CN106172429A - A kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides - Google Patents
A kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides Download PDFInfo
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- CN106172429A CN106172429A CN201610522182.8A CN201610522182A CN106172429A CN 106172429 A CN106172429 A CN 106172429A CN 201610522182 A CN201610522182 A CN 201610522182A CN 106172429 A CN106172429 A CN 106172429A
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- Prior art keywords
- benzothiostrobin
- acid amide
- amide fungicides
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- amisulbrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
Abstract
The invention discloses a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, effective ingredient benzothiostrobin and acid amide fungicides and conventional auxiliary element, acid amide fungicides one in amisulbrom or fluopicolide, the weight ratio of benzothiostrobin and acid amide fungicides is 20:1～1:10.Great number of indoor and outdoor experiments show that after being compounded, the bactericidal composition of the present invention multiple diseases to the growth that harms the crops, especially to by the microbial disease of Oomycete cause of disease, there is significant synergistic function, decrease Pesticide use amount simultaneously, reduce pesticide residual quantity on crops, alleviate the pollution to environment, delayed the drug-fast generation of pathogen, added the Pesticide use life-span.
Description
Technical field
The present invention relates to pesticide sterilizing composite and application thereof, specifically a kind of with benzothiostrobin and amide-type
Antibacterial is the pesticide sterilizing composite of effective ingredient, is mainly used in the preventing and treating of disease on crops.
Background technology
Benzothiostrobin (benzothiostrobin), belongs to methoxy acrylic bactericide, and the mechanism of action is line grain
Body respiratory chain cytochrome b c1 complex inhibitor, has a good safety to beneficial organism and crop, low toxicity and to environment friend
Good.Benzothiostrobin may be used for the preventing and treating of multiple diseases, particularly powdery mildew of cucumber and cucumber downy mildew is shown excellence
Preventive effect.
Acid amide fungicides occupies considerable status in antibacterial market, the world, through frequently as preventing and treating the special of oomycetes
With medicament, there is significantly protection, treatment and eradicant action, be widely used in the preventing and treating of plant disease；Amisulbrom
And fluopicolide (fluopicolide) is acid amide fungicides (amisulbrom).
Amisulbrom (amisulbrom), triazolylsulfonamide series bactericidal agent, this medicament mainly disease microbial to ovum
Having good prevention effect, it can suppress fungus to breathe and spore germination, and model of action is unique, in the case of low consumption
Keep high preventive effect, and there is the anti-function that drenches with rain.
Fluopicolide (fluopicolide), is a kind of new benzamides series bactericidal agent, to downy mildew, epidemic disease, late blight
The common oomycetes diseases such as disease, damping off have outstanding preventive effect, to crop and Environmental security.This product has excellent system and passes
The property led and stronger thin layer penetration power, Main Morphology each to pathogen all has good inhibiting effect, it is possible to for young leaves, stem,
Tuber, young fruit provide and protect comprehensively and persistently.
Pesticide individually long-time uses continuously and easily produces resistance risk and consumption increases risk, so increase cost and
Carrying capacity of environment, and the compositions with notable synergistic function is filtered out by agriculture chemical compounding, Pesticide use can not only be reduced
Amount, the use cost that reduces, minimizing resistance risk, moreover it is possible to reduce potential environmental risk.
The mixture of benzothiostrobin with acid amide fungicides is combined and conducts in-depth research, in substantial amounts of test by the present inventor
On the basis of, find that benzothiostrobin and acid amide fungicides combines in certain amount ranges, prevent and treat crop downy mildew, epidemic disease,
The Oomycete cause of disease microbial disease performance synergistic functions such as late blight, can give the formulations of pesticide by Technology.
After further study, the present invention is completed.
Summary of the invention
It is desirable to provide it is a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, this bactericidal composition
There is significant synergistic function, can be used for multiple diseases on crops, especially by the microbial disease of Oomycete cause of disease
The preventing and treating of (such as downy mildew, epidemic disease, late blight etc.).
Technical scheme: for achieving the above object, the present invention provides a kind of and kills containing benzothiostrobin and amide-type
The bactericidal composition of microbial inoculum, effective ingredient is benzothiostrobin and acid amide fungicides, and acid amide fungicides is selected from amisulbrom
Or the weight ratio of the one in fluopicolide, benzothiostrobin and acid amide fungicides is 20:1～1:10, preferred weight ratio is 10:
1～1:4；The weight summation of benzothiostrobin and acid amide fungicides percentage by weight in the composition is 1%～85%.
In bactericidal composition of the present invention, in addition to the active ingredient (s, solvent, cosolvent, emulsifying agent, dispersant, moistening are also included
Agent, thickening agent, antifreezing agent, cyst material, pH adjusting agent, defoamer, disintegrating agent, stabilizer, penetrating agent, diffusant, preservative,
The pesticide such as synergist, poisoning palliative, binding agent, density adjuster and filler allow use to assist into acceptable
Point, it being all conventional in pesticidal preparations or the composition of permission use, be not particularly limited, concrete composition and consumption are according to recipe requirements
Determined by test.
Bactericidal composition of the present invention can be prepared as the multiple application forms being suitable in agricultural production using, reasonable dose
Type has microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wettable powder and ultra low volume liquids.
The production technology of the various application forms of described bactericidal composition all belongs to existing known technology, does not repeats them here.
Bactericidal composition of the present invention is used for preventing and treating the microbial diseases of Oomycete cause of disease such as crop downy mildew, epidemic disease, late blight
Evil.
Compared with prior art, it is an advantage of the current invention that:
1, benzothiostrobin and acid amide fungicides compound and have significant synergistic function, are used alone bright than single dose
Show and improve the prevention effect of disease on crops.
2, benzothiostrobin and two kinds of active component of acid amide fungicides compound, and decrease Pesticide use amount, reduce use
Cost, alleviates the pollution to environment.
3, benzothiostrobin and two kinds of active component of acid amide fungicides compound, and the mechanism of action is different, and action site increases
Add, delayed the drug-fast generation of pathogen, added the service life of pesticide.
Detailed description of the invention
In order to be more fully understood that the essence of the present invention, below in conjunction with embodiment, present disclosure is described further,
But can not be considered as limitation of the present invention, the following stated is only used for explaining the present invention, for without departing from present invention spirit and principle
The amendment made, replace or improve, belong to the scope of protection of present invention.
Embodiment one: 25% benzothiostrobin amisulbrom microcapsule suspending agent
Following material is all weight percentage: benzothiostrobin 20%, amisulbrom 5%, melamine formaldehyde resin
(cyst material) 8%, fatty acid methyl ester (solvent) 10%, polyol fatty acid ester (emulsifying agent) 5%, maleic-acrylic acid are common
Polymers sodium salt (dispersant) 3%, sodium hydroxide (pH adjusting agent) 2%, deionized water complements to 100%.By above-mentioned active component,
Cyst material, solvent mix, and make to be dissolved into homogeneous oil phase, in a shear condition, join oil phase containing emulsifying agent, dispersion
Agent, pH adjusting agent aqueous phase solution in, surplus deionized water is supplied, and bi-material reacts at oil-water interfaces, formed capsule
Wall, can be prepared by 25% benzothiostrobin amisulbrom microcapsule suspending agent.
This embodiment is used for preventing and treating the late blight of potato: 25% benzothiostrobin amisulbrom microcapsule suspending agent is by 150g
A.i./ha consumption carries out dispenser for the first time at the late blight of potato initial stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 95.19% to the preventive effect of the late blight of potato；20% benzothiostrobin suspending agent is pressed
200g a.i./ha consumption carries out dispenser for the first time at late blight of potato their early stage, carries out second time dispenser, separately set after 7d
Clear water is processed as blank, and after second time dispenser, 14d is 70.48% to the preventive effect of the late blight of potato；200 grams per liter Yin
Azoles flusulfamide suspending agent carries out dispenser for the first time by 60g a.i./ha consumption at late blight of potato their early stage, carries out after 7d
Dispenser for the second time, separately sets clear water and is processed as blank, and after second time dispenser, the preventive effect of the late blight of potato is by 14d
79.63%.Visible, obvious synergy after benzothiostrobin and amisulbrom binary built, the lasting period is long, to Rhizoma Solani tuber osi
The preventive effect of late blight is used alone significantly better than each single dose, meanwhile, does not finds that this binary built medicament is to horse in process of the test
Bell potato produces poisoning.
Embodiment two: 24% benzothiostrobin fluopicolide microcapsule suspending agent
Following material is all weight percentage: benzothiostrobin 16%, fluopicolide 8%, melamine formaldehyde resin (capsule
Wall material) 8%, fatty acid methyl ester (solvent) 10%, polyol fatty acid ester (emulsifying agent) 5%, maleic acid-acrylic acid copolymer
Sodium salt (dispersant) 3%, sodium hydroxide (pH adjusting agent) 2%, deionized water complements to 100%.By above-mentioned active component, cyst wall
Material, solvent mix, and make to be dissolved into homogeneous oil phase, in a shear condition, join oil phase containing emulsifying agent, dispersant, pH
In the aqueous phase solution of regulator, surplus deionized water is supplied, and bi-material reacts at oil-water interfaces, forms cyst wall, i.e.
24% benzothiostrobin fluopicolide microcapsule suspending agent can be prepared.
This embodiment is used for preventing and treating the late blight of potato: 24% benzothiostrobin fluopicolide microcapsule suspending agent is by 200g
A.i./ha consumption carries out dispenser for the first time at the late blight of potato initial stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 94.85% to the preventive effect of the late blight of potato；20% benzothiostrobin suspending agent is pressed
200g a.i./ha consumption carries out dispenser for the first time at late blight of potato their early stage, carries out second time dispenser, separately set after 7d
Clear water is processed as blank, and after second time dispenser, 14d is 73.91% to the preventive effect of the late blight of potato；200 grams per liter fluorine
Pyrrole bacterium amine suspending agent carries out for the first time dispenser by 100g a.i./ha consumption at late blight of potato their early stage, carries out the after 7d
Secondary dispenser, separately sets clear water and is processed as blank, and after second time dispenser, the preventive effect of the late blight of potato is by 14d
76.25%.Visible, obvious synergy after benzothiostrobin and fluopicolide binary built, the lasting period is long, late to Rhizoma Solani tuber osi
The preventive effect of epidemic disease is used alone significantly better than each single dose, meanwhile, does not finds that this binary built medicament is to Ma Ling in process of the test
Potato produces poisoning.
Embodiment three: 20% benzothiostrobin amisulbrom suspending agent
Following material is all weight percentage: benzothiostrobin 16%, amisulbrom 4%, methyl naphthalene sulfonic acid sodium formaldehyde contract
Compound (wetting agent) 2%, phenethyl phenol polyethenoxy base ether phosphate (dispersant) 5%, ethylene glycol (antifreezing agent) 3%, xanthan
Glue (thickening agent) 1.5%, polyhydroxy benzenes propyl formate (preservative) 0.2%, C10-20 saturated fat acid compounds (froth breaking
Agent) 0.2%, triethanolamine (pH adjusting agent) 0.2%, vegetable oil (synergist) 2%, deionized water supplies 100%.By above-mentioned work
Property composition, wetting agent, dispersant, antifreezing agent, thickening agent, preservative, defoamer, pH adjusting agent, synergist and deionized water warp
Sand milling in high speed shear dispersion, sand mill, the high dispersive formed in water-medium, stable suspension system, can be prepared by
20% benzothiostrobin amisulbrom suspending agent.
This embodiment is used for preventing and treating cucumber downy mildew: 20% benzothiostrobin amisulbrom suspending agent by 150g a.i./
Ha consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 97.85% to the preventive effect of cucumber downy mildew；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 74.69% to the preventive effect of cucumber downy mildew；200 grams per liter amisulbroms hang
Floating agent carries out dispenser for the first time by 60g a.i./ha consumption at cucumber downy mildew their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 81.32% to the preventive effect of cucumber downy mildew.Visible, benzene thiophene bacterium
Obvious synergy after ester and amisulbrom binary built, the lasting period is long, is significantly better than the preventive effect of cucumber downy mildew
Each single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Cucumidis sativi in process of the test.
Embodiment four: 30% benzothiostrobin fluopicolide suspending agent
Following material is all weight percentage: benzothiostrobin 20%, fluopicolide 10%, methyl naphthalene sulfonic acid sodium formaldehyde are condensed
Thing (wetting agent) 2%, phenethyl phenol polyethenoxy base ether phosphate (dispersant) 5%, ethylene glycol (antifreezing agent) 3%, xanthan gum
(thickening agent) 1.5%, polyhydroxy benzenes propyl formate (preservative) 0.2%, C10-20 saturated fat acid compounds (defoamer)
0.2%, triethanolamine (pH adjusting agent) 0.2%, vegetable oil (synergist) 2%, deionized water supplies 100%.By above-mentioned activity
Composition, wetting agent, dispersant, antifreezing agent, thickening agent, preservative, defoamer, pH adjusting agent, synergist and deionized water are through height
Speed shears sand milling in dispersion, sand mill, the high dispersive formed in water-medium, stable suspension system, can be prepared by 30%
Benzothiostrobin fluopicolide suspending agent.
This embodiment is used for preventing and treating cucumber downy mildew: 30% benzothiostrobin fluopicolide suspending agent is by 200g a.i./ha
Consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser, separately set clear water and be processed as blank after 7d
Comparison, after second time dispenser, 14d is 96.12% to the preventive effect of cucumber downy mildew；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 75.18% to the preventive effect of cucumber downy mildew；200 grams per liter fluopicolides suspend
Agent carries out dispenser for the first time by 100g a.i./ha consumption at cucumber downy mildew their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 80.94% to the preventive effect of cucumber downy mildew.Visible, benzene thiophene bacterium
Obvious synergy after ester and fluopicolide binary built, the lasting period is long, is significantly better than each to the preventive effect of cucumber downy mildew
Single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Cucumidis sativi in process of the test.
Embodiment five: 35% benzothiostrobin amisulbrom aqueous emulsion
Following material is all weight percentage: benzothiostrobin 30%, amisulbrom 5%, Methyl palmitoleinate (solvent)
15%, styrylphenol polyoxyethylene ether (emulsifying agent) 10%, fatty acid glyceride polyoxyethylene ether (dispersant) 5%, vinegar
Acid anhydride (stabilizer) 3%, glycerol (antifreezing agent) 2%, organosiloxane (defoamer) 0.2%, sodium benzoate (preservative)
0.2%, carbamide (density adjuster) 1.5%, xanthan gum (thickening agent) 0.5%, deionized water supplies 100%.By above-mentioned activity
Composition, solvent, emulsifying agent, dispersant and stabilizer are mixed to form oil phase, antifreezing agent, defoamer, preservative, density adjuster and
Thickening agent dissolves formation aqueous phase in deionized water, slowly oil phase is added in aqueous phase under cutter effect, and continues to shear
About 30 minutes, prepare 35% benzothiostrobin amisulbrom aqueous emulsion.
This embodiment is used for preventing and treating tomato late blight: 35% benzothiostrobin amisulbrom aqueous emulsion by 150g a.i./
Ha consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 94.82% to the preventive effect of tomato late blight；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 76.41% to the preventive effect of tomato late blight；200 grams per liter amisulbroms hang
Floating agent carries out dispenser for the first time by 60g a.i./ha consumption at tomato late blight their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 77.65% to the preventive effect of tomato late blight.Visible, benzene thiophene bacterium
Obvious synergy after ester and amisulbrom binary built, the lasting period is long, is significantly better than the preventive effect of tomato late blight
Each single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Lycopersici esculenti in process of the test.
Embodiment six: 35% benzothiostrobin fluopicolide aqueous emulsion
Following material is all weight percentage: benzothiostrobin 25%, fluopicolide 10%, Methyl palmitoleinate (solvent)
15%, styrylphenol polyoxyethylene ether (emulsifying agent) 10%, fatty acid glyceride polyoxyethylene ether (dispersant) 5%, vinegar
Acid anhydride (stabilizer) 3%, glycerol (antifreezing agent) 2%, organosiloxane (defoamer) 0.2%, sodium benzoate (preservative)
0.2%, carbamide (density adjuster) 1.5%, xanthan gum (thickening agent) 0.5%, deionized water supplies 100%.By above-mentioned activity
Composition, solvent, emulsifying agent, dispersant and stabilizer are mixed to form oil phase, antifreezing agent, defoamer, preservative, density adjuster and
Thickening agent dissolves formation aqueous phase in deionized water, slowly oil phase is added in aqueous phase under cutter effect, and continues to shear
About 30 minutes, prepare 35% benzothiostrobin fluopicolide aqueous emulsion.
This embodiment is used for preventing and treating tomato late blight: 35% benzothiostrobin fluopicolide aqueous emulsion is by 200g a.i./ha
Consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser, separately set clear water and be processed as blank after 7d
Comparison, after second time dispenser, 14d is 98.34% to the preventive effect of tomato late blight；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 74.04% to the preventive effect of tomato late blight；200 grams per liter fluopicolides suspend
Agent carries out dispenser for the first time by 100g a.i./ha consumption at tomato late blight their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 82.54% to the preventive effect of tomato late blight.Visible, benzene thiophene bacterium
Obvious synergy after ester and fluopicolide binary built, the lasting period is long, is significantly better than each to the preventive effect of tomato late blight
Single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Lycopersici esculenti in process of the test.
Embodiment seven: 40% benzothiostrobin amisulbrom microemulsion
Following material is all weight percentage: benzothiostrobin 35%, amisulbrom 5%, Methyl palmitoleinate (solvent)
15%, ethanol (cosolvent) 5%, triphen ethyl phenol polyoxyethylene groups ether phosphate (emulsifying agent) 10%, sodium citrate (are stablized
Agent) 3%, organosilicon (penetrating agent) 1%, propyl isome (synergist) 2%, triethanolamine (pH adjusting agent) 0.2%, deionized water mend
Foot 100%.Above-mentioned active component solvent and cosolvent are completely dissolved, then add emulsifying agent, stabilizer, penetrating agent,
Synergist and pH adjusting agent mix homogeneously, be eventually adding deionized water, after being sufficiently stirred for, can be prepared by 40% benzothiostrobin Yin
Azoles flusulfamide microemulsion.
This embodiment is used for preventing and treating downy mildew of garpe: 40% benzothiostrobin amisulbrom microemulsion by 150g a.i./
Ha consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 91.97% to the preventive effect of downy mildew of garpe；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 71.39% to the preventive effect of downy mildew of garpe；200 grams per liter amisulbroms hang
Floating agent carries out dispenser for the first time by 60g a.i./ha consumption at downy mildew of garpe their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 73.65% to the preventive effect of downy mildew of garpe.Visible, benzene thiophene bacterium
Obvious synergy after ester and amisulbrom binary built, the lasting period is long, is significantly better than the preventive effect of downy mildew of garpe
Each single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Vitis viniferae in process of the test.
Embodiment eight: 40% benzothiostrobin fluopicolide microemulsion
Following material is all weight percentage: benzothiostrobin 25%, fluopicolide 15%, Methyl palmitoleinate (solvent)
15%, ethanol (cosolvent) 5%, triphen ethyl phenol polyoxyethylene groups ether phosphate (emulsifying agent) 10%, sodium citrate (are stablized
Agent) 3%, organosilicon (penetrating agent) 1%, propyl isome (synergist) 2%, triethanolamine (pH adjusting agent) 0.2%, deionized water mend
Foot 100%.Above-mentioned active component solvent and cosolvent are completely dissolved, then add emulsifying agent, stabilizer, penetrating agent,
Synergist and pH adjusting agent mix homogeneously, be eventually adding deionized water, after being sufficiently stirred for, can be prepared by 40% benzothiostrobin fluorine
Pyrrole bacterium amine microemulsion.
This embodiment is used for preventing and treating downy mildew of garpe: 40% benzothiostrobin fluopicolide microemulsion is by 200g a.i./ha
Consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser, separately set clear water and be processed as blank after 7d
Comparison, after second time dispenser, 14d is 93.62% to the preventive effect of downy mildew of garpe；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser after 7d, separately sets clear water and processes
For blank, after second time dispenser, 14d is 75.54% to the preventive effect of downy mildew of garpe；200 grams per liter fluopicolides suspend
Agent carries out dispenser for the first time by 100g a.i./ha consumption at downy mildew of garpe their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 78.36% to the preventive effect of downy mildew of garpe.Visible, benzene thiophene bacterium
Obvious synergy after ester and fluopicolide binary built, the lasting period is long, is significantly better than each to the preventive effect of downy mildew of garpe
Single dose is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Vitis viniferae in process of the test.
Embodiment nine: 10% benzothiostrobin amisulbrom ultra low volume liquids
Following material is all weight percentage: benzothiostrobin 7%, amisulbrom 3%, trimethylbenzene (solvent) 20%, N,
N-dimethyl acetylamide (solvent) 10%, terminal hydroxy group polyoxyethylene poly-oxygen propylene aether (emulsifying agent) 5%, calcium dodecyl benzene sulfonate
(wetting agent) 5%, brassin lactones (poisoning palliative) 0.02%, isophorone (penetrating agent) 2%, triacetyl glycerine is supplied
To 100%.After above-mentioned active component stirring solvent being dissolved, add emulsifying agent, wetting agent, poisoning palliative and infiltration
Agent, is thoroughly mixed uniformly, is eventually adding triacetyl glycerine, after being sufficiently stirred for, can be prepared by 10% benzothiostrobin Yin
Azoles flusulfamide ultra low volume liquids.
This embodiment is used for preventing and treating Citrullus vulgaris epidemic disease: 10% benzothiostrobin amisulbrom ultra low volume liquids is by 150g
A.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 93.04% to the preventive effect of Citrullus vulgaris epidemic disease；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 74.91% to the preventive effect of Citrullus vulgaris epidemic disease；200 grams per liter amisulbrom suspending agents
Carry out dispenser for the first time by 60g a.i./ha consumption at Citrullus vulgaris epidemic disease their early stage, carry out second time dispenser after 7d, separately set clear water
Being processed as blank, after second time dispenser, 14d is 79.24% to the preventive effect of Citrullus vulgaris epidemic disease.Visible, benzothiostrobin and indazole
Obvious synergy after flusulfamide binary built, the lasting period is long, independent significantly better than each single dose to the preventive effect of Citrullus vulgaris epidemic disease
Use, meanwhile, in process of the test, do not find that this binary built medicament produces poisoning to Citrullus vulgaris.
Embodiment ten: 10% benzothiostrobin fluopicolide ultra low volume liquids
Following material is all weight percentage: benzothiostrobin 7%, fluopicolide 3%, trimethylbenzene (solvent) 20%, N, N-
Dimethyl acetylamide (solvent) 10%, terminal hydroxy group polyoxyethylene poly-oxygen propylene aether (emulsifying agent) 5%, calcium dodecyl benzene sulfonate
(wetting agent) 5%, brassin lactones (poisoning palliative) 0.02%, isophorone (penetrating agent) 2%, triacetyl glycerine is supplied
To 100%.After above-mentioned active component stirring solvent being dissolved, add emulsifying agent, wetting agent, poisoning palliative and infiltration
Agent, is thoroughly mixed uniformly, is eventually adding triacetyl glycerine, after being sufficiently stirred for, can be prepared by 10% benzothiostrobin fluorine
Pyrrole bacterium amine ultra low volume liquids.
This embodiment is used for preventing and treating Citrullus vulgaris epidemic disease: 10% benzothiostrobin fluopicolide ultra low volume liquids is by 200g
A.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 97.19% to the preventive effect of Citrullus vulgaris epidemic disease；200g pressed by 20% benzothiostrobin suspending agent
A.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 73.67% to the preventive effect of Citrullus vulgaris epidemic disease；200 grams per liter fluopicolide suspending agents are pressed
100g a.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water after 7d
Being processed as blank, after second time dispenser, 14d is 83.65% to the preventive effect of Citrullus vulgaris epidemic disease.Visible, benzothiostrobin and fluorine pyrrole
Obvious synergy after bacterium amine binary built, the lasting period is long, individually makes the preventive effect of Citrullus vulgaris epidemic disease significantly better than each single dose
With, meanwhile, in process of the test, do not find that this binary built medicament produces poisoning to Citrullus vulgaris.
Embodiment 11: 65% benzothiostrobin amisulbrom water dispersible granules
Following material is all weight percentage: benzothiostrobin 55%, amisulbrom 10%, sodium lauryl sulphate are (wet
Profit agent) 5%, sodium acrylate and propionic acid amide. copolymer (dispersant) 5%, poly-naphthalene formaldehyde sulfonate salt 5% (diffusant), bentonite
(disintegrating agent) 5%, white sugar (binding agent) 0.2%, anhydrous calcium chloride (stabilizer) 0.2%, organosiloxane (defoamer)
0.2%, mannitol (pH adjusting agent) 0.2%, kieselguhr (filler) supplies 100%.By above-mentioned active component, wetting agent, dispersion
Agent, diffusant, disintegrating agent, binding agent, stabilizer, defoamer, pH adjusting agent and filler mix homogeneously, use micro jet
Pulverize, through mediating, be subsequently adding in fluidized-bed granulation dryer and carry out pelletize, dry, screening, can be prepared by 65% benzene thiophene bacterium
Ester amisulbrom water dispersible granules.
This embodiment is used for capsicum epidemic disease: 65% benzothiostrobin amisulbrom water dispersible granules presses 150g a.i./ha
Consumption carries out for the first time dispenser at capsicum epidemic disease their early stage, carries out second time dispenser after 7d, separately set clear water be processed as blank right
According to, after second time dispenser, 14d is 95.01% to the preventive effect of capsicum epidemic disease；20% benzothiostrobin suspending agent press 200g a.i./
Ha consumption carries out dispenser for the first time at capsicum epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as blank after 7d
Comparison, after second time dispenser, 14d is 71.92% to the preventive effect of capsicum epidemic disease；60g pressed by 200 grams per liter amisulbrom suspending agents
A.i./ha consumption carries out dispenser for the first time at capsicum epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 81.76% to the preventive effect of capsicum epidemic disease.Visible, benzothiostrobin and amisulbrom
Obvious synergy after binary built, the lasting period is long, is used alone the preventive effect of capsicum epidemic disease significantly better than each single dose, with
Time, in process of the test, do not find that this binary built medicament produces poisoning to Fructus Capsici.
Embodiment 12: 65% benzothiostrobin fluopicolide water dispersible granules
Following material is all weight percentage: benzothiostrobin 55%, fluopicolide 10%, sodium lauryl sulphate are (moistening
Agent) 5%, sodium acrylate and propionic acid amide. copolymer (dispersant) 5%, poly-naphthalene formaldehyde sulfonate salt 5% (diffusant), bentonite
(disintegrating agent) 5%, white sugar (binding agent) 0.2%, anhydrous calcium chloride (stabilizer) 0.2%, organosiloxane (defoamer)
0.2%, mannitol (pH adjusting agent) 0.2%, kieselguhr (filler) supplies 100%.By above-mentioned active component, wetting agent, dispersion
Agent, diffusant, disintegrating agent, binding agent, stabilizer, defoamer, pH adjusting agent and filler mix homogeneously, use micro jet
Pulverize, through mediating, be subsequently adding in fluidized-bed granulation dryer and carry out pelletize, dry, screening, can be prepared by 65% benzene thiophene bacterium
Ester fluopicolide water dispersible granules.
This embodiment is used for capsicum epidemic disease: 65% benzothiostrobin fluopicolide water dispersible granules is pressed 200g a.i./ha and used
Measure and carry out dispenser for the first time at capsicum epidemic disease their early stage, carry out second time dispenser after 7d, separately set clear water and be processed as blank,
After second time dispenser, 14d is 96.67% to the preventive effect of capsicum epidemic disease；20% benzothiostrobin suspending agent is pressed 200g a.i./ha and is used
Measure and carry out dispenser for the first time at capsicum epidemic disease their early stage, carry out second time dispenser after 7d, separately set clear water and be processed as blank,
After second time dispenser, 14d is 70.85% to the preventive effect of capsicum epidemic disease；200 grams per liter fluopicolide suspending agents press 100g a.i./
Ha consumption carries out dispenser for the first time at capsicum epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as blank after 7d
Comparison, after second time dispenser, 14d is 80.81% to the preventive effect of capsicum epidemic disease.Visible, benzothiostrobin and fluopicolide binary are multiple
Joining rear obvious synergy, the lasting period is long, is used alone the preventive effect of capsicum epidemic disease significantly better than each single dose, meanwhile,
Process of the test not finding, this binary built medicament produces poisoning to Fructus Capsici.
Embodiment 13: 75% benzothiostrobin amisulbrom wettable powder
Following material is all weight percentage: benzothiostrobin 65%, amisulbrom 10%, polyoxyethylene tri-styryl benzene
Based phosphates (wetting agent) 5%, sodium acrylate (expand with propionic acid amide. copolymer (dispersant) 5%, poly-naphthalene formaldehyde sulfonate salt 5%
Powder), white carbon (filler) 5%, kieselguhr (filler) supplies 100%.By above-mentioned by active component, wetting agent, dispersant, expansion
Powder and filler mixing, again through comminution by gas stream, mix homogeneously after mechanical activation comminution, can be prepared by 75% benzothiostrobin indazole sulphur bacterium
Amine wettable powder.
This embodiment views and admires Flos Chrysanthemi downy mildew for preventing and treating: 75% benzothiostrobin amisulbrom wettable powder is pressed
150g a.i./ha consumption carries out for the first time dispenser viewing and admiring Flos Chrysanthemi downy mildew their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 92.69% to the preventive effect viewing and admiring Flos Chrysanthemi downy mildew；20% benzene
Thiophene bacterium ester suspending agent carries out for the first time dispenser by 200g a.i./ha consumption viewing and admiring Flos Chrysanthemi downy mildew their early stage, carries out after 7d
Dispenser for the second time, separately sets clear water and is processed as blank, and after second time dispenser, the preventive effect viewing and admiring Flos Chrysanthemi downy mildew is by 14d
76.21%；200 grams per liter amisulbrom suspending agents are carried out viewing and admiring Flos Chrysanthemi downy mildew their early stage by 60g a.i./ha consumption
Dispenser for the first time, carries out second time dispenser, separately sets clear water and be processed as blank after 7d, after second time dispenser, 14d is to viewing and admiring
The preventive effect of Flos Chrysanthemi downy mildew is 73.24%.Visible, after benzothiostrobin and amisulbrom binary built, synergistic function shows
Writing, the lasting period is long, is used alone, significantly better than each single dose, the preventive effect viewing and admiring Flos Chrysanthemi downy mildew, meanwhile, in process of the test not
Find that this binary built medicament produces poisoning to viewing and admiring Flos Chrysanthemi.
Embodiment 14: 75% benzothiostrobin fluopicolide wettable powder
Following material is all weight percentage: benzothiostrobin 45%, fluopicolide 30%, polyoxyethylene tri-styryl phenyl
Phosphate (wetting agent) 5%, sodium acrylate and propionic acid amide. copolymer (dispersant) 5%, poly-naphthalene formaldehyde sulfonate salt 5% (diffusion
Agent), white carbon (filler) 5%, kieselguhr (filler) supplies 100%.By above-mentioned by active component, wetting agent, dispersant, diffusion
Agent and filler mixing, again through comminution by gas stream, mix homogeneously after mechanical activation comminution, can be prepared by 75% benzothiostrobin fluopicolide can
WP.
This embodiment views and admires Flos Chrysanthemi downy mildew for preventing and treating: 75% benzothiostrobin fluopicolide wettable powder is by 200g
A.i./ha consumption carries out for the first time dispenser viewing and admiring Flos Chrysanthemi downy mildew their early stage, carries out second time dispenser, separately set clear water after 7d
Being processed as blank, after second time dispenser, 14d is 91.58% to the preventive effect viewing and admiring Flos Chrysanthemi downy mildew；20% benzothiostrobin
Suspending agent carries out for the first time dispenser by 200g a.i./ha consumption viewing and admiring Flos Chrysanthemi downy mildew their early stage, carries out second time after 7d
Dispenser, separately sets clear water and is processed as blank, and after second time dispenser, 14d is 77.45% to the preventive effect viewing and admiring Flos Chrysanthemi downy mildew；
200 grams per liter fluopicolide suspending agents are carried out executing for the first time viewing and admiring Flos Chrysanthemi downy mildew their early stage by 100g a.i./ha consumption
Medicine, carries out second time dispenser, separately sets clear water and be processed as blank after 7d, after second time dispenser, 14d is to viewing and admiring Flos Chrysanthemi downy mildew
Sick preventive effect is 79.73%.Visible, obvious synergy after benzothiostrobin and fluopicolide binary built, the lasting period is long,
The preventive effect viewing and admiring Flos Chrysanthemi downy mildew is used alone significantly better than each single dose, meanwhile, in process of the test, does not finds that this binary is multiple
Dose out powders produces poisoning to viewing and admiring Flos Chrysanthemi.
Embodiment 15: benzothiostrobin and the compounding Toxicity Determination to late blight of potato pathogenic bacterium of amisulbrom
Test
Test target: late blight of potato pathogenic bacterium phytophthora infestans (Phytophthora infestans)
Test uses mycelial growth rate method with reference to " farm-chemical indoor determination test rule NY/T1156.2-2006 ".
Take the medicinal liquid that 5mL prepares join equipped with the hot culture medium of 45mL (rye (Secale cereale L.) culture medium, 45-50 DEG C.Rye (Secale cereale L.) culture medium prescription: black
Wheat 60g, sucrose 20g, agar powder 12g, add water to 1000mL) conical flask in, after shaking up, pour into rapidly diameter 90mm glass training
Supporting ware, each culture dish is poured band medicine culture medium 10mL into, is compared with the sterile water solution containing appropriate acetone and Tween 80,
Each process 4 repetition, horizontal rest, make flat board after cooling.Every ware accesses the bacterium cake one of diameter 0.4cm.All operations
All carry out sterile working at superclean bench.It is placed on dark culturing in the constant incubator of 20 DEG C after process, takes out after 10d.Adopt
Measure and record the colony diameter of each process by decussation method, calculate suppression ratio.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
Table 1, benzothiostrobin and amisulbrom pair
The Toxicity Determination result of late blight of potato pathogenic bacterium phytophthora infestans
| Medicament title and proportioning (weight ratio) | EC50(mg/L) | ATI | TTI | CTC |
| Benzothiostrobin | 3.42 | 100.00 | - | - |
| Amisulbrom | 2.27 | 150.66 | - | - |
| Benzothiostrobin: amisulbrom (20:1) | 1.86 | 183.87 | 102.41 | 179.54 |
| Benzothiostrobin: amisulbrom (15:1) | 1.69 | 202.37 | 103.17 | 196.15 |
| Benzothiostrobin: amisulbrom (10:1) | 1.31 | 261.07 | 104.61 | 249.57 |
| Benzothiostrobin: amisulbrom (7:1) | 1.17 | 292.31 | 106.33 | 274.91 |
| Benzothiostrobin: amisulbrom (4:1) | 1.05 | 325.71 | 110.13 | 295.75 |
| Benzothiostrobin: amisulbrom (2:1) | 1.02 | 335.29 | 116.89 | 286.84 |
| Benzothiostrobin: amisulbrom (1:1) | 1.07 | 319.63 | 125.33 | 255.03 |
| Benzothiostrobin: amisulbrom (1:2) | 1.11 | 308.11 | 133.77 | 230.33 |
| Benzothiostrobin: amisulbrom (1:4) | 1.20 | 285.00 | 140.53 | 202.80 |
| Benzothiostrobin: amisulbrom (1:7) | 1.34 | 255.22 | 144.33 | 176.83 |
| Benzothiostrobin: amisulbrom (1:10) | 1.56 | 219.23 | 146.05 | 150.11 |
During as it can be seen from table 1 benzothiostrobin and amisulbrom are compounding in the range of 20:1～1:10 (by weight),
Co-toxicity coefficient (CTC) is all higher than 120, and late blight of potato pathogenic bacterium phytophthora infestans is all shown synergistic function；Benzene thiophene
Bacterium ester and amisulbrom are in the range of 10:1～1:4 (by weight), and synergistic function is the most notable, co-toxicity coefficient
(CTC) all more than 200；Benzothiostrobin and amisulbrom compound with 4:1 (by weight), and synergistic function is the most notable,
Co-toxicity coefficient (CTC) is 295.75.Visible, benzothiostrobin and amisulbrom compound has reasonability and feasibility.
Embodiment 16: benzothiostrobin and the compounding Toxicity Determination to late blight of potato pathogenic bacterium of fluopicolide are tried
Test
Test target: late blight of potato pathogenic bacterium phytophthora infestans (Phytophthora infestans)
Test uses mycelial growth rate method with reference to " farm-chemical indoor determination test rule NY/T1156.2-2006 ".
Take the medicinal liquid that 5mL prepares join equipped with the hot culture medium of 45mL (rye (Secale cereale L.) culture medium, 45-50 DEG C.Rye (Secale cereale L.) culture medium prescription: black
Wheat 60g, sucrose 20g, agar powder 12g, add water to 1000mL) conical flask in, after shaking up, pour into rapidly diameter 90mm glass training
Supporting ware, each culture dish is poured band medicine culture medium 10mL into, is compared with the sterile water solution containing appropriate acetone and Tween 80,
Each process 4 repetition, horizontal rest, make flat board after cooling.Every ware accesses the bacterium cake one of diameter 0.4cm.All operations
All carry out sterile working at superclean bench.It is placed on dark culturing in the constant incubator of 20 DEG C after process, takes out after 10d.Adopt
Measure and record the colony diameter of each process by decussation method, calculate suppression ratio.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
Table 2, benzothiostrobin and the fluopicolide Toxicity Determination result to late blight of potato pathogenic bacterium phytophthora infestans
| Medicament title and proportioning (weight ratio) | EC50(mg/L) | ATI | TTI | CTC |
| Benzothiostrobin | 3.95 | 100.00 | - | - |
| Fluopicolide | 2.79 | 141.58 | - | - |
| Benzothiostrobin: fluopicolide (20:1) | 2.31 | 171.00 | 101.98 | 167.68 |
| Benzothiostrobin: fluopicolide (15:1) | 2.04 | 193.63 | 102.60 | 188.72 |
| Benzothiostrobin: fluopicolide (10:1) | 1.76 | 224.43 | 103.78 | 216.26 |
| Benzothiostrobin: fluopicolide (7:1) | 1.54 | 256.49 | 105.20 | 243.81 |
| Benzothiostrobin: fluopicolide (4:1) | 1.39 | 284.17 | 108.32 | 262.34 |
| Benzothiostrobin: fluopicolide (2:1) | 1.21 | 326.45 | 113.86 | 286.71 |
| Benzothiostrobin: fluopicolide (1:1) | 1.18 | 334.75 | 120.79 | 277.13 |
| Benzothiostrobin: fluopicolide (1:2) | 1.27 | 311.02 | 127.72 | 243.52 |
| Benzothiostrobin: fluopicolide (1:4) | 1.42 | 278.17 | 133.26 | 208.74 |
| Benzothiostrobin: fluopicolide (1:7) | 1.69 | 233.73 | 136.38 | 171.38 |
| Benzothiostrobin: fluopicolide (1:10) | 1.98 | 199.49 | 137.80 | 144.77 |
During from table 2 it can be seen that benzothiostrobin and fluopicolide are compounding in the range of 20:1～1:10 (by weight), altogether
Poison coefficient (CTC) is all higher than 120, and late blight of potato pathogenic bacterium phytophthora infestans is all shown synergistic function；Benzene thiophene bacterium
Ester and fluopicolide are in the range of 10:1～1:4 (by weight), and synergistic function is the most notable, and co-toxicity coefficient (CTC) is equal
More than 200；Benzothiostrobin and fluopicolide compound with 2:1 (by weight), and synergistic function is the most notable, co-toxicity coefficient
(CTC) it is 286.71.Visible, benzothiostrobin and fluopicolide compound has reasonability and feasibility.
Embodiment 17: benzothiostrobin and the compounding Toxicity Determination to cucumber downy mildew pathogenic bacterium of amisulbrom are tried
Test
Test target: cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew (Pseudoperonospora cubensis)
This test uses pot-culture method.Choosing the two panels leaf period cucumber seedling that growing way is consistent, each process selects 5 basins for examination melon
Seedling, numbers standby.Spray under 50PSI pressure with Potter spray tower, every basin 5mL.Each medicament arranges 5 Concentraton gradient,
With spray equivalent clear water as blank.Spray inoculation bacterium of downy mildew of cucumber sporangia suspension, spore after chemicals treatment 24h
The configuration process of capsule suspension is as follows: take the cucumber leaves picking up from field with cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew,
Dip the Sporangium in the distillation washing lower back face of about 10 DEG C with brush pen, being made into concentration is 3 × 105The Sporangium of individual/mL suspends
Liquid.Cucumber seedling is placed in growth cabinet after inoculation (relative humidity 100%, temperature 15-20 DEG C) cultivate, after 24h, keeps temperature
Spending 15-24 DEG C, relative humidity about 90% moisturizing induction, 10d " Invest, Then Investigate " records incidence, calculates disease index and preventing and treating effect
Really.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
Table 3, benzothiostrobin and the amisulbrom Toxicity Determination to cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew
Result
| Medicament title and proportioning (weight ratio) | EC50(mg/L) | ATI | TTI | CTC |
| Benzothiostrobin | 3.76 | 100.00 | - | - |
| Amisulbrom | 2.48 | 151.61 | - | - |
| Benzothiostrobin: amisulbrom (20:1) | 2.04 | 184.31 | 102.46 | 179.88 |
| Benzothiostrobin: amisulbrom (15:1) | 1.85 | 203.24 | 103.23 | 196.88 |
| Benzothiostrobin: amisulbrom (10:1) | 1.59 | 236.48 | 104.69 | 225.89 |
| Benzothiostrobin: amisulbrom (7:1) | 1.37 | 274.45 | 106.45 | 257.82 |
| Benzothiostrobin: amisulbrom (4:1) | 1.14 | 329.82 | 110.32 | 298.97 |
| Benzothiostrobin: amisulbrom (2:1) | 1.17 | 321.37 | 117.20 | 274.21 |
| Benzothiostrobin: amisulbrom (1:1) | 1.21 | 310.74 | 125.81 | 246.99 |
| Benzothiostrobin: amisulbrom (1:2) | 1.25 | 300.80 | 134.41 | 223.79 |
| Benzothiostrobin: amisulbrom (1:4) | 1.32 | 284.85 | 141.29 | 201.61 |
| Benzothiostrobin: amisulbrom (1:7) | 1.46 | 257.53 | 145.16 | 177.41 |
| Benzothiostrobin: amisulbrom (1:10) | 1.67 | 225.15 | 146.92 | 153.25 |
During from table 3 it can be seen that benzothiostrobin and amisulbrom are compounding in the range of 20:1～1:10 (by weight),
Co-toxicity coefficient (CTC) is all higher than 120, and cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew is all shown synergistic function；Benzene
Thiophene bacterium ester and amisulbrom are in the range of 10:1～1:4 (by weight), and synergistic function is the most notable, co-toxicity coefficient
(CTC) all more than 200；Benzothiostrobin and amisulbrom compound with 4:1 (by weight), and synergistic function is the most notable,
Co-toxicity coefficient (CTC) is 298.97.Visible, benzothiostrobin and amisulbrom compound has reasonability and feasibility.
Embodiment 18: benzothiostrobin and the compounding Toxicity Determination to cucumber downy mildew pathogenic bacterium of fluopicolide are tested
Test target: cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew (Pseudoperonospora cubensis)
This test uses pot-culture method.Choosing the two panels leaf period cucumber seedling that growing way is consistent, each process selects 5 basins for examination melon
Seedling, numbers standby.Spray under 50PSI pressure with Potter spray tower, every basin 5mL.Each medicament arranges 5 Concentraton gradient,
With spray equivalent clear water as blank.Spray inoculation bacterium of downy mildew of cucumber sporangia suspension, spore after chemicals treatment 24h
The configuration process of capsule suspension is as follows: take the cucumber leaves picking up from field with cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew,
Dip the Sporangium in the distillation washing lower back face of about 10 DEG C with brush pen, being made into concentration is 3 × 105The Sporangium of individual/mL suspends
Liquid.Cucumber seedling is placed in growth cabinet after inoculation (relative humidity 100%, temperature 15-20 DEG C) cultivate, after 24h, keeps temperature
Spending 15-24 DEG C, relative humidity about 90% moisturizing induction, 10d " Invest, Then Investigate " records incidence, calculates disease index and preventing and treating effect
Really.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
The Toxicity Determination of cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew is tied by table 4, benzothiostrobin and fluopicolide
Really
During from table 4, it can be seen that benzothiostrobin and fluopicolide are compounding in the range of 20:1～1:10 (by weight), altogether
Poison coefficient (CTC) is all higher than 120, and cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew is all shown synergistic function；Benzene thiophene
Bacterium ester and fluopicolide are in the range of 10:1～1:4 (by weight), and synergistic function is the most notable, co-toxicity coefficient (CTC)
All more than 200；Benzothiostrobin and fluopicolide compound with 2:1 (by weight), and synergistic function is the most notable, co-toxicity coefficient
(CTC) it is 274.79.Visible, benzothiostrobin and fluopicolide compound has reasonability and feasibility.
In sum, it is a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides that the present invention provides, collaborative
Potentiation is notable, and compared with existing unitary agent, on preventing and treating crops, the effect of disease significantly improves, and pacifies crops
Entirely, popularization and application in agricultural production it are worth.
Claims (9)
1. one kind contains benzothiostrobin and the bactericidal composition of acid amide fungicides, it is characterised in that: effective ingredient is benzene thiophene bacterium
The weight ratio of ester and acid amide fungicides, benzothiostrobin and acid amide fungicides is 20:1～1:10.
The most according to claim 1 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature exists
In: the weight ratio of benzothiostrobin and acid amide fungicides is 10:1～1:4.
The most according to claim 1 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature exists
In: described acid amide fungicides one in amisulbrom or fluopicolide.
The most according to claim 3 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature exists
In: benzothiostrobin and amisulbrom are with 4:1.
The most according to claim 3 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature exists
In: benzothiostrobin and fluopicolide are with 2:1.
The most according to claim 1 and 2 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature
It is: the weight summation of benzothiostrobin and acid amide fungicides percentage by weight in the composition is 1%～85%.
The most according to claim 1 a kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides, its feature exists
In: its dosage form is microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wettable powder and ultra-low volume
One in liquor.
8. a kind of bactericidal composition containing benzothiostrobin and acid amide fungicides described in claim 1 is to prevent and treat crops
On by the application in the microbial disease of Oomycete cause of disease.
Described a kind of bactericidal composition containing benzothiostrobin and acid amide fungicides the most according to claim 5 is
By the application in the microbial disease of Oomycete cause of disease on preventing and treating crops.It is characterized in that: described Oomycete pathogen is drawn
The disease risen is downy mildew, epidemic disease, late blight.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610522182.8A CN106172429A (en) | 2016-07-05 | 2016-07-05 | A kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides |
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| CN201610522182.8A CN106172429A (en) | 2016-07-05 | 2016-07-05 | A kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides |
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| CN106172429A true CN106172429A (en) | 2016-12-07 |
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| CN107156141A (en) * | 2017-06-05 | 2017-09-15 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition containing benzothiostrobin and zoxamide |
| CN107347896A (en) * | 2017-06-05 | 2017-11-17 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition for preventing and treating rice sheath blight disease |
| CN107484763A (en) * | 2017-09-19 | 2017-12-19 | 安徽古尔特科技有限公司 | A kind of bactericidal composition containing benzothiostrobin and Guardian |
| CN115039784A (en) * | 2021-03-08 | 2022-09-13 | 东莞市东阳光农药研发有限公司 | Composition and application thereof in agriculture |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107156141A (en) * | 2017-06-05 | 2017-09-15 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition containing benzothiostrobin and zoxamide |
| CN107347896A (en) * | 2017-06-05 | 2017-11-17 | 广西南宁乐蕊生物科技有限责任公司 | A kind of bactericidal composition for preventing and treating rice sheath blight disease |
| CN107484763A (en) * | 2017-09-19 | 2017-12-19 | 安徽古尔特科技有限公司 | A kind of bactericidal composition containing benzothiostrobin and Guardian |
| CN115039784A (en) * | 2021-03-08 | 2022-09-13 | 东莞市东阳光农药研发有限公司 | Composition and application thereof in agriculture |
| CN115039784B (en) * | 2021-03-08 | 2024-01-16 | 东莞市东阳光农药研发有限公司 | Composition and application thereof in agriculture |
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