CN104232622B - 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 - Google Patents
一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 Download PDFInfo
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- CN104232622B CN104232622B CN201410535579.1A CN201410535579A CN104232622B CN 104232622 B CN104232622 B CN 104232622B CN 201410535579 A CN201410535579 A CN 201410535579A CN 104232622 B CN104232622 B CN 104232622B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
引物名称 | 序列(5’-3’) |
FP | ACGATTCGTACATAGAAGTATAG-GGTCGATACCGCCTGGAC(序列表中序列2) |
BP | GATATGAAGATACATGCTTAGCA-GCATGCAGCGCGTCCA(序列表中序列3) |
引物名称 | 序列(5’-3’) |
FP | ACGATTCGTACATAGAAGTATAG-GCAATGAGAACTATTGGGACTC(序列表中序列5) |
BP | GATATGAAGATACATGCTTAGCA-ATTTGCTGCAATGACGAGAG(序列表中序列6) |
Claims (8)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410535579.1A CN104232622B (zh) | 2014-09-24 | 2014-10-11 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
US15/518,480 US10544452B2 (en) | 2014-09-24 | 2015-07-07 | Method and use of nucleic acid isothermal amplification via a polymerase spiral reaction |
PCT/CN2015/000493 WO2016054870A1 (zh) | 2014-09-24 | 2015-07-07 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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CN2014104923025 | 2014-09-24 | ||
CN201410492302.5 | 2014-09-24 | ||
CN201410492302 | 2014-09-24 | ||
CN201410535579.1A CN104232622B (zh) | 2014-09-24 | 2014-10-11 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN104232622A CN104232622A (zh) | 2014-12-24 |
CN104232622B true CN104232622B (zh) | 2016-09-14 |
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CN201410535579.1A Active CN104232622B (zh) | 2014-09-24 | 2014-10-11 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
Country Status (3)
Country | Link |
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US (1) | US10544452B2 (zh) |
CN (1) | CN104232622B (zh) |
WO (1) | WO2016054870A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112980927A (zh) * | 2021-03-23 | 2021-06-18 | 海南微氪生物科技股份有限公司 | 一种基于聚合酶螺旋反应的扩增核酸方法 |
Families Citing this family (28)
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EP2861996B1 (en) | 2013-03-11 | 2019-03-06 | Cue Health Inc. | Sample analysis cartridge |
US9623409B2 (en) | 2013-03-11 | 2017-04-18 | Cue Inc. | Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes |
US10545161B2 (en) | 2013-03-11 | 2020-01-28 | Cue Health Inc. | Systems and methods for detection and quantification of analytes |
USD745423S1 (en) | 2014-05-12 | 2015-12-15 | Cue Inc. | Automated analyzer test cartridge and sample collection device for analyte detection |
CN104232622B (zh) | 2014-09-24 | 2016-09-14 | 中国人民解放军疾病预防控制所 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
CN104975013A (zh) * | 2015-07-07 | 2015-10-14 | 中国人民解放军疾病预防控制所 | 一种加速聚合酶螺旋反应的方法及其应用 |
EP3325153B1 (en) | 2015-07-17 | 2020-03-18 | Cue Health Inc. | Sample analysis cartridge |
CN105219874A (zh) * | 2015-11-04 | 2016-01-06 | 中国人民解放军疾病预防控制所 | 铜绿假单胞菌的psr检测方法及其专用引物与试剂盒 |
CN105400875B (zh) * | 2015-12-02 | 2018-12-21 | 上海市计量测试技术研究院 | 一套用于细菌耐药基因ndm-1检测的多核苷酸、方法和试剂盒 |
CN107119040A (zh) * | 2016-02-24 | 2017-09-01 | 青岛艾菲生物技术有限公司 | 一种等温核酸扩增的方法 |
CN108130270A (zh) * | 2016-12-01 | 2018-06-08 | 李治国 | 即用型基因检测包 |
WO2018132939A1 (zh) * | 2017-01-17 | 2018-07-26 | 中国科学院过程工程研究所 | 一种恒温条件下合成核酸的方法 |
CN106636071B (zh) * | 2017-01-17 | 2020-07-17 | 中国科学院过程工程研究所 | 一种恒温条件下合成核酸的方法 |
US11237161B2 (en) | 2017-01-25 | 2022-02-01 | Cue Health Inc. | Systems and methods for enhanced detection and quantification of analytes |
CN107746898A (zh) * | 2017-11-06 | 2018-03-02 | 北京市水产技术推广站 | 一种检测鲤鱼浮肿病毒的检测试剂盒和检测方法 |
CN108315390A (zh) * | 2018-03-15 | 2018-07-24 | 吉林大学 | 用于lamp检测的可视化混合染料 |
CN108588196B (zh) * | 2018-04-23 | 2021-12-14 | 北京中能通达科技发展中心(有限合伙) | 一种防止pcr形成气溶胶污染的方法 |
CN108796098A (zh) * | 2018-06-13 | 2018-11-13 | 华南理工大学 | 一种psr等温扩增反应检测大肠杆菌志贺毒素的引物、试剂盒及其方法 |
CN109182596A (zh) * | 2018-08-06 | 2019-01-11 | 南阳师范学院 | 一种猪流行性腹泻病毒的反转录聚合酶螺旋反应等温检测试剂及检测方法 |
CN109355403B (zh) * | 2018-08-10 | 2021-03-30 | 华南理工大学 | 一种psr检测耐甲氧西林金葡菌的引物、试剂盒与方法 |
CN110628950B (zh) * | 2019-10-10 | 2022-06-07 | 中国检验检疫科学研究院 | 一种用于检测ev71病毒的引物组合、试剂盒和psr方法 |
CN110643738A (zh) * | 2019-10-10 | 2020-01-03 | 中国检验检疫科学研究院 | 一种用于检测甲型h1n1流感病毒的引物组合、试剂盒和psr方法 |
CN110628948B (zh) * | 2019-10-10 | 2022-03-29 | 中国检验检疫科学研究院 | 一种用于检测黄热病毒的引物组合、试剂盒和psr方法 |
CN110699437B (zh) * | 2019-11-11 | 2023-06-20 | 益善生物技术股份有限公司 | 人sdc2基因甲基化检测试剂盒 |
CN112695110A (zh) * | 2020-12-29 | 2021-04-23 | 复旦大学 | 一种聚合酶螺旋反应快速检测肺炎链球菌核酸的引物组、试剂盒及其应用 |
CN112662822B (zh) * | 2021-02-26 | 2022-06-14 | 甘肃农业大学 | 一种基于聚合酶螺旋反应检测猫细小病毒的引物组、试剂及其方法 |
CN113528626B (zh) * | 2021-07-01 | 2023-04-11 | 长沙智飞生物科技有限公司 | 一种合成核酸的方法 |
CN114045330B (zh) * | 2021-12-23 | 2024-02-09 | 川北医学院附属医院 | 一种基于滑动复制的核酸等温扩增方法 |
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US6221635B1 (en) * | 1999-05-06 | 2001-04-24 | The Wistar Institute | Methods for solid-phase amplification of DNA template (SPADT) using multiarrays |
ES2322559T3 (es) * | 2001-02-27 | 2009-06-23 | Virco Bvba | Amplificacion de sonda circular (cpa) de moleculas de acido nucleico circularizadas. |
JP5026958B2 (ja) * | 2004-06-01 | 2012-09-19 | アリーア サン ディエゴ, インコーポレイテッド | リコンビナーゼポリメラーゼ増幅 |
CN102260733A (zh) * | 2010-05-26 | 2011-11-30 | 浙江省血液中心 | 加速引物的设计方法、靶分子的检测方法与检测用试剂盒 |
CN102242197B (zh) * | 2011-05-19 | 2013-05-22 | 中国人民解放军疾病预防控制所 | 用于检测ndm-1基因的lamp试剂盒及其专用引物 |
US9523120B2 (en) * | 2011-06-14 | 2016-12-20 | University of Pittsburgh—of the Commonwealth System of Higher Education | Method of amplifying a nucleic acid |
CN102399901B (zh) * | 2011-12-21 | 2013-07-24 | 中国人民解放军疾病预防控制所 | 结核杆菌的lamp检测方法及其专用引物与试剂盒 |
CN102936623B (zh) * | 2012-09-28 | 2014-11-19 | 中国人民解放军疾病预防控制所 | 用于核酸恒温扩增反应中防止核酸污染和指示其反应结果的方法 |
CN103540660B (zh) * | 2013-10-10 | 2015-10-28 | 中国人民解放军疾病预防控制所 | 基于TaqMan探针的环介导恒温扩增法及其专用LAMP引物与试剂盒 |
CN103525949A (zh) * | 2013-10-15 | 2014-01-22 | 中国人民解放军疾病预防控制所 | 用于检测h7n9病毒ha基因和na基因的rt-lamp引物组合及试剂盒 |
CN104232622B (zh) | 2014-09-24 | 2016-09-14 | 中国人民解放军疾病预防控制所 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
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2014
- 2014-10-11 CN CN201410535579.1A patent/CN104232622B/zh active Active
-
2015
- 2015-07-07 US US15/518,480 patent/US10544452B2/en not_active Expired - Fee Related
- 2015-07-07 WO PCT/CN2015/000493 patent/WO2016054870A1/zh active Application Filing
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112980927A (zh) * | 2021-03-23 | 2021-06-18 | 海南微氪生物科技股份有限公司 | 一种基于聚合酶螺旋反应的扩增核酸方法 |
Also Published As
Publication number | Publication date |
---|---|
US10544452B2 (en) | 2020-01-28 |
CN104232622A (zh) | 2014-12-24 |
US20170226574A1 (en) | 2017-08-10 |
WO2016054870A1 (zh) | 2016-04-14 |
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Application publication date: 20141224 Assignee: BEIJING ANJIEYOU TECHNOLOGY CO., LTD. Assignor: Institute of Disease Control and Prevention of PLA Contract record no.: 2018990000268 Denomination of invention: Nucleic acid isothermal amplification method and application thereof by polymerase spiral reaction Granted publication date: 20160914 License type: Exclusive License Record date: 20181009 |
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Address after: 100071 No. 20 East Main Street, Beijing, Fengtai District Patentee after: CHINESE PLA CENTER FOR DISEASE CONTROL & PREVENTION Address before: 100071 No. 20 East Main Street, Beijing, Fengtai District Patentee before: INSTITUTE OF DISEASE CONTROL AND PREVENTION OF PLA |
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Assignee: BEIJING ANJIEYOU TECHNOLOGY Co.,Ltd. Assignor: INSTITUTE OF DISEASE CONTROL AND PREVENTION OF PLA Contract record no.: 2018990000268 Date of cancellation: 20210915 |
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