CN104255293B - A kind of large-scale live body grafting glossy ganoderma dish garden cultivation base combination and management technique - Google Patents
A kind of large-scale live body grafting glossy ganoderma dish garden cultivation base combination and management technique Download PDFInfo
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Abstract
The invention discloses the combination of a kind of large-scale live body grafting glossy ganoderma dish garden cultivation base and management technique, comprise the following steps: (1) selects strain；(2) inoculation；(3) bacterium is sent out；(4) combination: after bacterium bag is sent out completely, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into bulk and puts in sack, be inside compacted, when reaching preset height, has i.e. accused the combination of a cultivation base by sack sealing, after every bag of combination, if prick dry hole at angle, base；(5) management after combination.The present invention is by fast oxygenation and avoids a bag internal heat generation, it is achieved the cultivation base combination of large-scale glossy ganoderma dish garden, it is possible to cultivate large-scale glossy ganoderma dish garden.When overcoming combination in prior art, several bacterium bag peelings are placed in sack, owing to anoxia causes interior-heat bacterium bag miscellaneous bacteria to infect and the overheated defect that should not cultivate large-scale live body glossy ganoderma dish garden.Especially this technique can cultivate live body glossy ganoderma dish garden large-scale, the most oversized.
Description
Technical field
The present invention relates to the combination of a kind of large-scale live body grafting glossy ganoderma dish garden cultivation base and management technique, belong to bacterium industry cultivation field.
Background technology
Ganoderma, has another name called Herba mesonae chinensis, SHENCAO, Ganoderma, is famous and precious Chinese herbal medicine.It is the rarity in Pharmacopoea Chinensis, there is magical effect the most eliminating evil, that come back to life, enjoy the highest praise at all times.The symbol that Ganoderma is still had good luck, has saying of " thousand Ganoderma a century fortune ", and ancient times are rare because of it, and emperors and ministers high official and noble lord make as one wishes according to the shape of Ganoderma, be placed in hall desk pray for blessings receive auspicious.
The most so-called live body grafting Ganoderma views and admires potted landscape, i.e. Ganoderma uses artificial grafting to form during growing in good time and views and admires Ganoderma.Live body Ganoderma grafting potted landscape plant type size is critically depend on size and the quality of cultivation base, and cultivation base is the biggest and disease-free, and the glossy ganoderma dish garden plant type that growth grafting goes out is the biggest, and moulding is the most attractive in appearance.And the restriction of big cultivation base each side condition is impossible one-time formed, it is necessary to after little bacterium bag mycelia sends out completely, be combined into the big bacterium bag of suitable cultivation time suitable.Then, through the management of science, after mycelia affinity, can budding Ganoderma.
Summary of the invention
Present invention seek to address that one of technical problem that above-mentioned prior art exists, it is provided that the combination of a kind of large-scale live body grafting glossy ganoderma dish garden cultivation base and management technique.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of large-scale live body glossy ganoderma dish garden cultivation base combination and management technique, comprise the following steps:
(1) strain is selected；
(2) inoculation: by selected strain to through sterilizing, load Nutrient medium bacterium bag inoculate；
(3) bacterium is sent out: carry out postvaccinal bacterium bag sending out bacterium；
(4) combination: after bacterium bag is sent out completely, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into bulk and puts in sack, be inside compacted, when reaching preset height, has i.e. accused the combination of a cultivation base by sack sealing, after every bag of combination, if prick dry hole at angle, base；
(5) management after combination.
Large-scale live body glossy ganoderma dish garden cultivation base combination according to the present invention and management technique, send out the little bacterium bag after bacterium completes and be in anaerobic condition, bacterium rod is broken into bulk and puts in sack, in sack, amount of oxygen is abundant, add to add and prick hole oxygen supplement at angle, sack base (bottom), amount of oxygen quickly fills, the quick affinity of mycelia.Additionally the cultivation base in sack is relatively big due to volume, easily generates heat and causes cultivation base pathological changes, be easy to " breathing " of thalline by pricking hole, in time release " respiration heat ", it is to avoid " interior burning " i.e. bag internal heat generation in bag.The present invention is by fast oxygenation and avoids heating, it is achieved the cultivation base combination of large-scale glossy ganoderma dish garden, it is possible to cultivate large-scale glossy ganoderma dish garden.Several bacterium bag peelings are placed in sack, owing to anoxia and overheated interior burning are not suitable for cultivating the defect of large-scale live body glossy ganoderma dish garden when overcoming combination in prior art.Especially this technique can cultivate live body glossy ganoderma dish garden large-scale, the most oversized.
Sack of the present invention refers to that volume is more than the bacterium bag sending out bacterium bacterium bag, sends out the polyethylene/polypropylene bacterium bag that bacterium bacteria used thereby bag generally uses book size to be 38cm × wide 17.5 or 18cm, therefore the volume of general super-huge bacterium bag sends out the volume of bacterium bacterium bag up to more than 50 times.Described " preset height " refer generally to combination after cultivation base height between 35-40cm, super-huge up to 40-45cm.Described " compacting " refers to the bacterium rod that will break into bulk as far as possible space between compacting block and block in the case of not destroying block structure, lumpy sizes (greatest length) between 3-8cm, the volume of cultivation base after combination: the volume sum=1.2-1.4 of bacterium rod before combination.Described " angle, base " refers to lower end, sack side and bottom surface periphery.Described " large-scale " refers to that the cultivation base length after combination is the cultivation base of more than 200cm at more than 100cm, preferably " super-huge ".The combination of large-scale live body glossy ganoderma dish garden cultivation base and management technique, described " grafting " refers to that, for " grafting " glossy ganoderma dish garden, processing step can not include grafting step.
It addition, the combination of large-scale live body glossy ganoderma dish garden cultivation base and management technique according to embodiments of the present invention can also have a following additional technical feature:
Preferably, the mould with dismountable four walls is used when described step (4) combination loads sack, spreading thin film in mould, thin film extends up to mould place suitable for reading, forms described sack, bacterium rod is broken into bulk and puts in sack, and be inside compacted, when reaching preset height, after leveling upper surface, the surrounding of thin film is covered above, shed mould, if dry hole is pricked at angle, base.It follows that pass through special die, it is simple to compacting, it is simple to make cultivation base large-scale, super-huge.
Preferably, the Ganoderma lucidum cultivation medium after described step (4) described combination is long 200-300cm × wide 50-60cm × high 45cm.In described step (4) combination, thin film is long 330cm × wide 200cm.
Described step (4) bacterium bag also includes the step of cooling after sending out completely, bacterium bag cooling 3-5 DEG C after sending out completely, broken bag combination again in 1-2 days.The temperature that bacterium is low is reduced, in sack inner heat after preventing resistance from closing by cooling.
Preferably, described step (4) combination is carried out in composed chamber, and before combination, reply composed chamber carries out disinfection, remove impurity bacterium of going out；Bacterium bag is entered composed chamber, sprays little bacterium bag two ends sterilizing with gram mould clean diluent, or Burdick lamp sterilization is installed；
Preferably, after staff washes one's hands with potassium permanganate diluent before entering the room during described step (4) combination, wear the working clothing sterilized, put on one's gloves, mask, footmuff, then enter composed chamber's operation；First one tying of bacterium bag is cut off with sterilization shears, then bacterium bag is scratched downwards along clip sterilization cutter, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into egg size bulk and puts in sack, put while be compacted, in time highly reaching preset height, sack sealing has been accused the combination of a cultivation base；To examine the bacterium rod hyphal development situation after each peeling in anabolic process, as found with disease or doubtful disease, whole bacterium rod to isolate or to destroy, and must not put in sack；If it find that half-cooked bacterium is excellent, half-cooked bacterium rod all be rejected；Staff once touches disease carrying germ rod, row operation again after disinfecting in alcohol in time.
Preferably, described step (2) Middle nutrition base includes that the raw material of following weight portion is made: wood flour 60-70 part, corn cob 15-25 part, Semen Maydis flour 10-12 part, Testa Tritici 10-12 part, Gypsum Fibrosum 1-1.5 part, quick lime 1-1.2 part, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 60-65% of Nutrient medium gross weight.Being different from medicinal lucid ganoderma owing to viewing and admiring bonsai lucid ganoderma, its degree of lignification is the highest, i.e. hardness is the highest, and potted landscape is the most firm, is just less susceptible to damage in carrying transportation.Therefore, the size of its degree of lignification is its key factor as potted landscape.
Preferably, described step (2) Middle nutrition base includes that the raw material of following weight portion is made: wood flour 60 parts, corn cob 15 parts, Semen Maydis flour 10 parts, 12 parts of Testa Tritici, 1.2 parts of Gypsum Fibrosum, quick lime 1.2 parts, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 60-65% of Nutrient medium gross weight.
Preferably, described step (3) is sent out bacterium: after inoculation, bacterium bag is moved to educate bacterium room added, put neatly；
1. temperature control: send out bacterium early stage, educates bacterium indoor temperature at 22 DEG C-26 DEG C, and relative humidity is 50-60%, notes observing the temperature difference and educating bacterium room upper and lower temperature difference, and the temperature difference should not be excessive, tends to constant temperature optimal；
2. translation frame: carried out translation frame every 8-12 days, when turning over, undercarriage to be gone up exchanges, and during translation frame, rejects dysplasia and disease bag in time, reduces polluter；
3. ventilate oxygen supplement；Educate bacterium indoor and answer timing ventilation ventilating oxygen；Educate bacterium indoor temperature suitable in the case of, if mycelial growth is slow, then prick hole oxygen supplement with sterilization needle set；
4. lucifuge: educate bacterium room and be prone to hyphal development growth under conditions of unglazed.
Preferably, management is combined to budding for cultivation base the management in sesame stage in described step (5)；
1. temperature control: after cultivation base combination, in warmhouse booth, the temperature of optimum mycelia affinity is 10-26 DEG C, preferably 20-26 DEG C；
2. control light: cultivation base combination one week after inscattering light intensity of illumination 1500-2000LX, after the 7-10 days whole affinities of mycelia, scattered light intensity of illumination 2500-3000LX will be adjusted in time, promote cultivation base to budding speed；
3. oxygen is controlled: little bacterium rod is broken to be loaded in sack, amount of oxygen own is abundant, now should airtight greenhouse production booth vent 35 days, sesame will be gone out after the whole affinity of cultivation base mycelia when buddingging, every day, partial ventilation mouth ventilation oxygen supplement was opened in timing, promoted that cultivation base is quickly buddingged；
4. prevention and control disease: the large-scale cultivation base after combination, every three days of front first quarter moon to canopy body, top, ground spray once, medicinal liquid sterilization；As found, cultivation base has yellow spotting phenomenon, cuts out in time with disinfection tool except and sterilizes affected part；White mould contamination, destroy in time in this way；Penicillium pollution in this way, quarantines, and the most serious can also go out sesame.
The additional aspect of the present invention and advantage will part be given in the following description, and part will become apparent from the description below, or is recognized by the practice of the present invention.
Detailed description of the invention
The one large-scale live body grafting glossy ganoderma dish garden cultivation base combination of the present invention and management technique, its technical parameter requires to be illustrated with Examples below.
Embodiment:A kind of large-scale live body grafting glossy ganoderma dish garden cultivation base combination and management technique, comprise the following steps:
(1) strain is selected
Selected conversion ratio, disease-resistant energy are strong, and the strain being suitable for potted landscape growth and graft modeling is the first step doing glossy ganoderma dish garden cultivation well.Select strain, nothing more than three kinds of forms: one with blade be main be shaped to excellent；Two with handle dry be main be shaped to excellent；Three is that petiole moulding is the most excellent.The present embodiment uses Ganoderma lucidum (Leyss. Ex Fr.) Karst..
(2) inoculation: the bacterium bag loading Nutrient medium is inoculated by selected strain.Need the bacterium bag to loading Nutrient medium need to be placed on normal-pressure sterilization 24 hours in autoclave/pond before inoculation.
Large area glossy ganoderma dish garden is cultivated, and Nutrient medium (bacterium bag) should be carried out in aseptic inoculation room.Because transfer room space is big, capacity is many, every day, inoculum concentration was up to up to ten thousand bacterium bags.And it is only suitable for the cultivation of little area in inoculating hood inoculation.Because of its day, inoculum concentration was low, time-consuming.Transfer room inoculation time is typically chosen around the Spring Festival.According to certain proportioning, raw material is mixed thoroughly by certain proportioning, be then charged in long 38cm width 17.5/18cm polyethylene bacterium bag.After normal-pressure sterilization 24 hours, move into transfer room and carry out artificial vaccination.
Nutrient medium includes that the raw material of following weight portion is made: wood flour 60 parts, corn cob 15 parts, Semen Maydis flour 10 parts, 12 parts of Testa Tritici, 1.2 parts of Gypsum Fibrosum, quick lime 1.2 parts, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 63% of Nutrient medium gross weight.
(3) send out bacterium: after inoculation, bacterium bag is moved to educate bacterium room added, put neat.Unglazed the educating of about 25 DEG C, bacterium room is sent out bacterium.
1. temperature control: send out bacterium early stage, educates bacterium indoor temperature at 22 DEG C-26 DEG C, and relative humidity is 50-60%, notes observing day and night temperature and educating bacterium room upper and lower temperature difference, and the temperature difference should not be excessive, tends to constant temperature optimal.Temperature is too high, too low or excessive temperature differentials all can suppress bacterium bag to grow and bacteria infection.
2. translation frame (smashing frame): constantly observe greenhouse temperature and hyphal development situation during sending out bacterium, smash buttress translation frame for about every ten days once, carefully reject the disease bag of living contaminants during translation frame and isolate, the timely destruction of serious pollution.When turning over, undercarriage to be gone up exchanges (because Yu Jun room lower floor temperature is low, mycelial growth is slow).After initial stage mycelium germination goes beyond bacterium bag shoulder, in good time with the bundle hole oxygen supplement at tying of Special sterilizing needle set, to promote mycelia robust growth.Within general 50 days, bacterium bag is sent out full.
3. ventilate oxygen supplement: at the noon every day calm period, open vent and within 1 hour, be aerated ventilating oxygen, to promote hyphal development.In educating bacterium in the case of proper temperature, if mycelial growth is slow, then prick hole oxygen supplement with special sterilization needle set.Prick hole and need to use professional, otherwise can large area infect.
4. lucifuge: educate bacterium room and be prone to hyphal development growth under conditions of unglazed, if sending out bacterium in the bacterium room of educating that luminosity is strong, can go out and buddingging in advance or mycelia catabiosis, affecting sesame quality.
(4) combination
1. after bacterium bag is sent out completely, bacterium bag is lowered the temperature 3-5 DEG C, broken bag combination again in 1-2 days.The temperature that bacterium is low is reduced, in sack inner heat after preventing resistance from closing by cooling.' hard waste ' can also be carried out by cooling, it is to avoid ' outer ripe interior life ' phenomenon, then, select window of opportunity to carry out large-scale cultivation base combination.
Carry out large-scale cultivation base combination: cultivation base best of breed period, the middle ten days and the last ten days in March, 16-26 DEG C Celsius of warmhouse booth diurnal temperature the most in good time, be preferably selected and be advisable in one week natural temperature little period that rises and falls.Combination too early, affects the time and quality buddingged because temperature low mycelia affinity crosses slowly；Too late, because temperature too high cultivation base miscellaneous bacteria infection rate is high, sesame rate is gone out low.
3. cultivation base combination operation code: the combination of super-huge glossy ganoderma dish garden cultivation base
Cultivation base bacterium bag.Selecting fabric width is long 330cm × wide 200cm polyethylene or polypropylene film bag, and the diameter after breadth more wide array is the biggest, and its capacity is the biggest.Ganoderma lucidum cultivation medium after combination is long 200cm × wide 50cm × high 45cm.
Environment purification, sterilization.1, cultivation base combination should be carried out in clean composed chamber.Before combination, reply composed chamber carries out disinfection, remove impurity bacterium of going out, and sprays wettability carbendazim class sterilizing about thing or gram mould clean aerosol fumigant is fumigated.2, featured little bacterium bag is entered composed chamber, spray little bacterium bag two ends sterilizing with gram mould clean diluent, it is possible to Burdick lamp sterilization is installed.
Elaborate manipulation.1, after washing one's hands with potassium permanganate diluent before staff enters the room, wear the working clothing sterilized, put on one's gloves, mask, footmuff, then enter workplace operation.2, staff is first with instruments such as Special sterilizing shears, cut off one tying of little bacterium bag, then bacterium bag is scratched downwards along clip sterilization cutter, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into egg size block (block greatest length is between 3-8cm) and puts in sack, put while be compacted (after combination the volume of cultivation base: volume sum=1.3 of bacterium rod before combination), rotate in time highly reaching 45cm and twist mouth and accused the combination of a cultivation base.3, points for attention: staff to examine the bacterium rod hyphal development situation after each peeling in anabolic process.As found with disease or doubtful disease, whole bacterium rod to isolate or to destroy, and must not put in sack, and otherwise, whole sack will be contaminated and break down.If it find that half-cooked bacterium is excellent, ' raw material ' all be rejected.Staff once touches disease carrying germ rod, row operation again after disinfecting in alcohol in time.
Wherein, use when loading sack and there are dismountable four walls and there is mould suitable for reading, in mould, spread thin film, thin film extends up to mould place suitable for reading, form described sack, bacterium rod is broken into bulk and puts in sack, and be inside compacted, when reaching preset height, after leveling upper surface, the surrounding of thin film is covered above, shed mould, prick at a glance every 10cm with pin cone at angle, base, to increase big bacterium bag bottom oxygen-supplying amount, promote that big bacterium bag upper and lower mycelia affinity is uniform.
Observation on Growth, the 7-10 days whole affinities of mycelia after combination, disease-free phenomenon, the big cultivation base entirety sesame that buddings is uniform, grows fine.
(5) management after combination.Manage the management in the sesame stage that is combined to budding for cultivation base；
1. temperature control: after cultivation base combination, in warmhouse booth, the temperature of optimum mycelia affinity is 20-26 DEG C；Therefore, should notice weather condition before combination, in should selecting one week, the weather fine warm natural temperature fluctuating little period is carried out.Temperature is too low or the metropolis that fluctuates affects mycelial growth affinity, increases miscellaneous bacteria infection rate.
2. light is controlled: scientific and reasonable promotes cultivation base bacteriumization quality and speed of buddingging with luminous energy.The happiness of Ganoderma habit own is cloudy and phototaxis is the strongest, so, cultivation base combination one week after planted agent reduces the light transmission capacity of straw mat, scattered light intensity of illumination 1500-2000LX, the 7-10 days whole affinities of mycelia, to adjust straw mat light transmission capacity (scattered light intensity of illumination 2500-3000LX) in time, promote cultivation base to budding speed.
3. oxygen is controlled: little bacterium rod is broken to be loaded in sack, and amount of oxygen own is abundant, now answers airtight greenhouse production booth vent 35 days, sesame will be gone out when buddingging after the whole affinity of cultivation base mycelia, at the noon every day calm period, open the ventilation oxygen supplement in 1 hour of partial ventilation mouth, promote that cultivation base is quickly buddingged；
4. prevention and control disease: the large-scale cultivation base after combination, due to air bump contact, there will be some defect phenomenon unavoidably.In order to avoid or reduce living contaminants phenomenon and occur, every three days of front first quarter moon is to medicinal liquid sterilizations such as canopy body, top, ground spray once visual Methylpartricin Sodium Laurylsulfates.
As being found to have yellow spotting phenomenon, cut out in time with Special sterilizing instrument except and affected part sterilized,.White mould contamination, destroy in time in this way.Penicillium pollution in this way, quarantines, and is not the most serious can also to go out sesame.
Large-scale live body grafting glossy ganoderma dish garden cultivation base group technology is that large-scale Ganoderma views and admires the potted landscape successful bottleneck of cultivation.Breaching this pass, the cultivation of large-scale glossy ganoderma dish garden just hits half.Therefore, large-scale glossy ganoderma dish garden large-scale cultivation base combination link is cultivated the most key.
Experimental example 1: Nutrient medium impact lignified on sporophore
Test group: use the Nutrient medium of embodiment 1: include that the raw material of following weight portion is made: wood flour 60 parts, corn cob 15 parts, Semen Maydis flour 10 parts, 12 parts of Testa Tritici, 1.2 parts of Gypsum Fibrosum, quick lime 1.2 parts, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 63% of Nutrient medium gross weight.
Contrast groups: use a patent 201310680939.2(live body glossy ganoderma dish garden manufacture method) in Nutrient medium: mass fraction is: weed tree sawdust 25%, wood particle 50%, wheat bran (i.e. Testa Tritici) 15%, Semen Maydis powder 5%, analysis for soybean powder 3%, Gypsum Fibrosum 1% and brown sugar 1%.
Two groups of cultivation of glossy ganoderma processes are same as in Example 1, but do not carry out " combination " operation, contrast with single Ganoderma sporophore after inoculation, a bacterium, growth.
Content of lignin measures: sporophore is pulverized after 60 DEG C of oven for drying, after crossing 40 mesh sieves, by 1: 10(W/V) add boiling water, in boiling water after being cooled to room temperature 25 DEG C: it is the sulphuric acid of 85% that the ratio of sulphuric acid=5:1 (v:v) adds mass fraction, it is sufficiently stirred for 4h under room temperature, adds 250mLdH2O and boil 1h, with drying the core pan sucking filtration to constant weight after being cooled to room temperature, occur without when react with 10%BaCl2 to washing liquid with 60 DEG C of hot dH2O washing deep, weigh after core pan drying of disturbing by making noise.Two groups take 3 strain Ganoderma sporophories respectively and are measured, and content is by being calculated as follows content of lignin:
Lignin (%)=[(funnel weight before funnel weight-sucking filtration after sucking filtration)/sample] × 100%
Result: test group content of lignin: 18.1%, 17.9%, 16.8%, average is 17.6%；
Contrast groups content of lignin: 12.7%, 13.4%, 14.5%, average is 13.5%.
Conclusion: relative to contrast groups, the bacterial strain color after the strain growth that experimental group is cultivated is beautiful, sheet shape is mellow and full, make sporophore degree of lignification high, and hardness is big.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that: these embodiments can carry out in the case of without departing from the principle of the present invention and objective multiple change, revise, replace and modification, the scope of the present invention is limited by claim and equivalent thereof.
Claims (10)
1. one kind large-scale live body glossy ganoderma dish garden cultivation base combination and management technique, it is characterised in that comprise the following steps:
(1) strain is selected；
(2) inoculation: with selected strain to through sterilizing, load Nutrient medium bacterium bag inoculate；
(3) bacterium is sent out: carry out postvaccinal bacterium bag sending out bacterium；
(4) combination: after bacterium bag is sent out completely, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into bulk and puts in sack, inside it is compacted, when reaching preset height, after leveling upper surface, sack sealing has i.e. been accused the combination of a cultivation base, after every bag of combination, if prick dry hole at angle, base；Combination period warmhouse booth diurnal temperature 16-26 DEG C；
(5) management after combination；
1. temperature control: after cultivation base combination, in warmhouse booth, the temperature of optimum mycelia affinity is 20-26 DEG C；Therefore, should notice weather condition before combination, in should selecting one week, the weather fine warm natural temperature fluctuating little period is carried out；
2. control light: cultivation base combination one week after planted agent reduces the light transmission capacity of straw mat, scattered light intensity of illumination 1500-2000Lx, the 7-10 days whole affinities of mycelia, will adjust straw mat light transmission capacity, scattered light intensity of illumination 2500-3000Lx in time, promote cultivation base to budding speed；
3. oxygen is controlled: little bacterium rod is broken to be loaded in sack, and amount of oxygen own is abundant, now answers airtight greenhouse production booth vent 35 days, sesame will be gone out when buddingging after the whole affinity of cultivation base mycelia, at the noon every day calm period, open the ventilation oxygen supplement in 1 hour of partial ventilation mouth, promote that cultivation base is quickly buddingged.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterized in that, use the mould with dismountable four walls when described step (4) combination loads sack, in mould, spread thin film, thin film extends up to mould place suitable for reading, form described sack, bacterium rod is broken into bulk and puts in sack, and be inside compacted, when reaching preset height, after leveling upper surface, the surrounding of thin film is covered above, shed mould, if dry hole is pricked at angle, base.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 2 combination and management technique, it is characterised in that in described step (4), the Ganoderma lucidum cultivation medium after combination is long 200-300cm × wide 50-60cm × high 45cm；Thin film is long 330cm × wide 200cm.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 and 2 combination and management technique, it is characterised in that described step (4) bacterium bag send out full after also include the step of cooling, will send out full after bacterium bag lower the temperature 3-5 DEG C, broken bag combination again in 1-2 days.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterised in that described step (4) combination is carried out in composed chamber, before combination, reply composed chamber carries out disinfection, remove impurity bacterium of going out；Bacterium bag is entered composed chamber, sprays little bacterium bag two ends sterilizing with gram mould clean diluent, or Burdick lamp sterilization is installed.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterized in that, after during described step (4) combination, staff washes one's hands with potassium permanganate diluent before entering the room, wear the working clothing sterilized, put on one's gloves, mask, footmuff, then enter composed chamber operation；First one tying of bacterium bag is cut off with sterilization shears, then bacterium bag is scratched downwards along clip sterilization cutter, peel off bacterium bag hides, the bacterium rod peelling off skin is broken into egg size bulk and puts in sack, put while be compacted, in time highly reaching preset height, sack sealing has been accused the combination of a cultivation base；To examine the bacterium rod hyphal development situation after each peeling in anabolic process, as found with disease or doubtful disease, whole bacterium rod to isolate or to destroy, and must not put in sack；If it find that half-cooked bacterium is excellent, half-cooked bacterium rod all be rejected；Staff once touches disease carrying germ rod, row operation again after disinfecting in alcohol in time.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterized in that, described step (2) Middle nutrition base includes that the raw material of following weight portion is made: wood flour 60-70 part, corn cob 15-25 part, Semen Maydis flour 10-12 part, Testa Tritici 10-12 part, Gypsum Fibrosum 1-1.5 part, quick lime 1-1.2 part, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 60-65% of Nutrient medium gross weight.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 5 combination and management technique, it is characterized in that, described step (2) Middle nutrition base includes that the raw material of following weight portion is made: wood flour 60 parts, corn cob 15 parts, Semen Maydis flour 10 parts, 12 parts of Testa Tritici, 1.2 parts of Gypsum Fibrosum, quick lime 1.2 parts, potassium dihydrogen phosphate 0.3 part, 0.3 part of magnesium sulfate, water；Raw material is added water by proportioning and mixes thoroughly, mix rear water content thoroughly and account for the 60-65% of Nutrient medium gross weight.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterised in that send out bacterium in described step (3): after inoculation, move bacterium bag that to educate bacterium room added, puts neatly；
1. temperature control: send out bacterium early stage, educates bacterium indoor temperature at 22 DEG C-26 DEG C, and relative humidity is 50-60%, notes observing the temperature difference and educating bacterium room upper and lower temperature difference, and the temperature difference should not be excessive；
2. translation frame: carried out translation frame every 8-12 days, when turning over, undercarriage to be gone up exchanges, and during translation frame, rejects dysplasia and disease bag in time, reduces polluter；
3. ventilate oxygen supplement；Educate bacterium indoor and answer timing ventilation ventilating oxygen；Educate bacterium indoor temperature suitable in the case of, if mycelial growth is slow, then prick hole oxygen supplement with sterilization needle set；
4. lucifuge: educate bacterium room and be prone to hyphal development growth under conditions of unglazed.
Large-scale live body glossy ganoderma dish garden cultivation base the most according to claim 1 combination and management technique, it is characterised in that management is combined to budding for cultivation base the management in sesame stage in described step (5)；
1. temperature control: after cultivation base combination, in warmhouse booth, the temperature of optimum mycelia affinity is 20-26 DEG C；
2. control light: cultivation base combination one week after inscattering light intensity of illumination 1500-2000Lx, after the 7-10 days whole affinities of mycelia, scattered light intensity of illumination 2500-3000Lx will be adjusted in time, promote cultivation base to budding speed；
3. oxygen is controlled: little bacterium rod is broken to be loaded in sack, amount of oxygen own is abundant, now should airtight greenhouse production booth vent 35 days, sesame will be gone out after the whole affinity of cultivation base mycelia when buddingging, every day, partial ventilation mouth ventilation oxygen supplement was opened in timing, promoted that cultivation base is quickly buddingged；
4. prevention and control disease: the large-scale cultivation base after combination, canopy body, top, medicinal liquid of ground spray are sterilized by front first quarter moon for every three days；As found, cultivation base has yellow spotting phenomenon, cuts out in time with disinfection tool except and sterilizes affected part；White mould contamination, destroy in time in this way；Penicillium pollution in this way, quarantines, and the most serious can also go out sesame.
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