-18, the first component, the second component, the volume ratio of third component and mixed material are 0.2-0.4:0.2-0.4:0.2-
0.4:65-75；
S5, after mixed material is dispensed, autoclave sterilization is carried out, room temperature is cooled to and obtains dendrobium candidum private organizations culture
Liquid.
2. the preparation method of dendrobium candidum private organizations nutrient solution according to claim 1, it is characterised in that in S2, sulfuric acid
Ferrous and disodium ethylene diamine tetraacetate mol ratio is 31-32：60-61.
3. the preparation method of dendrobium candidum private organizations nutrient solution according to claim 1 or claim 2, it is characterised in that in S4, press
Parts by weight weigh 3850-3900 part peeled potatoes, add in cooking machine, are stirred to obtain mashed potatoes after adding water, add
1050-1150 part coconut milk, material A is obtained after well mixed；By 58-59 parts ammonium nitrate, 68-69 parts potassium nitrate, 17-19 part chlorine
Change calcium, 15-16 parts magnesium sulfate, 8-9 part potassium dihydrogen phosphates to be added to the water dissolving and mixing and obtain material B；Then by material A
Heating, material B is added in heating process, the first component, the second component and third component are sequentially added after stirring and is stirred
Mix uniformly, continuously add 3100-3150 parts brown sugar and 305-315 parts agar and stir, after being stirred after being dissolved in water,
Regulation pH value is that 5.5-6 obtains mixed material, and the mass volume ratio (g/L) of wherein agar and mixed material is 77-78：17-18,
First component, the second component, the volume ratio of third component and mixed material are 0.3:0.3:0.3:68-72.
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| CN107354095A (en) * | 2017-03-20 | 2017-11-17 | 北京君立康生物科技有限公司 | Culture medium, culture media composition and preparation method thereof |
| CN109548625A (en) * | 2018-12-28 | 2019-04-02 | 苏州天成新农生物科技有限公司 | Fluid nutrient medium and preparation method thereof suitable for dendrobium candidum tender stem |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102976848A (en) * | 2012-11-30 | 2013-03-20 | 富阳市文曲生态农业开发有限公司 | Dendrobium officinale culture solution |
| CN103891615A (en) * | 2014-03-27 | 2014-07-02 | 西南林业大学 | Culture medium capable of strengthening seedlings and growing roots for dendrobium |
| CN103931496A (en) * | 2014-04-02 | 2014-07-23 | 河南省南阳农业学校 | Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method |
| CN104082150A (en) * | 2014-07-31 | 2014-10-08 | 张松波 | Culture medium for dendrobium officinale |
| CN104206282A (en) * | 2014-10-10 | 2014-12-17 | 江苏神草生物科技有限公司 | Culture medium for dendrobium tissue cultivation |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102976848A (en) * | 2012-11-30 | 2013-03-20 | 富阳市文曲生态农业开发有限公司 | Dendrobium officinale culture solution |
| CN103891615A (en) * | 2014-03-27 | 2014-07-02 | 西南林业大学 | Culture medium capable of strengthening seedlings and growing roots for dendrobium |
| CN103931496A (en) * | 2014-04-02 | 2014-07-23 | 河南省南阳农业学校 | Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method |
| CN104082150A (en) * | 2014-07-31 | 2014-10-08 | 张松波 | Culture medium for dendrobium officinale |
| CN104206282A (en) * | 2014-10-10 | 2014-12-17 | 江苏神草生物科技有限公司 | Culture medium for dendrobium tissue cultivation |
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