CN104540965B - 靶标检测和信号放大 - Google Patents

靶标检测和信号放大 Download PDF

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Publication number
CN104540965B
CN104540965B CN201380042575.3A CN201380042575A CN104540965B CN 104540965 B CN104540965 B CN 104540965B CN 201380042575 A CN201380042575 A CN 201380042575A CN 104540965 B CN104540965 B CN 104540965B
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China
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substrate
catalytic
segment
nuclease
oligonucleotide
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Expired - Fee Related
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English (en)
Chinese (zh)
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CN104540965A (zh
Inventor
西蒙·马克·伯恩
艾莉森·威尔彦·托德
蒂莫西·丹尼尔·米罕
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SpeeDx Pty Ltd
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SpeeDx Pty Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9005—Enzymes with nucleic acid structure; e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380042575.3A 2012-06-18 2013-06-18 靶标检测和信号放大 Expired - Fee Related CN104540965B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
AU2012902551 2012-06-18
AU2012902551A AU2012902551A0 (en) 2012-06-18 Target detection and signal amplification
AU2012903462 2012-08-10
AU2012903462A AU2012903462A0 (en) 2012-08-10 Target detection and signal amplification
AU2013202354A AU2013202354A1 (en) 2012-06-18 2013-04-03 Target detection and signal amplification
AU2013202354 2013-04-03
PCT/AU2013/000651 WO2013188912A1 (en) 2012-06-18 2013-06-18 Target detection and signal amplification

Publications (2)

Publication Number Publication Date
CN104540965A CN104540965A (zh) 2015-04-22
CN104540965B true CN104540965B (zh) 2019-06-04

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CN201380042575.3A Expired - Fee Related CN104540965B (zh) 2012-06-18 2013-06-18 靶标检测和信号放大

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Country Link
US (1) US9725757B2 (OSRAM)
EP (1) EP2861770B1 (OSRAM)
JP (1) JP6336443B2 (OSRAM)
CN (1) CN104540965B (OSRAM)
AU (2) AU2013202354A1 (OSRAM)
CA (1) CA2876919C (OSRAM)
IL (1) IL236363A0 (OSRAM)
IN (1) IN2015DN00194A (OSRAM)
NZ (1) NZ629701A (OSRAM)
SG (1) SG11201408489PA (OSRAM)
WO (1) WO2013188912A1 (OSRAM)

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CN109055609B (zh) * 2018-08-08 2021-10-15 临沂大学 基于t4 dna聚合酶的西瓜花叶病毒检测传感器及其组装方法
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Also Published As

Publication number Publication date
US20160083785A1 (en) 2016-03-24
AU2013277929A1 (en) 2015-01-22
EP2861770B1 (en) 2018-01-31
JP6336443B2 (ja) 2018-06-06
SG11201408489PA (en) 2015-01-29
CN104540965A (zh) 2015-04-22
JP2015523864A (ja) 2015-08-20
WO2013188912A1 (en) 2013-12-27
IL236363A0 (en) 2015-02-26
AU2013202354A1 (en) 2014-01-16
EP2861770A1 (en) 2015-04-22
CA2876919A1 (en) 2013-12-27
CA2876919C (en) 2021-09-14
HK1205202A1 (en) 2015-12-11
NZ629701A (en) 2016-03-31
AU2013277929B2 (en) 2015-07-16
US9725757B2 (en) 2017-08-08
EP2861770A4 (en) 2015-12-23
IN2015DN00194A (OSRAM) 2015-06-12

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Granted publication date: 20190604