JP6248095B2 - 核酸の抽出及び貯蔵のための方法及び組成物 - Google Patents

核酸の抽出及び貯蔵のための方法及び組成物 Download PDF

Info

Publication number
JP6248095B2
JP6248095B2 JP2015510354A JP2015510354A JP6248095B2 JP 6248095 B2 JP6248095 B2 JP 6248095B2 JP 2015510354 A JP2015510354 A JP 2015510354A JP 2015510354 A JP2015510354 A JP 2015510354A JP 6248095 B2 JP6248095 B2 JP 6248095B2
Authority
JP
Japan
Prior art keywords
solid matrix
rna
nucleic acid
sample
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015510354A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015518569A5 (enExample
JP2015518569A (ja
Inventor
リ，ビン
ムーア，デイヴィッド・ロジャー
クヴァム，エリック・リーミング
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of JP2015518569A publication Critical patent/JP2015518569A/ja
Publication of JP2015518569A5 publication Critical patent/JP2015518569A5/ja
Application granted granted Critical
Publication of JP6248095B2 publication Critical patent/JP6248095B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015510354A 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物 Active JP6248095B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
US13/460,076 2012-04-30
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2017225326A Division JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物

Publications (3)

Publication Number Publication Date
JP2015518569A JP2015518569A (ja) 2015-07-02
JP2015518569A5 JP2015518569A5 (enExample) 2016-06-09
JP6248095B2 true JP6248095B2 (ja) 2017-12-13

Family

ID=49477845

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2015510354A Active JP6248095B2 (ja) 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物
JP2017225326A Active JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物
JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2017225326A Active JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物
JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

Country Status (8)

Country Link
US (1) US9044738B2 (enExample)
EP (1) EP2844755B1 (enExample)
JP (3) JP6248095B2 (enExample)
CN (1) CN104254776B (enExample)
CA (1) CA2870038C (enExample)
ES (1) ES2609763T3 (enExample)
IN (1) IN2014DN08223A (enExample)
WO (1) WO2013165870A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US10829291B2 (en) * 2012-06-20 2020-11-10 Thermo Fischer Scientific Baltics UAB Method to prevent silica-based column aging
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
CN107076764A (zh) * 2014-11-20 2017-08-18 通用电气医疗集团英国有限公司 检测稳定在固体载体材料上的痴呆和阿尔茨海默病相关生物标记
GB201505516D0 (en) * 2015-03-31 2015-05-13 Ge Healthcare Uk Ltd Improvements in and relating to biological sample collectors and handling thereof
EP3754011B1 (en) 2015-09-09 2022-02-16 Drawbridge Health, Inc. Devices for sample collection, stabilization and preservation
US10000742B2 (en) * 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
KR102589056B1 (ko) 2015-12-08 2023-10-12 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
ES2952950T3 (es) * 2016-03-18 2023-11-07 Hummingbird Diagnostics Gmbh Purificación de fracciones de ARN utilizando un material polimérico hidrofílico
CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
US11166658B2 (en) * 2016-07-28 2021-11-09 Invitae Corporation Blood sampling system and method
WO2018081161A1 (en) * 2016-10-24 2018-05-03 Biomatrica, Inc. Stabilization of nucleic acids on paper
CN115251923A (zh) 2017-01-10 2022-11-01 集联健康有限公司 用于样品收集的装置、系统和方法
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
CN113227795B (zh) * 2019-01-25 2024-09-03 积水化学工业株式会社 核酸的分离方法、核酸的分离试剂盒和检测芯片
US20230303995A1 (en) * 2020-05-14 2023-09-28 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
JP7603552B2 (ja) * 2020-08-28 2024-12-20 花王株式会社 Ｒｎａの保存方法
WO2022198086A1 (en) * 2021-03-19 2022-09-22 Becton, Dickinson And Company Isothermal amplification of pathogens
GB202211645D0 (en) 2022-08-09 2022-09-21 Hemodx As Kit
SE548123C2 (en) * 2023-11-08 2026-03-31 Sequrna Ab Device for dry storage of biological samples, conserving RNA from degradation and use of such devices

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4540506A (en) 1983-04-15 1985-09-10 Genex Corporation Composition for cleaning drains clogged with deposits containing hair
CA1255197A (en) * 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
US5173422A (en) 1986-08-22 1992-12-22 Miles Inc. Monoclonal antibodies specific for human glycoalbumin
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
US5756126A (en) 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
DE69633239T2 (de) 1995-06-07 2005-09-08 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
JPH11510393A (ja) 1995-08-09 1999-09-14 インスティチュート ナショナル ドゥ ラ セント エト ドゥ ラ リサーチェ メディカル 白血病マーカーとしておよび乳癌の予後において有用な単離された核酸分子
US5939259A (en) 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US20010039010A1 (en) 1998-09-03 2001-11-08 Leigh Alexander Burgoyne Sample collection medium incorporating material for sample visualization
WO2000066606A1 (en) 1999-04-30 2000-11-09 Whatman, Inc. Substrate including anionic detergent for purifying nucleic acid
ATE347616T1 (de) 1999-04-30 2006-12-15 Cyclops Genome Sciences Ltd Ribonukleinsäurederivate
US6664379B1 (en) 1999-09-24 2003-12-16 Ambion, Inc. Nuclease inhibitor cocktail
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US7531362B2 (en) 2001-06-07 2009-05-12 Medmira Inc. Rapid diagnostic assay
EP1423514A2 (en) 2001-09-05 2004-06-02 WHATMAN plc Stable storage of proteins
GB0129816D0 (en) * 2001-12-13 2002-01-30 The Technology Partnership Plc Testing device for chemical or biochemical analysis
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040126783A1 (en) 2002-07-10 2004-07-01 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
DE10231659B4 (de) 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
US7267950B2 (en) 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
EP1628905A1 (en) * 2003-05-23 2006-03-01 Gyros Patent Ab Hydrophilic/hydrophobic surfaces
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
EP1641944B1 (en) 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
JP3714940B2 (ja) 2003-11-07 2005-11-09 株式会社日立ハイテクノロジーズ Ｒｎａ抽出方法、および生体材料の分析方法
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
ES2360801T3 (es) 2004-04-09 2011-06-09 Vivebio, Llc Dispositivos y métodos para la recogida, almacenamiento y transporte de muestras biológicas.
DE102004021780B4 (de) 2004-04-30 2008-10-02 Siemens Ag Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien
JP2008509226A (ja) * 2004-05-24 2008-03-27 ジェンボールト コーポレイション 回収可能な形式での安定なタンパク質保管および安定な核酸保管
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
US20090208919A1 (en) 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
CA2600758C (en) 2005-03-16 2016-01-12 Dna Genotek Inc. Compositions and method for storage of nucleic acid from bodily fluids
GB0505909D0 (en) 2005-03-23 2005-04-27 Univ Leeds Formulations
US20070015165A1 (en) 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
WO2007070381A2 (en) 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
US8163535B2 (en) 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
JP5282088B2 (ja) * 2007-06-21 2013-09-04 ジェン−プロウブ インコーポレイテッド 処理実行に使用する機器および容器
US20090053704A1 (en) 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
AU2008329833B2 (en) * 2007-11-30 2014-04-17 Global Life Sciences Solutions Usa Llc Method for isolation of genomic DNA, RNA and proteins from a single sample
WO2009155612A2 (en) 2008-06-20 2009-12-23 Genvault Corporation Sample collection and storage devices and methods of use thereof
CN102177237B (zh) 2008-09-12 2013-10-30 金沃特公司 用于贮存和稳定生物分子的基质和介质
CA2756463C (en) * 2009-03-24 2019-01-22 University Of Chicago Slip chip device and methods
EP2430195B1 (en) 2009-05-11 2019-01-23 Biomatrica, INC. Compositions and methods for biological sample storage
CN103003422B (zh) 2010-04-20 2015-02-11 奥克塔法马股份有限公司 用于药物蛋白的新型稳定剂
EP2388312A1 (en) 2010-05-17 2011-11-23 Curetis AG Universally applicable lysis buffer and processing methods for the lysis of bodily samples
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
CN103370412B (zh) 2010-12-04 2015-07-22 万芬 核糖核酸稳定性增强剂
US20120152743A1 (en) 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples
GB201103257D0 (en) 2011-02-25 2011-04-13 Ge Healthcare Uk Ltd Paper support and method of recovering biological material therefrom
GB2496122A (en) 2011-10-31 2013-05-08 Ge Healthcare Bio Sciences Ab Biological sample preservation on paper
CN103173432B (zh) * 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Also Published As

Publication number Publication date
EP2844755A1 (en) 2015-03-11
EP2844755B1 (en) 2016-11-16
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
JP6847365B2 (ja) 2021-03-24
EP2844755A4 (en) 2015-03-11
US9044738B2 (en) 2015-06-02
JP2015518569A (ja) 2015-07-02
CN104254776B (zh) 2016-09-28
WO2013165870A1 (en) 2013-11-07
CA2870038A1 (en) 2013-11-07
US20130289265A1 (en) 2013-10-31
JP2018068303A (ja) 2018-05-10
ES2609763T3 (es) 2017-04-24
IN2014DN08223A (enExample) 2015-05-15
CA2870038C (en) 2021-08-03

Similar Documents

Publication Publication Date Title
JP6847365B2 (ja) 核酸の抽出及び貯蔵のための方法及び組成物
US9040675B2 (en) Formulations for nucleic acid stabilization on solid substrates
JP6544860B2 (ja) 固体基材上での核酸安定化のための配合物
CA2921286C (en) Methods and compositions for extraction and storage of nucleic acids
US9040679B2 (en) Methods and compositions for extraction and storage of nucleic acids
DK2539449T3 (en) PROCEDURE FOR PARALLEL ISOLATION AND CLEANING RNA AND DNA
CN103370412B (zh) 核糖核酸稳定性增强剂
US9534214B2 (en) Substrates and associated methods for elution of nucleic acids
WO2014143714A2 (en) Methods for one step nucleic acid amplification of non-eluted samples
JP7560112B2 (ja) Ｐｖａスポンジを用いるリキッドバイオプシーの調製方法
Sharma et al. Ribonucleic acid extraction from archival formalin fixed paraffin embedded myocardial tissues for gene expression and pathogen detection
WO2015108597A2 (en) Substrates and associated methods for elution of nucleic acids
Barra et al. B-215 Evaluating RT-qPCR for Dengue Virus Detection: Outcomes of a Brazilian External Quality Assessment Program
CN119020463A (zh) 一种动物毛发样本基因组dna文库的构建方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160414

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160414

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170119

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170207

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170428

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20170707

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170804

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20171024

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20171120

R150 Certificate of patent or registration of utility model

Ref document number: 6248095

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

S111 Request for change of ownership or part of ownership

Free format text: JAPANESE INTERMEDIATE CODE: R313113

R350 Written notification of registration of transfer

Free format text: JAPANESE INTERMEDIATE CODE: R350

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250