CA2870038C - Methods and compositions for extraction and storage of nucleic acids - Google Patents

Methods and compositions for extraction and storage of nucleic acids Download PDF

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Publication number
CA2870038C
CA2870038C CA2870038A CA2870038A CA2870038C CA 2870038 C CA2870038 C CA 2870038C CA 2870038 A CA2870038 A CA 2870038A CA 2870038 A CA2870038 A CA 2870038A CA 2870038 C CA2870038 C CA 2870038C
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Canada
Prior art keywords
solid matrix
nucleic acids
rna
sample
matrix
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Active
Application number
CA2870038A
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English (en)
French (fr)
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CA2870038A1 (en
Inventor
Bing Li
David Roger Moore
Erik Leeming Kvam
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Global Life Sciences Solutions Operations UK Ltd
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Global Life Sciences Solutions Operations UK Ltd
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Publication of CA2870038A1 publication Critical patent/CA2870038A1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2870038A 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids Active CA2870038C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
US13/460,076 2012-04-30
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CA2870038A1 CA2870038A1 (en) 2013-11-07
CA2870038C true CA2870038C (en) 2021-08-03

Family

ID=49477845

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2870038A Active CA2870038C (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Country Status (8)

Country Link
US (1) US9044738B2 (enExample)
EP (1) EP2844755B1 (enExample)
JP (3) JP6248095B2 (enExample)
CN (1) CN104254776B (enExample)
CA (1) CA2870038C (enExample)
ES (1) ES2609763T3 (enExample)
IN (1) IN2014DN08223A (enExample)
WO (1) WO2013165870A1 (enExample)

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US20230303995A1 (en) * 2020-05-14 2023-09-28 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
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Also Published As

Publication number Publication date
EP2844755A1 (en) 2015-03-11
EP2844755B1 (en) 2016-11-16
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
JP6847365B2 (ja) 2021-03-24
EP2844755A4 (en) 2015-03-11
US9044738B2 (en) 2015-06-02
JP2015518569A (ja) 2015-07-02
CN104254776B (zh) 2016-09-28
WO2013165870A1 (en) 2013-11-07
CA2870038A1 (en) 2013-11-07
US20130289265A1 (en) 2013-10-31
JP2018068303A (ja) 2018-05-10
ES2609763T3 (es) 2017-04-24
IN2014DN08223A (enExample) 2015-05-15
JP6248095B2 (ja) 2017-12-13

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