CN104540594B - 使用高梯度磁场对粒子进行分类 - Google Patents

使用高梯度磁场对粒子进行分类 Download PDF

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CN104540594B
CN104540594B CN201380042623.9A CN201380042623A CN104540594B CN 104540594 B CN104540594 B CN 104540594B CN 201380042623 A CN201380042623 A CN 201380042623A CN 104540594 B CN104540594 B CN 104540594B
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conductive material
microfluidic
magnetic
magnetic conductive
channel
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CN104540594A (zh
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K.C.史密斯
R.哈古吉
T.A.巴伯
I.E.奥兹库穆尔
R.卡普
M.托纳
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General Hospital Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48735—Investigating suspensions of cells, e.g. measuring microbe concentration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/22—Details of magnetic or electrostatic separation characterised by the magnetic field, e.g. its shape or generation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
CN201380042623.9A 2012-06-25 2013-06-25 使用高梯度磁场对粒子进行分类 Active CN104540594B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261664051P 2012-06-25 2012-06-25
US61/664,051 2012-06-25
PCT/US2013/047710 WO2014004577A1 (en) 2012-06-25 2013-06-25 Sorting particles using high gradient magnetic fields

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CN104540594A CN104540594A (zh) 2015-04-22
CN104540594B true CN104540594B (zh) 2019-07-02

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US (3) US9278353B2 (enExample)
EP (1) EP2864051B1 (enExample)
JP (1) JP6382188B2 (enExample)
CN (1) CN104540594B (enExample)
IN (1) IN2014DN11074A (enExample)
WO (1) WO2014004577A1 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014004577A1 (en) 2012-06-25 2014-01-03 The General Hospital Corporation Sorting particles using high gradient magnetic fields
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN110186835B (zh) 2013-03-15 2022-05-31 Gpb科学有限公司 颗粒的片上微流体处理
US20160299132A1 (en) 2013-03-15 2016-10-13 Ancera, Inc. Systems and methods for bead-based assays in ferrofluids
US20160296945A1 (en) 2013-03-15 2016-10-13 Ancera, Inc. Systems and methods for active particle separation
CN105247042B (zh) 2013-03-15 2021-06-11 普林斯顿大学理事会 用于高通量纯化的方法和设备
US10202577B2 (en) 2013-10-18 2019-02-12 The General Hospital Corporation Microfluidic sorting using high gradient magnetic fields
IL247445B (en) 2014-02-26 2022-07-01 Brigham & Womens Hospital Inc System and method for cell levitation and monitoring
CA2966623C (en) 2014-11-03 2024-02-20 The General Hospital Corporation Concentrating particles in a microfluidic device
US10729879B2 (en) 2015-02-27 2020-08-04 Purdue Research Foundation Self-clearing catheters and methods of use thereof
US11285490B2 (en) 2015-06-26 2022-03-29 Ancera, Llc Background defocusing and clearing in ferrofluid-based capture assays
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
EP3341893A4 (en) * 2015-08-24 2019-03-06 GPB Scientific, LLC METHOD AND DEVICES FOR MULTI-STAGE CELL CLEANING AND CONCENTRATION
US10809180B2 (en) * 2015-09-28 2020-10-20 The Governing Council Of The University Of Toronto Device for magnetic profiling of particles in a flow
CA3000010C (en) 2015-10-02 2025-05-06 The Board Of Trustees Of The Leland Stanford Junior University Sorting of biological and non-biological fragments using magnetic levitation
JP6889169B2 (ja) * 2016-02-03 2021-06-18 アダプタス ソリューションズ プロプライエタリー リミテッド 磁界内の荷電粒子を制御するための装置及び方法
JP6738066B2 (ja) * 2016-08-08 2020-08-12 国立研究開発法人産業技術総合研究所 抽出フィルタおよびそれを備えたイムノクロマトグラフィー分析用キット
GB2552841B (en) * 2016-08-12 2020-05-20 Thermo Fisher Scient Bremen Gmbh Method of calibrating a mass spectrometer
JP6759881B2 (ja) * 2016-09-05 2020-09-23 アイシン精機株式会社 生体分子検出装置および生体分子検出装置の製造方法
CN107233941B (zh) * 2017-04-24 2019-11-26 深圳无微华斯生物科技有限公司 一种多重近红外荧光增强生物芯片筛选循环肿瘤细胞方法
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
GB201711804D0 (en) * 2017-07-21 2017-09-06 Mast Group Ltd Apparatus for conducting an assay
US11292000B2 (en) 2017-08-04 2022-04-05 University Of Georgia Research Foundation, Inc. Devices and methods for separating circulating tumor cells from biological samples
US11648559B2 (en) 2017-08-04 2023-05-16 University Of Georgia Research Foundation, Inc. Devices and methods for separating circulating tumor cells from biological samples
EP3669163A4 (en) * 2017-08-04 2021-08-11 University of Georgia Research Foundation, Inc. DEVICES AND METHODS FOR SEPARATING CIRCULATING TUMOR CELLS FROM BIOLOGICAL SAMPLES
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
JP7393328B2 (ja) 2017-09-01 2023-12-06 ジーピービー・サイエンティフィック・インコーポレイテッド マイクロ流体を使用した治療的に活性な細胞を調製する方法
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
CN107845477A (zh) * 2017-11-24 2018-03-27 西安交通大学 一种用于生物纳米磁珠粒径筛选与均化的可调磁场发生器
CN111757928A (zh) * 2017-12-01 2020-10-09 环球生命科技咨询美国有限责任公司 用于细胞富集和分离的方法
EP3723908A4 (en) * 2017-12-12 2021-07-14 Ancera, LLC MAGNETIC SCANNING SYSTEMS, METHODS AND DEVICES FOR FERROFLUID-BASED DOSAGE
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
US12564995B2 (en) 2019-01-31 2026-03-03 Illumina, Inc. Thermoformed, injection molded, and/or overmolded microfluidic structures and techniques for making the same
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
FR3100338B1 (fr) * 2019-08-30 2022-09-02 Centre Nat Rech Scient Kit et méthode de capture d’une molécule
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
KR102491075B1 (ko) * 2020-01-08 2023-01-27 주식회사 피엠랩 영구자석을 이용한 자기 분리 장치 및 이의 필터 구조체
WO2021183687A2 (en) 2020-03-10 2021-09-16 Cellares Corporation Systems, devices, and methods for cell processing
CN111450803B (zh) * 2020-03-16 2022-05-17 哈尔滨工业大学 一种吸附三苯甲烷类染料的磁性共价有机骨架化合物的制备方法及应用
CN111778159B (zh) * 2020-06-02 2023-01-31 东南大学 一种细胞用多级分选微流控芯片
WO2022061528A1 (zh) * 2020-09-22 2022-03-31 中国科学院深圳先进技术研究院 一种磁分选微流控芯片及其制作方法
EP3978119A1 (en) * 2020-09-30 2022-04-06 Levitas, Inc. Particle separator system, materials, and methods of use
FR3116215B1 (fr) * 2020-11-17 2024-03-29 Magia Diagnostics Cartouche comportant une pluralite de chambres d’analyse pour recevoir un liquide biologique
US20240094106A1 (en) * 2021-02-03 2024-03-21 University Of Georgia Research Foundation, Inc. Devices, kits, and methods for label-free inertial ferrohydrodynamic cell separation with high throughput and resolution
CN113109107B (zh) * 2021-03-24 2022-06-07 西安交通大学 一种气溶胶颗粒的富集装置
US20250161942A1 (en) * 2022-01-21 2025-05-22 Trustees Of Dartmouth College Microtransfer patterning of magnetic materials for microfluidic applications
EP4402192B1 (en) 2022-08-18 2025-07-02 10X Genomics, Inc. Droplet forming devices and methods having flourous diol additives
CN116251770B (zh) * 2023-03-15 2024-05-24 鸿翔环境科技股份有限公司 建筑垃圾磁流体分选设备及方法
US12399193B2 (en) 2023-05-05 2025-08-26 Cellares Corporation Systems, devices, and methods for combined cell processes
WO2025041064A2 (en) * 2023-08-21 2025-02-27 Cellares Corporation Systems, devices, and methods for automatic cell sorting
US12497587B2 (en) 2023-08-21 2025-12-16 Cellares Corporation Bioreactors and methods of their use in automatic cell processing systems

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU810277A1 (ru) * 1979-04-06 1981-03-07 Предприятие П/Я В-2126 Намагничивающий аппарат
CN101019026A (zh) * 2004-08-23 2007-08-15 基斯特-欧洲研究协会 应用免疫磁性分离法分离生物粒子的微观流体系统
RU68497U1 (ru) * 2006-12-06 2007-11-27 Владимир Евгеньевич Ляховецкий Устройство для магнитной обработки потока органической или неорганической жидкости или газа
KR20100040195A (ko) * 2008-10-09 2010-04-19 충북대학교 산학협력단 압전펌프
CN101738357A (zh) * 2008-11-19 2010-06-16 索尼株式会社 微粒分析装置、用于微粒分析的微流体芯片和微粒分析方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972721A (en) * 1996-03-14 1999-10-26 The United States Of America As Represented By The Secretary Of The Air Force Immunomagnetic assay system for clinical diagnosis and other purposes
US20050287583A1 (en) * 1997-01-21 2005-12-29 Promega Corporation Methods and kits for isolating biological target materials using silica magnetic particles
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5968820A (en) 1997-02-26 1999-10-19 The Cleveland Clinic Foundation Method for magnetically separating cells into fractionated flow streams
US6540896B1 (en) 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
CN1181337C (zh) * 2000-08-08 2004-12-22 清华大学 微流体系统中实体分子的操纵方法及相关试剂盒
TW496775B (en) * 1999-03-15 2002-08-01 Aviva Bioscience Corp Individually addressable micro-electromagnetic unit array chips
US6858439B1 (en) * 1999-03-15 2005-02-22 Aviva Biosciences Compositions and methods for separation of moieties on chips
CN1185492C (zh) * 1999-03-15 2005-01-19 清华大学 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用
PT1169062E (pt) 1999-04-09 2009-12-17 Amag Pharmaceuticals Inc Óxidos de ferro coloidais termoestáveis revestidos
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
DE60113287D1 (de) * 2000-06-14 2005-10-13 Univ Texas Systeme und verfahren zur zellteilbevölkerungsanalyse
AU2002320058A1 (en) 2001-06-06 2002-12-16 The General Hspital Corporation Magnetic-nanoparticle conjugates and methods of use
US7285412B2 (en) * 2001-07-27 2007-10-23 Surface Logix Inc. Device for magnetic immobilization of cells
US20030124194A1 (en) 2002-01-02 2003-07-03 Gaw Debra A. Amine functionalized superparamagnetic nanoparticles for the synthesis of bioconjugates and uses therefor
US20060041182A1 (en) * 2003-04-16 2006-02-23 Forbes Zachary G Magnetically-controllable delivery system for therapeutic agents
JP2006012529A (ja) * 2004-06-24 2006-01-12 Dainippon Ink & Chem Inc 燃料電池セパレータの製造装置及び燃料電池セパレータの製造方法
JP2006010529A (ja) * 2004-06-25 2006-01-12 Canon Inc 磁性粒子分離装置および分離方法
CA2606924C (en) 2005-05-09 2013-04-16 The General Hospital Corporation Water relaxation-based sensors
US20060292013A1 (en) * 2005-06-02 2006-12-28 Love Lonnie J Magnetocaloric pump for microfluidic applications
US9220831B2 (en) * 2005-10-06 2015-12-29 Children's Medical Center Corporation Device and method for combined microfluidic-micromagnetic separation of material in continuous flow
KR100846491B1 (ko) 2006-07-25 2008-07-17 삼성전자주식회사 미세유동 장치 내에서 표적 생체분자의 분리 및 정제를 위한 자성 비드 추출 장치
US7807454B2 (en) * 2006-10-18 2010-10-05 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for using the same
KR101503510B1 (ko) * 2007-02-09 2015-03-18 어드밴스드 리퀴드 로직, 아이엔씨. 자성 비즈를 이용하는 액적 작동기 장치 및 방법
EP2562531A3 (en) 2007-04-16 2013-03-06 The General Hospital Corporation d/b/a Massachusetts General Hospital Systems and methods for particle focusing in microchannels
WO2008147530A1 (en) * 2007-05-24 2008-12-04 The Regents Of The University Of California Integrated fluidics devices with magnetic sorting
EP2167216B1 (en) * 2007-06-29 2012-05-02 The President and Fellows of Harvard College Density-based methods for separation of materials, monitoring of solid supported reactions and measuring densities of small liquid volumes and solids
US7837379B2 (en) * 2007-08-13 2010-11-23 The Charles Stark Draper Laboratory, Inc. Devices for producing a continuously flowing concentration gradient in laminar flow
WO2009045551A1 (en) 2007-10-04 2009-04-09 The General Hospital Corporation Miniaturized magnetic resonance systems and methods
WO2009132151A2 (en) 2008-04-23 2009-10-29 The Regents Of The University Of California Microfluidic devices and methods of using same
KR101030637B1 (ko) 2009-02-02 2011-04-20 포항공과대학교 산학협력단 표면 결함 검사장치
US8417115B2 (en) 2009-07-09 2013-04-09 Finisar Corporation Quantifying link quality in an optoelectronic module
DE102009047801B4 (de) * 2009-09-30 2014-06-12 Siemens Aktiengesellschaft Durchflusskammer mit Zellleiteinrichtung
US20110123783A1 (en) * 2009-11-23 2011-05-26 David Sherrer Multilayer build processses and devices thereof
WO2011103143A1 (en) * 2010-02-16 2011-08-25 The University Of North Carolina At Chapel Hill Array of micromolded structures for sorting adherent cells
KR20120026959A (ko) * 2010-09-10 2012-03-20 인제대학교 산학협력단 자기 영동을 이용한 미세 입자 분리 장치 및 이를 이용하여 미세 입자를 분리하는 방법
WO2012054639A2 (en) * 2010-10-22 2012-04-26 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
KR101286939B1 (ko) 2011-04-21 2013-07-16 차희장 인조석 판재의 제조방법 및 상기 제조방법에 의해 생산된 인조석 판재를 이용한 시공방법
WO2014004577A1 (en) 2012-06-25 2014-01-03 The General Hospital Corporation Sorting particles using high gradient magnetic fields

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU810277A1 (ru) * 1979-04-06 1981-03-07 Предприятие П/Я В-2126 Намагничивающий аппарат
CN101019026A (zh) * 2004-08-23 2007-08-15 基斯特-欧洲研究协会 应用免疫磁性分离法分离生物粒子的微观流体系统
RU68497U1 (ru) * 2006-12-06 2007-11-27 Владимир Евгеньевич Ляховецкий Устройство для магнитной обработки потока органической или неорганической жидкости или газа
KR20100040195A (ko) * 2008-10-09 2010-04-19 충북대학교 산학협력단 압전펌프
CN101738357A (zh) * 2008-11-19 2010-06-16 索尼株式会社 微粒分析装置、用于微粒分析的微流体芯片和微粒分析方法

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