WO2013165870A1 - Methods and compositions for extraction and storage of nucleic acids - Google Patents

Methods and compositions for extraction and storage of nucleic acids Download PDF

Info

Publication number
WO2013165870A1
WO2013165870A1 PCT/US2013/038576 US2013038576W WO2013165870A1 WO 2013165870 A1 WO2013165870 A1 WO 2013165870A1 US 2013038576 W US2013038576 W US 2013038576W WO 2013165870 A1 WO2013165870 A1 WO 2013165870A1
Authority
WO
WIPO (PCT)
Prior art keywords
solid matrix
nucleic acids
sample
rna
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/038576
Other languages
English (en)
French (fr)
Inventor
Bing Li
David Roger Moore
Erik Leeming KVAM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Priority to EP13784927.9A priority Critical patent/EP2844755B1/en
Priority to IN8223DEN2014 priority patent/IN2014DN08223A/en
Priority to CN201380022584.6A priority patent/CN104254776B/zh
Priority to JP2015510354A priority patent/JP6248095B2/ja
Priority to ES13784927.9T priority patent/ES2609763T3/es
Priority to CA2870038A priority patent/CA2870038C/en
Publication of WO2013165870A1 publication Critical patent/WO2013165870A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

Definitions

  • the present disclosure generally relates to solid compositions for ambient extraction, stabilization, and preservation of nucleic acids from a biological sample in a dry format. Methods for extracting, collecting, preserving, and recovering nucleic acids from the solid compositions are also described.
  • a composition comprising a protein denaturant, a reducing agent, and a buffer is present in the dry solid matrix of this disclosure.
  • the composition may comprise one or more of each of the above-listed components.
  • the composition may optionally further comprise an ultraviolet (UV) inhibitor, a free-radical trap, an RNase inhibitor, a chelator, or any combination thereof.
  • UV ultraviolet
  • the skilled artisan will appreciate that numerous protein denaturants are known in the art and can be empirically selected for use in the compositions and methods described here.
  • X is an anion, which is a radical containing one or more formal negative charge(s).
  • Buffer as used herein includes, for example, 2-Amino-2- hydroxymethyl-propane-l,3-diol (Tris), 2-(N-morpholino)ethanesulfonic acid (MES), 3- (N-morpholino)propanesulfonic acid (MOPS), citrate buffers, 4-(2-hydroxyethyl)-l- piperazineethanesulfonic acid (HEPES), and phosphate buffers.
  • This list of potential buffers is for illustrative purposes only. The skilled artisan would recognize that the pH of the buffer selected for use in the compositions and methods disclosed herein is relevant. The pH of the buffer will typically be in the range of 3 to 8.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/US2013/038576 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids Ceased WO2013165870A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP13784927.9A EP2844755B1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids
IN8223DEN2014 IN2014DN08223A (enExample) 2012-04-30 2013-04-29
CN201380022584.6A CN104254776B (zh) 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物
JP2015510354A JP6248095B2 (ja) 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物
ES13784927.9T ES2609763T3 (es) 2012-04-30 2013-04-29 Métodos y composiciones para la extracción y el almacenamiento de ácidos nucleicos
CA2870038A CA2870038C (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/460,076 2012-04-30
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids

Publications (1)

Publication Number Publication Date
WO2013165870A1 true WO2013165870A1 (en) 2013-11-07

Family

ID=49477845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/038576 Ceased WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Country Status (8)

Country Link
US (1) US9044738B2 (enExample)
EP (1) EP2844755B1 (enExample)
JP (3) JP6248095B2 (enExample)
CN (1) CN104254776B (enExample)
CA (1) CA2870038C (enExample)
ES (1) ES2609763T3 (enExample)
IN (1) IN2014DN08223A (enExample)
WO (1) WO2013165870A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
CN107076764A (zh) * 2014-11-20 2017-08-18 通用电气医疗集团英国有限公司 检测稳定在固体载体材料上的痴呆和阿尔茨海默病相关生物标记
WO2017157650A1 (en) * 2016-03-18 2017-09-21 Hummingbird Diagnostics Gmbh Purification of rna fractions using a hydrophilic polymeric material
WO2021229066A1 (en) * 2020-05-14 2021-11-18 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
US11266337B2 (en) 2015-09-09 2022-03-08 Drawbridge Health, Inc. Systems, methods, and devices for sample collection, stabilization and preservation
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US10829291B2 (en) * 2012-06-20 2020-11-10 Thermo Fischer Scientific Baltics UAB Method to prevent silica-based column aging
AU2014307875B2 (en) * 2013-08-16 2020-07-02 Global Life Sciences Solutions Operations UK Ltd Methods and compositions for extraction and storage of nucleic acids
EP3942931A1 (en) 2014-06-10 2022-01-26 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
GB201505516D0 (en) * 2015-03-31 2015-05-13 Ge Healthcare Uk Ltd Improvements in and relating to biological sample collectors and handling thereof
US10000742B2 (en) * 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
EP4242628B1 (en) 2015-12-08 2025-07-02 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
US11166658B2 (en) * 2016-07-28 2021-11-09 Invitae Corporation Blood sampling system and method
WO2018081161A1 (en) * 2016-10-24 2018-05-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
GB2590814B (en) 2017-01-10 2021-11-03 Drawbridge Health Inc Devices, systems, and methods for sample collection
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
SG11202104814XA (en) * 2019-01-25 2021-08-30 Sekisui Chemical Co Ltd Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
GB202211645D0 (en) 2022-08-09 2022-09-21 Hemodx As Kit
SE2351278A1 (en) * 2023-11-08 2025-05-09 Sequrna Ab Dry storage

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20110081363A1 (en) * 2009-05-11 2011-04-07 Biomatrica, Inc. Compositions and methods for biological sample storage
US8088576B2 (en) * 2004-04-30 2012-01-03 Siemens Aktiengesellschaft Method and assembly for DNA isolation with dry reagents

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4540506A (en) 1983-04-15 1985-09-10 Genex Corporation Composition for cleaning drains clogged with deposits containing hair
CA1255197A (en) * 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
US5173422A (en) 1986-08-22 1992-12-22 Miles Inc. Monoclonal antibodies specific for human glycoalbumin
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
US5756126A (en) 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
WO1996039813A1 (en) 1995-06-07 1996-12-19 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
JPH11510393A (ja) 1995-08-09 1999-09-14 インスティチュート ナショナル ドゥ ラ セント エト ドゥ ラ リサーチェ メディカル 白血病マーカーとしておよび乳癌の予後において有用な単離された核酸分子
US5939259A (en) 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US20010039010A1 (en) 1998-09-03 2001-11-08 Leigh Alexander Burgoyne Sample collection medium incorporating material for sample visualization
WO2000066605A2 (en) 1999-04-30 2000-11-09 Cyclops Genome Sciences Limited Polynucleotides
AU4353500A (en) 1999-04-30 2000-11-17 Whatman, Inc. Substrate including anionic detergent for purifying nucleic acid
EP1220944B1 (en) 1999-09-24 2010-09-15 Ambion, Inc. Nuclease inhibitor cocktail
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US7531362B2 (en) 2001-06-07 2009-05-12 Medmira Inc. Rapid diagnostic assay
WO2003020924A2 (en) 2001-09-05 2003-03-13 Whatman Plc Stable storage of proteins
GB0129816D0 (en) * 2001-12-13 2002-01-30 The Technology Partnership Plc Testing device for chemical or biochemical analysis
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
EP1552005A4 (en) 2002-07-10 2010-12-15 Massachusetts Inst Technology DEVICE AND METHOD FOR ISOLATING A NUCLEIC ACID FROM A SAMPLE
DE10231659B4 (de) 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
US7267950B2 (en) * 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
JP2007502218A (ja) * 2003-05-23 2007-02-08 ユィロス・パテント・アクチボラグ 親水性／疎水性表面
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
ATE514791T1 (de) 2003-07-09 2011-07-15 Genvault Corp Raumtemperatur-eluierung der nukleinsäuren
JP3714940B2 (ja) 2003-11-07 2005-11-09 株式会社日立ハイテクノロジーズ Ｒｎａ抽出方法、および生体材料の分析方法
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
BRPI0509693A (pt) 2004-04-09 2007-10-09 Res Think Tank Inc dispositivo e método para coletar, armazenar ou transportar um espécime biológico, método para recuperar um espécime biológico, e, kit para coletar, armazenar e transportar um espécime biológico
WO2005116081A2 (en) * 2004-05-24 2005-12-08 Genvault Corporation Stable protein storage and stable nucleic acid storage in recoverable form
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
US20090208919A1 (en) 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
US8158357B2 (en) 2005-03-16 2012-04-17 Dna Genotek Inc. Compositions and method for storage of nucleic acid from bodily fluids
GB0505909D0 (en) 2005-03-23 2005-04-27 Univ Leeds Formulations
US20070015165A1 (en) 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
EP2384432B1 (en) * 2007-06-21 2016-12-28 Gen-Probe Incorporated Instrument and receptacles for performing processes
US20090053704A1 (en) 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
WO2009070558A1 (en) * 2007-11-30 2009-06-04 Ge Healthcare Bio-Sciences Corp. Method for isolation of genomic dna, rna and proteins from a single sample
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US20100209957A1 (en) 2008-06-20 2010-08-19 Genvault Corporation Biosample storage devices and methods of use thereof
EP2334792B1 (en) 2008-09-12 2020-09-09 GenTegra LLC Matrices and media for storage and stabilization of biomolecules
EP2412020B1 (en) * 2009-03-24 2020-09-30 University Of Chicago Slip chip device and methods
AU2011244348B2 (en) 2010-04-20 2015-02-12 Octapharma Ag New stabilizing agent for pharmaceutical proteins
EP2388312A1 (en) 2010-05-17 2011-11-23 Curetis AG Universally applicable lysis buffer and processing methods for the lysis of bodily samples
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012075471A1 (en) 2010-12-04 2012-06-07 Hui-Yu Liu Rna stability enhancer
US20120152743A1 (en) 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples
GB201103257D0 (en) 2011-02-25 2011-04-13 Ge Healthcare Uk Ltd Paper support and method of recovering biological material therefrom
GB2496122A (en) 2011-10-31 2013-05-08 Ge Healthcare Bio Sciences Ab Biological sample preservation on paper
CN103173432B (zh) * 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US8088576B2 (en) * 2004-04-30 2012-01-03 Siemens Aktiengesellschaft Method and assembly for DNA isolation with dry reagents
US20110081363A1 (en) * 2009-05-11 2011-04-07 Biomatrica, Inc. Compositions and methods for biological sample storage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2844755A4 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107076764A (zh) * 2014-11-20 2017-08-18 通用电气医疗集团英国有限公司 检测稳定在固体载体材料上的痴呆和阿尔茨海默病相关生物标记
EP3221704A4 (en) * 2014-11-20 2018-04-11 GE Healthcare UK Limited Detecting dementia and alzheimer's disease associated biomarkers stabilized on solid support materials
US11266337B2 (en) 2015-09-09 2022-03-08 Drawbridge Health, Inc. Systems, methods, and devices for sample collection, stabilization and preservation
WO2017157650A1 (en) * 2016-03-18 2017-09-21 Hummingbird Diagnostics Gmbh Purification of rna fractions using a hydrophilic polymeric material
AU2017233436B2 (en) * 2016-03-18 2022-11-24 Hummingbird Diagnostics Gmbh Purification of RNA fractions using a hydrophilic polymeric material
US11566290B2 (en) 2016-03-18 2023-01-31 Hummingbird Diagnostics Gmbh Purification of RNA fractions using a hydrophilic polymeric material
CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
WO2021229066A1 (en) * 2020-05-14 2021-11-18 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method

Also Published As

Publication number Publication date
CN104254776A (zh) 2014-12-31
EP2844755A4 (en) 2015-03-11
US9044738B2 (en) 2015-06-02
EP2844755B1 (en) 2016-11-16
IN2014DN08223A (enExample) 2015-05-15
CA2870038A1 (en) 2013-11-07
JP2020092709A (ja) 2020-06-18
US20130289265A1 (en) 2013-10-31
CN104254776B (zh) 2016-09-28
CA2870038C (en) 2021-08-03
JP6248095B2 (ja) 2017-12-13
EP2844755A1 (en) 2015-03-11
JP2015518569A (ja) 2015-07-02
JP6847365B2 (ja) 2021-03-24
ES2609763T3 (es) 2017-04-24
JP2018068303A (ja) 2018-05-10

Similar Documents

Publication Publication Date Title
US9044738B2 (en) Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) Formulations for nucleic acid stabilization on solid substrates
EP2935583B1 (en) Formulations for nucleic acid stabilization on solid substrates
US9040679B2 (en) Methods and compositions for extraction and storage of nucleic acids
CA2921286C (en) Methods and compositions for extraction and storage of nucleic acids
WO2011104027A1 (en) Process for parallel isolation and/or purification of rna and dna
CN103370412B (zh) 核糖核酸稳定性增强剂
Van Eijsden et al. A universal fixation method based on quaternary ammonium salts (RNA later) for omics-technologies: Saccharomyces cerevisiae as a case study
US9534214B2 (en) Substrates and associated methods for elution of nucleic acids
US9719082B2 (en) Substrates and associated methods for elution of nucleic acids
EP3024919A1 (en) Method for nuclei storage
CN119020463A (zh) 一种动物毛发样本基因组dna文库的构建方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13784927

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2870038

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2015510354

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2013784927

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2013784927

Country of ref document: EP