JP6248095B2 - 核酸の抽出及び貯蔵のための方法及び組成物 - Google Patents

核酸の抽出及び貯蔵のための方法及び組成物 Download PDF

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JP6248095B2
JP6248095B2 JP2015510354A JP2015510354A JP6248095B2 JP 6248095 B2 JP6248095 B2 JP 6248095B2 JP 2015510354 A JP2015510354 A JP 2015510354A JP 2015510354 A JP2015510354 A JP 2015510354A JP 6248095 B2 JP6248095 B2 JP 6248095B2
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solid matrix
rna
nucleic acid
sample
acid
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JP2015518569A5 (enExample
JP2015518569A (ja
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リ，ビン
ムーア，デイヴィッド・ロジャー
クヴァム，エリック・リーミング
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General Electric Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015510354A 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物 Active JP6248095B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460,076 2012-04-30
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Related Child Applications (1)

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JP2017225326A Division JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物

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JP2015518569A JP2015518569A (ja) 2015-07-02
JP2015518569A5 JP2015518569A5 (enExample) 2016-06-09
JP6248095B2 true JP6248095B2 (ja) 2017-12-13

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JP2015510354A Active JP6248095B2 (ja) 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物
JP2017225326A Active JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物
JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

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JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

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US (1) US9044738B2 (enExample)
EP (1) EP2844755B1 (enExample)
JP (3) JP6248095B2 (enExample)
CN (1) CN104254776B (enExample)
CA (1) CA2870038C (enExample)
ES (1) ES2609763T3 (enExample)
IN (1) IN2014DN08223A (enExample)
WO (1) WO2013165870A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US10829291B2 (en) * 2012-06-20 2020-11-10 Thermo Fischer Scientific Baltics UAB Method to prevent silica-based column aging
EP3942931A1 (en) 2014-06-10 2022-01-26 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
US20180031575A1 (en) * 2014-11-20 2018-02-01 Ge Healthcare Uk Limited Detecting Dementia and Alzheimer's Disease Associated Biomarkers Stabilized on Solid Support Materials
GB201505516D0 (en) * 2015-03-31 2015-05-13 Ge Healthcare Uk Ltd Improvements in and relating to biological sample collectors and handling thereof
ES2912965T3 (es) 2015-09-09 2022-05-30 Drawbridge Health Inc Dispositivos para la recopilación, estabilización y conservación de muestras
US10000742B2 (en) * 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
KR20250047404A (ko) 2015-12-08 2025-04-03 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
SG11201806794RA (en) * 2016-03-18 2018-09-27 Hummingbird Diagnostics Gmbh Purification of rna fractions using a hydrophilic polymeric material
CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
US11166658B2 (en) * 2016-07-28 2021-11-09 Invitae Corporation Blood sampling system and method
EP3528950A4 (en) * 2016-10-24 2020-05-27 Biomatrica, INC. STABILIZATION OF NUCLEIC ACIDS ON PAPER
CN210383905U (zh) 2017-01-10 2020-04-24 集联健康有限公司 一种用于从受试者收集流体样品的装置以及运输套筒
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
CN113227795B (zh) * 2019-01-25 2024-09-03 积水化学工业株式会社 核酸的分离方法、核酸的分离试剂盒和检测芯片
KR20230012003A (ko) * 2020-05-14 2023-01-25 바이오에코 라이프 사이언시스 게엠베하 상승된 온도에서의 핵산의 단리
US20230332132A1 (en) * 2020-08-28 2023-10-19 Kao Corporation Rna storage method
GB202211645D0 (en) 2022-08-09 2022-09-21 Hemodx As Kit
SE2351278A1 (en) * 2023-11-08 2025-05-09 Sequrna Ab Dry storage

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4540506A (en) 1983-04-15 1985-09-10 Genex Corporation Composition for cleaning drains clogged with deposits containing hair
CA1255197A (en) * 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
US5173422A (en) 1986-08-22 1992-12-22 Miles Inc. Monoclonal antibodies specific for human glycoalbumin
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
US5756126A (en) 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
DE96915910T1 (de) 1995-06-07 2004-04-22 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
EP0854923A2 (en) 1995-08-09 1998-07-29 Institut National De La Sante Et De La Recherche Medicale (Inserm) Isolated nucleic acid molecules useful as leukemia markers and in breast cancer prognosis
US5939259A (en) 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US20010039010A1 (en) 1998-09-03 2001-11-08 Leigh Alexander Burgoyne Sample collection medium incorporating material for sample visualization
EP1175431A4 (en) 1999-04-30 2002-09-11 Whatman Inc SUBSTRATE CONTAINING ANIONIC NUCLEIC ACID PURIFICATION DETERGENT
ATE347616T1 (de) 1999-04-30 2006-12-15 Cyclops Genome Sciences Ltd Ribonukleinsäurederivate
WO2001021830A1 (en) 1999-09-24 2001-03-29 Ambion, Inc. Nuclease inhibitor cocktail
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US7531362B2 (en) 2001-06-07 2009-05-12 Medmira Inc. Rapid diagnostic assay
EP1423514A2 (en) 2001-09-05 2004-06-02 WHATMAN plc Stable storage of proteins
GB0129816D0 (en) * 2001-12-13 2002-01-30 The Technology Partnership Plc Testing device for chemical or biochemical analysis
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040126783A1 (en) 2002-07-10 2004-07-01 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
DE10231659B4 (de) 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
US7267950B2 (en) 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
EP1628905A1 (en) * 2003-05-23 2006-03-01 Gyros Patent Ab Hydrophilic/hydrophobic surfaces
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
EP1641944B1 (en) 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
JP3714940B2 (ja) 2003-11-07 2005-11-09 株式会社日立ハイテクノロジーズ Ｒｎａ抽出方法、および生体材料の分析方法
US20060099567A1 (en) 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
ATE497837T1 (de) 2004-04-09 2011-02-15 Vivebio Llc Vorrichtungen und verfahren für abnahme, lagerung und transport von biologischen proben
DE102004021780B4 (de) 2004-04-30 2008-10-02 Siemens Ag Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien
JP2008509226A (ja) * 2004-05-24 2008-03-27 ジェンボールト コーポレイション 回収可能な形式での安定なタンパク質保管および安定な核酸保管
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
US20090208919A1 (en) 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
CN101175853A (zh) 2005-03-16 2008-05-07 Dna吉诺特克股份有限公司 用于保存来自体液的核酸的组合物和方法
GB0505909D0 (en) 2005-03-23 2005-04-27 Univ Leeds Formulations
US20070015165A1 (en) 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
WO2007070381A2 (en) 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
EP2175999B1 (en) * 2007-06-21 2017-01-04 Gen-Probe Incorporated Receptacles for use in performing processes
US20090053704A1 (en) * 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
JP5726529B2 (ja) * 2007-11-30 2015-06-03 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 単一試料からのゲノムｄｎａ、ｒｎａおよびタンパク質の単離方法
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US20100209957A1 (en) 2008-06-20 2010-08-19 Genvault Corporation Biosample storage devices and methods of use thereof
CN102177237B (zh) 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
WO2010111265A1 (en) * 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
WO2010132508A2 (en) 2009-05-11 2010-11-18 Biomatrica, Inc. Compositions and methods for biological sample storage
ES2639641T3 (es) 2010-04-20 2017-10-27 Octapharma Ag Nuevo agente estabilizante para proteínas farmacéuticas
EP2388312A1 (en) 2010-05-17 2011-11-23 Curetis AG Universally applicable lysis buffer and processing methods for the lysis of bodily samples
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012075471A1 (en) 2010-12-04 2012-06-07 Hui-Yu Liu Rna stability enhancer
US20120152743A1 (en) 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples
GB201103257D0 (en) 2011-02-25 2011-04-13 Ge Healthcare Uk Ltd Paper support and method of recovering biological material therefrom
GB2496122A (en) 2011-10-31 2013-05-08 Ge Healthcare Bio Sciences Ab Biological sample preservation on paper
CN103173432B (zh) * 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Also Published As

Publication number Publication date
EP2844755B1 (en) 2016-11-16
US9044738B2 (en) 2015-06-02
WO2013165870A1 (en) 2013-11-07
JP2018068303A (ja) 2018-05-10
CA2870038A1 (en) 2013-11-07
ES2609763T3 (es) 2017-04-24
EP2844755A1 (en) 2015-03-11
CN104254776B (zh) 2016-09-28
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
IN2014DN08223A (enExample) 2015-05-15
US20130289265A1 (en) 2013-10-31
JP2015518569A (ja) 2015-07-02
CA2870038C (en) 2021-08-03
EP2844755A4 (en) 2015-03-11
JP6847365B2 (ja) 2021-03-24

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