KR101697258B1 - Composition for controlling plant disease comprising mixture of detoxificated sulfur powder as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a composition for preventing a plant disease, comprising, as an active component, a mixture prepared by mixing detoxicated sulfur powder, a Bacillus amyloliquefaciens culture medium, sodium hydroxide (NaOH), phosphorous acid (H_3PO_3), copper sulfate (CuSO_4), rock salt, and sodium silicate (Na_2SiO_3); and to a method for preventing a plant disease by spraying the composition for preventing a plant disease on crops.

Description

TECHNICAL FIELD The present invention relates to a composition for controlling plant diseases and a use thereof for use as an effective ingredient,

The present invention Admiral sulfur powder, Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens) culture solution, sodium hydroxide (NaOH), phosphorous acid (H ₃ PO _3), copper sulfate (CuSO _4), sodium chloride and sodium silicate (Na ₂ SiO ₃₎ The present invention relates to a composition for controlling plant diseases, which comprises a mixture as an active ingredient, and a method for controlling plant diseases by treating the plant disease control composition with a crop.

When growing crops, many plant pathogens, insects and weeds inhibit the growth of crops. If they are not controlled, crop yields will be reduced by 30 to 100%. So far, many synthetic pesticides have been developed and used to control these harmful organisms. However, due to misuse of plant diseases, insect pest and weed control, toxicity to human axis, groundwater pollution, environmental pollution, ecosystem disturbance, Various problems are caused. Accordingly, OECD member countries including Korea are implementing environment friendly agriculture policy to reduce the use of synthetic pesticides by 40%. As part of this policy, bio-pesticides are emerging as an alternative to synthetic pesticides.

To grow plants, various hormones must be synthesized, including gibberellin, cytokinin, and auxin. Among them, gibberellin affects the growth of the plant. When the gibberellin hormone is generated as a metabolite of the pathogen, the growth of the rice is accelerated.

Kidari disease was first reported in 1898 by Hori since it was known by Ito and Kimura in 1928 in Japan. Since Kidari disease is a seed-borne disease, it occurs in all countries where rice is cultivated and its occurrence varies depending on the region, variety, cultivation mode and seed sterilization. The scientific name of the pathogen is Gibberella fujikuroi ). This pathogen can be divided into complete and incomplete generations, which are usually caused by incomplete generations.

When rice seeds are planted in spring rice fields, infected seeds are sown, and the severely infected seeds will dry out when germinated, while moderately infected seeds will exhibit typical tsarism symptoms. Though they are infected with a weak infection, their pruning is somewhat small and their growth is somewhat limited. However, when the environment develops well during the growing season, a lot of spores grow out in the conduit, and a spore of cornstalk is formed on the stem surface. These spores become infected with seeds by infecting healthy rice yields again when they come out.

Ginseng is a crop belonging to the Ogakpyu family. It is cultivated for 3 ~ 5 years after transplanting seedling seeds grown for one year after seed sowing. In seedling cultivation after seed sowing, more than 1800 seeds per plant are grown in a single plant. Most of the ginseng mosaic disease is caused by Rhizoctonia solani , and the ginseng that has been affected by the mosaic disease has symptoms that the stems that come into contact with the ground dry up to dark brown. The medicines used for the control of ginseng mosaicism have been replaced by tolclofos-methyl from PCNB in the past and have been used for about 20 years. However, when applied to the residual standards of the foreign countries, There is a concern that the development of new control methods is necessary.

Korean Patent No. 1078163 discloses a rice seed-seed disease control agent, and Korean Patent No. 0330119 discloses a rice seed-disinfecting composition. However, a composition for controlling plant diseases comprising the mixture of a virgin sulfur powder of the present invention as an active ingredient .

BRIEF SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to provide a Gibberella fujikuroi ) or Rhizoctonia solani , which exhibit excellent antifungal activity against Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens) amyloliquefaciens culture medium, a mixture of sodium hydroxide (NaOH), phosphorous acid (H ₃ PO ₃ ), lactose (CuSO ₄ ), salt and sodium silicate (Na ₂ SiO ₃ ) . Accordingly, the present inventors have found that the above-mentioned mixture exhibits excellent controlling activity against rice gut disease or ginseng gall blight, and confirmed that it can be used as a natural fungicide for controlling plant diseases.

In order to solve the above problems, the present invention Admiral sulfur powder, Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens) culture solution, sodium hydroxide (NaOH), phosphorous acid (H ₃ PO _3), copper sulfate (CuSO _4), for a plant disease controlling composition containing a mixture of the sodium chloride and sodium silicate (Na ₂ SiO ₃₎ as an active ingredient to provide.

The present invention also provides a method for controlling a plant disease by treating the plant disease control composition with a seed of a crop or a crop.

According to the invention, Admiral sulfur powder, Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens) culture solution, sodium hydroxide (NaOH), phosphorous acid (H ₃ PO _3), copper sulfate (CuSO _4), sodium chloride and sodium silicate (Na ₂ SiO ₃ ) Is used as an active ingredient, the composition of the present invention is Gibberella < RTI ID = 0.0 > fujikuroi or Rhizoctonia solani , and thus can be useful for the development of environmentally friendly natural product fungicides and production of high value-added organic agricultural products.

Fig. 1 shows photographs of rice seeds immersed in a diluted solution mixed with sulfur mixed water of Production Example 1 and water, respectively.
Fig. 2 shows the results of cultivating rice with a rice seed dipped in a diluted solution mixed with the sulfur mixed water of Production Example 1 and water.
FIG. 3 shows the results obtained by inoculating Gibberella fujikuroi into a medium treated with PDA at a concentration of 1000 ppm of sulfuric water in Comparative Examples and Preparation Example 1, respectively.
FIG. 4 shows a photograph of dehydrated water after immersing ginseng seeds in sulfur mixed water.

According to an aspect of the invention there is provided Admiral sulfur powder, Bacillus amyl Lowry query Pacific Enschede (Bacillus amyloliquefaciens) culture solution, sodium hydroxide (NaOH), phosphorous acid (H ₃ PO _3), copper sulfate (CuSO _4), for a plant disease controlling composition containing a mixture of the sodium chloride and sodium silicate (Na ₂ SiO ₃₎ as an active ingredient to provide.

The plant disease control composition of the present invention can effectively control a plant fungal disease, and the plant fungal disease is preferably a plant disease caused by Gibberella spp. Or Rhizoctonia spp. May be, but is not limited to, rice gilt disease caused by Gibberella fujikuroi or ginseng gall bladder caused by Rhizoctonia solani .

In the composition for controlling plant diseases according to the present invention, the above-described virgin sulfur powder is preferably added to sulfur with Acidithiobacillus thiooxidans ), fermenting at 36 to 40 ° C. for 3 to 5 days, and drying at 70 to 90 ° C. for 2 to 4 days. More preferably, the fermentation can be carried out by adding acididobacillus thiooxidans Acidithiobacillus thiooxidans ) culture, followed by fermentation at 38 ° C for 4 days and drying at 80 ° C for 3 days. The thus prepared toxic sulfur powder effectively removes the toxicity contained in sulfur and does not affect plant growth even when treated with plant seeds.

In addition, in the plant disease controlling composition of the present invention, the mixture preferably contains 200-300 kg of a virgin sulfur powder, 700-900 L of Bacillus amyloliquefaciens culture medium, 180-240 NaOH (NaOH) kg of phosphorous acid, 6 to 8 kg of phosphorous acid (H ₃ PO ₃ ), 4 to 6 kg of copper sulfate (CuSO ₄ ), 13 to 17 kg of rock salt and 16 to 24 kg of sodium silicate (Na ₂ SiO ₃ ) More preferably 250 kg of virgin sulfur powder, 810 L of Bacillus amyloliquefaciens culture, 210 kg of sodium hydroxide (NaOH), 7 kg of phosphorous acid (H ₃ PO ₃ ), 5 kg of lactose (CuSO ₄ ) 15 kg of rock salt and 20 kg of sodium silicate (Na ₂ SiO ₃ ). The mixture prepared by mixing the above materials and the blending ratio has an advantage of excellent control effect of pathogenic bacteria causing plant diseases.

The composition for controlling a plant disease of the present invention may further comprise a substance contained in a commonly used insecticide or bactericide. In addition to the active ingredient, a pesticidally acceptable solid carrier, a liquid carrier, a liquid diluent, Gaseous diluent, solid diluent, or other suitable adjuvant such as an emulsifier, dispersant or foaming agent. The composition for controlling a mixture of the active ingredient and the above excipient may be formulated into various formulations known in the field of agrochemicals. For formulation, any of the formulation methods commonly used in the field of agrochemicals may be used.

The controlling composition of the present invention can be preferably formulated into a wettable powder, a granule, a powder, an oil, a spray, a film, a capsule or a gel, and a contact agent through a formulation such as a donut- As shown in Fig.

The composition for prevention of the present invention is a composition for controlling rice gut disease ( Gibberella fujikuroi ) or ginseng chalk ( Rhizoctonia solani , the mixture is preferably contained at a concentration of 0.002 to 0.01 liter per liter of water, but this can be appropriately adjusted in consideration of the degree of growth of the crop, the cultivation environment, and the degree of disease of the plant disease.

By treatment of the formulated controlling composition of the present invention as described above, the seed of the plant that require controlling rice tall disease (Gibberella fujikuroi ) or ginseng chalk ( Rhizoctonia solani ) can be controlled.

The present invention also provides a method for controlling a plant disease by treating the plant disease control composition with a seed of a crop or a crop. The crop may preferably be rice or ginseng, but is not limited thereto.

The plant disease may be a plant disease caused by Gibberella spp. Or Rhizoctonia spp., Preferably Gibberella spp. fujikuroi ) or ginseng gall bladder caused by Rhizoctonia solani , but the present invention is not limited thereto.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example  1: Sulfur Mixed water  Produce

1 g of sulfur was placed in an Erlenmeyer flask and 100 mL of medium was injected into sulfur sterilized three times at 100 ° C for 30 minutes. Inoculated with Acidithiobacillus thiooxidans culture medium, fermented at 38 ° C for 4 days And dried at 80 캜 for 3 days to prepare a virgin sulfur powder. The medium is ammonium sulfate in distilled water, _{1000 mL ((NH 4) 2} SO 4) 0.2 g, a first potassium phosphate _{(KH 2 PO 4) 3 g} , magnesium sulfate heptahydrate _{(MgSO 4 · 7H 2 O)} 0.5 g, sulfate 0.009 g of ferrous sulfate heptahydrate (FeSO ₄ .7H ₂ O) and 0.25 g of calcium chloride (CaCl ₂ ) were mixed, and the mixture was filtered and sterilized at 0.2 μm.

250 kg of the prepared virgin sulfur powder, 810 L of Bacillus amyloliquefaciens culture (2.6 × 10 ⁸ CFU / ml), 210 kg of sodium hydroxide (NaOH), 7 kg of phosphorous acid (H ₃ PO ₃ ) Sulfur mixed water was prepared by mixing 5 kg of lactose (CuSO ₄ ), 15 kg of rock salt and 20 kg of sodium silicate (Na ₂ SiO ₃ ).

Comparative Example  1: Sulfur Mixed water  Produce

Put 1 g of sulfur in the Erlenmeyer flask injected with 100 mL medium in a 100 eseo ℃ 30 minutes three times a sterile sulfur, useful microbial 13 species (Lactobacillus parapa than varnish (Lactobacillus parafarraginis), Lactobacillus para casei (Lactobacillus paracasei , Lactobacillus < RTI ID = 0.0 > tolerans , Lactobacillus buchneri , Lactobacillus < RTI ID = 0.0 > harbinensis , Lactobacillus perolens), Lactobacillus Caucasus ramno (Lactobacillus rhamnosus), Lactobacillus beksi North Hotel Syracuse (Lactobacillus vaccinostercus), acetonitrile bakteo you Roba N-Sys (Acetobacter lovaniensis ), Acetobacter peroxydans , Pichia fermentans ), Candida ethanolica ( Candida ethanolica and Saccharomycopsis schoenii were inoculated and fermented at 38 ° C for 4 days and dried at 80 ° C for 3 days to prepare sterile sulfur powder.

250 kg of the prepared sterile sulfur powder and 810 L of Lactobacillus parafarrhinis culture medium (2.6 x 10 ⁸ CFU / ml), caustic soda 200 kg, abalone shell 10 kg, tampon shell 5 kg, rock salt 15 kg , And 20 kg of sodium silicate were mixed to prepare a mixed water of sulfur.

Comparative Example  2: Sulfur Mixed water  Produce

A mixed water of sulfur was prepared in the same manner as in Preparation Example 1 except that 250 ml of the virgin sulfur powder, 810 L of Bacillus amyloliquefaciens culture (2.6 x 10 ⁸ CFU / ml) Sulfur mixed water was prepared by mixing 210 kg of sodium (NaOH), 7 kg of phosphorous acid (H ₃ PO ₃ ), 20 kg of rock salt and 20 kg of sodium silicate (Na ₂ SiO ₃ ).

Comparative Example  3: Sulfur Mixed water  Produce

A mixed water of sulfur was prepared in the same manner as in Preparation Example 1 except that 250 kg of virgin sulfur powder, 810 L of Bacillus amyloliquefaciens culture medium (2.6 × 10 ⁸ CFU / ml), 160 kg of sodium hydroxide (NaOH) 25 kg of phosphorous acid (H ₃ PO ₃ ), 10 kg of lactose (CuSO ₄ ), 25 kg of rock salt and 37 kg of sodium silicate (Na ₂ SiO ₃ ) were mixed to prepare a mixed water of sulfur.

Comparative Example  4: Sulfur Mixed water  Produce

A mixed water of sulfur was prepared in the same manner as in Preparation Example 1 except that 250 kg of virgin sulfur powder, 810 L of Bacillus amyloliquefaciens culture (2.6 × 10 ⁸ CFU / ml), 245 kg of sodium hydroxide (NaOH) , 3 kg of phosphorous acid (H ₃ PO ₃ ), 2 kg of copper sulfate (CuSO ₄ ), 2 kg of rock salt and 5 kg of sodium silicate (Na ₂ SiO ₃ ) were mixed.

Example  1: Disinfection of rice seed

40 kg of rice seed was immersed in a diluted solution prepared by mixing 200-1000 mL of the sulfur mixed water prepared in Preparation Example 1 with 100 L of water at 16 to 30 캜 for 1 to 2 days. In the non-treatment area, rice seed was immersed in water not diluted with sulfur mixed water (Fig. 1).

As a result of cultivating rice with pretreated rice seeds, rice seedling disease occurred in rice seedlings treated with untreated seedlings, but no rice seedlings disease occurred in rice seedlings pretreated with the sulfur mixed water of the present invention ).

Example  2: Chemical Substance Sulfur water Jibelera Fujikuroi  Evaluation of inhibitory activity

After the Gibberella fujikuroi was inoculated at the same concentration, hydrogen peroxide, formalin, and sulfur mixed water of Preparation Example 1 were treated by concentration to compare bacterial activities. As a result, the treatment of hydrogen peroxide and sulfuric water inhibited the growth of Jibelella fujicuroi from 30 ppm, and the treatment of formalin inhibited the growth of Jibelella fujicullo from 10 ppm. Therefore, the sulfur mixed water treatment of the present invention is expected to inhibit the growth of Jibelella fujicullo, thereby preventing the rice seedling disease.

Inhibitory activity of Gibberella fucicloide by concentration of chemical and sulfur water division
Treatment concentration (ppm) 0 10 30 50 Hydrogen peroxide - - + ++ formalin - + ++ ++ Sulfur mixed water - - + +

Example  3: Sulfur Mixed water By process Jibelera Fujikuroi  Control effect

The mixed water of Comparative Example and Production Example 1 was treated with a PDA (complete medium) at a concentration of 1000 ppm, respectively, and the culture was applied to a medium containing Gibberella fujikuroi ), the growth retarding effect was slightly observed even in the sulfur-mixed water treatment of Comparative Example 1. However, Comparative Examples 2 to 4 showed a higher control effect than Comparative Example 1, It was confirmed that the growth of Gibberella fujikuroi was completely inhibited in the sulfuric mixed water treatment of Preparation Example 1. [ Thus, the sulfuric acid water treatment of Production Example 1 yielded Gibberella < RTI ID = 0.0 > fujikuroi ) was inhibited and it was confirmed that it could be used as a product of prevention of rice seedling disease and an environmentally friendly disinfectant (Fig. 3).

Example  4: Ginseng Moss  Measure incidence

To 10 L of water, 4 kg of ginseng seeds were immersed at 16 to 30 ° C for 1 to 2 days and then dehydrated in a diluted solution prepared by mixing 20 to 100 mL of the mixed sulfur produced in Preparation Example 1 and Comparative Examples 1 to 4 . The control layer immersed the ginseng seed in water which did not dilute the sulfur mixed water (Fig. 4). The pretreated ginseng seeds were cultivated for 1 year after sowing, and the incidence of mildew disease was measured during 1 year seedling cultivation.

Measurement of the incidence of gingham mildew disease division Incidence (%) Non-treatment 6.6% Production Example 1 0.3% Comparative Example 1 3.8% Compare to 2 1.6% Comparative Example 3 0.9% Comparative Example 4 1.0%

As a result, the incidence was low in the sulfur-mixed water treatment of the preparation example 1 and the comparative example, compared with the non-treatment-free treatment. It was confirmed that the sulfur mixed water of Production Example 1 of the present invention is superior in the prevention effect of ginseng mosaic disease which can be occurred in the cultivation of ginseng in Production Example 1, as compared with other Comparative Examples.

Claims (8)

700 ~ 900 L of Bacillus amyloliquefaciens culture, 180 ~ 240 kg of sodium hydroxide (NaOH), phosphorous acid (H ₃ PO ₃ ) in the concentration of 200 ~ 300 kg of virgin sulfur powder, 2.6 × 10 ⁸ CFU / (Gibberella spp.) Containing as an active ingredient, a mixture of 6 to 8 kg of lactic acid, 4 to 6 kg of lactose (CuSO ₄ ), 13 to 17 kg of rock salt and 16 to 24 kg of sodium silicate (Na ₂ SiO ₃ ) For the control of ginseng recurring disease caused by rice bug disease or Rhizoctonia spp. delete delete 2. The method according to claim 1, wherein the virgin sulfur powder is prepared by inoculating a sulfuric acid culture with Acidithiobacillus thiooxidans , followed by fermentation and drying. A composition for inhibiting the growth of ginseng caused by a pathogenic bacterium or Rhizoctonia spp. delete A composition for inhibiting the growth of ginseng caused by rice bark disease or Rhizoctonia spp. Caused by Gibberella spp. According to any one of claims 1 to 4 to seeds of rice, ginseng or their seeds A method for controlling rice seedlings or ginseng gall bladder disease. delete delete

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