CN103858559A - Sterile forest tree seed sowing and seedling raising method - Google Patents
Sterile forest tree seed sowing and seedling raising method Download PDFInfo
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- CN103858559A CN103858559A CN201410122917.9A CN201410122917A CN103858559A CN 103858559 A CN103858559 A CN 103858559A CN 201410122917 A CN201410122917 A CN 201410122917A CN 103858559 A CN103858559 A CN 103858559A
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- seed
- forest tree
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- sterile
- husky
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000009331 sowing Methods 0.000 title claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 13
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 7
- 230000001954 sterilising effect Effects 0.000 claims abstract description 4
- 238000010899 nucleation Methods 0.000 claims description 10
- 239000004576 sand Substances 0.000 claims description 10
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 7
- 238000010790 dilution Methods 0.000 claims description 7
- 239000012895 dilution Substances 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000004563 wettable powder Substances 0.000 claims description 7
- 239000003415 peat Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000010455 vermiculite Substances 0.000 claims description 5
- 235000019354 vermiculite Nutrition 0.000 claims description 5
- 229910052902 vermiculite Inorganic materials 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 238000005253 cladding Methods 0.000 claims description 2
- 230000004345 fruit ripening Effects 0.000 claims description 2
- 239000002689 soil Substances 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 230000035784 germination Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 238000005056 compaction Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 241000931515 Acer palmatum Species 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 240000000851 Vaccinium corymbosum Species 0.000 description 1
- 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
- 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
- -1 because of germ Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021014 blueberries Nutrition 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a sterile forest tree seed sowing and seedling raising method, belonging to the field of forest cultivation technology. The sterile forest tree seed sowing and seedling raising method comprises the following steps: (1) picking ripe fruits timely in autumn and processing to obtain seeds; (2) sterilizing seeds; (3) sprouting; and (4) sowing in a sterile manner. The forest tree seeds are sowed on a sterile material before sowing, so that the emergence rate of the forest tree seeds can reach more than 90%.
Description
Technical field
The present invention relates to a kind of plant propagation technical method, specifically relate to a kind of forest tree seed aseptic seeding growing seedlings method.
Background technology
The conventional method of forest tree seed seeding and seedling raising, that seed is directly broadcasted sowing in soil, because of germ, soil insect and weeds in soil more, although adopting physics, chemical method processes soil, but still have multiple damage by disease and insect and weeds to remain in soil, the seed of sowing in soil caused harm by disease worm etc., and meets rainwater shower, cause soil compaction, cause seed to rot and can not completely normally germinate unearthed; This method be by the planting seed of vernalization on sterilizable material, the seed of germination " showing money or valuables one carries unintentionally " is emerged rapidly under aseptic condition, avoid infecting and harm of sick worm and weeds in soil, after growth of seedling a period of time, adaptability strengthens gradually, its root system can extend to continued growth in the soil in seedbed, and emergence rate is up to more than 98.0%; This method has changed traditional Mode of sowing and seedling propagation, germination rate and the emergence rate of forest tree seed are improved, solve the forest tree seed sowing reason such as worm, weeds and soil compaction due to illness in soil, all breeding bottlenecks of germination and emergence, significant in achievements in forest-tree seedling is produced and promoted.
Summary of the invention
Goal of the invention: germinate rapidly, emerge in order to ensure forest tree seed sowing and be neat, the object of the present invention is to provide a kind of simple to operate, with low cost, high forest tree seed aseptic seeding growing seedlings method of product seedling rate.
Technical scheme: the present invention is achieved through the following technical solutions:
1, a forest aseptic seeding growing seedlings method, is characterized in that comprising the steps:
(1) seed of gathering: autumn by the end of October～mid-November, in the time of seeds fruit maturation, gather in time; The fruit of gathering filters out seed after modulation, for subsequent use;
(2) seed disinfection: by 0.5～1% copper-bath seed soaking 2～4h for the seed of step (1), for subsequent use;
(3) presprouting of seeds: by the seed of step (2) and sand seed by volume: wet husky=carry out mixed sand Tibetan at 1: 2, wet husky with 800～1000 times of medicine liquid spray sterilizations of 50% carbendazol wettable powder dilution, humidity is that hand is pinched not water outlet and is advisable, the husky Tibetan initial stage is placed in the environment 60～90 days of 0～5 ℃ of temperature, be placed in temperature 25 ℃ by mixed husky seed early spring in next year, vernalization in the greenhouse of humidity 80%, radicle on the 5th～7 is broken through seed coat and is germinateed, and now can sow;
(4) aseptic seeding: by the seed of step (3), sow on sterilizable material by certain seeding row spacing, then with sterilizable material covering, with 500 times of medicine liquid sprays of 50% carbendazol wettable powder dilution.
2, the aseptic seedling-cultivating method of a kind of forest tree seed according to claim 1, is characterized in that: the seed of the described sowing of step (4) refers to the seed " showing money or valuables one carries unintentionally " after vernalization.
3, the aseptic seedling-cultivating method of a kind of forest tree seed according to claim 1, it is characterized in that: the described sterilizable material of step (4) is, by peat: perlite: vermiculite is layered on seedling bed after mixing by the volume ratio of 1: 1: 1, laying depth is 2～4cm, cladding thickness is according to kind of a grain size, be generally 1～2cm, be advisable to lose seed.
Beneficial effect: the aseptic seedling-cultivating method of forest tree seed of the present invention, the emergence rate of forest tree seed reaches more than 95.6%, and seedling-growing time is short, the neat and consistent of emerging, the nutrient that the vermiculite in sterilizable material and peat contain seedling growth, growth of seedling stalwartness; This method is easy to operation, has that cost is low, technical requirement is simple, the advantage such as easily applies, and breeds and in production, has important application and promotional value at achievements in forest-tree seedling.
Embodiment
Embodiment 1
(1) autumn by the end of October～early November, in the time that blueberry fruit is atropurpureus, gather in time; The fruit of gathering is put into water and is soaked and dry in the shade to hand-laundering out white seed after pulp deliquescing for 2～3 days, for subsequent use;
(2) 0.5% copper-bath seed soaking 4h for seed step (1) being modulated, for subsequent use;
(3) by the seed of step (2) and sand seed by volume:: wet sand=1:: 2 mix, wet husky humidity is that hand is pinched not water outlet and is advisable, first mixed husky seed is placed in to 30 ℃ of temperature, in the greenhouse of humidity 80% after 5 days, be placed in again the environment 80 days of 0～5 ℃ of temperature, after the seed of mid-March early spring in next year sand being hidden sieves, seed is put into concentration 100mg/L heteroauxin solution and soak 4h, rinse well with clear water again, be placed in 25 ℃ of temperature, vernalization in the greenhouse of humidity 80%, 5～7d radicle is broken through seed coat and is germinateed, and now can sow;
(4) seed step (3) " being showed money or valuables one carries unintentionally ", bar is sowed at and is layered in the sterilizable material that on seedling bed, thickness is 2cm, its proportioning is: peat: perlite: vermiculite=1: by volume mix at 1: 1, cover after 1cm with this sterilizable material again, with 500 times of medicine liquid sprays of 50% carbendazol wettable powder dilution, emergence rate reaches 95.6%.
Embodiment 2
(1) autumn by the end of October～early November, in the time that Acer palmatum fruit is brown, gather; After drying in the shade, the fruit of gathering removes seed wing, and for subsequent use;
(2) 1% copper-bath seed soaking 2h for seed step (1) being modulated, for subsequent use;
(3) by the seed of step (2) and sand seed by volume: wet sand=mix at 1: 2, wet husky humidity is that hand is pinched not water outlet and is advisable, and is placed on husky Tibetan 70d in the environment of 0～5 ℃ of temperature with 1000 times of medicine liquid sprays sterilizations of 50% carbendazol wettable powder dilution; After the seed of at the beginning of 3 months early springs of next year, sand being hidden sieves, seed is put into concentration 200mg/L Gibberellins solution and soak 2h, then rinse well with clear water, be placed in 25 ℃ of temperature, vernalization in the greenhouse of humidity 80%, 3～5d radicle is broken through seed coat and is germinateed, and now can sow;
(4) seed step (3) " being showed money or valuables one carries unintentionally ", program request or bar are sowed at and are layered in the sterilizable material that on seedling bed, thickness is 4cm, its proportioning is: peat: perlite: vermiculite=1: by volume mix at 1: 1, cover after 2cm with this sterilizable material again, with 500 times of medicine liquid sprays of 50% carbendazol wettable powder dilution, emergence rate reaches 98.5%.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replaces or the technical scheme that obtains of equivalent transformation mode, within all dropping on protection scope of the present invention.
Claims (3)
1. a forest aseptic seeding growing seedlings method, is characterized in that comprising the steps:
(1) seed of gathering: autumn by the end of October～mid-November, in the time of seeds fruit maturation, gather in time; The fruit of gathering filters out seed after modulation, for subsequent use;
(2) seed disinfection: by 0.5～1% copper-bath seed soaking 2～4h for the seed of step (1), for subsequent use;
(3) presprouting of seeds: by the seed of step (2) and sand seed by volume: wet husky=carry out mixed sand Tibetan at 1: 2, wet husky with 800～1000 times of medicine liquid spray sterilizations of 50% carbendazol wettable powder dilution, humidity is that hand is pinched not water outlet and is advisable, the husky Tibetan initial stage is placed in the environment 60～90 days of 0～5 ℃ of temperature, be placed in temperature 25 ℃ by mixed husky seed early spring in next year, vernalization in the greenhouse of humidity 80%, radicle on the 5th～7 is broken through seed coat and is germinateed, and now can sow;
(4) aseptic seeding: by the seed of step (3), sow on sterilizable material by certain seeding row spacing, then with sterilizable material covering, with 500 times of medicine liquid sprays of 50% carbendazol wettable powder dilution.
2. the aseptic seedling-cultivating method of a kind of forest tree seed according to claim 1, is characterized in that: the seed of the described sowing of step (4) refers to the seed " showing money or valuables one carries unintentionally " after vernalization.
3. the aseptic seedling-cultivating method of a kind of forest tree seed according to claim 1, it is characterized in that: the described sterilizable material of step (4) is, by peat: perlite: vermiculite is layered on seedling bed after mixing by the volume ratio of 1: 1: 1, laying depth is 2～4cm, cladding thickness is according to kind of a grain size, be generally 1～2cm, be advisable to lose seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410122917.9A CN103858559B (en) | 2014-03-27 | 2014-03-27 | A kind of forest tree seed aseptic seeding growing seedlings method |
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| CN201410122917.9A CN103858559B (en) | 2014-03-27 | 2014-03-27 | A kind of forest tree seed aseptic seeding growing seedlings method |
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| CN103858559A true CN103858559A (en) | 2014-06-18 |
| CN103858559B CN103858559B (en) | 2016-01-20 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519338A (en) * | 2016-01-04 | 2016-04-27 | 山东棉花研究中心 | Paeonia ostii seed seedling-raising method |
| CN113207552A (en) * | 2021-05-14 | 2021-08-06 | 山东省林业科学研究院 | Seedling mycorrhization method taking mycorrhizal fungi hypha culture medium as seedling culture medium and application of seedling mycorrhization method |
Citations (3)
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|---|---|---|---|---|
| CN101601342A (en) * | 2009-06-29 | 2009-12-16 | 张法良 | A kind of germinating method of blueberry seed |
| CN102668773A (en) * | 2012-06-05 | 2012-09-19 | 江苏省林业科学研究院 | Large-seed seedling raising method of woody plant |
| CN103477753A (en) * | 2013-10-21 | 2014-01-01 | 广西壮族自治区林业科学研究院 | Method for promoting germination of zanthoxylum dissitum hemsl seeds |
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2014
- 2014-03-27 CN CN201410122917.9A patent/CN103858559B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601342A (en) * | 2009-06-29 | 2009-12-16 | 张法良 | A kind of germinating method of blueberry seed |
| CN102668773A (en) * | 2012-06-05 | 2012-09-19 | 江苏省林业科学研究院 | Large-seed seedling raising method of woody plant |
| CN103477753A (en) * | 2013-10-21 | 2014-01-01 | 广西壮族自治区林业科学研究院 | Method for promoting germination of zanthoxylum dissitum hemsl seeds |
Non-Patent Citations (1)
| Title |
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| 仝玉琴: "花木种子的播种处理措施及播种方法", 《花草苗木》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519338A (en) * | 2016-01-04 | 2016-04-27 | 山东棉花研究中心 | Paeonia ostii seed seedling-raising method |
| CN105519338B (en) * | 2016-01-04 | 2018-05-29 | 山东棉花研究中心 | A kind of phoenix pellet seed seedling-raising method |
| CN113207552A (en) * | 2021-05-14 | 2021-08-06 | 山东省林业科学研究院 | Seedling mycorrhization method taking mycorrhizal fungi hypha culture medium as seedling culture medium and application of seedling mycorrhization method |
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| CN103858559B (en) | 2016-01-20 |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yan Yanhua Inventor after: Xing Wei Inventor after: Chou Shuguang Inventor after: Dou Quanqin Inventor before: Dou Quanqin |
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| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20151217 Address after: 211153 Jiangsu Nanjing Jiangning District East good bridge Applicant after: Jiangsu Provincial Forest Science Research Inst. Applicant after: Zhenjiang Runzhou District Forestry fruit tea guidance Station Address before: 211153 Jiangsu Nanjing Jiangning District East good bridge Applicant before: Jiangsu Provincial Forest Science Research Inst. |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Dou Quanqin Inventor after: Xing Wei Inventor after: Chou Shuguang Inventor before: Yan Yanhua Inventor before: Xing Wei Inventor before: Chou Shuguang Inventor before: Dou Quanqin |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180111 Address after: 211153 Jiangsu Nanjing Jiangning District East good bridge Patentee after: Jiangsu Provincial Forest Science Research Inst. Address before: 211153 Jiangsu Nanjing Jiangning District East good bridge Co-patentee before: Zhenjiang Runzhou District Forestry fruit tea guidance Station Patentee before: Jiangsu Provincial Forest Science Research Inst. |
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| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: Jiangsu forestry building, 22 Dinghuaimen street, Gulou District, Nanjing, Jiangsu 210036 Patentee after: JIANGSU ACADEMY OF FORESTRY Address before: 211153 Jiangsu Nanjing Jiangning District East good bridge Patentee before: JIANGSU ACADEMY OF FORESTRY |