CN106070246A - A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole - Google Patents
A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole Download PDFInfo
- Publication number
- CN106070246A CN106070246A CN201610466540.8A CN201610466540A CN106070246A CN 106070246 A CN106070246 A CN 106070246A CN 201610466540 A CN201610466540 A CN 201610466540A CN 106070246 A CN106070246 A CN 106070246A
- Authority
- CN
- China
- Prior art keywords
- bacterium azoles
- dichloro
- cycloheximide triazole
- bactericidal composition
- cycloheximide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses and a kind of dislike bacterium azoles and the bactericidal composition of cycloheximide triazole containing dichloro, dislike bacterium azoles and cycloheximide triazole and conventional auxiliary element including active component dichloro, it is 10:1～1:10 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole.Great number of indoor and outdoor experiments show that after being compounded, the bactericidal composition of the present invention multiple diseases to the growth that harms the crops, especially to by the microbial disease of Oomycete cause of disease, there is significant synergistic function, decrease Pesticide use amount simultaneously, reduce pesticide residual quantity on crops, alleviate the pollution to environment, delayed the drug-fast generation of pathogen, added the Pesticide use life-span.
Description
Technical field
The present invention relates to pesticide sterilizing composite and application thereof, specifically dislike bacterium azoles and cycloheximide triazole with dichloro
For the binary complex that active component is compounding, it is mainly used in the preventing and treating of disease on crops.
Background technology
Dichloro dislikes bacterium azoles, molecular formula: C9H6Cl2N2O3S, molecular weight 293.1, dichloro is disliked bacterium azoles and is drawn by Oomycete pathogen
The disease such as late blight, the downy mildew etc. that rise have good prevention effect.
Cycloheximide triazole (famoxadone), Mitochondrial electron transmission inhibitor, cytochrome C in complex III is aoxidized
Reductase has inhibitory action, have protection, treat, root out, permeate, systemic activity.Suitable for crop such as Semen Tritici aestivi, Fructus Hordei Vulgaris, Semen Pisi sativi, sweet
Dish, Brassica campestris L, Fructus Vitis viniferae, Rhizoma Solani tuber osi, pawl class, Fructus Capsici, Fructus Lycopersici esculenti etc..It is mainly used in preventing and treating in Ascomycetes, Basidiomycetes, oomycetes subclass
Important disease such as powdery mildew, rust, glume blight, net blotch, downy mildew, late blight etc..
Pesticide individually long-time uses continuously and easily produces resistance risk and consumption increases risk, so increase cost and
Carrying capacity of environment, and the compositions with notable synergistic function is filtered out by agriculture chemical compounding, Pesticide use can not only be reduced
Amount, the use cost that reduces, minimizing resistance risk, moreover it is possible to reduce potential environmental risk.
Summary of the invention
Disliking bacterium azoles and the bactericidal composition of cycloheximide triazole it is desirable to provide a kind of containing dichloro, this bactericidal composition has
There is significant synergistic function, can be used for multiple diseases on crops, especially by the microbial disease of Oomycete cause of disease
Preventing and treating.
Technical scheme: a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, becomes including activity
Dividing dichloro to dislike bacterium azoles and cycloheximide triazole and conventional auxiliary element, it is 10:1～1 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole:
2, dichloro dislikes bacterium azoles and cycloheximide triazole gross weight percentage composition in the composition is 1%～85%, and remaining is to allow in pesticide
Use and acceptable auxiliary element.
Preferably, dichloro dislikes bacterium azoles and cycloheximide triazole weight ratio in the composition is 8:1～1:1.
Described auxiliary element is solvent, emulsifying agent, wetting agent, dispersant, stabilizer, antifreezing agent, thickening agent, disintegrate
Agent, defoamer, penetrating agent, macromolecule cyst material, synergist, preservative and filler etc. are all conventional in pesticidal preparations or permit
Being permitted the composition used, be not particularly limited, concrete composition and consumption are determined by test according to recipe requirements.
Bactericidal composition of the present invention can be prepared as the multiple application forms being suitable in plant production using, reasonable dose
Type has microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wettable powder and cream.
The production technology of the various application forms of described bactericidal composition all belongs to existing known technology, does not repeats them here.
Bactericidal composition of the present invention can be used for multiple diseases on crops, especially by the microbial disease of Oomycete cause of disease
Preventing and treating.
Compared with prior art, it is an advantage of the current invention that:
1, dichloro dislikes bacterium azoles and cycloheximide triazole compounds and has significant synergistic function, is used alone substantially than single dose
Improve the prevention effect of disease on crops.
2, dichloro dislikes bacterium azoles and two kinds of active component of cycloheximide triazole compound, and decreases Pesticide use amount, reduces and use into
This, alleviate the pollution to environment.
3, dichloro dislikes bacterium azoles and two kinds of active component of cycloheximide triazole compound, and the mechanism of action is different, and action site increases,
Delay the drug-fast generation of pathogen, add the service life of pesticide.
Detailed description of the invention
In order to be more fully understood that the essence of the present invention, below in conjunction with embodiment, present disclosure is described further,
But can not be considered as limitation of the present invention, the following stated is only used for explaining the present invention, for without departing from present invention spirit and principle
The amendment made, replace or improve, belong to the scope of protection of present invention.
Embodiment one: 20% dichloro dislikes bacterium azoles cycloheximide triazole microcapsule suspending agent.
Following material is all weight percentage: dichloro dislike bacterium azoles 10%, cycloheximide triazole 10%, toluene diacetonitrile acid esters 3%,
Sodium carboxymethyl cellulose 2%, trimethylbenzene 10%, fatty acid methyl ester 10%, castor oil polyoxyethylene ether 5%, maleic-acrylic acid
Copolymer sodium salt 3%, sodium hydroxide 2%, deionized water complements to 100%.By above-mentioned active component, macromolecule cyst material,
Solvent mixes, and makes to be dissolved into homogeneous oil phase, in a shear condition, joins oil phase containing emulsifying agent, dispersant, pH adjusting agent
Aqueous phase solution in, surplus deionized water is supplied, and bi-material reacts at oil-water interfaces, formed macromolecule cyst wall, i.e.
20% dichloro can be prepared and dislike bacterium azoles cycloheximide triazole microcapsule suspending agent.
This embodiment is used for preventing and treating the late blight of potato: 20% dichloro dislikes bacterium azoles cycloheximide triazole microcapsule suspending agent by 150g
A.i./ha consumption carries out dispenser for the first time at late blight of potato their early stage, carries out second time dispenser, separately set at clear water after 7d
Reason is blank, and after second time dispenser, 14d is 96.04% to the preventive effect of the late blight of potato；20% dichloro is disliked bacterium azoles and is hanged
Floating agent carries out dispenser for the first time by 120g a.i./ha consumption at late blight of potato their early stage, carries out second time and execute after 7d
Medicine, separately sets clear water and is processed as blank, and after second time dispenser, 14d is 73.49% to the preventive effect of the late blight of potato；50%
Cycloheximide triazole water dispersible granules carries out dispenser for the first time by 150g a.i./ha consumption at late blight of potato their early stage, after 7d
Carrying out second time dispenser, separately set clear water and be processed as blank, after second time dispenser, the preventive effect of the late blight of potato is by 14d
77.42%.Visible, dichloro dislikes obvious synergy after bacterium azoles and cycloheximide triazole binary built, and the lasting period is long, to Rhizoma Solani tuber osi
The preventive effect of late blight is used alone significantly better than each single dose, meanwhile, does not finds that this binary built medicament is to horse in process of the test
Bell potato produces poisoning.
Embodiment two: 24% dichloro dislikes bacterium azoles cycloheximide triazole suspending agent.
Following material is all weight percentage: dichloro dislikes bacterium azoles 12%, cycloheximide triazole 12%, alkylphenol-polyethenoxy base ether
Formaldehyde condensation products sulfate 10%, cetyl diphenyl ether sodium disulfonate 5%, phenethyl phenol polyethenoxy base ether phosphate 5%,
Ethylene glycol 3%, xanthan gum 1%, polyhydroxy benzenes propyl formate 2%, C10-20 saturated fat acid compounds 0.1%, deionization
Water supplies 100%.By above-mentioned active component, wetting agent, dispersant, antifreezing agent, thickening agent, preservative and defoamer through cutting at a high speed
Cutting dissipates, sand milling in sand mill, and the high dispersive formed in water-medium, stable suspension system can be prepared by 24% dichloro
Dislike bacterium azoles cycloheximide triazole suspending agent.
This embodiment is used for preventing and treating cucumber downy mildew: 24% dichloro dislike bacterium azoles cycloheximide triazole suspending agent by 150g a.i./
Ha consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 93.17% to the preventive effect of cucumber downy mildew；20% dichloro is disliked bacterium azole suspending agent and is pressed
120g a.i./ha consumption carries out dispenser for the first time at cucumber downy mildew their early stage, carries out second time dispenser after 7d, separately sets clear
Water is processed as blank, and after second time dispenser, 14d is 71.35% to the preventive effect of cucumber downy mildew；50% cycloheximide triazole water
Dispersible granule carries out dispenser for the first time by 150g a.i./ha consumption at cucumber downy mildew their early stage, carries out second time and execute after 7d
Medicine, separately sets clear water and is processed as blank, and after second time dispenser, 14d is 75.91% to the preventive effect of cucumber downy mildew.It is visible,
Dichloro dislikes obvious synergy after bacterium azoles and cycloheximide triazole binary built, and the lasting period is long, bright to the preventive effect of cucumber downy mildew
Aobvious it be better than each single dose and be used alone, meanwhile, in process of the test, do not find that this binary built medicament produces poisoning to Fructus Cucumidis sativi.
Embodiment three: 30% dichloro dislikes bacterium azoles cycloheximide triazole aqueous emulsion.
Following material is all weight percentage: dichloro dislike bacterium azoles 20%, cycloheximide triazole 10%, Methyl palmitoleinate 10%,
Ketohexamethylene 5%, polyol fatty acid ester and ethylene oxide adduct 10% thereof, fatty acid glyceride polyoxyethylene ether 3%, methyl
The formaldehyde condensation products 5% of sodium naphthalene sulfonate, acetic anhydride 1%, glycerol 1%, deionized water supplies 100%.By above-mentioned active component, molten
Agent, emulsifying agent, dispersant and stabilizer are mixed to form oil phase, and antifreezing agent dissolves formation aqueous phase in water, under cutter effect
Slowly oil phase is added in aqueous phase, and continue to shear about 30 minutes, prepare 30% dichloro and dislike bacterium azoles cycloheximide triazole aqueous emulsion.
This embodiment is used for preventing and treating tomato late blight: 30% dichloro dislike bacterium azoles cycloheximide triazole aqueous emulsion by 150g a.i./
Ha consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 98.36% to the preventive effect of tomato late blight；20% dichloro is disliked bacterium azole suspending agent and is pressed
120g a.i./ha consumption carries out dispenser for the first time at tomato late blight their early stage, carries out second time dispenser after 7d, separately sets clear
Water is processed as blank, and after second time dispenser, 14d is 79.41% to the preventive effect of tomato late blight；50% cycloheximide triazole water
Dispersible granule carries out dispenser for the first time by 150g a.i./ha consumption at tomato late blight their early stage, carries out second time and execute after 7d
Medicine, separately sets clear water and is processed as blank, and after second time dispenser, 14d is 78.58% to the preventive effect of tomato late blight.It is visible,
Dichloro dislikes obvious synergy after bacterium azoles and cycloheximide triazole binary built, and the lasting period is long, bright to the preventive effect of tomato late blight
Aobvious it be better than each single dose and be used alone, meanwhile, in process of the test, do not find that this binary built medicament produces poisoning to Fructus Lycopersici esculenti.
Embodiment four: 45% dichloro dislikes bacterium azoles cycloheximide triazole microemulsion.
Following material is all weight percentage: dichloro dislikes bacterium azoles 5%, cycloheximide triazole 40%, Methyl palmitoleinate 10%, ring
Hexanone 5%, triphen ethyl phenol polyoxyethylene groups ether phosphate 10%, fatty alcohol-polyoxyethylene ether 5%, triphenyl phosphite 2%,
Organosilicon 1%, propyl isome 2%, deionized water supplies 100%.Active component solvent is completely dissolved, then adds emulsifying
Agent, stabilizer, penetrating agent, synergist mix homogeneously, be eventually adding deionized water, after being sufficiently stirred for, can be prepared by 45% dichloro
Dislike bacterium azoles cycloheximide triazole microemulsion.
This embodiment is used for preventing and treating downy mildew of garpe: 45% dichloro dislike bacterium azoles cycloheximide triazole microemulsion by 150g a.i./
Ha consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser, separately set clear water and be processed as sky after 7d
White comparison, after second time dispenser, 14d is 94.27% to the preventive effect of downy mildew of garpe；20% dichloro is disliked bacterium azole suspending agent and is pressed
120g a.i./ha consumption carries out dispenser for the first time at downy mildew of garpe their early stage, carries out second time dispenser after 7d, separately sets clear
Water is processed as blank, and after second time dispenser, 14d is 73.89% to the preventive effect of downy mildew of garpe；50% cycloheximide triazole water
Dispersible granule carries out dispenser for the first time by 150g a.i./ha consumption at downy mildew of garpe their early stage, carries out second time and execute after 7d
Medicine, separately sets clear water and is processed as blank, and after second time dispenser, 14d is 71.13% to the preventive effect of downy mildew of garpe.It is visible,
Dichloro dislikes obvious synergy after bacterium azoles and cycloheximide triazole binary built, and the lasting period is long, bright to the preventive effect of downy mildew of garpe
Aobvious it be better than each single dose and be used alone, meanwhile, in process of the test, do not find that this binary built medicament produces poisoning to Fructus Vitis viniferae.
Embodiment five: 30% dichloro dislikes bacterium azoles cycloheximide triazole cream.
Following material is all weight percentage: dichloro dislikes bacterium azoles 22%, cycloheximide triazole 8%, trimethylbenzene 10%, Ketohexamethylene
5%, triphen ethyl phenol polyoxyethylene ether phosphate 5%, alkylphenol-polyethenoxy polyethenoxy ether 20%, double to kill 2%, Lignum Pini Nodi
Oil 5%, solvent naphtha complements to 100%.Fully dissolve after above-mentioned active component is added solvent, be subsequently adding emulsifying agent, potentiation
Agent becomes the oily liquids of transparent and homogeneous after stirring, can be prepared by 30% dichloro and dislike bacterium azoles cycloheximide triazole cream.
This embodiment is used for preventing and treating Citrullus vulgaris epidemic disease: 30% dichloro is disliked bacterium azoles cycloheximide triazole cream and used by 150g a.i./ha
Measure and carry out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carry out second time dispenser after 7d, separately set clear water and be processed as blank,
After second time dispenser, 14d is 96.34% to the preventive effect of Citrullus vulgaris epidemic disease；20% dichloro is disliked bacterium azole suspending agent and is pressed 120g a.i./ha
Consumption carries out for the first time dispenser at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser after 7d, separately set clear water be processed as blank right
According to, after second time dispenser, 14d is 72.81% to the preventive effect of Citrullus vulgaris epidemic disease；50% cycloheximide triazole water dispersible granules presses 150g
A.i./ha consumption carries out dispenser for the first time at Citrullus vulgaris epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 78.92% to the preventive effect of Citrullus vulgaris epidemic disease.Visible, dichloro dislikes bacterium azoles and cycloheximide triazole
Obvious synergy after binary built, the lasting period is long, is used alone the preventive effect of Citrullus vulgaris epidemic disease significantly better than each single dose, with
Time, in process of the test, do not find that this binary built medicament produces poisoning to Citrullus vulgaris.
Embodiment six: 70% dichloro dislikes bacterium azoles cycloheximide triazole water dispersible granules.
Following material is all weight percentage: dichloro dislikes bacterium azoles 20%, cycloheximide triazole 50%, polyoxyethylene tri-styryl benzene
Based phosphates 5%, sodium acrylate and propionic acid amide. copolymer 5%, carbamide 5%, precipitated calcium carbonate 5%, kieselguhr supplies 100%.
By above-mentioned effective ingredient, wetting agent, dispersant, disintegrating agent and filler mix homogeneously, pulverize with micro jet, through pinching
Close, be subsequently adding in fluidized-bed granulation dryer and carry out pelletize, dry, screening, can be prepared by 70% dichloro and dislike bacterium azoles azoles
Bacterium ketone water dispersible granules.
This embodiment is used for preventing and treating capsicum epidemic disease: 70% dichloro dislikes bacterium azoles cycloheximide triazole water dispersible granules by 150g
A.i./ha consumption carries out dispenser for the first time at capsicum epidemic disease their early stage, carries out second time dispenser, separately set clear water and be processed as after 7d
Blank, after second time dispenser, 14d is 91.24% to the preventive effect of capsicum epidemic disease；20% dichloro is disliked bacterium azole suspending agent and is pressed
120g a.i./ha consumption carries out dispenser for the first time at capsicum epidemic disease their early stage, carries out second time dispenser, separately set clear water after 7d
Being processed as blank, after second time dispenser, 14d is 70.87% to the preventive effect of capsicum epidemic disease；50% cycloheximide triazole is water-dispersible
Granule carries out dispenser for the first time by 150g a.i./ha consumption at capsicum epidemic disease their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 75.12% to the preventive effect of capsicum epidemic disease.Visible, dichloro dislikes bacterium
Obvious synergy after azoles and cycloheximide triazole binary built, the lasting period is long, and the preventive effect of capsicum epidemic disease is significantly better than each list
Agent is used alone, and meanwhile, does not finds that this binary built medicament produces poisoning to Fructus Capsici in process of the test.
Embodiment seven: 80% dichloro dislikes bacterium azoles cycloheximide triazole wettable powder.
Following material is all weight percentage: dichloro dislikes bacterium azoles 50%, cycloheximide triazole 30%, polyoxyethylene tri-styryl benzene
Based phosphates 5%, sodium acrylate and propionic acid amide. copolymer 5%, white carbon 5%, kieselguhr supplies 100%.By above-mentioned by effectively
Composition, wetting agent, dispersant and filler mixing, again through comminution by gas stream, mix homogeneously after mechanical activation comminution, can be prepared by 80% dichloro
Dislike bacterium azoles cycloheximide triazole wettable powder.
This embodiment views and admires Flos Chrysanthemi downy mildew for preventing and treating: 80% dichloro is disliked bacterium azoles cycloheximide triazole wettable powder and pressed
150g a.i./ha consumption carries out for the first time dispenser viewing and admiring Flos Chrysanthemi downy mildew their early stage, carries out second time dispenser, separately after 7d
If clear water is processed as blank, after second time dispenser, 14d is 97.08% to the preventive effect viewing and admiring Flos Chrysanthemi downy mildew；20% 2
Chlorine is disliked bacterium azole suspending agent and is carried out for the first time dispenser by 120g a.i./ha consumption viewing and admiring Flos Chrysanthemi downy mildew their early stage, and 7d is laggard
Row second time dispenser, separately sets clear water and is processed as blank, and after second time dispenser, the preventive effect viewing and admiring Flos Chrysanthemi downy mildew is by 14d
75.32%；50% cycloheximide triazole water dispersible granules is carried out viewing and admiring Flos Chrysanthemi downy mildew their early stage by 150g a.i./ha consumption
Dispenser for the first time, carries out second time dispenser, separately sets clear water and be processed as blank after 7d, after second time dispenser, 14d is to viewing and admiring
The preventive effect of Flos Chrysanthemi downy mildew is 74.66%.Visible, after dichloro dislikes bacterium azoles and cycloheximide triazole binary built, synergistic function shows
Writing, the lasting period is long, is used alone, significantly better than each single dose, the preventive effect viewing and admiring Flos Chrysanthemi downy mildew, meanwhile, in process of the test not
Find that this binary built medicament produces poisoning to viewing and admiring Flos Chrysanthemi.
Embodiment eight: dichloro dislikes bacterium azoles and the compounding Toxicity Determination to late blight of potato pathogenic bacterium of cycloheximide triazole is tried
Test.
Test target: late blight of potato pathogenic bacterium phytophthora infestans (Phytophthora infestans)
Test uses mycelial growth rate method with reference to " farm-chemical indoor determination test rule NY/T1156.2-2006 ".
Take the medicinal liquid that 5mL prepares join equipped with the hot culture medium of 45mL (rye (Secale cereale L.) culture medium, 45-50 DEG C.Rye (Secale cereale L.) culture medium prescription: black
Wheat 60g, sucrose 20g, agar powder 12g, add water to 1000mL) conical flask in, after shaking up, pour into rapidly diameter 90mm glass training
Supporting ware, each culture dish is poured band medicine culture medium 10mL into, is compared with the sterile water solution containing appropriate acetone and Tween 80,
Each process 4 repetition, horizontal rest, make flat board after cooling.Every ware accesses the bacterium cake one of diameter 0.4cm.All operations
All carry out sterile working at superclean bench.It is placed on dark culturing in the constant incubator of 20 DEG C after process, takes out after 10d.Adopt
Measure and record the colony diameter of each process by decussation method, calculate suppression ratio.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
Table 1, dichloro are disliked bacterium azoles and cycloheximide triazole and are tied the Toxicity Determination of late blight of potato pathogenic bacterium phytophthora infestans
Really
| Medicament title and proportioning (weight ratio) | EC<sub>50</sub>(mg/L) | ATI | TTI | CTC |
| Dichloro dislikes bacterium azoles | 3.13 | 100.00 | - | - |
| Cycloheximide triazole | 5.34 | 58.61 | - | - |
| Dichloro evil bacterium azoles: cycloheximide triazole (10:1) | 1.79 | 174.86 | 96.24 | 181.69 |
| Dichloro evil bacterium azoles: cycloheximide triazole (7:1) | 1.67 | 187.43 | 94.83 | 197.65 |
| Dichloro evil bacterium azoles: cycloheximide triazole (5:1) | 1.53 | 204.58 | 93.10 | 219.74 |
| Dichloro evil bacterium azoles: cycloheximide triazole (3:1) | 1.41 | 221.99 | 89.65 | 247.62 |
| Dichloro evil bacterium azoles: cycloheximide triazole (1:1) | 1.38 | 226.81 | 79.31 | 285.98 |
| Dichloro evil bacterium azoles: cycloheximide triazole (1:3) | 1.72 | 181.98 | 68.96 | 263.89 |
| Dichloro evil bacterium azoles: cycloheximide triazole (1:5) | 2.08 | 150.48 | 65.51 | 229.71 |
| Dichloro evil bacterium azoles: cycloheximide triazole (1:7) | 2.57 | 121.79 | 63.78 | 190.95 |
| Dichloro evil bacterium azoles: cycloheximide triazole (1:10) | 2.85 | 109.82 | 62.37 | 176.08 |
During as it can be seen from table 1 dichloro evil bacterium azoles and cycloheximide triazole are compounding in the range of 10:1～1:10 (by weight),
Co-toxicity coefficient (CTC) is all higher than 120, and late blight of potato pathogenic bacterium phytophthora infestans is all shown synergistic function；Dichloro
Evil bacterium azoles and cycloheximide triazole are in the range of 5:1～1:5 (by weight), and synergistic function is the most notable, co-toxicity coefficient (CTC)
All more than 200；Dichloro is disliked bacterium azoles and cycloheximide triazole and is compounded with 1:1 (by weight), and synergistic function is the most notable, altogether poison system
Number (CTC) is 285.98.Visible, dichloro dislikes bacterium azoles and cycloheximide triazole compounds and has reasonability and feasibility.
Embodiment nine: dichloro dislikes bacterium azoles and the compounding Toxicity Determination to cucumber downy mildew pathogenic bacterium of cycloheximide triazole is tried
Test.
Test target: cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew (Pseudoperonosporacubensis)
This test uses pot-culture method.Choosing the two panels leaf period cucumber seedling that growing way is consistent, each process selects 5 basins for examination melon
Seedling, numbers standby.Spray under 50PSI pressure with Potter spray tower, every basin 5mL.Each medicament arranges 5 Concentraton gradient,
With spray equivalent clear water as blank.Spray inoculation bacterium of downy mildew of cucumber sporangia suspension, spore after chemicals treatment 24h
The configuration process of capsule suspension is as follows: take the cucumber leaves picking up from field with cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew,
Dip the Sporangium in the distillation washing lower back face of about 10 DEG C with brush pen, being made into concentration is 3 × 105The Sporangium of individual/mL suspends
Liquid.Cucumber seedling is placed in growth cabinet after inoculation (relative humidity 100%, temperature 15-20 DEG C) cultivate, after 24h, keeps temperature
Spending 15-24 DEG C, relative humidity about 90% moisturizing induction, 10d " Invest, Then Investigate " records incidence, calculates disease index and preventing and treating effect
Really.
Carry out statistical analysis with DPS statistical analysis software, calculate the EC of each medicament50, and calculate mixture according to the abundant method of Sun Yun
Co-toxicity coefficient (CTC value).
Actual measurement toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100。
Percentage composition+B medicament the toxicity index of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture × mixed
The percentage composition of B in agent.
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theory toxicity index (TTI)] × 100.
According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC) >=120 shows as increasing
Effect effect；Co-toxicity coefficient (CTC)≤80 shows as antagonism；80 ＜ co-toxicity coefficient (CTC) ＜ 120 show as summation action.
Table 2, dichloro dislike bacterium azoles and the cycloheximide triazole Toxicity Determination to cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew
Result
During from table 2 it can be seen that dichloro evil bacterium azoles and cycloheximide triazole are compounding in the range of 10:1～1:10 (by weight),
Co-toxicity coefficient (CTC) is all higher than 120, and cucumber downy mildew pathogenic bacterium Cuba artificial downy mildew is all shown synergistic function；Two
Chlorine evil bacterium azoles and cycloheximide triazole are in the range of 5:1～1:5 (by weight), and synergistic function is the most notable, co-toxicity coefficient
(CTC) all more than 200；Dichloro is disliked bacterium azoles and cycloheximide triazole and is compounded with 1:1 (by weight), and synergistic function is the most notable,
Co-toxicity coefficient (CTC) is 276.74.Visible, dichloro dislikes bacterium azoles and cycloheximide triazole compounds and has reasonability and feasibility.
In sum, what the present invention provided is a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, collaborative increasing
Effect effect is notable, and compared with existing unitary agent, on preventing and treating crops, the effect of disease significantly improves, to Crop securify,
It is worth popularization and application in agricultural production.
Claims (7)
1. dislike bacterium azoles and the bactericidal composition of cycloheximide triazole containing dichloro for one kind, it is characterised in that: include that active component dichloro is disliked
Bacterium azoles and cycloheximide triazole, it is 10:1～1:2 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole.
It is the most according to claim 1 a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, it is characterised in that:
It is 8:1～1:1 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole ketone.
It is the most according to claim 1 a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, it is characterised in that:
It is 1:1 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole ketone.
It is the most according to claim 1 a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, it is characterised in that:
It is 3:1 that dichloro dislikes the weight ratio of bacterium azoles and cycloheximide triazole ketone.
It is the most according to claim 1 a kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole, it is characterised in that:
Dichloro dislikes bacterium azoles and cycloheximide triazole gross weight percentage composition in the composition is 1%～85%, and remaining is for allowing to make in pesticide
With with acceptable auxiliary element.
6. dislike bacterium azoles and the bactericidal composition of cycloheximide triazole according to a kind of described in claim 1,2,3,4,5 or 6 containing dichloro,
It is characterized in that: the dosage form of described bactericidal composition be microcapsule suspending agent, suspending agent, aqueous emulsion, microemulsion, water dispersible granules,
One in wettable powder and cream.
7. described in claim 1 a kind of containing dichloro dislike the bactericidal composition of bacterium azoles and cycloheximide triazole on preventing and treating crops by
The purposes of the microbial disease of Oomycete cause of disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610466540.8A CN106070246A (en) | 2016-06-21 | 2016-06-21 | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610466540.8A CN106070246A (en) | 2016-06-21 | 2016-06-21 | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106070246A true CN106070246A (en) | 2016-11-09 |
Family
ID=57252682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610466540.8A Withdrawn CN106070246A (en) | 2016-06-21 | 2016-06-21 | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106070246A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689148A (en) * | 2016-12-28 | 2017-05-24 | 佛山市普尔玛农化有限公司 | Pesticide composition containing dichloroxazole and dimethomorph |
| CN107318856A (en) * | 2017-08-17 | 2017-11-07 | 佛山市盈辉作物科学有限公司 | Bactericidal composition comprising Er Lv Evil bacterium azoles and copper 8-hydroxyquinolinate |
-
2016
- 2016-06-21 CN CN201610466540.8A patent/CN106070246A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106689148A (en) * | 2016-12-28 | 2017-05-24 | 佛山市普尔玛农化有限公司 | Pesticide composition containing dichloroxazole and dimethomorph |
| CN107318856A (en) * | 2017-08-17 | 2017-11-07 | 佛山市盈辉作物科学有限公司 | Bactericidal composition comprising Er Lv Evil bacterium azoles and copper 8-hydroxyquinolinate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104886073B (en) | A kind of microbicide compositions and preparation and its application | |
| CN104488870B (en) | A kind of composition pesticide containing bromothalonil | |
| CN102630679B (en) | Pesticide composition containing orysastrobin | |
| CN104872136B (en) | A kind of microbicide compositions and preparation and its application | |
| CN106172429A (en) | A kind of containing benzothiostrobin with the bactericidal composition of acid amide fungicides | |
| CN106106470A (en) | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of amisulbrom | |
| CN106070246A (en) | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of cycloheximide triazole | |
| CN104542585A (en) | Fungicide composition and application thereof | |
| CN106172435A (en) | A kind of containing benzothiostrobin with the bactericidal composition of fluorine thiazole pyrrole ethyl ketone | |
| CN106070241A (en) | A kind of containing dichloro evil bacterium azoles and the bactericidal composition of fluorine thiazole pyrrole ethyl ketone | |
| CN103081920B (en) | Sterilizing composition containing penconazole | |
| CN103636668B (en) | Sterilization composition containing Dufulin and orysastrobin | |
| CN109548787A (en) | A kind of bactericidal composition and its application | |
| CN106689148A (en) | Pesticide composition containing dichloroxazole and dimethomorph | |
| CN107242242A (en) | Bactericidal composition comprising folpet and prothioconazoles | |
| CN107801720A (en) | Bactericidal composition and its application | |
| CN103636670B (en) | Sterilization composition containing Dufulin and enestroburin | |
| CN104970021A (en) | Sterilization composition containing oxine-copper and xinjunan acetate | |
| CN104012555A (en) | Agricultural sterilization composition | |
| CN104920359B (en) | Plant essential oil for preventing and controlling rice blast efficiently | |
| CN104920358B (en) | Natural product combination for preventing and controlling rice blast | |
| CN109717195A (en) | A kind of bactericidal composition for disliking bacterium azoles and zoxamide containing dichloro | |
| CN107960416A (en) | A kind of bactericidal composition and its application containing dimethomorph | |
| CN105994291A (en) | Bactericidal composition containing benzovindiflupyr | |
| CN103798291B (en) | A kind of Recompounded pesticide containing triazolone and Cupravit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20161109 |
|
| WW01 | Invention patent application withdrawn after publication |