SG11201408489PA - Target detection and signal amplification - Google Patents

Target detection and signal amplification

Info

Publication number
SG11201408489PA
SG11201408489PA SG11201408489PA SG11201408489PA SG11201408489PA SG 11201408489P A SG11201408489P A SG 11201408489PA SG 11201408489P A SG11201408489P A SG 11201408489PA SG 11201408489P A SG11201408489P A SG 11201408489PA SG 11201408489P A SG11201408489P A SG 11201408489PA
Authority
SG
Singapore
Prior art keywords
international
nsw
methods
detection
sydney
Prior art date
Application number
SG11201408489PA
Inventor
Simon Mark Bone
Alison Velyian Todd
Timothy Daniel Meehan
Original Assignee
Speedx Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2012902551A external-priority patent/AU2012902551A0/en
Application filed by Speedx Pty Ltd filed Critical Speedx Pty Ltd
Publication of SG11201408489PA publication Critical patent/SG11201408489PA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9005—Enzymes with nucleic acid structure; e.g. ribozymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 27 December 2013 (27.12.2013) WIPOIPCT (10) International Publication Number WO 2013/188912 A1 (51) International Patent Classification: C12Q 1/68 (2006.01) CI20 1/44 (2006.01) C12N 9/22 (2006.01) (21) International Application Number: (22) International Filing Date: (25) Filing Language: (26) Publication Language: (30) Priority Data: 2012902551 2012903462 2013202354 PCT/AU2013/000651 18 June 2013 (18.06.2013) English English AU AU AU 18 June 2012 (18.06.2012) 10 August 2012 (10.08.2012) 3 April 2013 (03.04.2013) (71) Applicant: SPEEDX PTY LTD [AU/AU]; Suite G16, Na­ tional Innovation Centre, Australian Technology Park, 4 Cornwallis Street, Eveleigh, NSW 2015 (AU). (72) Inventors: BONE, Simon Mark; 2/56 Grosvenor Cres­ cent, Summer Hill, NSW 2130 (AU). TODD, Alison Ve- lyian; 10 Coneill Place, Glebe, NSW 2037 (AU). MEE- HAN, Timothy Daniel; 434 W 221 -229 Sydney Park Rd, Erskineville, NSW (AU). (74) Agent: SPRUSON & FERGUSON; GPO Box Sydney, New South Wales 2001 (AU). (81) Designated States (unless otherwise indicated, for every kind of national protection available)'. AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available)'. ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) CJ i-H o\ 00 00 i-H o CJ (54) Title: TARGET DETECTION AND SIGNAL AMPLIFICATION (57) Abstract: The present invention relates to compositions and methods for the detection of target molecules, and the amplifica­ tion of detectable signals generated by detection assays. More specifically, the present invention relates to methods utilizing catalytic nucleic acid enzymes to generate and/or amplify signal indicative a of the presence of target molecules (e.g. nucleic acids and pro - teins), and compositions for use in the methods.
SG11201408489PA 2012-06-18 2013-06-18 Target detection and signal amplification SG11201408489PA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AU2012902551A AU2012902551A0 (en) 2012-06-18 Target detection and signal amplification
AU2012903462A AU2012903462A0 (en) 2012-08-10 Target detection and signal amplification
AU2013202354A AU2013202354A1 (en) 2012-06-18 2013-04-03 Target detection and signal amplification
PCT/AU2013/000651 WO2013188912A1 (en) 2012-06-18 2013-06-18 Target detection and signal amplification

Publications (1)

Publication Number Publication Date
SG11201408489PA true SG11201408489PA (en) 2015-01-29

Family

ID=49767935

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201408489PA SG11201408489PA (en) 2012-06-18 2013-06-18 Target detection and signal amplification

Country Status (12)

Country Link
US (1) US9725757B2 (en)
EP (1) EP2861770B1 (en)
JP (1) JP6336443B2 (en)
CN (1) CN104540965B (en)
AU (2) AU2013202354A1 (en)
CA (1) CA2876919C (en)
HK (1) HK1205202A1 (en)
IL (1) IL236363A0 (en)
IN (1) IN2015DN00194A (en)
NZ (1) NZ629701A (en)
SG (1) SG11201408489PA (en)
WO (1) WO2013188912A1 (en)

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EP2753717B1 (en) 2011-09-09 2018-10-31 SpeeDx Pty Ltd Nucleic acid enzyme substrates
US9476090B2 (en) * 2013-05-21 2016-10-25 Stc.Unm Signal propagation biomolecules, devices and methods
RU2662969C2 (en) 2014-03-11 2018-07-31 Президент Энд Феллоуз Оф Гарвард Колледж High-throughput and highly multiplexed imaging with programmable nucleic acid probes
GB201415674D0 (en) * 2014-09-04 2014-10-22 Moorlodge Biotech Ventures Ltd Nucleic acid analysis
WO2016123419A1 (en) 2015-01-30 2016-08-04 President And Fellows Of Harvard College Microscope-free imaging
JP7085999B2 (en) * 2016-02-17 2022-06-17 プレジデント アンド フェローズ オブ ハーバード カレッジ Molecular programming tool
EP3516079B1 (en) 2016-09-20 2022-06-22 President and Fellows of Harvard College Molecular product authentication systems
US10934162B2 (en) * 2016-11-15 2021-03-02 The Governors Of The University Of Alberta MicroRNA initiated DNAzyme motor operating in living cells
EP3568492A4 (en) 2017-01-10 2021-03-17 President and Fellows of Harvard College Multiplexed signal amplification
AU2018252339A1 (en) * 2017-04-11 2019-07-11 Speedx Pty Ltd Detection cascades
CA3058202A1 (en) * 2017-04-17 2018-10-25 Montana State University Switch-like isothermal dna amplification demonstrating a non-linear amplification rate
CN107870242B (en) * 2017-10-12 2020-02-21 广东省生态环境技术研究所 Fluorescence detection kit based on aptamer
CN107663530B (en) * 2017-11-10 2020-07-03 中国海洋大学 Single-stranded nucleic acid detection kit, method and application thereof
EP3743525A4 (en) 2018-01-26 2021-09-29 President and Fellows of Harvard College Proximity detection methods and compositions
CN108642137B (en) * 2018-05-21 2021-11-30 福州大学 Method for detecting tumor biomarkers by using palindromic padlock probes
CN109055609B (en) * 2018-08-08 2021-10-15 临沂大学 Watermelon mosaic virus detection sensor based on T4 DNA polymerase and assembly method thereof
CN109097468A (en) * 2018-08-14 2018-12-28 南通市第人民医院 The detection kit and its application of OPCML gene promoter methylation level
CN109283147B (en) * 2018-09-20 2021-03-16 湖北师范大学 Homogeneous phase biosensing method for detecting kanamycin and application thereof
WO2020177059A1 (en) * 2019-03-04 2020-09-10 Nano And Advanced Materials Institute Limited System and method for detecting heavy metals and bacteria in an analyte
CN110607351B (en) * 2019-09-20 2022-11-01 济南大学 Chemiluminescence biosensor for detecting uracil glycosylase, and preparation method and application thereof
US20230221326A1 (en) * 2019-12-30 2023-07-13 Ultivue, Inc. Methods for Reducing Nonspecific Interactions on Biological Samples
CN112029827A (en) * 2020-09-04 2020-12-04 重庆工商大学 Design method of high-efficiency conversion rate DNA nano probe structure
WO2022061166A1 (en) * 2020-09-17 2022-03-24 Mammoth Biosciences, Inc. Compositions and methods for detection of a nucleic acid
CN113234800B (en) * 2021-05-13 2023-01-10 中山大学 Detection method of aflatoxin M1 and application thereof
WO2022271873A2 (en) * 2021-06-22 2022-12-29 Mammoth Biosciences, Inc. Programmable nuclease diagnostic device
US20230167487A2 (en) 2021-07-12 2023-06-01 Labsimply, Inc. Crispr cascade assay
WO2023114090A2 (en) * 2021-12-13 2023-06-22 Labsimply, Inc. Signal boost cascade assay
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
WO2023225651A2 (en) * 2022-05-19 2023-11-23 Logicink Corporation Pre-amplification-free target sequence detection with colormetric readout
US11982677B2 (en) 2022-10-02 2024-05-14 Vedabio, Inc. Dimerization screening assays
WO2024081156A1 (en) 2022-10-14 2024-04-18 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
CN116286806A (en) * 2023-03-21 2023-06-23 华中科技大学 Oligonucleotide with catalytic function, nucleic acid electronic device, logic gate complex and circuit
CN116732211B (en) * 2023-08-09 2023-10-27 湖南工程学院 Probe set and method for detecting mycobacterium bovis based on 8-17 deoxyribozyme and CRISPR-Cas13a trans-cleavage

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Also Published As

Publication number Publication date
CN104540965A (en) 2015-04-22
AU2013277929A1 (en) 2015-01-22
EP2861770A4 (en) 2015-12-23
JP2015523864A (en) 2015-08-20
EP2861770B1 (en) 2018-01-31
HK1205202A1 (en) 2015-12-11
IL236363A0 (en) 2015-02-26
CA2876919A1 (en) 2013-12-27
CA2876919C (en) 2021-09-14
NZ629701A (en) 2016-03-31
AU2013202354A1 (en) 2014-01-16
CN104540965B (en) 2019-06-04
US9725757B2 (en) 2017-08-08
AU2013277929B2 (en) 2015-07-16
EP2861770A1 (en) 2015-04-22
IN2015DN00194A (en) 2015-06-12
JP6336443B2 (en) 2018-06-06
US20160083785A1 (en) 2016-03-24
WO2013188912A1 (en) 2013-12-27

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