SG11201806794RA - Purification of rna fractions using a hydrophilic polymeric material - Google Patents

Purification of rna fractions using a hydrophilic polymeric material

Info

Publication number
SG11201806794RA
SG11201806794RA SG11201806794RA SG11201806794RA SG11201806794RA SG 11201806794R A SG11201806794R A SG 11201806794RA SG 11201806794R A SG11201806794R A SG 11201806794RA SG 11201806794R A SG11201806794R A SG 11201806794RA SG 11201806794R A SG11201806794R A SG 11201806794RA
Authority
SG
Singapore
Prior art keywords
international
present
relates
nucleotides
pct
Prior art date
Application number
SG11201806794RA
Inventor
Thomas Laufer
Markus Beier
Mustafa Kahraman
Andreas Keller
Original Assignee
Hummingbird Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hummingbird Diagnostics Gmbh filed Critical Hummingbird Diagnostics Gmbh
Publication of SG11201806794RA publication Critical patent/SG11201806794RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/34—Purifying; Cleaning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 21 September 2017 (21.09.2017) WIPO I PCT (10) International Publication Number WO 2017/157650 Al 111111111111110111011111111111010111110 0111111111111111111111111111111111111110111111 (51) International Patent Classification: G01N 1/34 (2006.01) GO1N 1/40 (2006.01) (21) International Application Number: PCT/EP2017/054582 (22) International Filing Date: 28 February 2017 (28.02.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 16161077.9 18 March 2016 (18.03.2016) EP (71) Applicant: HUMMINGBIRD DIAGNOSTICS GMBH [DE/DE]; Im Neuenheimer Feld 583, 69120 Heidelberg (DE). (72) Inventors: LAUFER, Thomas; Lessingstr. 17, 68535 Edingen-Neckarhausen (DE). BEIER, Markus; Marc- Aurel-Weg 20, 69469 Weinheim (DE). KAHRAMAN, Mustafa; Waldhausweg 7, 66123 Saarbriicken (DE). KELLER, Andreas; Albert-Schweitzer-Str. 6, 66346 Piit- tlingen (DE). (74) Agent: GEEING, Andrea; ZSP Patentanwalte PartG mbB, Hansastrasse 32, 80686 Miinchen (DE). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) W O 20 17157 / 65 0 Al (54) Title: PURIFICATION OF RNA FRACTIONS USING A HYDROPHILIC POLYMERIC MATERIAL (57) : The present invention relates to a method of removing an RNA fraction with > 200 nucleotides in length from a whole blood sample. The present invention also relates to a method of purifying an RNA fraction with < 200 nucleotides in length from a whole blood sample. The present invention further relates to a method of determining the level of RNA molecules with < 200 nucleotides in length. In addition, the present invention relates to a method for diagnosing a disease in an individual. Moreover, the present invention relates to a kit which is useful for carrying out the methods of the present invention.
SG11201806794RA 2016-03-18 2017-02-28 Purification of rna fractions using a hydrophilic polymeric material SG11201806794RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16161077 2016-03-18
PCT/EP2017/054582 WO2017157650A1 (en) 2016-03-18 2017-02-28 Purification of rna fractions using a hydrophilic polymeric material

Publications (1)

Publication Number Publication Date
SG11201806794RA true SG11201806794RA (en) 2018-09-27

Family

ID=55587144

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201806794RA SG11201806794RA (en) 2016-03-18 2017-02-28 Purification of rna fractions using a hydrophilic polymeric material
SG10202010005TA SG10202010005TA (en) 2016-03-18 2017-02-28 Purification of rna fractions using a hydrophilic polymeric material

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202010005TA SG10202010005TA (en) 2016-03-18 2017-02-28 Purification of rna fractions using a hydrophilic polymeric material

Country Status (11)

Country Link
US (1) US11566290B2 (en)
EP (1) EP3430373B1 (en)
JP (1) JP7093305B2 (en)
CN (1) CN109154544B (en)
AU (1) AU2017233436B2 (en)
DK (1) DK3430373T3 (en)
ES (1) ES2952950T3 (en)
FI (1) FI3430373T3 (en)
PL (1) PL3430373T3 (en)
SG (2) SG11201806794RA (en)
WO (1) WO2017157650A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10656059B2 (en) 2018-03-07 2020-05-19 Alcala Pharmaceutical, Inc. Method for qualitative and quantitative multiplexing of drug analytes from biological samples
US11162921B2 (en) 2019-04-01 2021-11-02 Alcala Pharmaceutical, Inc. Method for extracting pharmacogenetic DNA from biological fluid retained in a solid resin collection device
GB2623570A (en) * 2022-10-21 2024-04-24 Wobble Genomics Ltd Method and products for biomarker identification

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
DE102005059315A1 (en) 2005-12-09 2007-06-14 Qiagen Gmbh Process for the enrichment of short-chain nucleic acids
EP2407540A1 (en) 2010-07-15 2012-01-18 Qiagen GmbH Method for purifying a target nucleic acid
WO2013022342A1 (en) 2011-08-09 2013-02-14 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg A method of analysing a blood sample of a subject for the presence of a foetal disease or condition marker
WO2013043562A1 (en) 2011-09-23 2013-03-28 Waters Technologies Corporation Solid phase extraction device for dried sample cards
US20130116597A1 (en) 2011-11-04 2013-05-09 Phenomenex, Inc. Method and apparatus for acquiring blood for testing
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
WO2014143714A2 (en) * 2013-03-15 2014-09-18 Ge Healthcare Uk Limited Methods for one step nucleic acid amplification of non-eluted samples
ES2705574T3 (en) 2013-10-22 2019-03-26 Hoffmann La Roche Procedures for measuring cell-free virus particles from drops of dried blood

Also Published As

Publication number Publication date
AU2017233436A1 (en) 2018-10-11
EP3430373A1 (en) 2019-01-23
WO2017157650A1 (en) 2017-09-21
JP7093305B2 (en) 2022-06-29
JP2019513352A (en) 2019-05-30
US20200199672A1 (en) 2020-06-25
AU2017233436B2 (en) 2022-11-24
EP3430373B1 (en) 2023-07-05
US11566290B2 (en) 2023-01-31
DK3430373T3 (en) 2023-10-02
CN109154544A (en) 2019-01-04
PL3430373T3 (en) 2023-10-30
CN109154544B (en) 2024-05-14
FI3430373T3 (en) 2023-08-18
ES2952950T3 (en) 2023-11-07
SG10202010005TA (en) 2020-11-27

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