CN104082150A - Culture medium for dendrobium officinale - Google Patents
Culture medium for dendrobium officinale Download PDFInfo
- Publication number
- CN104082150A CN104082150A CN201410373771.5A CN201410373771A CN104082150A CN 104082150 A CN104082150 A CN 104082150A CN 201410373771 A CN201410373771 A CN 201410373771A CN 104082150 A CN104082150 A CN 104082150A
- Authority
- CN
- China
- Prior art keywords
- parts
- dendrobium officinale
- culture medium
- medium
- following weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a culture medium for dendrobium officinale. The culture medium consists of the following components in parts by weight: 10-15 parts of activated carbon, 5-10 parts of saw dust, 3-8 parts of sweet potato puree, 1-3 parts of naphthalene acetic acid and 1-3 parts of microelement addition solution, wherein the microelement addition solution contains KI, MnSO4.H2O, H3BO3, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O and CoCl2.6H2O. The culture medium is rich in various nutrient substances and plant hormones needed by rooting growth of the dendrobium officinale. Extremely high rooting rate of the dendrobium officinale and good growth of rootstalks can be ensured, and basic guarantee is provided for further transplantation and survival of tissue culture seedlings of the dendrobium officinale.
Description
Technical field
The present invention relates to a kind of plant culture, be specifically related to a kind of medium of dendrobium candidum.
Background technology
Dendrobium candidum is the orchid family (Orchidaceae) epiphyte, is mainly distributed in the ground such as Jiangxi, Guangxi, Guangdong, Guizhou, Yunnan, Anhui, Zhejiang, Hunan, Shaanxi, Henan, Fujian.Its habitat is unique, microclimate is required very strict, often grows between height above sea level 500～1500m, grow nonparasitically upon another plant on overhanging cliff or the trunk of broad-leaved tree on and on limestone, like environment warm, moistening, half light.The selection of medium is the tame crucial step of dendrobium candidum, only has and selects suitable medium just can guarantee that dendrobium candidum takes root, grow soon.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of medium of dendrobium candidum, to improve the good growth of rooting rate and the rhizome of dendrobium candidum.
For addressing the above problem, the present invention will be by the following technical solutions:
A medium for dendrobium candidum, consists of the component of following weight portion: 10～15 parts of active carbons, 5～10 parts of wood chips, 3～8 parts, Ipomoea batatas mud, 1～3 part of methyl α-naphthyl acetate, 1～3 part of micro-annex solution;
Wherein, the composition that contains following weight in every L trace element annex solution:
In such scheme, the best composition weight portion of described medium is: 13 parts of active carbons, 8 parts of wood chips, 5 parts, Ipomoea batatas mud, 5 parts of methyl α-naphthyl acetates, 5 parts of micro-annex solutions.
The optimum weight content that contains composition in described every L trace element annex solution is:
Beneficial effect of the present invention is:
The present invention is by choosing rational culture medium prescription, be rich in dendrobium candidum required various nutriments and the plant hormone of growing up of taking root, can guarantee rooting rate that dendrobium candidum is high and the good growth of rhizome, next step candidum tissue culturing seedling transplant survival provides basic guarantee.
Embodiment
With specific embodiment, be further described below, but the present invention is not limited to these embodiment.
Embodiment 1
The medium of dendrobium candidum, consists of the component of following weight portion: 13 parts of active carbons, 8 parts of wood chips, 5 parts, Ipomoea batatas mud, 5 parts of methyl α-naphthyl acetates, 5 parts of micro-annex solutions;
Wherein, the composition that contains following weight in every L trace element annex solution:
Embodiment 2
The medium of dendrobium candidum, consists of the component of following weight portion: 10 parts of active carbons, 8 parts of wood chips, 8 parts, Ipomoea batatas mud, 1 part of methyl α-naphthyl acetate, 2 parts of micro-annex solutions;
Wherein, the composition that contains following weight in every L trace element annex solution:
Embodiment 3
The medium of dendrobium candidum, consists of the component of following weight portion: 13 parts of active carbons, 10 parts of wood chips, 3 parts, Ipomoea batatas mud, 2 parts of methyl α-naphthyl acetates, 3 parts of micro-annex solutions;
Wherein, the composition that contains following weight in every L trace element annex solution:
Embodiment 4
The medium of dendrobium candidum, consists of the component of following weight portion: 15 parts of active carbons, 5 parts of wood chips, 6 parts, Ipomoea batatas mud, 3 parts of methyl α-naphthyl acetates, 1 part of micro-annex solution;
Wherein, the composition that contains following weight in every L trace element annex solution:
Experimental example
Selection height of seedling is the seedlings of Dendrobium officinale of 4～5 of 2～3cm, the number of blade, discards yellow and hypogenetic plant, accesses medium in embodiment 1～4 under aseptic condition, take ordinary culture medium as control group.Each processes 10 bottles of inoculations, 10 every bottle, cultivation temperature (25 ± 1) ℃, intensity of illumination 2000～2500Ix, light application time 12h/d.After 60d, observe statistics stem of noble dendrobium seedling rooting situation, situation is as shown in the table:
| Process | Rooting rate (%) | Mean elements (bar) | Root growth situation |
| Ordinary culture medium | 63 | 2.5 | Root system is more strong, and seedling growth is slow, has Huang Ye |
| Embodiment 1 | 100 | 4.5 | Root system is strong, and seedling growth is fast, sturdy, without yellow leaf |
| Embodiment 2 | 97 | 3.8 | Root system is strong, and seedling growth is very fast, without yellow leaf |
| Embodiment 3 | 92 | 3.2 | Root system is strong, and seedling growth is very fast, without yellow leaf |
| Embodiment 4 | 95 | 3.4 | Root system is strong, and seedling growth is very fast, without yellow leaf |
Claims (3)
1. a medium for dendrobium candidum, is characterized in that, consists of: 10～15 parts of active carbons, 5～10 parts of wood chips, 3～8 parts, Ipomoea batatas mud, 1～3 part of methyl α-naphthyl acetate, 1～3 part of micro-annex solution the component of following weight portion;
Wherein, the composition that contains following weight in every L trace element annex solution:
2. medium according to claim 1, is characterized in that, the ingredients weight parts of described medium is: 13 parts of active carbons, 8 parts of wood chips, 5 parts, Ipomoea batatas mud, 5 parts of methyl α-naphthyl acetates, 5 parts of micro-annex solutions.
3. medium according to claim 1 and 2, is characterized in that, the composition that contains following weight in every L trace element annex solution:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410373771.5A CN104082150A (en) | 2014-07-31 | 2014-07-31 | Culture medium for dendrobium officinale |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410373771.5A CN104082150A (en) | 2014-07-31 | 2014-07-31 | Culture medium for dendrobium officinale |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104082150A true CN104082150A (en) | 2014-10-08 |
Family
ID=51630003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410373771.5A Pending CN104082150A (en) | 2014-07-31 | 2014-07-31 | Culture medium for dendrobium officinale |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104082150A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542287A (en) * | 2014-12-31 | 2015-04-29 | 安徽康久生物科技有限公司 | Preparation method of special tissue culture solution for dendrobium candidum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889547A (en) * | 2009-05-22 | 2010-11-24 | 云南省德宏热带农业科学研究所 | Aseptic and rapid propagation method of dendrobium devonianum seeds |
| CN103120123A (en) * | 2013-02-04 | 2013-05-29 | 四川深达生物科技有限公司 | Culture medium for improving dendrobium officinale maturing rate and method for preparing culture medium |
| CN103210842A (en) * | 2013-02-04 | 2013-07-24 | 四川深达生物科技有限公司 | Dendrobium candidum test-tube flowering and seed setting method |
| WO2015003408A1 (en) * | 2013-07-08 | 2015-01-15 | 中国科学院华南植物园 | Dendrobium in vitro crossbreeding method |
-
2014
- 2014-07-31 CN CN201410373771.5A patent/CN104082150A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889547A (en) * | 2009-05-22 | 2010-11-24 | 云南省德宏热带农业科学研究所 | Aseptic and rapid propagation method of dendrobium devonianum seeds |
| CN103120123A (en) * | 2013-02-04 | 2013-05-29 | 四川深达生物科技有限公司 | Culture medium for improving dendrobium officinale maturing rate and method for preparing culture medium |
| CN103210842A (en) * | 2013-02-04 | 2013-07-24 | 四川深达生物科技有限公司 | Dendrobium candidum test-tube flowering and seed setting method |
| WO2015003408A1 (en) * | 2013-07-08 | 2015-01-15 | 中国科学院华南植物园 | Dendrobium in vitro crossbreeding method |
Non-Patent Citations (1)
| Title |
|---|
| 付传明等: ""铁皮石斛无菌播种产业化繁育技术研究"", 《广西植物》, vol. 32, no. 2, 31 March 2012 (2012-03-31) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542287A (en) * | 2014-12-31 | 2015-04-29 | 安徽康久生物科技有限公司 | Preparation method of special tissue culture solution for dendrobium candidum |
| CN104542287B (en) * | 2014-12-31 | 2017-11-21 | 蒙城县望槐信息科技有限责任公司 | A kind of preparation method of dendrobium candidum private organizations nutrient solution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103053397B (en) | Micro-potato production method by mist culture method | |
| CN104710230A (en) | Seedling cultivation matrix for organic production of greenhouse solanaceous and melon vegetables | |
| CN107810857A (en) | A kind of detoxicated ginger method for tissue culture | |
| CN104620859A (en) | Method for cultivating southern ramulus mori mushroom | |
| CN105254412B (en) | A kind of diatomite-charcoal matrix for officinal dendrobium stem plantation | |
| CN106472274A (en) | Special soilless culture nutrient fluid of a kind of Chinese cabbage and preparation method thereof | |
| CN107912230A (en) | A kind of selenium-rich hickory chick culture substrate | |
| CN104160958A (en) | Test-tube cassava induction method | |
| CN105379552A (en) | Seedling method for asparagus | |
| CN102577917A (en) | Nutrient water-saving substrate special for soilless raising of tomato seedlings | |
| CN104082150A (en) | Culture medium for dendrobium officinale | |
| CN104025894A (en) | Cultivating method of vine tea seedling | |
| CN202759888U (en) | Fruit tree seedling box | |
| CN106613805A (en) | Tea tree plant-pit seedling culture matrix | |
| CN105284572A (en) | Rose tissue culture seedling transplanting method and matrix thereof | |
| CN103535373B (en) | A kind of production method of low hormone residues muskmelon | |
| CN103238640A (en) | Plant regulating agent for growth of dendrobium-candidum seedlings | |
| CN105753577A (en) | Potato planting nutrient solution | |
| CN103229700A (en) | Substrate for dendrobium officinale tissue culture seedlings to survive and grow | |
| CN101638330A (en) | High-efficiency compound flower fertilizer and preparation method thereof | |
| CN104584852B (en) | A kind of method that Herba Loti Tenuis breeding adds generation | |
| CN104671893A (en) | Preparation method for watermelon biological organic fertilizer | |
| CN108094124A (en) | A kind of preparation method of flowers environment-friendly substrate | |
| CN103641596B (en) | A kind of Potmarigold Calendula special fertilizer | |
| CN107500859A (en) | A kind of Chinese cabbage takes root nutrient solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20141008 |