CN104221840B - The artificial raise seedling method of a kind of Leizhou sargassum - Google Patents
The artificial raise seedling method of a kind of Leizhou sargassum Download PDFInfo
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- CN104221840B CN104221840B CN201410522200.3A CN201410522200A CN104221840B CN 104221840 B CN104221840 B CN 104221840B CN 201410522200 A CN201410522200 A CN 201410522200A CN 104221840 B CN104221840 B CN 104221840B
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- culture fluid
- sargassum
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- leizhou
- algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses the artificial raise seedling method of a kind of Leizhou sargassum, it is characterized in that: by (1) collection horse hair algae, the pretreatment of (2) kind algae, (3) isolate receptacle, (4) suppress Spawning, the smart ovum of (5) promotion discharge is also fertilized and (6) nursery six steps form, described (4) suppression Spawning: at intensity of illumination 6-10 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and controls culture fluid water temperature and remain on 5-10 DEG C, suppress Spawning to continue 15-60 days.The artificial raise seedling method of a kind of Leizhou of the present invention sargassum, kind of an algae resource can be saved, postpone the time that sargassum receptacle discharges smart ovum, evening collects seedling, thus effectively shortening seedling is aestivated the time, reduce the labour intensity of management of aestivating, improve the total amount of ejaculation, ovum, reduce costs and nursery risk, improve nursery success rate and total amount.
Description
Technical field
The invention belongs to sargassum seedling growing process field, specifically, relate to the artificial raise seedling method of a kind of Leizhou sargassum.
Background technology
In recent years, marine environmental quality declined, and sea jungle fails increasingly, and constantly increasing as the algin demand of essential industry raw material, the reparation of marine ecosystems, and the structure of oceanic pasture, only rely on the algae field resource of existing self-sow, can not meet the demand of people.At present, the natural resources of Leizhou sargassum affect and reduce year by year with being also subject to nature or human factor, carry out propagate artificially extremely urgent.
The domestic research to this algae cultivation aspect launches, and to have studied in its nursery the control of assorted algae and sex ration, impact that density grows its juvenile sporophyte, but seedling problem never solves, and seeling industry is the key link of restriction scale artificial culture.
Under natural environment, Leizhou sargassum winter Mo early summer is ripe, grows after the sperm diffused, ovum fertilization, plant algae then dead, run off, and after juvenile sporophyte aestivates, wait the just continued growth of water temperature drop in autumn.When propagating artificially, according to the spontaneous growth rule of Leizhou sargassum, seedling time of aestivating is longer, and at a time when typhoon season, substantially increases the cost and risk of artificial breeding, expends larger labour.
The seedling growing process that delay Leizhou sargassum receptacle discharges smart ovum can be collected seedling evening, thus effectively shortening seedling is aestivated the time, reduces the labour intensity of management of aestivating, reduces costs.
Summary of the invention
For solving above technical problem, the object of the present invention is to provide the artificial raise seedling method of a kind of Leizhou sargassum, can collect seedling evening, shorten seedling and aestivate the time, reduce the labour intensity of management of aestivating, reduce costs and nursery risk, improve nursery success rate.
One, the object of the present invention is achieved like this: the artificial raise seedling method of a kind of Leizhou sargassum, and its key is: by (1) collection horse hair algae, the pretreatment of (2) kind algae, (3) isolate receptacle, (4) suppress Spawning, the smart ovum of (5) promotion discharge is also fertilized and (6) nursery six steps form:
Wherein said step (1) gathers horse hair algae and is specially: gather ripe at the beginning of last month of spring in winter but not yet discharge that smart ovum, stalwartness, side shoot are many, the Leizhou sargassum of hardly staining metal or nothing as kind of an algae, male and female packing, and take Transportation.
The pretreatment that described step (2) plants algae is specially: by the assorted algae on kind of algae and attachment removing in culture fluid.
Described step (3) is isolated receptacle and is specially: in the kind algae that step (2) obtains, isolate stalwartness respectively, grow one and to make peace many female, the male receptacle of side shoot, the attachment on receptacle surface is removed in culture fluid, again receptacle is separated into single or Dan Cong, is placed in scalding, cooling, the in advance warm seawater processed are deposited.
Described step (4) suppresses Spawning to be specially: in illumination box or culturing room, at intensity of illumination 6-10 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and control culture fluid water temperature and remain on 5-10 DEG C, every day, a culture fluid was changed in timing, and every day timing agitation or shake culture fluid 3 times, suppress Spawning to continue 15-60 days.
Described step (5) promotes the smart ovum of discharge and fertilization is specially: mixed by female, the male receptacle 1:1 in step (4), proceed to 23 DEG C ~ 30 DEG C, salinity is in the culture fluid of 22 ~ 34, and at intensity of illumination 40 ~ 120 μm of olm
-2s
-1, under photoperiod 12L:12D condition, quiescent culture, every day, a culture fluid was changed in timing, and timing shake culture fluid 3 times, and each shake 0.5 minute, cultivates 6-12 days.
Described step (6) nursery is specially: step (5) is cultivated the fertilized egg obtained and proceed to that temperature is 23 DEG C, salinity is in the culture fluid of 30 again, and be 120 μm of olm in intensity of illumination
-2s
-1, the photoperiod is under the condition of 12L:12D, cultivates described fertilized egg, cultivates through 15-21 days, obtains the seedling with 1-3 blade.
Preferably above-mentioned steps (4) suppresses Spawning: suppress Spawning to continue 45-60 days.
Above-mentioned culture fluid is the seawater of via hole diameter 50 μm of strainer filterings.
Two, female receptacle suppressed under 5 DEG C and 10 DEG C of low temperature rear and directly do not suppress to carry out cultivation comparing result under 23 DEG C of conditions: as shown in Figure 2,3, 4:
Fig. 2: illustrate that the average ovulation fossa hole count of each female holder after suppressing under 5 DEG C ~ 10 DEG C low temperature obviously increases under not suppressing (directly at 23 DEG C) condition, and temperature is lower, the increase of ovulation fossa hole count is more obvious, and suppresses the time longer, and ovulation fossa hole count is more.
Fig. 3: illustrate that the average number of eggs ovulated of each socket bore of each female holder after suppressing under 5 DEG C ~ 10 DEG C low temperature is than less slightly under not suppressing (directly at 23 DEG C) condition, namely low temperature suppression causes the number of eggs ovulated of single socket bore slightly to decline.
Fig. 4: illustrate that 5 DEG C of low temperature suppress 30-60 days, 10 DEG C low temperature to suppress the average ovulation sum of each female holder in 45 ~ 60 days obviously to increase under not suppressing (directly at 23 DEG C-control group) condition, but 5 DEG C of low temperature suppress 15 days, 10 DEG C low temperature to suppress the average ovulation sum of each female holder in 15 ~ 30 days to reduce to some extent.Therefore the suppression number of days that the present invention is best under 5 DEG C ~ 10 DEG C low temperature is 45 ~ 60 days, and the average ovulation sum of each female holder obviously increases, and temperature is lower, and ovulation sum increases more obvious.
In sum: when 5 DEG C of low temperature suppress to cultivate, along with incubation time extends, the average ovulation fossa hole count of receptacle of sargassum increases gradually, but the average number of eggs ovulated of each socket bore reduces slightly, the ovulation sum of each female holder except slightly declining under suppression 15 days conditions, under suppression 30 ~ 60 days conditions, the ovulation sum of each female holder increases gradually along with suppressing the prolongation of time, all fairly obvious relative to control group increase.When 10 DEG C of low temperature suppress to cultivate, along with incubation time extends, the average ovulation fossa hole count of receptacle of sargassum increases gradually, but the ovulation of the average number of eggs ovulated of each socket bore and each female holder sum reduces after 15 ~ 30 days gradually in cultivation, and suppressing 45 ~ 60 days, the average number of eggs ovulated of each socket bore and the ovulation sum of each female holder are all apparently higher than control group.In a word, under 5 DEG C ~ 10 DEG C low temperature suppress, the in vitro receptacle of Leizhou sargassum shows a procedure of adaptation to low temperature within a certain period of time, and ovulation sum can decline slightly, but along with suppressing the prolongation of incubation time, the ovulation sum of each in vitro female holder obviously increases.
Beneficial effect:
The artificial raise seedling method of a kind of Leizhou of the present invention sargassum, kind of an algae resource can be saved, postpone the time that sargassum receptacle discharges smart ovum, can collect seedling evening, thus effectively shortening seedling is aestivated the time, reduce the labour intensity of management of aestivating, improve the total amount of ejaculation, ovum, reduce costs and nursery risk, improve nursery success rate and total amount.
Accompanying drawing illustrates:
Fig. 1 is flow chart of the present invention;
Fig. 2 is the comparison diagram of each female receptacle average ovulation fossa hole count when suppressing and not suppressing;
Fig. 3 is the average number of eggs ovulated of each female receptacle each socket bore when suppressing and not suppressing;
Fig. 4 is the comparison diagram of each female receptacle average sum of ovulating when suppressing and not suppressing.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1:
As shown in Figure 1: the artificial raise seedling method of a kind of Leizhou sargassum, gather horse hair algae by (1), (2) plant algae pretreatment, (3) isolate receptacle, (4) suppress Spawning, (5) promote the smart ovum of discharge and fertilization and (6) nursery six steps form:
(1) gather horse hair algae: gather ripe at the beginning of last month of spring in winter but not yet discharge that smart ovum, stalwartness, side shoot are many, the Leizhou sargassum of hardly staining metal or nothing as kind of an algae, male and female packing, and take Transportation.
(2) pretreatment of algae is planted: the assorted algae on kind of algae and attachment are brushed with clean writing brush in culture fluid.
(3) receptacle is isolated: in the kind algae that step (2) obtains, isolate stalwartness respectively with sterilization blade, grow one and to make peace many female, the male receptacle of side shoot, the attachment on receptacle surface is removed in culture fluid, again receptacle is separated into single or Dan Cong, is placed in scalding, cooling, the in advance warm seawater processed are deposited.
(4) Spawning is suppressed: in illumination box or culturing room, at intensity of illumination 6 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and controls culture fluid water temperature and remain on 5 DEG C, every day, a culture fluid was changed in timing, and every day timing agitation or shake culture fluid 3 times, continue 60 days.
(5) the smart ovum of discharge is promoted and fertilization is specially: mixed by female, the male receptacle 1:1 in step (4), proceed to 23 DEG C, salinity is in the culture fluid of 34, and at intensity of illumination 120 μm of olm
-2s
-1, under photoperiod 12L:12D condition, quiescent culture, every day, a culture fluid was changed in timing, and timing shake culture fluid 3 times, and each shake 0.5 minute, cultivates 6 days.
(6) nursery is specially: step (5) is cultivated the fertilized egg obtained and proceed to that temperature is 23 DEG C, salinity is in the culture fluid of 30 again, and be 120 μm of olm in intensity of illumination
-2s
-1, the photoperiod is under the condition of 12L:12D, cultivates described fertilized egg, cultivates through 21 days, obtain and be about 1.5mm, there is the seedling of 1-3 blade.
Embodiment 2:
As shown in Figure 1: the artificial raise seedling method of a kind of Leizhou sargassum, gather horse hair algae by (1), (2) plant algae pretreatment, (3) isolate receptacle, (4) suppress Spawning, (5) promote the smart ovum of discharge and fertilization and (6) nursery six steps form:
(1) gather horse hair algae: gather ripe at the beginning of last month of spring in winter but not yet discharge that smart ovum, stalwartness, side shoot are many, the Leizhou sargassum of hardly staining metal or nothing as kind of an algae, male and female packing, and take Transportation.
(2) pretreatment of algae is planted: the assorted algae on kind of algae and attachment are brushed with clean writing brush in culture fluid.
(3) receptacle is isolated: in the kind algae that step (2) obtains, isolate stalwartness respectively with sterilization blade, grow one and to make peace many female, the male receptacle of side shoot, the attachment on receptacle surface is removed in culture fluid, again receptacle is separated into single or Dan Cong, is placed in scalding, cooling, the in advance warm seawater processed are deposited.
(4) Spawning is suppressed: in illumination box or culturing room, at intensity of illumination 10 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and controls culture fluid water temperature and remain on 10 DEG C, every day, a culture fluid was changed in timing, and every day timing agitation or shake culture fluid 3 times, continue 45 days.
(5) the smart ovum of discharge is promoted and fertilization is specially: mixed by female, the male receptacle 1:1 in step (4), proceed to 30 DEG C, salinity is in the culture fluid of 22, and at intensity of illumination 100 μm of olm
-2s
-1, under photoperiod 12L:12D condition, quiescent culture, every day, a culture fluid was changed in timing, and timing shake culture fluid 3 times, and each shake 0.5 minute, cultivates 9 days.
(6) nursery is specially: step (5) is cultivated the fertilized egg obtained and proceed to that temperature is 23 DEG C, salinity is in the culture fluid of 30 again, and be 120 μm of olm in intensity of illumination
-2s
-1, the photoperiod is under the condition of 12L:12D, cultivates described fertilized egg, cultivates through 15 days, obtain and be about 1.5mm, there is the seedling of 1-3 blade.
Embodiment 3
As shown in Figure 1: the artificial raise seedling method of a kind of Leizhou sargassum, gather horse hair algae by (1), (2) plant algae pretreatment, (3) isolate receptacle, (4) suppress Spawning, (5) promote the smart ovum of discharge and fertilization and (6) nursery six steps form:
(1) gather horse hair algae: gather ripe at the beginning of last month of spring in winter but not yet discharge that smart ovum, stalwartness, side shoot are many, the Leizhou sargassum of hardly staining metal or nothing as kind of an algae, male and female packing, and take Transportation.
(2) pretreatment of algae is planted: the assorted algae on kind of algae and attachment are brushed with clean writing brush in culture fluid.
(3) receptacle is isolated: in the kind algae that step (2) obtains, isolate stalwartness respectively with sterilization blade, grow one and to make peace many female, the male receptacle of side shoot, the attachment on receptacle surface is removed in culture fluid, again receptacle is separated into single or Dan Cong, is placed in scalding, cooling, the in advance warm seawater processed are deposited.
(4) Spawning is suppressed: in illumination box or culturing room, at intensity of illumination 8 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and controls culture fluid water temperature and remain on 8 DEG C, every day, a culture fluid was changed in timing, and every day timing agitation or shake culture fluid 3 times, continue 15 days.
(5) the smart ovum of discharge is promoted and fertilization is specially: mixed by female, the male receptacle 1:1 in step (4), proceed to 26 DEG C, salinity is in the culture fluid of 30, and at intensity of illumination 40 μm of olm
-2s
-1, under photoperiod 12L:12D condition, quiescent culture, every day, a culture fluid was changed in timing, and timing shake culture fluid 3 times, and each shake 0.5 minute, cultivates 12 days.
(6) nursery is specially: step (5) is cultivated the fertilized egg obtained and proceed to that temperature is 23 DEG C, salinity is in the culture fluid of 30 again, and be 120 μm of olm in intensity of illumination
-2s
-1, the photoperiod is under the condition of 12L:12D, cultivates described fertilized egg, cultivates through 18 days, obtain and be about 1.5mm, there is the seedling of 1-3 blade.
Claims (7)
1. the artificial raise seedling method of a Leizhou sargassum, it is characterized in that: by (1) collection horse hair algae, the pretreatment of (2) kind algae, (3) isolate receptacle, (4) suppress Spawning, the smart ovum of (5) promotion discharge is also fertilized and (6) nursery six steps form
Wherein said (1) gathers horse hair algae and is specially: gather ripe at the beginning of last month of spring in winter but not yet discharge that smart ovum, stalwartness, side shoot are many, the Leizhou sargassum of hardly staining metal or nothing as kind of an algae, male and female packing, and take Transportation;
Described step (4) suppresses Spawning to be specially: in illumination box or culturing room, at intensity of illumination 6-10 μm of olm
-2s
-1, photoperiod 12L:12D condition under, the receptacle that step (3) processed is placed in the culture fluid quiescent culture that salinity is 30, and control culture fluid water temperature and remain on 5-10 DEG C, every day, a culture fluid was changed in timing, and every day timing agitation or shake culture fluid 3 times, suppress Spawning to continue 15-60 days.
2. the artificial raise seedling method of a kind of Leizhou according to claim 1 sargassum, is characterized in that described (4) suppress Spawning: suppress Spawning to continue 45-60 days.
3. the artificial raise seedling method of a kind of Leizhou according to claim 1 and 2 sargassum, it is characterized in that described step (5) promotes the smart ovum of discharge and fertilization is specially: mixed by female, the male receptacle 1:1 in step (4), proceed to 23 DEG C ~ 30 DEG C, salinity is in the culture fluid of 22 ~ 34, and at intensity of illumination 40 ~ 120 μm of olm
-2s
-1, under photoperiod 12L:12D condition, quiescent culture, every day, a culture fluid was changed in timing, and timing shake culture fluid 3 times, and each shake 0.5 minute, cultivates 6-12 days.
4. the artificial raise seedling method of a kind of Leizhou according to claim 3 sargassum, it is characterized in that described (6) nursery is specially: step (5) is cultivated the fertilized egg obtained and proceed to that temperature is 23 DEG C, salinity is in the culture fluid of 30 again, and be 120 μm of olm in intensity of illumination
-2s
-1, the photoperiod is under the condition of 12L:12D, cultivates described fertilized egg, cultivates through 15-21 days, obtains the seedling with 1-3 blade.
5. the artificial raise seedling method of a kind of Leizhou according to claim 4 sargassum, is characterized in that the pretreatment of described step (2) kind algae is specially: by the attachment removing on kind of algae in culture fluid.
6. the artificial raise seedling method of a kind of Leizhou according to claim 5 sargassum, it is characterized in that described step (3) isolates receptacle: in the kind algae that step (2) obtains, isolate stalwartness respectively, grow one and to make peace many female, the male receptacle of side shoot, the attachment on receptacle surface is removed in culture fluid, again receptacle is separated into single or Dan Cong, is placed in scalding, cooling, the in advance warm seawater processed are deposited.
7. the artificial raise seedling method of a kind of Leizhou according to claim 6 sargassum, is characterized in that: described culture fluid is the seawater of via hole diameter 50 μm of strainer filterings.
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| CN104823898B (en) * | 2015-06-09 | 2017-06-06 | 广东海洋大学 | The preparation method and its feeding method of a kind of Duo Lin Xi prelarva open-mouthed baits |
| CN106069712B (en) * | 2016-07-04 | 2020-02-18 | 广东海洋大学 | Large-scale suspension summer-passing cultivation method for Sargassum leizhou seedlings based on sexual reproduction |
| CN106857226A (en) * | 2017-03-03 | 2017-06-20 | 山东省海洋生物研究院 | A kind of method of Sargassum horneri in northern productivity artificial breeding |
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| JP2006129833A (en) * | 2004-11-09 | 2006-05-25 | Japan Science & Technology Agency | Method for culturing sargassum |
| JP4765028B2 (en) * | 2006-08-29 | 2011-09-07 | 浜松ホトニクス株式会社 | Cultivation method of perennial brown algae |
| CN101422125B (en) * | 2007-10-29 | 2011-04-06 | 中国水产科学研究院黄海水产研究所 | Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method |
| CN101422127A (en) * | 2007-10-29 | 2009-05-06 | 中国水产科学研究院黄海水产研究所 | Gulf-weed sexual propagating and seedling rearing method |
| KR101309832B1 (en) * | 2011-10-27 | 2013-09-23 | 한국원자력연구원 | A method to cultivate algae using ionizing irradiation at low dose |
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