CN104488617A - Prevention method for plant bacterial canker - Google Patents
Prevention method for plant bacterial canker Download PDFInfo
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- CN104488617A CN104488617A CN201410759779.5A CN201410759779A CN104488617A CN 104488617 A CN104488617 A CN 104488617A CN 201410759779 A CN201410759779 A CN 201410759779A CN 104488617 A CN104488617 A CN 104488617A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/10—Sulfones; Sulfoxides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a prevention method for plant bacterial canker. The prevention method comprises the following steps of taking and dissolving veterinary-use florfenicol liquid under a water bath condition, adding veterinary-use gentamicin, 98% veterinary-use streptomycin sulphate and diethyl aminoethyl hexanoate, diluting with 15kg of water, and evenly stirring to obtain bactericide liquid; after a plant cultivated in a sunlight greenhouse suffers from the bacterial canker, thoroughly watering the plant before the bactericide liquid is sprayed, spraying the bactericide liquid in a sunny day, sealing the greenhouse after the bactericide liquid is sprayed, cleaning the greenhouse film of the greenhouse with dry cloth or a sponge for removing dust, improving the indoor temperature of the sunlight greenhouse to 53-55DEGC, and then, closing the greenhouse; repeatedly spraying the bactericide liquid, and closing the greenhouse one time; after water is added into the bactericide liquid to carry out dilution, carrying out root irrigation on the plant suffering from the bacterial canker. The prevention method has the advantages of scientific compatibility, good sterilization effect and obvious effect on preventing and curing diseases including plant bacterial canker, so that the plant is free from the infringement of bacteria in a whole growth process and does not generate drug resistance.
Description
Technical field
The present invention relates to a kind of prevention and controls of vegetative bacteria canker.
Background technology
Plant canker is that Michigan clavibacter infects caused bacterium class disease, and this germ invades primarily of the wound of plant, and seed and invalid body are survived the winter, and survival period can reach 2 years ~ 3 years, at high temperature, wets or susceptible disease in the not enough environment of illumination more.During outdoor cropping, when meeting the hot summer days, rainy or cloudy season, the morbidity of plant canker is comparatively serious.During Cultivation in Greenhouse, easily when early spring cultivates, morbidity is comparatively serious, and early spring, cultivation was generally June ~ July, and temperature and humidity is high, and canopy film is aging, when light transmittance goes down, provides favorable environment to the morbidity of vegetative bacteria canker.Germ morbidity temperature is limited to 1 DEG C ~ 33 DEG C, thermophilic 25 DEG C ~ 27 DEG C, and the plant upper blade that germ falls ill light occurs wilting phenomenon noon, sooner or later recovers normal, the fruits/plant easily aobvious disease that germ morbidity is heavy, finally causes total crop failure.
At present, the medicine for the treatment of plant canker generally adopts 14% Lip river ammonia copper water Ji, 77% can kill to obtain wetting powder, 500 times of 1:1:200 bordeaux mixture, the agricultural chemicals such as 72% agricultural streptomycin sulphate, 60% amber aliette wetting powder, bactericidal effect for treatment plant canker is undesirable, and easily makes germ develop immunity to drugs.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of compatibility science, and bactericidal effect is good, the prevention and controls of the vegetative bacteria canker do not developed immunity to drugs.
Technical solution of the present invention is:
A prevention and controls for vegetative bacteria canker, its concrete steps are as follows:
(1) getting concentration is 3.6g/10mL dirty solution type veterinary florfenicol liquid 20mL, dissolve under the water bath condition of 50 DEG C ~ 60 DEG C, add 10mL gentamicin for animals, 98% streptomycin sulphate for animals of 0.5g ~ 1g and the diethyl aminoethyl hexanoate of 0.8g ~ 1g, and dilute with 15kg water, stir, obtain sterilization liquid;
(2) plant of day-light greenhouse cultivation is after must going up canker, is watered by plant permeable before spray medicine, to select the morning 9 of fine day time ~ 11 time carry out spraying the sterilization liquid that step (1) prepares, every mu of spray value is 30 kg ~ 45kg; After spray medicine, booth is obturaged, carry out dedusting with the canopy film of dry cloth or sponge brush booth, to improve the light transmittance of canopy film; The indoor temperature of day-light greenhouse is brought up to 53 DEG C ~ 55 DEG C, after vexed canopy 10min ~ 15min, temperature is down to 15 DEG C ~ 32 DEG C;
(3) step (2) first time spray medicine complete after 1 day ~ 3 days, the plant continued going up canker according to step (2) sprays medicine and vexed canopy, after being cooled to 15 DEG C ~ 32 DEG C, the sterilization liquid that step (1) is prepared is added water after carrying out 1 times ~ 2 times dilutions, carry out filling root to the plant that must go up canker, every strain dosage is 200mL ~ 250mL.
The specification of described gentamicin for animals is 100,000 units/10mL.
Prescription of the present invention adopts the scientific basis of four kinds of different agents to be:
Florfenicol is the structural equivalents of chloramphenicol, the mechanism of action and antimicrobial spectrum similar with first alum mycin, can be combined with 50S subunit, suppress phthaloyl based transferase, thus suppress the extension of peptide chain, interferencing protein synthesizes.Florfenicol structurally instead of-the OH in chloramphenicol, first alum mycin on propane chain 3 carbon location with F atom; prevent bacterium transacetylase; acetylation on this position; therefore do not lived by enzyme affects; and that transacetylase and bacterial plasmid mediate is relevant to the drug resistance of chloramphenicol, first alum mycin; therefore Florfenicol can not produce the drug resistance of similar chloramphenicol mediation, and still responsive to many chloramphenicol resistance bacterial strains.
Gentamicin for animals is aminoglycosides universal antibiotic, and have the differential protein strong bonded in antibacterial and sterilization it and bacterium nucleoglucoprotein body subunit to multiple gram-negative, positive bacteria, interference nucleoglucoprotein body function, stops protein synthesis.
Streptomycin sulphate for animals has antibacterial action to tubercle bacillus and multiple Gram-positive bacillus.Poor to the effect of the most gram-positive cocci of Staphylococcus aureus.Streptococcus, pseudomonas aeruginosa and anaerobic bacteria are to this medicine resistance.After bacterium contacts this product, quantity produces drug resistance, namely reaches very high level in a short time.This product is antibacterial when low concentration, sterilization during higher concentration.
Diethyl aminoethyl hexanoate, is that one has high bioactive compound, is used on plant and promotes photosynthesis, improves drug effect, fertilizer efficiency, and separate and poison poisoning, improve crop quality, nontoxic pair of effect is a plant growth regulators.
Beneficial effect of the present invention:
Diethyl aminoethyl hexanoate and gentamicin for animals, streptomycin, Florfenicol compatibility have no adverse reaction, and have no side effect to agricultural product.Gentamicin for animals is by acting on the A subunit of DNA of bacteria helicase, suppress the synthesis of DNA and cause bacterial death after copying, it has the strong feature of antibacterial broad-spectrum action, especially has very strong bactericidal action to gram-negative bacteria, and applies on crops and also have same effect.To control vegetative bacteria canker class disease effect highly significant, on Pseudomonas solanacearum, soft rotten erwinia, control efficiency is also fine, as the bacterial disease control on solanaceous vegetables, bacterial wilt on melon, leaf vegetables or angular leaf spot control efficiency all very good, plant is not encroached on by germ in whole process of growth, and can not develop immunity to drugs.
Embodiment
embodiment 1
(1) 200mL dirty solution type veterinary florfenicol liquid (3.6g/10mL) is got, dissolve under the water bath condition of 50 DEG C, add 100mL gentamicin for animals (100,000 units/10mL), the 98wt% streptomycin sulphate for animals of 5g and the diethyl aminoethyl hexanoate of 10g, and dilute with 150kg water, stir, obtain sterilization liquid;
(2) tomato of day-light greenhouse cultivation is after must going up canker, is watered by plant permeable before spray medicine, to select the morning 9 of fine day time ~ 11 time carry out spraying the sterilization liquid that step (1) prepares, every mu of spray value is 30 kg; After spray medicine, booth is obturaged, carry out dedusting with the canopy film of dry cloth or sponge brush booth, to improve the light transmittance of canopy film; The indoor temperature of day-light greenhouse is brought up to 53 DEG C, and after vexed canopy 10min, temperature is down to 15 DEG C;
(3) step (2) first time spray medicine complete after 1 day, the tomato continued going up canker according to step (2) sprays medicine and vexed canopy, after being cooled to 15 DEG C, get the sterilization liquid 15kg that step (1) is prepared, add after 15kg water dilutes and carry out filling root to the tomato that must go up canker, every strain dosage is 200mL.
Tomato in Greenhouse canker serious illness adopts the method spray medicine of the embodiment of the present invention 1, vexed canopy, after once filling with root, the cure rate 99.8% of canker of tomato, recovered gradually by after the scab 3d that bacterium endangers, entirely heal after 5d, non-viable non-apoptotic cell obtains reparation, both canker of tomato can be prevented and treated, again Healthy culture is carried out to plant, three kinds of veterinary drugs in formula are organic chemistry compositions, after spraying into plant, to product i.e. safety but also environmental protection, even this formula is applied in pollution-free production base, product meets national standard, it is promising good recipe of curing the disease, be worthy to be popularized.
Embodiment 2
(1) 200mL dirty solution type veterinary florfenicol liquid (3.6g/10mL) is got, dissolve under the water bath condition of 60 DEG C, add 100mL gentamicin for animals (100,000 units/10mL), the 98wt% streptomycin sulphate for animals of 10g and the diethyl aminoethyl hexanoate of 8g, and dilute with 150kg water, stir, obtain sterilization liquid;
(2) plant of day-light greenhouse cultivation is after must going up canker, is watered by plant permeable before spray medicine, to select the morning 9 of fine day time ~ 11 time carry out spraying the sterilization liquid that step (1) prepares, every mu of spray value is 45kg; After spray medicine, booth is obturaged, carry out dedusting with the canopy film of dry cloth or sponge brush booth, to improve the light transmittance of canopy film; The indoor temperature of day-light greenhouse is brought up to 55 DEG C, and after vexed canopy 15min, temperature is down to 32 DEG C;
(3) step (2) first time spray medicine complete after 3 days, the plant continued going up canker according to step (2) sprays medicine and vexed canopy, after being cooled to 32 DEG C, get the sterilization liquid 15kg that step (1) is prepared, add after 30kg water dilutes and carry out filling root to the plant that must go up canker, every strain dosage is 250mL.
So-and-so 1 mu of Tomato in Greenhouse of palace, female Dou Yingzipai hedge village, Ka Zuo in July 2 county of 2007 there occurs canker, and the state of an illness has been emerging on fruit, and this 0.07 hectare tomato faces total crop failure soon.Adopt the method for the embodiment of the present invention 2 to treat, through twice spray medicine, vexed canopy, after once filling with root, find that the spot on sick fruit is healed entirely.
Embodiment 3
(1) 200mL dirty solution type veterinary florfenicol liquid (3.6g/10mL) is got, dissolve under the water bath condition of 55 DEG C, add 100mL gentamicin for animals (100,000 units/10mL), the 98wt% streptomycin sulphate for animals of 8g and the diethyl aminoethyl hexanoate of 9g, and dilute with 150kg water, stir, obtain sterilization liquid;
(2) plant of day-light greenhouse cultivation is after must going up canker, is watered by plant permeable before spray medicine, to select the morning 9 of fine day time ~ 11 time carry out spraying the sterilization liquid that step (1) prepares, every mu of spray value is 40kg; After spray medicine, booth is obturaged, carry out dedusting with the canopy film of dry cloth or sponge brush booth, to improve the light transmittance of canopy film; The indoor temperature of day-light greenhouse is brought up to 54 DEG C, and after vexed canopy 12min, temperature is down to 22 DEG C;
(3) step (2) first time spray medicine complete after 2 days, the plant continued going up canker according to step (2) sprays medicine and vexed canopy, after being cooled to 22 DEG C, get the sterilization liquid 15kg that step (1) is prepared, add after 22kg water dilutes and carry out filling root to the plant that must go up canker, every strain dosage is 220mL.
The point green pepper bacterial wilt application of 2009 Fang Shen villages of Kun Dou township of Nian Kazuo county, adopts the method for the embodiment of the present invention 3 to treat, through twice spray medicine, vexed canopy, after once filling with root, and cure rate 99.0%.
The canker of tomato application of 2010 publicly-operated sub-town, Nian Kazuo counties, adopts the method for the embodiment of the present invention 3 to treat, through twice spray medicine, vexed canopy, after once filling with root, and cure rate 99.2%.
The present invention since two thousand seven respectively in Anshan in Liaoning Province, area, territory, Panjin Deng15Ge county popularizes, every tomato there occurs the plot of Bacterial Canker Disease, as long as used the prevention and controls of vegetative bacteria canker, canker can be cured.Further, not only have special efficacy in treatment canker of tomato, and at blue or green bacterial wilt such as (peppery) green pepper, eggplant etc., portion of Sui is downright bad, and the diseases such as angular leaf spot also have therapeutic action.Since two thousand seven utilize this formula treatment tomato ulcer more than more than 20,000 mus, cure rate more than 99%.
Claims (2)
1. a prevention and controls for vegetative bacteria canker, is characterized in that: concrete steps are as follows:
(1) getting concentration is 3.6g/10mL dirty solution type veterinary florfenicol liquid 20mL, dissolve under the water bath condition of 50 DEG C ~ 60 DEG C, add 10mL gentamicin for animals, 98% streptomycin sulphate for animals of 0.5g ~ 1g and the diethyl aminoethyl hexanoate of 0.8g ~ 1g, and dilute with 15kg water, stir, obtain sterilization liquid;
(2) plant of day-light greenhouse cultivation is after must going up canker, is watered by plant permeable before spray medicine, to select the morning 9 of fine day time ~ 11 time carry out spraying the sterilization liquid that step (1) prepares, every mu of spray value is 30 kg ~ 45kg; After spray medicine, booth is obturaged, carry out dedusting with the canopy film of dry cloth or sponge brush booth; The indoor temperature of day-light greenhouse is brought up to 53 DEG C ~ 55 DEG C, after vexed canopy 10min ~ 15min, temperature is down to 15 DEG C ~ 32 DEG C;
(3) step (2) first time spray medicine complete after 1 day ~ 3 days, the plant continued going up canker according to step (2) sprays medicine and vexed canopy, after being cooled to 15 DEG C ~ 32 DEG C, the sterilization liquid that step (1) is prepared is added water after carrying out 1 times ~ 2 times dilutions, carry out filling root to the plant that must go up canker, every strain dosage is 200mL ~ 250mL.
2. the prevention and controls of vegetative bacteria canker according to claim 1, is characterized in that: the specification of described gentamicin for animals is 100,000 units/10mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410759779.5A CN104488617A (en) | 2014-12-12 | 2014-12-12 | Prevention method for plant bacterial canker |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410759779.5A CN104488617A (en) | 2014-12-12 | 2014-12-12 | Prevention method for plant bacterial canker |
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| CN104488617A true CN104488617A (en) | 2015-04-08 |
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| CN201410759779.5A Pending CN104488617A (en) | 2014-12-12 | 2014-12-12 | Prevention method for plant bacterial canker |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010432A (en) * | 2015-07-17 | 2015-11-04 | 广西南宁碧湾园林工程有限公司 | Camellia canker treatment drug and treatment method thereof |
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| CN102499253A (en) * | 2011-11-10 | 2012-06-20 | 中国农业大学 | Method for preparing composition of crosslinking nanometer silica and Schiff base copper as well as application |
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2014
- 2014-12-12 CN CN201410759779.5A patent/CN104488617A/en active Pending
Patent Citations (4)
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| CN101069516A (en) * | 2007-05-18 | 2007-11-14 | 山东省农业科学院植物保护研究所 | Plant disease preventing controlling agent |
| CN101069514A (en) * | 2007-06-19 | 2007-11-14 | 广东省昆虫研究所 | Sterilizing agent containing copper gluconate |
| CN101486982A (en) * | 2009-02-10 | 2009-07-22 | 西北农林科技大学 | Endogenetic streptomycete for preventing kiwi berry bacterial canker and preparation thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010432A (en) * | 2015-07-17 | 2015-11-04 | 广西南宁碧湾园林工程有限公司 | Camellia canker treatment drug and treatment method thereof |
| CN105010432B (en) * | 2015-07-17 | 2017-08-25 | 广西南宁碧湾园林工程有限公司 | The medicine and its treatment method of camellia canker |
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Application publication date: 20150408 |