CA2876919C - Target detection and signal amplification - Google Patents

Target detection and signal amplification Download PDF

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Publication number
CA2876919C
CA2876919C CA2876919A CA2876919A CA2876919C CA 2876919 C CA2876919 C CA 2876919C CA 2876919 A CA2876919 A CA 2876919A CA 2876919 A CA2876919 A CA 2876919A CA 2876919 C CA2876919 C CA 2876919C
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Prior art keywords
substrate
enzyme
nucleic acid
catalytic
oligonucleotide
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CA2876919A
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English (en)
French (fr)
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CA2876919A1 (en
Inventor
Simon Mark BONE
Alison Velyian Todd
Timothy Daniel MEEHAN
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SpeeDx Pty Ltd
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SpeeDx Pty Ltd
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Priority claimed from AU2012902551A external-priority patent/AU2012902551A0/en
Application filed by SpeeDx Pty Ltd filed Critical SpeeDx Pty Ltd
Publication of CA2876919A1 publication Critical patent/CA2876919A1/en
Application granted granted Critical
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/9005Enzymes with nucleic acid structure; e.g. ribozymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2876919A 2012-06-18 2013-06-18 Target detection and signal amplification Active CA2876919C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
AU2012902551 2012-06-18
AU2012902551A AU2012902551A0 (en) 2012-06-18 Target detection and signal amplification
AU2012903462 2012-08-10
AU2012903462A AU2012903462A0 (en) 2012-08-10 Target detection and signal amplification
AU2013202354A AU2013202354A1 (en) 2012-06-18 2013-04-03 Target detection and signal amplification
AU2013202354 2013-04-03
PCT/AU2013/000651 WO2013188912A1 (en) 2012-06-18 2013-06-18 Target detection and signal amplification

Publications (2)

Publication Number Publication Date
CA2876919A1 CA2876919A1 (en) 2013-12-27
CA2876919C true CA2876919C (en) 2021-09-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2876919A Active CA2876919C (en) 2012-06-18 2013-06-18 Target detection and signal amplification

Country Status (11)

Country Link
US (1) US9725757B2 (OSRAM)
EP (1) EP2861770B1 (OSRAM)
JP (1) JP6336443B2 (OSRAM)
CN (1) CN104540965B (OSRAM)
AU (2) AU2013202354A1 (OSRAM)
CA (1) CA2876919C (OSRAM)
IL (1) IL236363A0 (OSRAM)
IN (1) IN2015DN00194A (OSRAM)
NZ (1) NZ629701A (OSRAM)
SG (1) SG11201408489PA (OSRAM)
WO (1) WO2013188912A1 (OSRAM)

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JP2023545038A (ja) * 2020-10-06 2023-10-26 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 中和標的のシグナルオン検出のための方法、組成物、キット、およびシステム
CN113234800B (zh) * 2021-05-13 2023-01-10 中山大学 一种黄曲霉毒素m1的检测方法及其应用
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Also Published As

Publication number Publication date
US20160083785A1 (en) 2016-03-24
AU2013277929A1 (en) 2015-01-22
EP2861770B1 (en) 2018-01-31
JP6336443B2 (ja) 2018-06-06
SG11201408489PA (en) 2015-01-29
CN104540965A (zh) 2015-04-22
JP2015523864A (ja) 2015-08-20
WO2013188912A1 (en) 2013-12-27
IL236363A0 (en) 2015-02-26
AU2013202354A1 (en) 2014-01-16
EP2861770A1 (en) 2015-04-22
CA2876919A1 (en) 2013-12-27
HK1205202A1 (en) 2015-12-11
NZ629701A (en) 2016-03-31
AU2013277929B2 (en) 2015-07-16
US9725757B2 (en) 2017-08-08
EP2861770A4 (en) 2015-12-23
CN104540965B (zh) 2019-06-04
IN2015DN00194A (OSRAM) 2015-06-12

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