CN103733765B

CN103733765B - A kind of sterilization store method of Seeds of Dendrobium Candidum

Info

Publication number: CN103733765B
Application number: CN201410011423.3A
Authority: CN
Inventors: 朱明旺
Original assignee: YUNNAN GAOSHAN BIOLOGICAL AGRICULTURE Co Ltd
Current assignee: YUNNAN GAOSHAN BIOLOGICAL AGRICULTURE CO., LTD.
Priority date: 2014-01-09
Filing date: 2014-01-09
Publication date: 2015-11-11
Anticipated expiration: 2034-01-09
Also published as: CN103733765A

Abstract

The present invention relates to a kind of sterilization store method of Seeds of Dendrobium Candidum, belong to biological technical field.The method comprises the following steps: a, select capsule: select healthy full dendrobium candidum capsule, the excision flower base of a fruit and damage by disease and insect remain position, after cleaning solution cleaning, dry, clean after fruit surface with medicinal alcohol again, naturally dry in the shade to capsule moisture be 3% ~ 7%; B, preservation: the described capsule naturally dried in the shade is inserted bottom and is placed with in the airtight container of desiccant, with being placed in the environment of 4 DEG C ~ 6 DEG C, preserve.The invention has the beneficial effects as follows: adopt the inventive method to preserve Seeds of Dendrobium Candidum 6 months, the germination rate of seed compared with the Seeds of Dendrobium Candidum of freshly harvested without significant change.

Description

A kind of sterilization store method of Seeds of Dendrobium Candidum

Technical field

The invention belongs to biological technical field, be specifically related to a kind of sterilization store method of Seeds of Dendrobium Candidum.

Background technology

Dendrobium candidum is the orchid family Dendrobium Sw, first of before more than 1,000 years, dendrobium candidum is just classified as by medical science classics " Taoist Scriptures " " Chinese nine immortal grass ", has pressed through the celestial being grass miraculous cure that Herba Saussureae Involueratae, remote mountains glossy ganoderma etc. are widely known.Modern medicine is thought, dendrobium candidum has and improves immunity of organisms, the effect such as anti-ageing, hypoglycemic, antitumor.Dendrobium candidum because of its medical value high, artificial planting difficulty is large, gathers without limit for a long time to add that ecotope is destroyed and cause wild resource endangered.

Seeds of Dendrobium Candidum is extremely difficult in its natural state to be sprouted, and artificial culture is industry universal mode, therefore Seeds of Dendrobium Candidum reasonable, properly preserve, prevent de-vitalizing seed from having great importance.

Dendrobium candidum capsule is a undifferentiated embryo, and capsule preservation difficulty is very large.If too dry, capsule ftractures, and on the one hand, is subject to living contaminants in environment, cannot thorough disinfection sterilizing, causes seedling fostering failure; On the other hand, seed flies away in soil, cannot germinate.If capsule is too moist, easy moldy metamorphism.And Seeds of Dendrobium Candidum is along with the prolongation of resting period, and germination rate can linearly decline.So Seeds of Dendrobium Candidum is generally all sowed in one month after gathering, otherwise by inactivation, be difficult to breed.

Summary of the invention

The object of this invention is to provide a kind of sterilization store method of Seeds of Dendrobium Candidum, Seeds of Dendrobium Candidum can be deposited by the method for a long time, and has higher germination rate.

The technical solution used in the present invention is a kind of sterilization store method of Seeds of Dendrobium Candidum, and the method comprises the following steps:

A, select capsule: select healthy full dendrobium candidum capsule, the excision flower base of a fruit and damage by disease and insect remain position, after cleaning solution cleaning, dry, then after cleaning fruit surface with medicinal alcohol, naturally dry in the shade to capsule moisture be 3% ~ 7%;

B, preservation: the described capsule naturally dried in the shade is inserted bottom and is placed with in the airtight container of desiccant, with being placed in the environment of 4 DEG C ~ 6 DEG C, preserve.

Preferably, described desiccant is discolour silica gel.

Preferably, when preserving in the environment of 4 DEG C ~ 6 DEG C, each week changes primary drying agent, for controlling the temp. and humidity in airtight container well.

In the present invention, after select capsule, need the excision flower base of a fruit and damage by disease and insect to remain position, one is preserve the Conservation environment of Seeds of Dendrobium Candidum to control well, and make its temp. and humidity in sealed environment controlled, pathogeny is controlled.Hua Di is because of containing comparatively juicy, and in sealed environment, its moisture can evaporate, thus allows neutron absorption, thus seed can be made to go mouldy.

Remain after position processes at the excision flower base of a fruit and damage by disease and insect, Seeds of Dendrobium Candidum is preserved with the form of capsule, and one is in order to isolated seed contacts with external environment condition better, prevents the seed easily inactivation by external environment condition change; Two is to make seed keep active, after long-time preservation, improving the germination rate of seed.Capsule after adopting nature to dry in the shade is preserved, and the moisture of its capsule, lower than 7%, is to preserve seed better, prevents seed during preservation from naturally germinateing.Another moisture also lower than 3%, can not can affect sprouting and the germination rate of seed, moisture lower than 3% time, the germination rate of its seed can be reduced to about 65%.

In the present invention, the cleaning solution of employing is commercially available prod, for removing dust and the part germ thereof on capsule surface.Adopt discolour silica gel as desiccant, the convenient preservation condition grasped well in sealed environment, adjusts Conservation environment in time.

Beneficial effect of the present invention is: Seeds of Dendrobium Candidum of the present invention is preserved with the form of capsule, when preservation more than 6 months, the germination rate of seed compared with the Seeds of Dendrobium Candidum of freshly harvested without significant change.

Embodiment

For making those skilled in the art understand production technology of the present invention and technique effect in detail, introduce application of the present invention and technique effect further with concrete production instance below.

Embodiment one:

Select healthy full dendrobium candidum capsule, the excision flower base of a fruit and damage by disease and insect remain position, after cleaning solution cleaning, dry, then after cleaning fruit surface with medicinal alcohol, naturally dry in the shade to capsule moisture be 7%(weight ratio); The described capsule naturally dried in the shade is inserted bottom to be placed with in the airtight container of discolour silica gel, with being placed in the environment of 5 DEG C, preserves.Wherein, when preserving, a discolour silica gel is changed each week.

Embodiment two:

Select healthy full dendrobium candidum capsule, the excision flower base of a fruit and damage by disease and insect remain position, after cleaning solution cleaning, dry, then after cleaning fruit surface with medicinal alcohol, naturally dry in the shade to capsule moisture be 3%(weight ratio); The described capsule naturally dried in the shade is inserted bottom to be placed with in the airtight container of discolour silica gel, with being placed in the environment of 4 DEG C, preserves.Wherein, when preserving, a discolour silica gel is changed each week.

Embodiment three:

Select healthy full dendrobium candidum capsule, the excision flower base of a fruit and damage by disease and insect remain position, after cleaning solution cleaning, dry, then after cleaning fruit surface with medicinal alcohol, naturally dry in the shade to capsule moisture be 5%(weight ratio); The described capsule naturally dried in the shade is inserted bottom to be placed with in the airtight container of discolour silica gel, with being placed in the environment of 6 DEG C, preserves.Wherein, when preserving, a discolour silica gel is changed each week.

By to the Seeds of Dendrobium Candidum of the various embodiments described above in preservation after 6-8 month, sow, the germination rate of seed is about 95%, with the Seeds of Dendrobium Candidum of freshly harvested without significant change.

Finally it should be noted that, above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although with reference to above-described embodiment to invention has been detailed description, those skilled in the art are to be understood that, still can modify to the present invention or equivalent replacement, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in right of the present invention.

Claims

1. a sterilization store method for Seeds of Dendrobium Candidum, is characterized in that: the method comprises the following steps:

B, preservation: the described capsule naturally dried in the shade is inserted bottom and is placed with in the airtight container of desiccant, with being placed in the environment of 4 DEG C ~ 6 DEG C, preserve;

Described desiccant is discolour silica gel;

When preserving in the environment of described 4 DEG C ~ 6 DEG C, each week changes primary drying agent.