CN104435052A - 一种金银花中绿原酸的提取工艺 - Google Patents

一种金银花中绿原酸的提取工艺 Download PDF

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CN104435052A
CN104435052A CN201310439267.6A CN201310439267A CN104435052A CN 104435052 A CN104435052 A CN 104435052A CN 201310439267 A CN201310439267 A CN 201310439267A CN 104435052 A CN104435052 A CN 104435052A
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chlorogenic acid
flos lonicerae
honeysuckles
extraction
present
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彭琼
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Qingdao Zhongren Zhiye Biological Technology Co Ltd
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Qingdao Zhongren Zhiye Biological Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/21—Esters, e.g. nitroglycerine, selenocyanates
    • A61K31/215—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids
    • A61K31/216—Esters, e.g. nitroglycerine, selenocyanates of carboxylic acids of acids having aromatic rings, e.g. benactizyne, clofibrate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A61K36/355—Lonicera (honeysuckle)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
    • C07C69/66—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety
    • C07C69/73—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety of unsaturated acids
    • C07C69/732—Esters of carboxylic acids having esterified carboxylic groups bound to acyclic carbon atoms and having any of the groups OH, O—metal, —CHO, keto, ether, acyloxy, groups, groups, or in the acid moiety of unsaturated acids of unsaturated hydroxy carboxylic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
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Abstract

一种金银花中绿原酸的提取工艺涉及绿原酸的提取工艺，更具体地说，是涉及金银花中绿原酸的提取工艺。本发明提供了一种耗时短、耗能小、提取率高的金银花中绿原酸的提取工艺。本发明采用如下技术方案，利用超声波法对金银花中的绿原酸进行提取，工艺步骤为：取粉碎后的金银花粉末于试管中，加入料液比为1∶10的浓度为60～80％的乙醇溶液浸泡24h，调节体系的pH值为2～6，超声提取3～4次。

Description

一种金银花中绿原酸的提取工艺
技术领域
本发明涉及一种绿原酸的提取工艺，更具体地说，是涉及金银花中绿原酸的提取工艺。
 
背景技术
金银花为忍冬科多年生半常绿缠绕植物忍冬的花蕾及初开的花，又名忍冬花、银花、双花等，具有抗菌和增强机体免疫力的功能。我国大部分地区均产，以山东产量最大，河南产的质量较佳。
金银花中含有木犀草素、绿原酸、异绿原酸等化合物，其主要活性成分绿原酸含量为5.3％～ 5.7％，最高可达11.14％。绿原酸具有利胆、抗菌消炎、抗病毒、止血、增加白血球、缩短血凝和出血时间以及兴奋中枢神经系统等多种药用功能。金银花中绿原酸的传统提取方法有水提法、乙醇回流法、水提醇沉法、酶解法，这些方法大都耗时长、耗能大、提取率低。
发明内容
本发明就是针对上述问题，提供了一种耗时短、耗能小、提取率高的金银花中绿原酸的提取工艺。
为了实现本发明的上述目的，本发明采用如下技术方案，本发明利用超声波法对金银花中的绿原酸进行提取，工艺步骤为：取粉碎后的金银花粉末于试管中，加入料液比为1∶10 的浓度为60～80％的乙醇溶液浸泡24h，调节体系的pH 值为2～6，超声提取2～4次。
本发明的有益效果：
与传统的提取方法相比，本发明提取时间短、条件温和、提取率高，详细情况可见表1。
表1 不同绿原酸提取方法的比较
方法 吸光度 浓度/μg·ml 1 绿原酸含量/％
传统水提 1.195 36.01 3.60
超声水提 1.414 42.96 5.37
60％乙醇回流 1.642 50.20 5.02
70％乙醇回流 1.787 54.80 5.48
80％乙醇回流 1.670 51.10 5.11
60％乙醇超声 2.261 69.84 8.73
70％乙醇超声 2.432 75.28 9.41
80％乙醇超声 2.291 70.80 8.85
下面结合实施例，具体说明本发明的制备方法。
4、具体实施方式
本发明利用超声波法对金银花中的绿原酸进行提取，工艺步骤为：取粉碎后的金银花粉末于试管中，加入料液比为1∶10 的浓度为60～80％的乙醇溶液浸泡24h，调节体系的pH值为2～6，超声提取3～4次。
本发明选择乙醇体积分数、体系pH 值、超声提取次数作为影响金银花中绿原酸的提取效果的影响因素，可见表2、表3。
表2 三因素水平表
表3 三因素对金银花中绿原酸的提取效果的影响
序号 A B C 样品质量/g 吸光度 绿原酸含量/％
1 1 1 1 1.0030 1.843 7.05
2 1 2 2 1.0139 2.189 8.27
3 1 3 3 1.0094 2.551 8.79
4 2 1 2 1.0532 2.229 8.23
5 2 2 3 1.0104 2.671 9.58
6 2 3 1 1.0458 2.230 7.74
7 3 1 3 1.0096 2.562 8.89
8 3 2 1 1.0975 2.307 7.12
9 3 3 2 1.0573 2.259 8.18
作为一种优选方案，本发明的工艺步骤为：加入料液比为1 ∶ 10 的浓度为70％的乙醇溶液浸泡金银花24h，调节体系pH 值为4，超声提取4 次，得到绿原酸。

Claims (2)

1.金银花中绿原酸的提取工艺，其特征在于，本发明采用如下技术方案，利用超声波法对金银花中的绿原酸进行提取，工艺步骤为：取粉碎后的金银花粉末于试管中，加入料液比为1∶10 的浓度为60～80％的乙醇溶液浸泡24h，调节体系的pH 值为2～6，超声提取3～4次。
2.根据权利要求1 所述的金银花中绿原酸的提取工艺，其特征在于，本发明的工艺步骤为：加入料液比为1∶10 的浓度为70％的乙醇溶液浸泡金银花24h，调节体系pH 值为4，超声提取4 次，得到绿原酸。
CN201310439267.6A 2013-09-25 2013-09-25 一种金银花中绿原酸的提取工艺 Pending CN104435052A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114671757A (zh) * 2021-09-18 2022-06-28 水羊化妆品制造有限公司 一种忍冬花绿原酸的提取方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114671757A (zh) * 2021-09-18 2022-06-28 水羊化妆品制造有限公司 一种忍冬花绿原酸的提取方法

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