CN104232622A - 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 - Google Patents
一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 Download PDFInfo
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- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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Abstract
Description
引物名称 | 序列(5’-3’) |
FP | ACGATTCGTACATAGAAGTATAG-GGTCGATACCGCCTGGAC(序列表中序列2) |
BP | GATATGAAGATACATGCTTAGCA-GCATGCAGCGCGTCCA(序列表中序列3) |
引物名称 | 序列(5’-3’) |
FP | ACGATTCGTACATAGAAGTATAG-GCAATGAGAACTATTGGGACTC(序列表中序列5) |
BP | GATATGAAGATACATGCTTAGCA-ATTTGCTGCAATGACGAGAG(序列表中序列6) |
Claims (8)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410535579.1A CN104232622B (zh) | 2014-09-24 | 2014-10-11 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
PCT/CN2015/000493 WO2016054870A1 (zh) | 2014-09-24 | 2015-07-07 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
US15/518,480 US10544452B2 (en) | 2014-09-24 | 2015-07-07 | Method and use of nucleic acid isothermal amplification via a polymerase spiral reaction |
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CN201410492302.5 | 2014-09-24 | ||
CN2014104923025 | 2014-09-24 | ||
CN201410492302 | 2014-09-24 | ||
CN201410535579.1A CN104232622B (zh) | 2014-09-24 | 2014-10-11 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
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CN104232622A true CN104232622A (zh) | 2014-12-24 |
CN104232622B CN104232622B (zh) | 2016-09-14 |
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US (1) | US10544452B2 (zh) |
CN (1) | CN104232622B (zh) |
WO (1) | WO2016054870A1 (zh) |
Cited By (26)
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CN104975013A (zh) * | 2015-07-07 | 2015-10-14 | 中国人民解放军疾病预防控制所 | 一种加速聚合酶螺旋反应的方法及其应用 |
CN105219874A (zh) * | 2015-11-04 | 2016-01-06 | 中国人民解放军疾病预防控制所 | 铜绿假单胞菌的psr检测方法及其专用引物与试剂盒 |
CN105400875A (zh) * | 2015-12-02 | 2016-03-16 | 上海市计量测试技术研究院 | 一套用于细菌耐药基因ndm-1检测的多核苷酸、方法和试剂盒 |
WO2016054870A1 (zh) * | 2014-09-24 | 2016-04-14 | 中国人民解放军疾病预防控制所 | 一种用聚合酶螺旋反应恒温扩增核酸的方法及其应用 |
US9623409B2 (en) | 2013-03-11 | 2017-04-18 | Cue Inc. | Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes |
CN106636071A (zh) * | 2017-01-17 | 2017-05-10 | 中国科学院过程工程研究所 | 一种恒温条件下合成核酸的方法 |
USD789815S1 (en) | 2014-05-12 | 2017-06-20 | Cue Inc. | Reader of an analyte detection system |
US9718058B2 (en) | 2015-07-17 | 2017-08-01 | Cue Inc. | Cartridges, kits, and methods for enhanced detection and quantification of analytes |
WO2017143873A1 (zh) * | 2016-02-24 | 2017-08-31 | 青岛艾菲生物技术有限公司 | 一种等温核酸扩增的方法 |
CN107746898A (zh) * | 2017-11-06 | 2018-03-02 | 北京市水产技术推广站 | 一种检测鲤鱼浮肿病毒的检测试剂盒和检测方法 |
CN108130270A (zh) * | 2016-12-01 | 2018-06-08 | 李治国 | 即用型基因检测包 |
CN108315390A (zh) * | 2018-03-15 | 2018-07-24 | 吉林大学 | 用于lamp检测的可视化混合染料 |
WO2018132939A1 (zh) * | 2017-01-17 | 2018-07-26 | 中国科学院过程工程研究所 | 一种恒温条件下合成核酸的方法 |
CN108588196A (zh) * | 2018-04-23 | 2018-09-28 | 北京中能通达科技发展中心(有限合伙) | 一种防止pcr形成气溶胶污染的方法 |
CN108796098A (zh) * | 2018-06-13 | 2018-11-13 | 华南理工大学 | 一种psr等温扩增反应检测大肠杆菌志贺毒素的引物、试剂盒及其方法 |
CN109182596A (zh) * | 2018-08-06 | 2019-01-11 | 南阳师范学院 | 一种猪流行性腹泻病毒的反转录聚合酶螺旋反应等温检测试剂及检测方法 |
US10195606B2 (en) | 2013-03-11 | 2019-02-05 | Cue Health Inc. | Systems and methods for detection and quantification of analytes |
CN109355403A (zh) * | 2018-08-10 | 2019-02-19 | 华南理工大学 | 一种psr检测耐甲氧西林金葡菌的引物、试剂盒与方法 |
CN110628948A (zh) * | 2019-10-10 | 2019-12-31 | 中国检验检疫科学研究院 | 一种用于检测黄热病毒的引物组合、试剂盒和psr方法 |
CN110643738A (zh) * | 2019-10-10 | 2020-01-03 | 中国检验检疫科学研究院 | 一种用于检测甲型h1n1流感病毒的引物组合、试剂盒和psr方法 |
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US10545161B2 (en) | 2013-03-11 | 2020-01-28 | Cue Health Inc. | Systems and methods for detection and quantification of analytes |
CN112662822A (zh) * | 2021-02-26 | 2021-04-16 | 甘肃农业大学 | 一种基于聚合酶螺旋反应检测猫细小病毒的引物组、试剂及其方法 |
CN112695110A (zh) * | 2020-12-29 | 2021-04-23 | 复旦大学 | 一种聚合酶螺旋反应快速检测肺炎链球菌核酸的引物组、试剂盒及其应用 |
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CN112980927A (zh) * | 2021-03-23 | 2021-06-18 | 海南微氪生物科技股份有限公司 | 一种基于聚合酶螺旋反应的扩增核酸方法 |
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2014
- 2014-10-11 CN CN201410535579.1A patent/CN104232622B/zh not_active Expired - Fee Related
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2015
- 2015-07-07 US US15/518,480 patent/US10544452B2/en not_active Expired - Fee Related
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US10544452B2 (en) | 2020-01-28 |
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US20170226574A1 (en) | 2017-08-10 |
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