CN104254776B - 提取和储存核酸的方法和组合物 - Google Patents

提取和储存核酸的方法和组合物 Download PDF

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Publication number
CN104254776B
CN104254776B CN201380022584.6A CN201380022584A CN104254776B CN 104254776 B CN104254776 B CN 104254776B CN 201380022584 A CN201380022584 A CN 201380022584A CN 104254776 B CN104254776 B CN 104254776B
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solid
rna
solid matrix
nucleic acids
cellulose
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Chinese (zh)
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CN104254776A (zh
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李兵
D.R.穆尔
E.L.克瓦姆
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GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
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General Electric Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380022584.6A 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物 Active CN104254776B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460076 2012-04-30
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CN104254776A CN104254776A (zh) 2014-12-31
CN104254776B true CN104254776B (zh) 2016-09-28

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CN201380022584.6A Active CN104254776B (zh) 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物

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Country Link
US (1) US9044738B2 (enExample)
EP (1) EP2844755B1 (enExample)
JP (3) JP6248095B2 (enExample)
CN (1) CN104254776B (enExample)
CA (1) CA2870038C (enExample)
ES (1) ES2609763T3 (enExample)
IN (1) IN2014DN08223A (enExample)
WO (1) WO2013165870A1 (enExample)

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EP3528950A4 (en) * 2016-10-24 2020-05-27 Biomatrica, INC. STABILIZATION OF NUCLEIC ACIDS ON PAPER
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CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
CN113227795B (zh) * 2019-01-25 2024-09-03 积水化学工业株式会社 核酸的分离方法、核酸的分离试剂盒和检测芯片
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Also Published As

Publication number Publication date
EP2844755B1 (en) 2016-11-16
US9044738B2 (en) 2015-06-02
WO2013165870A1 (en) 2013-11-07
JP2018068303A (ja) 2018-05-10
JP6248095B2 (ja) 2017-12-13
CA2870038A1 (en) 2013-11-07
ES2609763T3 (es) 2017-04-24
EP2844755A1 (en) 2015-03-11
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
IN2014DN08223A (enExample) 2015-05-15
US20130289265A1 (en) 2013-10-31
JP2015518569A (ja) 2015-07-02
CA2870038C (en) 2021-08-03
EP2844755A4 (en) 2015-03-11
JP6847365B2 (ja) 2021-03-24

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Effective date of registration: 20210906

Address after: England

Patentee after: Global life science solutions UK Ltd.

Address before: Buckinghamshire

Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20210906

Address after: Buckinghamshire

Patentee after: GE HEALTHCARE UK Ltd.

Address before: New York State, USA

Patentee before: General Electric Co.