CN102177900A - Formula and using method of novel bactericide for preventing and treating rice blast - Google Patents

Abstract

A new type of fungicide formula for preventing and treating rice blast and its application method, belonging to the compatibility of three active ingredients of tricyclazole, tebuconazole, and difenoconazole The fungicide formula is processed into a preparation to spray, soak or spread the seeds with water to prevent and control rice blast. The fungicide has the advantages of long-lasting effect, protective effect, therapeutic effect and anti-sporulation effect, which can promote the healthy growth of rice, significantly improve the seed setting rate and thousand-grain weight of rice, and control the drug resistance of pathogenic bacteria.

Description

Novel fungicide formula for controlling rice blast and its application method

1. Technical field

A new type of fungicide formula for preventing and treating rice blast and its application method, belonging to the compatible fungicide formula and its processing preparation of high-efficiency, low-toxic fungicides tricyclazole, tebuconazole, and difenoconazole three-component active ingredients method.

2. Technical background

Azole fungicides have the characteristics of wide bactericidal spectrum, high activity, fast bactericidal speed, long-lasting effect, strong systemic conductivity, etc. The main varieties are tebuconazole, difenoconazole, triadimefon, triadimefol, Hexaconazole, Propiconazole, Myclobutanil, Fenconazole, Prochloraz, etc. The mechanism of action of azole fungicides is to inhibit the biosynthesis of ergosterol, destroy the cell membrane function of pathogenic bacteria, and eventually lead to cell death, thereby playing a protective and therapeutic role. Azole fungicides can effectively control plant fungal diseases caused by Ascomycetes, Basidiomycetes and Deuteromycetes. Such fungicides have been successfully used at home and abroad to prevent and control dozens of crop diseases such as rice, cereal crops, vegetables, and fruit trees. The development and application of this type of novel bactericide provides the material basis for the present invention.

Rice blast has been an important disease that endangers rice production for a long time, and its pathogen can infect the host at any stage of rice growth, causing economic losses. At present, my country mainly uses fungicides such as tricyclazole and sulfur with moderate toxicity and only preventive effects for prevention and control. Among them, tricyclazole inhibits the synthesis of melanin in the attached spores of the blast fungus, preventing the blast fungus from invading the host and achieving specialization. The purpose of controlling rice blast. However, it takes 5-7 days for the blast fungus to invade visible disease symptoms without being visible to the naked eye, and the germ tubes where the spores germinate can also infect wounds, without the need for development and formation appressorium. Therefore, the use of tricyclazole alone to control rice blast is often ineffective because of late application time or after a storm that easily causes rice wounds. Commonly used sulfur preparations also have the problems of low activity and easy phytotoxicity in later use. Therefore, according to the occurrence and control status of rice blast, the development of new fungicides with high efficiency, broad spectrum, low toxicity and safety meets the needs of rice production.

3. Contents of the invention

Technical Problems The purpose of this invention is to research and develop a new type of fungicide formula for preventing and treating rice blast and its application method. The main technical problems it solves are that the effect of preventing and controlling rice blast is remarkable, the application period and application methods are diverse, and it can delay the development of drug resistance. Provide The fungicide formula with good compatibility of active ingredients and convenient use method.

Technical solution Select tricyclazole, tebuconazole and phenyl ether with low toxicity and low residue fungicides with high activity against rice blast fungus, long residual effect period, different action mechanism, activity in different stages of the disease cycle, and different systemic conduction properties For metroconazole, formula screening and field tests are carried out to obtain formulas showing additive or synergistic effects, and processed into preparations with different usage requirements.

Beneficial effects The present invention aims at the tricyclazole used in the existing production, which can only control rice blast but not other diseases, and has only excellent protective effect but no therapeutic effect. Triazole fungicides have broad-spectrum, high activity and excellent therapeutic effect. effect and anti-sporulation, and tebuconazole has the characteristics of rapid systemic conduction and systemic retention of difenoconazole, formula screening and field tests were carried out to obtain the formula of tricyclazole: tebuconazole: difenoconazole The compound fungicide with a weight ratio of 1:0.04-0.8:0.04-0.8 has a beneficial formula. Comparing with other pesticides, the invention has the following beneficial effects by making different preparations and carrying out indoor and field tests.

1. The present invention belongs to the formula of ternary compound fungicide, which has compatibility, expands the antibacterial spectrum, and exhibits additive and synergistic effects on different pathogenic fungi. It can not only prevent and treat rice blast, but also treat sheath blight, Rice smut and leaf smut, reducing labor and pesticide use costs.

2. The molecular targets of this formula against Magnaporthe grisea include trihydroxynaphthol reductase, tetrahydroxynaphthol reductase and 14α-demethylase, increasing the action sites, which can delay the occurrence of drug resistance and prevent and control drug-resistant diseases.

3. Tricyclazole in this formula has an excellent protective effect, tebuconazole and difenoconazole have an excellent therapeutic effect and anti-sporulation effect, and can exert activity in all stages of the disease cycle, making the fungicide The use time is more relaxed, which overcomes the problem of losing the prevention and treatment effect due to missing the best time of medication in the past.

4. The three fungicide formulas of the invention have the characteristics of being quickly absorbed by rice, can resist rainwater erosion and photolysis, and have a long residual effect period.

5. In the fungicides of the invention, tricyclazole and tebuconazole have good conductivity in the rice body, and they are transported to the upper part and leaf tip of the rice with transpiration, while difenoconazole has poor mobility and can stay in the middle and lower parts of the rice And the application site, it has a good distribution in the rice body. Therefore, it can be used by soaking seeds, spraying and spreading, and the effect of disease prevention and control is significantly improved.

6. The inventor's research proves that the triazole fungicide tebuconazole has a good physiological regulation effect on plants, which can make plants resist lodging, increase chlorophyll, reduce peroxidation, increase seed setting rate and thousand-grain weight, and increase yield.

4. Specific implementation plan and effect

(1) Formula screening

The invention uses rice seedlings as materials and rice blast fungus as objects to carry out formula screening of compound medicaments. After the original drugs of tricyclazole, tebuconazole and difenoconazole were dissolved in methanol, the ratio by weight was designed to be 1:1:1～1:0.04:0.08 (see Table 1). The aqueous solution of % Tween 20 was diluted to a series of concentrations and sprayed to treat the rice seedlings at the 4-5 leaf stage for 24 hours, and then inoculated with the spore suspension of Magnaporthe grisea (10 ⁵ spores/ml) by foliar spraying. Keep 25°C, 100% relative humidity and dark conditions within 24 hours after inoculation, and then cultivate indoors under 80% relative humidity and 12 hours photoperiod. The disease incidence was investigated 7 days after inoculation. Calculate toxicity regression equation and _EC50 value. Mixture interactions were calculated according to the Wadley method. Select a ratio with a synergistic factor (SR) value ≥ 1.5. Select six proportions for proper dosage form processing and field trials.

Table 1 Screening results of living formula

(2) Preparation of Tricyclazole Tebuconazole Difenoconazole Wettable Powder (WP) and Water Dispersible Granule (WG)

Make 40% and 44% tricyclazole tebuconazole wettable powder (WP) and 5%, 10% water respectively by E, G, I, J, L and N formula in table 1 Dispersible Granules (WG).

(3) Field use techniques and their effects

1.44% tricyclazole·tebuconazole·difenoconazole G formula (1∶0.125∶0.125) WP to prevent and control panicle blast

The experiment was arranged in Houxin Village, Huayang Town, Jurong City, Jiangsu Province. This area is an old disease area of rice blast. After monitoring, it has developed resistance to pesticides such as Yidaobangjing. The previous stubble of the test site is wheat, and the soil type of the test area is paddy and soil, with a pH value of 7.5. The test site is flat and equipped with ditches. The rice variety is Xiangfan 103. Before spraying, the leaf blast was very mild, and the disease index was about grade 1. On September 8, 2010, spray once at the early stage of rice breaching, use 44% tricyclazole·tebuconazole·difenoconazole WP 600, 750, 900g per hectare, and spray with water 750Kg/hm ² . The second time is applied at an interval of 7 days. The spraying equipment is Singapore Linong Jacto16LITROS sprayer spraying, the aperture is <0.7mm, and the medicine does not leave residual liquid.

The effect of preventing and controlling stem blast is shown in Table 1. The control effects of spraying 44% tricyclazole·tebuconazole·difenoconazole WP 600, 750, and 900g per hectare were 84.76%, 85.59%, and 89.20%, respectively. Significant difference between. The control effect of 900g application dose per hectare is the best, as high as 89.20%, which is significantly different from the control effect of the control agent. The results of the yield test showed that all treatments showed increased yield, and the application of 44% tricyclazole·tebuconazole·difenoconazole WP 900g per hectare increased the yield by 8.55% compared with the blank control.

Table 1 Field test results of 44% tripentyl benzene WP in controlling panicle blast

2. 40% tricyclazole·tebuconazole·difenoconazole E formula (1∶0.5∶0.5) WP to control panicle blast

The experiment was arranged in Jincheng Village, Guanzhuang Town, Qianshan County, Anhui Province. It is located in the mountainous area on the north bank of the Yangtze River. It is an area where rice blast frequently occurs and is seriously ill. The previous stubble of the test site is soybean, the test site is sandy loam, the fertility is medium to high, the pH value is 5.7, the field is flat, and the ditches are equipped. The rice variety is II Neiyou 111. Before spraying, the leaf blast was very mild, and the disease index was about grade 1. On August 18, 2010, spray once at the early stage of rice breaching, use 40% tricyclazole·tebuconazole·difenoconazole WP 600, 750, 900g per hectare, and spray with water 750Kg/hm ² . Apply for the second time at an interval of 12 days, and the application equipment is a "Guardian" brand manual sprayer spray.

See Table 2 for the effect of preventing and controlling stem blast. The control effects of spraying 40% tricyclazole·tebuconazole·difenoconazole WP 600, 750, and 900g per hectare are 75.50%, 76.26%, and 77.00%, respectively. The control effect of 900g application dose per hectare is the best, as high as 77.00%, which is significantly different from the control effect of the control agent. The results of the yield test showed that all treatments showed increased yield, and the application of 40% tricyclazole·tebuconazole·difenoconazole WP 900g per hectare increased the yield by 7.54% compared with the blank control.

Table 2 Field test results of 40% tripentyl benzene WP in controlling panicle blast

3. 40% tricyclazole·tebuconazole·difenoconazole I formula (1∶0.33∶0.67) WP to control panicle blast

In 2009, the experiment was arranged in An County, Sichuan Province, which is located in a mountainous area, where rice blast often occurs and is seriously ill. The previous stubble of the experimental field is soybean, the fertility of the experimental field is medium to high, the field is flat, and the ditches are equipped. The rice variety is II You 725. Before spraying, the leaf blast was very mild, and the disease index was about grade 1. Apply once at the early stage of rice breaching, use 40% tricyclazole·tebuconazole·difenoconazole WP 600, 750, 900g per hectare, and spray with water 750Kg/hm ² . The second time was applied at an interval of 7 days, and the spraying equipment was sprayed by a Singapore Linong Jacto 16LITROS manual sprayer.

See Table 3 for the effects of preventing and controlling stem blast. The control effects of spraying 40% tricyclazole·tebuconazole·difenoconazole WP 600, 750, and 900g per hectare are 65.94%, 77.93%, and 78.73%, respectively. The control effect of 900g per hectare was the best, up to 78.73%. The effects of medium and high doses of treatment were better than those of the control agent plus rice and tricyclazole. The results of the yield test showed that all treatments showed increased yield, and the application of 40% tricyclazole·tebuconazole·difenoconazole WP 900g per hectare increased the yield by 5.28% compared with the blank control.

Table 3 Field test results of 44% tripentyl benzene WP in controlling panicle blast

4.5% Tricyclazole·Tubaconazole·Difenoconazole J Formula (1∶0.33∶0.17) Granules Controlling Rice Neck Blast

The experiment was arranged in Houxin Village, Huayang Town, Jurong City, Jiangsu Province. This area is an old disease area of rice blast. After monitoring, it has developed resistance to pesticides such as Yidaobangjing. The previous stubble of the test site is wheat, and the soil type of the test area is paddy and soil, with a pH value of 7.5. The test site is flat and equipped with ditches. The rice variety is Xiangfan 103. Before spraying, the leaf blast was very mild, and the disease index was about grade 1. On August 30, 2010, it was sprayed with medicine at the rice booting stage. Use 5% tricyclazole·tebuconazole·difenoconazole granule 9000, 12000, 15000g per hectare; control drug 75% tricyclazole WP 600g. Mix and spread with 150 kg fine soil per hectare respectively. The control effect was investigated 10 days after full ear.

The effect of preventing and controlling stem blast is shown in Table 4. The results show that the formula of the present invention is processed into granules for spreading, and tricyclazole WP is mixed into "poisonous soil" for spreading, both of which have good control effects on panicle blast. Among them, the control effects of 5% tricyclazole·tebuconazole·difenoconazole granules 9000, 12000, and 15000g per hectare were 79.89%, 86.62%, and 88.33%, respectively, compared with the control agent 75% tricyclazole WP600g Dosage effects were similar.

Table 4.5% tripentyl benzene granule control panicle blast field test results

5. 10% tricyclazole·tebuconazole·difenoconazole N formula (1∶0.08∶0.04) WG seed treatment to control rice seedling blast

After soaking 100kg rice seeds with 10% tricyclazole-tebuconazole-difenoconazole WG of 200g, 250g, and 300g in water (the amount of water is 1.5 times the amount of seeds) for 48 hours (about 20°C at room temperature), Seeds are sown in pots. The spore suspension of Magnaporthe grisea was sprayed and inoculated at the 3rd to 4th leaf stage, and the disease severity of the leaves was investigated 7 to 10 days later, and the control effect was calculated.

Table 5 Field test results of 10% tripentyl benzene WG for controlling rice seedling blast

6.5% tricyclazole·tebuconazole·difenoconazole L formula (1∶0.17∶0.08) WG control rice blast seedling blast

The experiment was arranged in Louzhuang Town, Jiangyan City, Jiangsu Province. The experimental area was an old disease area, and the rice variety was Wuyujing 3. The previous stubble of the experimental field is wheat, the land is flat, the fertility is medium to high, and the irrigation facilities are complete. At the 3-leaf stage of seedlings, use 5% tricyclazole·tebuconazole·difenoconazole WG 4000, 6000, 9000 grams of preparation and 150kg of rare earth to mix and spread evenly per hectare of seedling field, and use 75% tricyclazole as the control WP 300g/hm ² is sprayed on 600kg of water. Investigate the severity of seedling leaf blast before transplanting, and calculate the control effect.

See Table 6 for the effects of preventing and controlling stem blast. The control effects of spraying 5% tricyclazole·tebuconazole·difenoconazole WP 4000, 6000, and 9000g per hectare were 80.31%, 86.22%, and 93.21%, respectively. The control effect of 900g application dose per hectare is the best, as high as 93.21%, which is significantly different from the control effect of the control agent.

Table 6 Field test results of 5% tripentylbenzene WG in controlling rice seedling blast

(4) Effect test conclusion

Indoor and field tests proved that the active ingredients of tricyclazole, tebuconazole and difenoconazole were compatible. When the total amount of tricyclazole was greater than the total amount of the other two triazole fungicides, the rice seedlings were treated after mixing different proportions of the three-component compound agent, and the control effect on the occurrence of rice blast after inoculation was the same as that of the single use There are different degrees of synergistic effect compared with the agents. The synergistic effect was the most obvious when the seedlings were treated with the ratio of tricyclazole: tebuconazole: difenoconazole 1:0.04～0.8:1:0.04～0.8. Select 6 kinds of 40% and 44% wettable powders and 5% and 10% water dispersible granules and granules processed in different proportions to prevent and control seedling blast through different methods such as water spraying, seed treatment, and spreading. And the effect of panicle blast is very ideal. Especially in the later stage, the effect of increasing production is remarkable.

Claims (3)

1. the disinfecting agent prescription and the using method thereof of control rice blast is characterized in that containing the using method of the bactericide compounded and process preparation of the ternary of tricyclazole, Tebuconazole and Difenoconazole active component.

2. the disinfecting agent prescription and the using method thereof of control rice blast according to claim 1, it is characterized in that containing sterilization or antibacterial activity composition tricyclazole: Tebuconazole: the weight ratio of Difenoconazole is 1: (0.04～0.8): the compatibility pesticidal preparations that (0.04～0.8) prescription is made.

3. the disinfecting agent prescription and the using method thereof of control rice blast according to claim 2, it is characterized in that by seed soaking or seedling stage and vegetative period method such as spray, spread fertilizer over the fields prevent and treat rice blast.