CN104221786A - Greenhouse disease-and-insect-pest controlling method using predation mites as carrier to carry bio-control bacteria - Google Patents
Greenhouse disease-and-insect-pest controlling method using predation mites as carrier to carry bio-control bacteria Download PDFInfo
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- CN104221786A CN104221786A CN201310254313.5A CN201310254313A CN104221786A CN 104221786 A CN104221786 A CN 104221786A CN 201310254313 A CN201310254313 A CN 201310254313A CN 104221786 A CN104221786 A CN 104221786A
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Abstract
The invention relates to a greenhouse disease-and-insect-pest controlling method using predation mites as a carrier to carry bio-control bacteria. The method includes four parts, namely, bio-control bacterium preparation, predation mite preparation, mixed packaging of the predation mites and the bio-control bacteria, greenhouse disease-and-insect-pest controlling using the predation mites as the carrier to carry the bio-control bacteria. In the method, the bio-control bacteria and the predation mites are required to be stored separately, pure or almost free-of-any-medium predation mites are selected, vermiculite used for packaging media must go through drying treatment, a refrigerated storage temperature is between 1-5 DEG C, when the predation mites are needed to be used as the carrier to carry the bio-control bacteria to control greenhouse diseases and insect pest, the predation mites and the bio-control bacteria are mixed, and a broadcast sowing method is adopted as a releasing method for releasing the predation mites and the bio-control bacteria. By the aid of the greenhouse disease-and-insect-pest controlling method, a potential safety hazard that the bio-control bacteria germinate in a packaging bag can be avoided, and more than 10% prevention effect can be further enhanced.
Description
Technical field
The present invention relates to a kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest, belong to agricultural pest field of biological control.
Background technology
Predatory Mites is the Important Natural Enemy of control greenhouse insect, and it carries biocontrol microorganisms and creeps and contribute to the propagation of biocontrol microorganisms, thus is conducive to the control of greenhouse disease insect pest.
Existing is that carrier carries in the method for fungi could pest controlling with Predatory Mites, containing moisture content, Predatory Mites and fungi could in Predatory Mites packaging bag, under Predatory Mites hanging bag release environment, fungi could in packaging bag may will be sprouted in the short period of time, grow mycelia, the sprouting of fungi could in packaging bag can cause fungi could preventive effect to reduce, also can affect climbing out of of Predatory Mites even cause Predatory Mites dead if simultaneously mycelial growth is full of whole packaging bag, therefore there is the hidden danger affecting preventive effect.
In order to avoid mycelia sprouts, affects preventive effect in packaging bag, need to explore new packaging and method for releasing.
Summary of the invention
The object of this invention is to provide the new method of a kind of packaging and release Predatory Mites and biocontrol microorganisms, this method can not only avoid mycelia to sprout in packaging bag, can also improve the preventive effect of more than 10%.
Comprise the following steps:
1) preparation of biocontrol microorganisms: oneself produce or buy biocontrol microorganisms, biocontrol microorganisms is pulvis, is placed on low temperature drying refrigeration between temperature 1 DEG C-5 DEG C stand-by.
2) preparation of Predatory Mites: first produce pure or almost without the Predatory Mites of any medium, then the Predatory Mites produced is divided in the Packaging Bottle containing dry vermiculite by packaging standard, is placed on deepfreeze between temperature 1 DEG C-5 DEG C after mixing stand-by.
Wherein, the pure Predatory Mites of described production refers to and first utilizes tetranychid to raise Predatory Mites on tetranychid host plant, and when waiting Predatory Mites to reach Production requirement amount, can collect Predatory Mites, the Predatory Mites collected is pure Predatory Mites; Describedly almost refer to that first utilizing prey to raise in raising medium produces Predatory Mites without the Predatory Mites of any medium, Predatory Mites output obtains conventional products when reaching conventional Packing Unit, then these products produced can be obtained almost without the Predatory Mites of any medium through reprocessing, described reprocessing refers under 0 DEG C of-35 DEG C of condition, Predatory Mites and medium thereof are poured in 20 order-100 object screen clothes and sieve, what leak down is almost without the Predatory Mites product of any medium; Described dry vermiculite refers to that vermiculite is through super-dry process, and in vermiculite, water content does not cross premature eruption in a humidity environment to avoid biocontrol microorganisms; Described Packaging Bottle refers to that Packaging Bottle body and bottle cap can seal completely, can effectively stop Predatory Mites to be escaped, bottle cap is with air-vent, air-vent size, shape are not fixed, but air-vent need be sealed by paper or gauze, gauze, cloth, and preferred air-vent is sealed by paper.
3) Predatory Mites and biocontrol microorganisms is hybrid packed: need to be that carrier carries biocontrol microorganisms when controlling greenhouse disease insect pest with Predatory Mites, take out the stand-by biocontrol microorganisms of refrigeration and bottled Predatory Mites, biocontrol microorganisms is dispensed in Predatory Mites Packaging Bottle by packaging standard, cover tightly rear mixing, be then placed between temperature 1 DEG C-5 DEG C and be transported to release areas.
4) take Predatory Mites as the method that carrier carries that biocontrol microorganisms controls greenhouse disease insect pest: after taking out the Packaging Bottle of biocontrol microorganisms and Predatory Mites, again mix the Predatory Mites in Packaging Bottle and biocontrol microorganisms gently, then the sealed paper on Packaging Bottle bottle cap or gauze, gauze are disclosed out gently, broadcast sowing on the crop leaf of damage by disease and insect generation area by the mixture of Predatory Mites and biocontrol microorganisms and vermiculite, also again can fill spray after having broadcasted sowing a biocontrol microorganisms pulvis or liquor on crop.
The present invention compares the method that existing Predatory Mites carries biocontrol microorganisms, the main distinction is, the moisture content restriction of the present invention to Predatory Mites package media requires very high, require that package media vermiculite must be drying process, Predatory Mites must be pure or almost without the product of any medium, the strict control of moisture content is conducive to intercepting biocontrol microorganisms and crosses premature eruption because meeting moisture content; The selection of refrigerated storage temperature of the present invention is between 1-5 DEG C, compares lower low temperature and is more conducive to stoping biocontrol microorganisms to be sprouted; The Predatory Mites that the present invention mentions and biocontrol microorganisms separately preserve, need to use with Predatory Mites is that carrier carries when biocontrol microorganisms controls greenhouse disease insect pest just by Predatory Mites and biocontrol microorganisms mixing, so just effectively reduce contacting of Predatory Mites and biocontrol microorganisms from the time, in case Predatory Mites body surface or Predatory Mites with few medium may contain moisture content; The present invention adopts the mode of broadcasting sowing to discharge Predatory Mites and biocontrol microorganisms, broadcast sowing release to compare hanging bag and discharge not only Predatory Mites and all discharge higher than hanging bag in short-term, even long-term preventive effect, and broadcast sowing release Predatory Mites is together discharged on greenhouse plant together with biocontrol microorganisms, the wide-scale distribution of Predatory Mites and biocontrol microorganisms can be promoted, even if biocontrol microorganisms is sprouted also high than the effect sprouting prevention and control damage by disease and insect in release bag on greenhouse plant.The present invention can not only avoid biocontrol microorganisms to sprout in packaging bag, can also improve the preventive effect of more than 10%.
Embodiment
Following methods is only the embodiment citing to claim and summary of the invention, does not represent the restriction to this claim and summary of the invention.
The preparation of embodiment 1 biocontrol microorganisms:
Oneself produce or buy biocontrol microorganisms, biocontrol microorganisms is pulvis, is placed on low temperature drying refrigeration between temperature 1 DEG C-5 DEG C stand-by.
The preparation of embodiment 2 Predatory Mites:
First produce pure or almost without the Predatory Mites of any medium, then the Predatory Mites produced is divided in the Packaging Bottle containing dry vermiculite by packaging standard, is placed on deepfreeze between temperature 1 ° C-5 DEG C after mixing stand-by.
Wherein, the pure Predatory Mites of described production refers to and first utilizes tetranychid to raise Predatory Mites on tetranychid host plant, and when waiting Predatory Mites to reach Production requirement amount, can collect Predatory Mites, the Predatory Mites collected is pure Predatory Mites; Describedly almost refer to that first utilizing prey to raise in raising medium produces Predatory Mites without the Predatory Mites of any medium, Predatory Mites output obtains conventional products when reaching conventional Packing Unit, then these products produced can be obtained almost without the Predatory Mites of any medium through reprocessing, described reprocessing refers under O DEG C of-35 DEG C of conditions, Predatory Mites and medium thereof are poured in 20 order-100 object screen clothes and sieve, what leak down is almost without the Predatory Mites product of any medium; Described dry vermiculite refers to that vermiculite is through super-dry process, and in vermiculite, water content does not cross premature eruption in a humidity environment to avoid biocontrol microorganisms; Described Packaging Bottle refers to that Packaging Bottle body and bottle cap can seal completely, can effectively stop Predatory Mites to be escaped, bottle cap is with air-vent, air-vent size, shape are not fixed, but air-vent need be sealed by paper or gauze, gauze, cloth, and preferred air-vent is sealed by paper.
Embodiment 3 Predatory Mites and biocontrol microorganisms hybrid packed:
Need to be that carrier carries biocontrol microorganisms when controlling greenhouse disease insect pest with Predatory Mites, take out the stand-by biocontrol microorganisms of refrigeration and bottled Predatory Mites, biocontrol microorganisms is dispensed in Predatory Mites Packaging Bottle by packaging standard, covers tightly rear mixing, be then placed between temperature 1 DEG C-5 DEG C and be transported to release areas.
Embodiment 4 take Predatory Mites as the method that carrier carries that biocontrol microorganisms controls greenhouse disease insect pest:
After taking out the Packaging Bottle of biocontrol microorganisms and Predatory Mites, again mix the Predatory Mites in Packaging Bottle and biocontrol microorganisms gently, then the sealed paper on Packaging Bottle bottle cap or gauze, gauze are disclosed out gently, broadcast sowing on the crop leaf of damage by disease and insect generation area by the mixture of Predatory Mites and biocontrol microorganisms and vermiculite, also again can fill spray after having broadcasted sowing a biocontrol microorganisms pulvis or liquor on crop.
Claims (5)
1. be the method that carrier carries that biocontrol microorganisms controls greenhouse disease insect pest with Predatory Mites, comprise the following steps:
1) preparation of biocontrol microorganisms: oneself produce or buy biocontrol microorganisms, biocontrol microorganisms is pulvis, is placed on low temperature drying refrigeration between temperature 1 DEG C-5 DEG C stand-by.
2) preparation of Predatory Mites: first produce pure or almost without the Predatory Mites of any medium, then the Predatory Mites produced is divided in the Packaging Bottle containing dry vermiculite by packaging standard, is placed on deepfreeze between temperature 1 DEG C-5 DEG C after mixing stand-by.
3) Predatory Mites and biocontrol microorganisms is hybrid packed: need to be that carrier carries biocontrol microorganisms when controlling greenhouse disease insect pest with Predatory Mites, take out the stand-by biocontrol microorganisms of refrigeration and bottled Predatory Mites, biocontrol microorganisms is dispensed in Predatory Mites Packaging Bottle by packaging standard, cover tightly rear mixing, be then placed between temperature 1 DEG C-5 DEG C and be transported to release areas.
4) take Predatory Mites as the method that carrier carries that biocontrol microorganisms controls greenhouse disease insect pest: after taking out the Packaging Bottle of biocontrol microorganisms and Predatory Mites, again mix the Predatory Mites in Packaging Bottle and biocontrol microorganisms gently, then the sealed paper on Packaging Bottle bottle cap or gauze, gauze are disclosed out gently, broadcast sowing on the crop leaf of damage by disease and insect generation area by the mixture of Predatory Mites and biocontrol microorganisms and vermiculite, also again can fill spray after having broadcasted sowing a biocontrol microorganisms pulvis or liquor on crop.
2. a kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest according to claim 1, it is characterized in that the pure Predatory Mites of described production refers to first utilizes tetranychid to raise Predatory Mites on tetranychid host plant, when reaching Production requirement amount Deng Predatory Mites, can collect Predatory Mites, the Predatory Mites collected is pure Predatory Mites.
3. a kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest according to claim 1, Predatory Mites almost without any medium described in it is characterized in that refers to that first utilizing prey to raise in raising medium produces Predatory Mites, Predatory Mites output obtains conventional products when reaching conventional Packing Unit, then these products produced can be obtained almost without the Predatory Mites of any medium through reprocessing, described reprocessing refers under 0 ° of C-35 DEG C of condition, Predatory Mites and medium thereof are poured in 20 order-100 object screen clothes and sieve, what leak down is almost without the Predatory Mites product of any medium.
4. a kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest according to claim 1, it is characterized in that described dry vermiculite refers to that vermiculite is through super-dry process, in vermiculite, water content did not cross premature eruption in a humidity environment to avoid biocontrol microorganisms.
5. a kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest according to claim 1, it is characterized in that described Packaging Bottle refers to that Packaging Bottle body and bottle cap can seal completely, can effectively stop Predatory Mites to be escaped, bottle cap is with air-vent, air-vent size, shape are not fixed, but air-vent need be sealed by paper or gauze, gauze, cloth, and preferred air-vent is sealed by paper.
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| CN201310254313.5A CN104221786B (en) | 2013-06-21 | 2013-06-21 | A kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104982461A (en) * | 2015-06-15 | 2015-10-21 | 中国农业科学院柑桔研究所 | Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof |
| CN105794725A (en) * | 2016-03-24 | 2016-07-27 | 首伯农(北京)生物技术有限公司 | Method for breeding predatory mites and prey mites thereof |
| CN105875517A (en) * | 2016-04-13 | 2016-08-24 | 首伯农(北京)生物技术有限公司 | Method for rapidly removing or reducing aphids in tetranychidaes or natural enemies of tetranychidaes |
| CN113115672A (en) * | 2021-04-12 | 2021-07-16 | 吉林农业科技学院 | Method for preventing and controlling underground pests based on water-soluble membrane-released mite and bacterium complex |
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| US20050178337A1 (en) * | 2004-02-17 | 2005-08-18 | Syngenta Participations Ag | System for providing beneficial insects or mites |
| CN101002572A (en) * | 2007-01-16 | 2007-07-25 | 张艳璇 | Method for controlling insects by using entomogenous fungi carried by predation-mite |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982461A (en) * | 2015-06-15 | 2015-10-21 | 中国农业科学院柑桔研究所 | Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof |
| CN104982461B (en) * | 2015-06-15 | 2018-06-01 | 中国农业科学院柑桔研究所 | Predatory Mites and the biological pesticide of Verticillium lecanii use in conjunction and its preparation, application process |
| CN105794725A (en) * | 2016-03-24 | 2016-07-27 | 首伯农(北京)生物技术有限公司 | Method for breeding predatory mites and prey mites thereof |
| CN105794725B (en) * | 2016-03-24 | 2018-11-27 | 中国农业科学院植物保护研究所 | Predatory Mites and its method for breeding of prey mite |
| CN105875517A (en) * | 2016-04-13 | 2016-08-24 | 首伯农(北京)生物技术有限公司 | Method for rapidly removing or reducing aphids in tetranychidaes or natural enemies of tetranychidaes |
| CN105875517B (en) * | 2016-04-13 | 2018-11-27 | 中国农业科学院植物保护研究所 | A kind of quick removing reduces the method for aphid or its natural enemy in tetranychid |
| CN113115672A (en) * | 2021-04-12 | 2021-07-16 | 吉林农业科技学院 | Method for preventing and controlling underground pests based on water-soluble membrane-released mite and bacterium complex |
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| CN104221786B (en) | 2016-04-27 |
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Owner name: SHOUBONONG (BEIJING) BIOTECHNOLOGY CO., LTD. Effective date: 20150828 Owner name: INST. OF PLANTS PROTECTION, CHINESE ACADEMY OF AGR Free format text: FORMER OWNER: WANG BOMING Effective date: 20150828 |
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Inventor after: Jiang Xiaohuan Inventor after: Wang Endong Inventor after: Wang Boming Inventor after: Xu Xuenong Inventor after: Lv Jiale Inventor after: Wu Shengyong Inventor before: Wang Boming |
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Free format text: CORRECT: INVENTOR; FROM: WANG BOMING TO: JIANG XIAOHUAN WANG ENDONG WANG BOMING XU XUENONG LV JIALEWU SHENGYONG |
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Effective date of registration: 20150828 Address after: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Applicant after: Institute of Plant Protection, Chinese Academy of Agricultural Sciences Applicant after: SHOUBONONG (BEIJING) BIOTECHNOLOGY CO., LTD. Address before: 100193 Beijing Old Summer Palace West Road, Haidian District, No. 2 Applicant before: Wang Boming |
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