CN104273175B - A kind of fluorine-containing acid amides and the bactericidal composition of waxy Bacillus - Google Patents
A kind of fluorine-containing acid amides and the bactericidal composition of waxy Bacillus Download PDFInfo
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- CN104273175B CN104273175B CN201410510935.4A CN201410510935A CN104273175B CN 104273175 B CN104273175 B CN 104273175B CN 201410510935 A CN201410510935 A CN 201410510935A CN 104273175 B CN104273175 B CN 104273175B
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- waxy bacillus
- flutolanil
- fluorine
- acid amides
- waxy
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Abstract
The present invention relates to the bactericidal composition of a kind of fluorine-containing acid amides and waxy Bacillus, flutolanil is 10: 1～1 with the weight ratio of waxy Bacillus: 10, preferably 5: 1～1: 6；The content that flutolanil and waxy Bacillus account for gross weight is 10%～80%, preferably 20%～35%.The present invention has significantly synergy, and drug effect can be greatly improved, and reduces Pesticide use amount and drug cost.Meanwhile, the composition of the present invention significantly reduces the potential resistance risk respectively to flutolanil and waxy Bacillus for the existing germ.
Description
Technical field
The present invention relates to a kind of pesticide synergistic composition, the Bactericidal composite of specifically fluorine-containing acid amides and waxy Bacillus
Thing.
Background technology
Waxy Bacillus, microorganism belonging to genus preparation, has low toxicity, low-residual, feature free from environmental pollution.Wax gemma bar
Bacterium can be improved in germ and adverse circumstance are endangered primosome by crop and produce the Scavenging activity of oxygen by internal SOD enzyme, regulated crop
Cell microhabitat, safeguards the normal physiological metabolism of cell and biochemical reaction, improves resistance, tachyauxesis, improves yield and product
Matter.Waxy Bacillus early has been reported that for the preventing and treating of rice sheath blight disease, as Shayang County, Hubei Province bureau of agriculture deliver " 12.5%
Jinggangmeisu waxy Bacillus prevents and treats the test of rice sheath blight disease " demonstrate well ridge wax bud bacterium rice sheath blight disease is prevented
Effect is notable, has obvious effect of increasing production.
Flutolanil, chemical name: 3'-isopropoxy-2-(trifluoromethyl) benzanilide, is Nihon Nihyaku Co., Ltd
The new benzoyl of exploitation replaces aniline fungicides, is the new medicament preventing and treating rice sheath blight disease, has EDD long, prevention effect
Good, that energy paddy rice improves setting percentage advantage.Current flutolanil has been used in the preventing and treating of rice sheath blight disease, such as in Ministry of Agriculture's registration
There is the preventing and treating for rice sheath blight disease for the 20% fluorine amine azoxystrobin water dispersible granule.
Flutolanil is different with the mechanism of action of waxy Bacillus, uses the active ingredient of the different mechanism of action to carry out
Compounding, it is to delay disease to develop immunity to drugs conventional method, and according to the effect in production application, judge that this compounding is
No have synergistic effect.The obvious formula of synergistic effect, can significantly improve preventive effect, is substantially reduced the usage amount of agricultural chemicals,
Also can expand fungicidal spectrum, mitigate the pollution to environment.At present, flutolanil and waxy Bacillus is compounding, is used for rice banded sclerotial blight
The report that sick preventing and treating is not also related to.
Content of the invention
The technical problem to be solved is, provides a kind of with flutolanil, waxy Bacillus as active ingredient
Bactericidal composition, be used for preventing and treating rice sheath blight disease, it is achieved Synergistic substantially, mu originally lower, preventive effect is higher, and can delay disease
Evil develops immunity to drugs.
The present invention is to be achieved through the following technical solutions:
A kind of fluorine-containing acid amides and the bactericidal composition of waxy Bacillus, flutolanil with the weight ratio of waxy Bacillus is
10: 1～1: 10, preferably 5: 1～1: 6.
Described fluorine-containing acid amides and the bactericidal composition of waxy Bacillus, wherein effective active component content accounts for gross weight
10%～80%, preferably 20%～35%.
The bactericidal composition of described fluorine-containing acid amides and waxy Bacillus is according to side known in those skilled in the art
Method, adds corresponding additive and solvent, filler, makes water dispersible granules conventional on agricultural chemicals, suspending agent, wettable powder.
The auxiliary agent that the bactericidal composition of the present invention uses includes dispersant, wetting agent, antifreeze, defoamer, disintegrant, breast
Agent, stabilizer or other be of value to the material that active ingredient is stable in the formulation and drug effect plays, be all in pesticidal preparations often
With or allow use various compositions.
The present invention has the following advantages and significant technique effect, and flutolanil and waxy Bacillus belong to the mechanism of action not
Two same series bactericidal agents, have therebetween significantly synergy, drug effect can be greatly improved, reduce Pesticide use amount and use
Medicine cost.Meanwhile, the composition of the present invention significantly reduces existing germ potential to flutolanil and waxy Bacillus respectively
Resistance risk.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme and technique effect are described further, institute of the present invention
The percentage stated all is weight percentage.
The embodiment of the present invention is the method using Toxicity Determination and field test to combine.
Implementation of an application example one: flutolanil is compounding with waxy Bacillus to rice sheath blight disease Toxicity Determination
Determine through prerun after each medicament effective inhibition concentration scope, by flutolanil, waxy Bacillus and mixture preparation thereof
Dilute 5 concentration by active constituent content gradient series, compare with sterilized water.With reference to " farm-chemical indoor determination test standard
Then bactericide " carry out, use growth rate method determination method, variable concentrations medicament is added in sterilizing culture dish, every ware 1mL, then
Add the PDA culture medium 10mL of sterilizing fusing, be sufficiently mixed and make flat board, then the bacterium colony spreading cultivation is broken into=bacterium the dish of 0.8cm,
Every 1, ware, often processes 4 times and repeats, be placed in 25 DEG C of insulating boxs cultivation, and every 24h measures colony diameter 1 time.Calculate Net growth rate,
Relative inhibition, obtains toxicity regression formula, calculates EC50.The computing formula of bacteriostasis rate is as follows:
Mycelial growth inhibition rate (%)=(comparison colony diameter processes colony diameter)/comparison colony diameter × 100
Co-toxicity coefficient method (CTC) with the abundant method of Sun Yun evaluates the synergistic effect of pharmacy mix, i.e. CTC≤80 are made for antagonism
With 80<CTC<120 is summation action, and CTC>=120 are synergistic effect.
Result of the test is as shown in table 1:
Table 1 flutolanil, waxy Bacillus and built agent thereof the toxicity test analysis of results table to rice sheath blight disease
| Reagent agent | Proportioning | Regression equation (y=a+bx) | Coefficient correlation | EC50(mg/L) | Co-toxicity coefficient |
| Flutolanil | / | Y=3.1031+1.9215x | 0.9884 | 9.71 | / |
| Waxy Bacillus | / | Y=3.1647+1.5864x | 0.9930 | 14.35 | / |
| Flutolanil: waxy Bacillus | 5:1 | Y=3.5119+1.7292x | 0.9959 | 7.25 | 141.5 |
| Flutolanil: waxy Bacillus | 4:1 | Y=3.2085+1.9512x | 0.9942 | 8.28 | 125.3 |
| Flutolanil: waxy Bacillus | 2:1 | Y=3.6224+1.6705x | 0.9994 | 6.68 | 163.0 |
| Flutolanil: waxy Bacillus | 1:1 | Y=3.4774+1.8187x | 0.9934 | 6.87 | 168.5 |
| Flutolanil: waxy Bacillus | 1:2 | Y=3.5545+1.5622x | 0.9978 | 8.42 | 147.0 |
| Flutolanil: waxy Bacillus | 1:4 | Y=3.7126+1.3790x | 0.9890 | 8.58 | 152.6 |
| Flutolanil: waxy Bacillus | 1:6 | Y=3.5447+1.5913x | 0.9966 | 8.21 | 163.6 |
| Flutolanil: waxy Bacillus | 1:14 | Y=3.3987+1.4887x | 0.9957 | 11.90 | 116.9 |
As shown in Table 1, flutolanil, the EC to rice sheath blight disease for the waxy Bacillus50Be respectively 9.71mg/L and
14.35mg/L, makees when flutolanil and waxy Bacillus mixture in 5:1～1:6 proportioning all show obvious Synergistic
With, and when the percentage by weight of flutolanil and waxy Bacillus is 1:14, co-toxicity coefficient is 116.9, is showed only as being added
Effect.
The bactericidal composition of the fluorine-containing acid amides of implementation of an application example two and waxy Bacillus prevents and treats the field medicine of rice sheath blight disease
Effect test
This EE is at Wuming County doube bridge Zhenping, Nanning Lu Cun, and spraying time is September 27 in 2013 for the first time
Day, with the female medicine of the former medicine of flutolanil and waxy Bacillus in the range of percentage by weight is 5:1～1:6 according to people in the art
Method well known to Yuan, adds corresponding additive and solvent, filler, makes the mixed agent of flutolanil waxy Bacillus as examination
Test medicament, using 20% flutolanil wettable powder (commercial), 5% waxy Bacillus aqua (commercial) as comparison medicament, respectively
Cell uses identical application method, and water consumption is 45 kgs/acre.The rice sheath blight disease state of an illness is investigated, in the state of an illness initial stage the before medicine
Dispenser, every 7 days, dispenser once, was total to dispenser 2 times again.Within after last dispenser 7 days, 14 days, investigate the rice sheath blight disease state of an illness respectively and refer to
Count and calculate preventive effect.
Experimental result is as shown in table 2:
The bactericidal composition of the fluorine-containing acid amides of table 2 and waxy Bacillus prevents and treats the field test of rice sheath blight disease
As can be seen from Table 2, flutolanil and waxy Bacillus mixture are used for the preventing and treating of rice sheath blight disease, the effect of built agent
Fruit is substantially better than the effect that single dose is used alone, and illustrates that flutolanil and waxy Bacillus mixture have obvious synergistic effect,
Have obvious prevention effect to rice sheath blight disease, in process of the test, all growths of the rice seedling of each cell are normal.
Claims (1)
1. the bactericidal composition of a fluorine-containing acid amides and waxy Bacillus, it is characterised in that flutolanil and waxy Bacillus
Weight than for 5:1,4:1,2:1,1:1,1:2,1:4 or 1:6；Described flutolanil and waxy Bacillus account for composition gross weight
The content of amount is 20%～35%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410510935.4A CN104273175B (en) | 2014-09-29 | 2014-09-29 | A kind of fluorine-containing acid amides and the bactericidal composition of waxy Bacillus |
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|---|---|---|---|
| CN201410510935.4A CN104273175B (en) | 2014-09-29 | 2014-09-29 | A kind of fluorine-containing acid amides and the bactericidal composition of waxy Bacillus |
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| Publication Number | Publication Date |
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| CN104273175A CN104273175A (en) | 2015-01-14 |
| CN104273175B true CN104273175B (en) | 2016-11-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20110007168A (en) * | 2008-04-07 | 2011-01-21 | 바이엘 크롭사이언스 엘피 | Stable aqueous spore-containing formulation |
| WO2014086853A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a fungicidal compound |
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Inventor after: Lu Ninghai Inventor after: Yang Rui Inventor after: Shi Hongli Inventor after: Wu Limin Inventor after: Lang Jianfeng Inventor after: Huo Yunfeng Inventor before: Xie Xuye |
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Effective date of registration: 20160909 Address after: 453003 East District, Hongqi District, Henan, Xinxiang Applicant after: Henan Institute of Science and Technology Address before: Hengxian Yun Biao Zhen Daliang Cun Wei Liang Village Nanning city 530306 the Guangxi Zhuang Autonomous Region 3 No. 30 Applicant before: Xie Xuye |
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