CN108651525A - A kind of granular pesticide containing bacillus subtilis and humic acid - Google Patents
A kind of granular pesticide containing bacillus subtilis and humic acid Download PDFInfo
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- CN108651525A CN108651525A CN201810778667.2A CN201810778667A CN108651525A CN 108651525 A CN108651525 A CN 108651525A CN 201810778667 A CN201810778667 A CN 201810778667A CN 108651525 A CN108651525 A CN 108651525A
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- Prior art keywords
- bacillus subtilis
- humic acid
- zhongshengmycin
- granular pesticide
- weight ratio
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of granular pesticide containing bacillus subtilis and humic acid, active constituent is made of Zhongshengmycin, bacillus subtilis and humic acid, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound, the weight ratio of Zhongshengmycin, bacillus subtilis and humic acid is 12：1:20‑50.The present invention is synergistic apparent for preventing crops bacterial wilt, advantageously reduces applications of pesticide amount, reduces pesticide cost.
Description
Technical field
The present invention relates to cultural control technical fields, are specifically related to a kind of pesticide containing bacillus subtilis and humic acid
Granula is mainly used for preventing the bacterial wilt of crops.
Background technology
Bacterial wilt (Ralstonia solanacearum) is a kind of important bacterial disease in agricultural production, from crop
Seedling can occur to the phase of yielding positive results, be Solanaceae, legume Major Diseases.Bacterial wilt is tomato, eggplant, capsicum, horse
The Major Diseases of the solanaceous vegetables such as bell potato, in high temperature and humidity, continuous cropping continuous cropping, geodepression soil is glutinous, field ponding, soil meta-acid, applies partially
When nitrogenous fertilizer, which is easy to happen.
The pathogen of bacterial wilt is just already present in soil before crop-planting, and can deposit for a long time in the soil
Living, general 2-3 still keeps pathogenicity.In agricultural production, peasant household, which usually not uses, is watered spray-on process prevention bacterial wilt
Custom, and onset peak period be watered spraying medication effect it is poor, cause disease that cannot be prevented in time and cause part children
The massive losses of seedling death.
Therefore it is badly in need of a kind of fungicide that can effectively prevent crops bacterial wilt, it can be in crop sowing time, transplanting time, ridging
Phase, disease early period of origination are applied in time, are the main paths for solving above-mentioned technical problem.
Invention content
It is an object of the present invention to be directed to the above technical problem, a kind of agriculture containing bacillus subtilis and humic acid is provided
Medicine granule is applied to prevention crops bacterial wilt.
The technical solution used in the present invention is：A kind of granular pesticide containing bacillus subtilis and humic acid, activity
Ingredient is made of Zhongshengmycin, bacillus subtilis and humic acid.
(1) Zhongshengmycin, English name：Zhongshengmycin is N- glycoside antibiotic, by inhibiting cause of disease thin
The peptide bond of mycoprotein matter generates, and eventually leads to bacterial death.It can not only inhibit the growth of hypha,hyphae, inhibit sprouting for spore
Hair plays the role of preventing fungal disease, can also stimulate the generation of the precursor substance of protective plant protecting agent and lignin in plant,
To improve the disease resistance of plant, Zhongshengmycin can resisting gram-positive, negative bacteria, mycobacteria, saccharomycete and silk
Shape fungi, especially to crops pathogenic bacteria such as dish Bacteria erwinia, avenae subsp.citrull, rice leaf spot bacteria, ring rot of apple
Germ, fusarium graminearum etc..
(2) bacillus subtilis, English name：Bacillus subtilis are a kind of microbial bactericides, by having
Cause of disease is killed and controlled to the mode of action for imitating the antibacterial material and site competition, induction of resistance of the life of ingredient producing bacillus subtilis
Bacterium, to achieve the purpose that controlling disease.
(3) humic acid is a kind of natural organic high molecular compound, complicated, has ion exchange, chelating, wadding
The functions such as solidifying and colloidal sol agriculturally have the function of improveing soil, stimulating growth, are enhancing degeneration-resistant, improving quality.
The granular pesticide, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% original
The weight ratio of medicine, Zhongshengmycin, bacillus subtilis and humic acid is 1-2：1:20-50, it is preferred that Zhongshengmycin, withered grass bud
The weight ratio of spore bacillus and humic acid is 2:1:30 or 1:1:30.
The weight percent of the granular pesticide, Zhongshengmycin is 0.05%-0.2%, the weight percent of humic acid
Than for 2.0%-5.0%, the weight percent of bacillus subtilis is 0.05-0.1%, i.e., bacillus subtilis is in pesticide granules
Content in agent be 500,000,000 spores/gram~1,000,000,000 spores/gram.
The granular pesticide further includes in addition to the active ingredient (s adjuvant, and adjuvant is filler, dispersant, wetting
Agent, binder, stabilizer or it is other be beneficial to active ingredient in the formulation stablize and drug effect play substance, be all pesticidal preparations
In commonly use or allow the various composition used.
The present invention has the following advantages and significant technique effect：
(1) the active constituent mechanism of action of the invention is different, and mutual mixture is synergistic apparent, can greatly improve medicine
Effect reduces Pesticide use amount and drug cost；Be conducive to delay existing germ to the potential resistance risk of single medicament, extend medicine
The service life of agent.
(2) present invention is prepared into granule, can be timely in crop sowing time, transplanting time, ridging phase, disease early period of origination
Using, without being watered, time and labour saving.
Specific implementation mode
Technical scheme of the present invention and technique effect are described further below by way of specific embodiment, institute of the present invention
The percentage stated is weight percentage.
One, indoor joint toxicity measuring test
Embodiment 1：Zhongshengmycin, bacillus subtilis, humic acid and its mixture survey the indoor virulence of bacterial wilt of tomato
It is fixed
Reference《Pesticide bioactivity evaluates SOP》, biometric standard method NY/T1156.16-2008 and NY/T1156.6-
2006.Using turbidity method：Ralstonia solanacearum of tomato (the Pseudomonas being grown in NA medium slants that will be cultivated in advance
Solanacearum) strain is diluted to 1 × 10 with sterile water7The suspension of a spore/mL concentration, to the NB culture mediums of each drug containing
100 μ L bacterium solutions are inoculated in processing respectively, is placed at 28 DEG C and vibrates (120r/min) culture, often processing sets 4 repetitions.Start to cultivate
The preceding turbidity for measuring each processing respectively measures and records the turbidity of each processing when control treatment reaches exponential phase.
According to survey data, the growth inhibition ratio of bacterium is calculated by formula (1), is indicated with percentage (%), result of calculation
Retain 2 significant digits.
In formula：
P---- growth inhibition ratios；
A0----blank control turbidity value added；
A1----chemicals treatment turbidity value added.
The growth inhibition ratio of bacterium is converted into probit value (y), liquor strength (μ g/mL) is converted into logarithm (x), with most
Small square law acquires virulence regression equation (y=a+bx), and thus calculates the EC of each medicament50Value, synegism evaluation side
Method is commented with reference to biometric standard method NY/T1156.6-2006 according to the co-toxicity coefficient method (CTC) of (1960) Sun＆Johnson
The synergistic effect of valence pharmacy mix, i.e. CTC≤80 be antagonism, 80<CTC<120 be summation action, and CTC >=120 are synergy
Effect.
Test result is shown in Table 1.
The toxicity test statistical form of 1 Zhongshengmycin of table, bacillus subtilis, humic acid and its mixture to ralstonia solanacearum of tomato
Indoor joint toxicity measuring the result shows that, Zhongshengmycin, bacillus subtilis, humic acid weight ratio be 1-2：1:
When 20-50, co-toxicity coefficient (CTC) is all higher than 120, has apparent synergistic effect；When Zhongshengmycin, bacillus subtilis, corruption
The weight ratio for growing acid is 1:1:30、2:1:When 30, co-toxicity coefficient (CTC) coefficient is respectively 185.1,178.1, and synergy is more aobvious
It writes, there is good application value in field trials.
Two, embodiment is prepared
Embodiment 1：0.1% Zhongshengmycin, 1,000,000,000 CFU/ grams of 3.0% humic acid granule of bacillus subtilis
Formula：Weight ratio according to Zhongshengmycin, bacillus subtilis and humic acid is 1：1:30, middle raw bacterium is weighed according to quantity
Element 0.1%, bacillus subtilis 0.1%, humic acid 3.0%, sodium lignin sulfonate 3.5% (dispersant), dodecyl sulphate
Sodium 2.5% (wetting agent), polyvinyl alcohol 3.6% (binder), white carbon 5.0% (filler), diatomite (filler) complement to
100%.
Embodiment 2：0.2% Zhongshengmycin, 1,000,000,000 CFU/ grams of 3.0% humic acid granule of bacillus subtilis
Formula：Weight ratio according to Zhongshengmycin, bacillus subtilis and humic acid is 2:1:30, middle raw bacterium is weighed according to quantity
Element 0.2%, bacillus subtilis 0.1%, humic acid 3.0%, naphthalenesulfonate formaldehyde condensation compound 2.5% (dispersant), dodecane
Base sodium sulphate 2.5% (wetting agent), polyvinyl alcohol 5.0% (binder), white carbon 15% (filler), bentonite (filler) are supplied
To 100%.
Embodiment 3：0.1% Zhongshengmycin, 5.0 hundred million CFU/ grams of 2.5% humic acid granule of bacillus subtilis
Formula：Weight ratio according to Zhongshengmycin, bacillus subtilis and humic acid is 2：1:50, middle raw bacterium is weighed according to quantity
Element 0.1%, bacillus subtilis 0.05%, humic acid 2.5%, carboxymethyl cellulose 3.5% (dispersant), dodecyl sulphate
Sodium 2.0% (wetting agent), polyvinyl alcohol 3.5% (binder), kaolin (filler) complement to 100%.
Embodiment 4：0.2% Zhongshengmycin, 10.0 hundred million CFU/ grams of 2.0% humic acid granule of bacillus subtilis
Formula:Weight ratio according to Zhongshengmycin, bacillus subtilis and humic acid is 2：1:20.Middle raw bacterium is weighed according to quantity
Element 0.2%, bacillus subtilis 0.1%, humic acid 2.0%, carboxymethyl cellulose 3.2% (dispersant), detergent alkylate sulphur
Sour sodium 3.5% (wetting agent), polyvinyl alcohol 4.2% (binder), white carbon (filler) 10%, kaolin (filler) complement to
100%.
Prepare the preparation method of embodiment 1-4：Each component is sufficiently mixed by formula rate, is crushed, after wetting with water again
It stirs, then is granulated with screw extruder pelletizer, is sieved after dry to get containing Zhongshengmycin bacillus subtilis
The fungicide product of bacterium humic acid, dosage form are granule.
Three, field trial embodiment
Embodiment 1：The formulation application of 1 gained of embodiment will be prepared in the field medicine effect test of control of plant bacterial wilt
(1) experimental design：Using the preparation prepared obtained by embodiment 1 as test group 1#, amount of application is 3.5kg/ mus；Respectively
With 0.3% Zhongshengmycin granule, 1#, amount of application are 5.0kg/ mus as a control group；10000000000 CFU/ grams of bacillus subtilises
2#, amount of application are 5.0kg/ mus to granula as a control group；3#, amount of application are 6.5% humic acid granule as a control group
5.0kg/ mu；1 blank control group (CK) is set.
(2) application method：Select continuous cropping first crop bacterial wilt of tomato that serious plot occurs, by test group 1#, control group 1#,
Control group 2#, control group 3# correspondence medicament uniformly impose on sowing cave, the tomato seedling cultivated is then transplanted into sowing cave,
Per 2 young plant of cave, 100 caves are each handled, in total 200 young plant.Keep soil moisture consistent in each processing, other farming operations are normal
It carries out.
(3) investigation method：7d after transplanting is determined and is survived strain number, and the blueness after statistics transplant survival to the fruit maturation later stage is withered
Sick hair rate, calculation formula are：
(4) test result is shown in Table 2.
| Test process | Investigate total strain number | Diseased plant number | Diseased plant rate (%) | Preventive effect (%) |
| Test group 1# | 195 | 6 | 3.08 | 92.27 |
| Control group 1# | 192 | 21 | 10.94 | 72.52 |
| Control group 2# | 198 | 26 | 13.13 | 67.00 |
| Control group 3# | 194 | 41 | 21.13 | 46.89 |
| Blank control (CK) | 196 | 78 | 39.80 | 0.00 |
From field medicine effect test, it can be seen that synergistic effect after Zhongshengmycin, bacillus subtilis and humic acid compounding
Obviously, the usage amount of active ingredient had not only been reduced, but also has shown the field efficacy better than single dose.
Claims (4)
1. a kind of granular pesticide containing bacillus subtilis and humic acid, which is characterized in that active constituent is by Zhongshengmycin, withered
Careless bacillus and humic acid composition, set content as 1,000,000,000,000 gemma/gram bacillus subtilis active compound be 100% active compound,
The weight ratio of Zhongshengmycin, bacillus subtilis and humic acid is 1-2：1:20-50.
2. granular pesticide according to claim 1, which is characterized in that Zhongshengmycin, bacillus subtilis and humic acid
Weight ratio be 2:1:30 or 1:1:30.
3. granular pesticide according to claim 1 or 2, which is characterized in that the weight percent of Zhongshengmycin is
The weight percent of 0.05%-0.2%, humic acid are 2.0%-5.0%, and the weight percent of bacillus subtilis is 0.05-
0.1%.
4. the granule according to claim 1-3 any one is in the application for preventing crops bacterial wilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810778667.2A CN108651525A (en) | 2018-07-16 | 2018-07-16 | A kind of granular pesticide containing bacillus subtilis and humic acid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810778667.2A CN108651525A (en) | 2018-07-16 | 2018-07-16 | A kind of granular pesticide containing bacillus subtilis and humic acid |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104086312A (en) * | 2014-07-16 | 2014-10-08 | 镇江贝思特有机活性肥料有限公司 | Organic/inorganic special pesticide fertilizer for preventing and controlling tomato bacterial wilt |
| CN104152372A (en) * | 2014-06-05 | 2014-11-19 | 广东省农业科学院农业资源与环境研究所 | Biocontrol strain G68 for preventing and treating plant diseases, and preparation method and application of microbial inoculant thereof |
-
2018
- 2018-07-16 CN CN201810778667.2A patent/CN108651525A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152372A (en) * | 2014-06-05 | 2014-11-19 | 广东省农业科学院农业资源与环境研究所 | Biocontrol strain G68 for preventing and treating plant diseases, and preparation method and application of microbial inoculant thereof |
| CN104086312A (en) * | 2014-07-16 | 2014-10-08 | 镇江贝思特有机活性肥料有限公司 | Organic/inorganic special pesticide fertilizer for preventing and controlling tomato bacterial wilt |
Non-Patent Citations (2)
| Title |
|---|
| 李晶等: "生防枯草芽孢杆菌的研究进展", 《安徽农业科学》 * |
| 龙友华等: "3%中生菌素可湿性粉剂防治番茄青枯病的效果初探", 《中国蔬菜》 * |
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