CN102165905A - Method for controlling contagion of citrus huanglongbing by utilizing biological factors and application - Google Patents
Method for controlling contagion of citrus huanglongbing by utilizing biological factors and application Download PDFInfo
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Abstract
The invention discloses a method for controlling the contagion of citrus huanglongbing by utilizing predatory mites serving as biological factors and application. The method is characterized by comprising the following steps of: (1) clearing a garden twice every year, namely after tangerines are harvested and when tangerine spring shoots germinate, clearing the garden twice aiming at plant louse and the prevention and control of panonychus citri and other insect pests, so that the insect population of the overwintering plant louse is reduced to the minimum level by using the conventional chemical pesticide for clearing the garden and pruning insect pest twigs; and (2) releasing the predatory mites from middle-to-late March to September every year, wherein the tangerine garden is cleared comprehensively in 7 to 15 days before the predatory mites are released, the killing of the insect pests and the mites and the prevention and control of diseases are performed once aiming at the condition that the diseases, the insect pests and the mites occur in former years in the target tangerine garden, and the used medicaments are chemical pesticides which are used for killing the insect pests, the mites and bacteria effectively and are used conventionally on the market.
Description
Technical field
The present invention relates to utilize method and the purposes that biotic factor-predation mite control citrus yellow shoot disease spreads.
Background technology
Citrus yellow shoot disease Citrus huang long bing (HLB) is the cancer during global citrus is produced.Spread in the VELOCITY DIFFUSION of citrus producing region yellow twigs such as China Fujian Province, Guangdong Province, Guangxi province in recent years, cause very big economic loss with 10-20%.The most effective prevention and control measure is exactly to cut down the disease tree in large quantities all over the world at present, secondly prevents and treats unique disseminator-citrus psylla (Diaphorina citri (Kuwayama) of yellow twig with chemical agent exactly.Citrus goes out tip time disunity, send out the tip often, the tender tip time is long, the summer shoot of particularly bearing fruit does not spray medicine basically, the bud-leaf slit that the citrus psylla adult lays eggs after citrus reveals bud is located, therefore single by spray medicine control citrus psylla weak effect, the population growth of uncontrollable citrus psylla, this also is why citrus yellow shoot disease constantly spreads one of major reason that spreads.On the other hand, pest species reaches kind more than 850 in the citrus garden, but what can constitute serious harm only accounts for 1-2%, owing to rely on chemical control for a long time, destroyed the bio-diversity in citrus garden, the natural enemy mass casualties causes the big generations of insect evil mite such as citrus psylla, citrus red mite, blister mite, scale insect, leaf miner, aleyrodid, citrus fruit fly, breaks out greatly, and pesticide dosage is forced to continue to increase.Reported at present to show that the citrus psylla adult produces serious pesticide resistance to low-residual organophosphorous pesticides such as dichlorvos commonly used, Rogor, orthene, phoxim, Acetamiprids.Therefore, seeking new preventing control method and approach is a urgent task.
Summary of the invention
Purpose of the present invention just provides utilizes method and the purposes that biotic factor-predation mite control citrus yellow shoot disease spreads.The objective of the invention is to realize by following technical solution:
(1). carry out every year 2 times winter clear garden: oranges and tangerines are gathered back and tip when sprouting in oranges and tangerines spring, at wood louse is main combination control citrus red mite and other insect, carries out clear garden 2 times, and the chemical pesticide of conventional use can be used in clear garden, in conjunction with pruning sick branch, the insect population of the wood louse that survives the winter can be reduced to minimum level.
(2). in annual 3 months, all can discharge the predation mite last ten-days period-September.The quality of predation mite pest control effect, directly with indirect relation to the effect of controlling unique disseminator-citrus psylla of citrus yellow shoot disease in the orangery because: as control badly, the orchard worker still will spray insecticide, can kill the natural enemy of citrus psylla, cause wood louse out of control, break out.Therefore,, discharge predation mite specification requirement height, must realize by following technical solution in the area that citrus yellow shoot disease takes place:
A. discharge preceding 7-15 days of predation mite, oranges and tangerines are carried out once comprehensively clear garden.Mainly be situation about taking place, carry out desinsection targetedly, kill the control of mite and disease that used medicament is the conventional effective insecticidal of using in market, kill the chemical pesticide of mite, sterilization according to target orangery ground disease in former years, worm mite evil.Purpose is behind the release predation mite, not re-use chemical pesticide as far as possible.
Preceding 2-5 days of B, release predation mite, surpass 2 or other insect generation if find the quantity of the every leaf two spotted spider mite of orangery, can select biopesticide for use, as 0.26% matrine or 0.3% nimbin or Avermectin etc., prevent and treat, also can select for use the chemical pesticide of low toxicity to choose and control.
Behind C, the release predation mite, find the summer slightly or the autumn have wood louse to take place slightly, the sage is (a kind of based on kuh-seng and tobacco for available 0.26% matrine or 0.3% nimbin or Avermectin, fruit, add the 0.5% bitter cigarette aqua vegetable source pesticide that plant species is made surplus the tuber of pinellia and the monkshood etc. 10), the safe water dispersible granules of 25% Acker etc., prevent and treat, also can take out slightly, when wood louse is not also taken place, prevent with above-mentioned agricultural chemicals or low-toxin farm chemicals at oranges and tangerines.
(3), every mandarin tree burst size is one bag of predation mite more than 300, Yang Chu is carried on the back at mobile jib in the position of release, the bag top is with drawing pin or staple or other thing, the packaging bag of predation mite is fixed on a bag end leans against side shoot on the mobile jib; For rainproof, the packaging bag of predation mite is outer can to put plastic film.
(4), other insect of integrated control orangery; reducing agricultural chemicals uses; round the self regulating and control ability that helps citrus ecosystem system; structure with " with mite control mite " be core, the green prevention and control system of integrated regulation and control naturally (sward cultivation, fertilizer are used, biological medicine fertilizer use), biological control (natural enemy, botanical pesticide, plant protection product are used), physical control (light trap, fly net, yellow plate), chemical control (low-toxin farm chemicals screening).
Biotic factor-predation mite is discharged into orangery and plays two aspects:
Effect 1: by the present invention, discharge the predation mite and controlled citrus red mite, make the orchard worker buy 5-10 time chemical pesticide every year less, make unique disseminator-citrus psylla of citrus yellow shoot disease in the orangery natural enemy as: spider, ladybug, Chrysopa, minute pirate bugs etc. are subjected to the injury of agricultural chemicals less, the population of all kinds of natural enemies is multiplied normally, is lived, under the acting in conjunction of natural enemy, make that unique disseminator-citrus psylla of citrus yellow shoot disease is under control in the orangery, thereby slowed down the diffusion of citrus yellow shoot disease and the speed that spreads.
Effect 2: the experiment proved that (have video record etc. for card): predation mite, as predation mites such as amblyseius cucumeris oudeman, Si Shi genus amblyseius berlese, Pasteur's genus amblyseius berlese, can directly prey on the ovum and the nymph of citrus psylla and can finish the generation, thereby can directly control unique disseminator-citrus psylla of citrus yellow shoot disease in the orangery.
The purpose of biological and ecological methods to prevent plant disease, pests, and erosion utilizes various insects that its corresponding natural enemy is all arranged basically exactly, by protecting and utilizing natural enemy, reaches the generation of Pest Control.Biological and ecological methods to prevent plant disease, pests, and erosion can be controlled yellow twig, and to spread its principle be exactly to utilize the natural enemy of wood louse to control the breeding of wood louse, cuts off the insect vector that yellow twig is propagated, thus the spreading of control citrus yellow shoot disease.Suitably stay grass by discharging predation mite and orchard, do not use the desinsection chemical pesticide, create a living environment that helps the breeding of natural enemy population, utilize natural enemy to come the generation of Pest Control, select to use the few biopesticide of natural enemy injury is helped pest control, realize that the citrus pest control is by the transformation of chemical control to biological control.
With the experiment embodiment of the application of the amblyseius cucumeris oudeman of predation in the mite in the control citrus yellow shoot disease, further specifying the beneficial effect of this patent below, (but is not to limit the scope of the invention by any way, as yet available other predation mites control citrus yellow shoot diseases.)
Experimental example 1
Counties and cities such as the Deqing under Guangdong Province's Zhaoqing, Fengkai, height are wanted, Guangning, four meetings, it is the heavy generating region of citrus yellow shoot disease, show according to Zhaoqing bureau of agriculture survey data, at present existing 200,000 mu of citrus generation yellow twigs, account for the Zhaoqing citrus gross area 20%, and still spread every year with ten thousand mu of speed continuation of 1-2 diffusion.Zhaoqing bureau of agriculture and I cooperate the experiment of maintaining secrecy, the experiment proved that, in Gaoyao City, ground such as Deqing County, Fengkai County discharge predation in several years continuously mite--the incidence of disease of the citrus garden citrus yellow shoot disease of amblyseius cucumeris oudeman are lower than the chemoprevention garden of routine, and many healthy trees and the adjacent sick tree several years not infected phenomenons of getting along occur.
Experimental example 2
--the citrus garden of amblyseius cucumeris oudeman and do not discharge the predation mite--the conventional chemical control orchard of amblyseius cucumeris oudeman that discharges the predation mite, citrus yellow shoot disease takes place to compare with diffusion velocity:
We select 1 pair of adjacent orchard 2007-2010 in Zhaoqing Gaoyao City, tracing observation experimentizes:
Experimental example is the tight village, town, road that lives in Gaoyao City, and orchard worker Liu Yongfei and Liu Kunxiong wanted same fruit seedling field, Lian Tang town at height in 2004, bought really seedling 1000 strains of granulated sugar tangerine on the same day separately, and plant simultaneously adjacent less than 60 meters orchard in.A two fruit weather, ecology, soil, farming managerial skills unanimity.Investigation two orchard yellow twigs were respectively 15 strains, 17 strains in 2006, and the incidence of disease is respectively 1.5% and 1.7%, and all cuts down separately and wherein weigh 5 strains.2007-2010, Liu Yongfei fruit field discharges predation mite--amblyseius cucumeris oudeman in the citrus garden under the inventor's guidance, adopt " controlling mite with mite " biological control technology.And conventional chemical control is adopted at 2007-2010 in Liu Kunxiong fruit field always.As a result, these two fruit yellow twigs spread spread condition, the output situation is compared as follows table:
Table 1 " is controlled mite with mite " tangerine garden and conventional chemoprevention garden yellow twig diffusion velocity are relatively
Table 2 " is controlled mite with mite ", and tangerine garden and conventional chemoprevention garden citrus production compare
Illustrate:
A, Liu Yong fly fruit field, biological and ecological methods to prevent plant disease, pests, and erosion garden to begin at the beginning of 4 months by the end of March every year in 2007; introduce the predation mite from Inst. of Plant Protection, fujian Academy of Agricultural Science--amblyseius cucumeris oudeman release; three secondary pollutant agricultural chemicals are only sprayed in the biological and ecological methods to prevent plant disease, pests, and erosion garden of " controlling mite with mite " every year; spray is 1 time when promptly discharging before the predation mite clear garden; putting the autumn sprays 2 times after a while, and used agricultural chemicals is: 0.26% matrine or 0.3% nimbin or Avermectin.Primary pest harm such as citrus red mite, blister mite, aleyrodid, coccid do not appear in annual orchard basically, and May to September, wood louse was seldom found in the orchard basically.Investigate 10 strain orange tree in August, 2010 according to Zhaoqing bureau of agriculture, the wood louse incidence is 0%, and it is 0% that sprouting has the worm rate, and the incidence of cobweb reaches 65%, average 8.5 of every strain generating capacity.In the orchard, can find a lot of ladybugs.Natural enemy such as Chrysopa, parasitic wasp.Citrus leaves is delicate and pretty, clean free from admixture, tree vigo(u)r stalwartness.4 years newly-increased every year on average incidences of disease 0.225% of yellow twig.
The male chemoprevention fruit of b, Liu Kun field, chemical pesticides such as annual spray insecticide, miticide were 8-10 time since 2007.Used is the conventional chemical agricultural chemicals, as: go out and sweep profit, azacyclotin, Ni Suolang, Rogor, acephatemet etc.Two spotted spider mite, the annual medium harm level of blister mite.In May-September, citrus psylla is more.Investigate 10 strains in August, 2010 according to Zhaoqing bureau of agriculture, the wood louse incidence reaches 90%, and sprouting has the worm rate to reach 85%, and the cobweb incidence only has 1%, average 0.1 of strain generating capacity, and orange tree integral body looks yellow.Newly-increased citrus yellow shoot disease strain in 4 years reaches 956 strains, and it is several 95.6% to account for overall plant, the newly-increased every year on average incidence of disease 23%.At present, the orchard loses economic worth basically
Our analysis of reason that above-mentioned " controlling mite with mite " slows down the citrus yellow shoot disease rate of propagation has:
(1) direct factor: under the anatomical lens, we observe amblyseius cucumeris oudeman and can prey on the ovum and the low age nymph of citrus psylla, can finish growth, and use German Lycra anatomical lens to take the ovum and the low age nymph video overall process of amblyseius cucumeris oudeman predation citrus psylla.Amblyseius cucumeris oudeman predation citrus psylla is the direct factor that reduces communication media.
(2) indirect factor: the access times that chemical pesticide has been reduced in " controlling mite with mite " biological and ecological methods to prevent plant disease, pests, and erosion garden reach 60%-70%, improve orangery ecotope, make orangery natural enemy life condition be improved significantly, therefore, citrus psylla has been controlled by natural enemy.
Claims (6)
1. utilize method and purposes that biotic factor-predation mite control citrus yellow shoot disease spreads.It is characterized in that:
(1). carry out every year 2 times winter clear garden: oranges and tangerines are gathered back and tip when sprouting in oranges and tangerines spring, at wood louse is main combination control citrus red mite and other insect, carries out clear garden 2 times, the clear garden chemical pesticide of conventional use, in conjunction with pruning sick branch, the insect population of the wood louse that will survive the winter is reduced to minimum level;
(2). in annual 3 months, all can discharge the predation mite last ten-days period-September, discharge preceding 7-15 days of predation mite, oranges and tangerines are carried out once comprehensively clear garden, it mainly is situation about taking place according to target orangery ground disease in former years, worm mite evil, carry out desinsection targetedly, kill the control of mite and disease, used medicament is the conventional effective insecticidal of using in market, kill the chemical pesticide of mite, sterilization.
2. method according to claim 1 and purposes, it is characterized in that, described release predation mite, discharge preceding 2-5 days of predation mite, the quantity of finding the every leaf two spotted spider mite of orangery surpasses 2 or other insect generation, prevent and treat with biopesticide 0.26% matrine or 0.3% nimbin or Avermectin, or prevent and treat with the chemical pesticide of low toxicity.
3. method according to claim 1 and purposes, it is characterized in that described release predation mite is behind the release predation mite, find the summer slightly or the autumn have wood louse to take place slightly,, 25% Acker Thailand water dispersible granules holy with 0.26% matrine or 0.3% nimbin or Avermectin, fruit prevented and treated; Also can take out slightly, when wood louse is not also taken place, prevent with above-mentioned agricultural chemicals or low-toxin farm chemicals at oranges and tangerines.
4. method according to claim 1 and purposes is characterized in that, every mandarin tree burst size is one bag of predation mite more than 300, Yang Chu is carried on the back at mobile jib in the position that discharges, the bag top is fixed on the packaging bag of predation mite on the mobile jib with drawing pin or agreement nail or other thing, and the bag end leans against side shoot.
5. method according to claim 5 and purposes is characterized in that, the packaging bag of described predation mite is outer can to put plastic film.
6. according to claim 1 or 2 or 3 or 4 or 5 described method and purposes, it is characterized in that, described method and purposes comprise other insect of integrated control orangery, reducing agricultural chemicals uses, round the self regulating and control ability that helps citrus ecosystem system, structure with " with mite control mite " be core, the green prevention and control system of integrated natural regulation and control, biological control, physical control, chemical control.
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| CN102876669A (en) * | 2012-10-18 | 2013-01-16 | 广东省昆虫研究所 | Diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and identification primer and identification method thereof |
| CN103120105A (en) * | 2013-02-07 | 2013-05-29 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN103238488A (en) * | 2013-05-13 | 2013-08-14 | 福建省农业科学院植物保护研究所 | Method for comprehensively controlling dominant spider mites of potted Chinese banyans |
| CN104012280A (en) * | 2014-06-13 | 2014-09-03 | 福建农林大学 | Neem-tea tree-chamaecrista rotundifolia three-dimensional intercropping tea garden pest control method |
| CN104221786A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Greenhouse disease-and-insect-pest controlling method using predation mites as carrier to carry bio-control bacteria |
| CN104488848A (en) * | 2014-11-26 | 2015-04-08 | 曾少兰 | New method and application for controlling red spiders of Shatian pomelo by amblyseius cucumeris |
| CN104542134A (en) * | 2014-12-26 | 2015-04-29 | 广西乐业县顾式茶有限公司 | Method for preventing citrus from suffering from citrusyellowshoot by utilizing microbial organic fertilizers |
| CN104823626A (en) * | 2015-03-31 | 2015-08-12 | 杨正庭 | Agricultural ecological technique capable of preventing and controlling liberobacter asiaticum as well as harvesting organic oranges |
| CN105052659A (en) * | 2015-08-13 | 2015-11-18 | 仲恺农业工程学院 | Prevention and treatment method for citrus greening disease of fruiting production type citrus trees |
| CN105104357A (en) * | 2015-08-18 | 2015-12-02 | 首伯农(北京)生物技术有限公司 | Method of preventing and treating injurious insects and harmful mites of horticultural plants by predatory mites |
| CN105145201A (en) * | 2015-08-18 | 2015-12-16 | 中国农业科学院植物保护研究所 | Method preventing horticultural plant insects and harmful mites via natural enemies with high efficiency |
| CN105766987A (en) * | 2016-04-08 | 2016-07-20 | 广东省农业科学院植物保护研究所 | Medicament for controlling liberobacter asiaticum and preparing method thereof |
| CN105900747A (en) * | 2016-04-21 | 2016-08-31 | 厦门人天景农业科技有限公司 | Method for treating citrus yellow shoot |
| CN108718869A (en) * | 2018-05-19 | 2018-11-02 | 赣州市南康区俊萍果业发展有限公司 | A kind of Nankang's sweet tea shaddock standardized planting technology |
| CN110754275A (en) * | 2019-11-05 | 2020-02-07 | 赣南师范大学 | Method for preventing and treating citrus tree diseases and insect pests by combining biochemistry and biochemistry |
| CN113100254A (en) * | 2021-04-21 | 2021-07-13 | 福建省农业科学院植物保护研究所 | Application of amblyseius pswdolongis in prevention and treatment of psylla olivaceus and/or diaphorina citri and method thereof |
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| CN103120105A (en) * | 2013-02-07 | 2013-05-29 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN103120105B (en) * | 2013-02-07 | 2014-12-10 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN103238488A (en) * | 2013-05-13 | 2013-08-14 | 福建省农业科学院植物保护研究所 | Method for comprehensively controlling dominant spider mites of potted Chinese banyans |
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| CN104221786B (en) * | 2013-06-21 | 2016-04-27 | 中国农业科学院植物保护研究所 | A kind of method taking Predatory Mites as carrier and carry that biocontrol microorganisms controls greenhouse disease insect pest |
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| CN104488848A (en) * | 2014-11-26 | 2015-04-08 | 曾少兰 | New method and application for controlling red spiders of Shatian pomelo by amblyseius cucumeris |
| CN104542134A (en) * | 2014-12-26 | 2015-04-29 | 广西乐业县顾式茶有限公司 | Method for preventing citrus from suffering from citrusyellowshoot by utilizing microbial organic fertilizers |
| CN104823626A (en) * | 2015-03-31 | 2015-08-12 | 杨正庭 | Agricultural ecological technique capable of preventing and controlling liberobacter asiaticum as well as harvesting organic oranges |
| CN104823626B (en) * | 2015-03-31 | 2017-07-18 | 惠州市阿杨工农业科技有限公司 | A kind of prevention and control citrus yellow shoot disease harvests the Agro-ecology method of Organic Citrus simultaneously |
| CN105052659A (en) * | 2015-08-13 | 2015-11-18 | 仲恺农业工程学院 | Prevention and treatment method for citrus greening disease of fruiting production type citrus trees |
| CN105104357A (en) * | 2015-08-18 | 2015-12-02 | 首伯农(北京)生物技术有限公司 | Method of preventing and treating injurious insects and harmful mites of horticultural plants by predatory mites |
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| CN105766987B (en) * | 2016-04-08 | 2018-06-05 | 广东省农业科学院植物保护研究所 | It is a kind of to be used to prevent medicament of Citrus Huanglongbing pathogen and preparation method thereof |
| CN105900747A (en) * | 2016-04-21 | 2016-08-31 | 厦门人天景农业科技有限公司 | Method for treating citrus yellow shoot |
| CN108718869A (en) * | 2018-05-19 | 2018-11-02 | 赣州市南康区俊萍果业发展有限公司 | A kind of Nankang's sweet tea shaddock standardized planting technology |
| CN110754275A (en) * | 2019-11-05 | 2020-02-07 | 赣南师范大学 | Method for preventing and treating citrus tree diseases and insect pests by combining biochemistry and biochemistry |
| CN113100254A (en) * | 2021-04-21 | 2021-07-13 | 福建省农业科学院植物保护研究所 | Application of amblyseius pswdolongis in prevention and treatment of psylla olivaceus and/or diaphorina citri and method thereof |
| CN113100254B (en) * | 2021-04-21 | 2021-12-03 | 福建省农业科学院植物保护研究所 | Application of amblyseius pswdolongis in prevention and treatment of psylla olivaceus and/or diaphorina citri and method thereof |
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