CN104145025A - 用于检测微囊藻素生成性毒性蓝细菌的方法和引物 - Google Patents

用于检测微囊藻素生成性毒性蓝细菌的方法和引物 Download PDF

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CN104145025A
CN104145025A CN201280066592.6A CN201280066592A CN104145025A CN 104145025 A CN104145025 A CN 104145025A CN 201280066592 A CN201280066592 A CN 201280066592A CN 104145025 A CN104145025 A CN 104145025A
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seq
microcystin
sample
sequence
cyanobacteria
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H·萨维拉
M·维尼亚南
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University of Turku
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University of Turku
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/142—Toxicological screening, e.g. expression profiles which identify toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280066592.6A 2011-11-09 2012-11-09 用于检测微囊藻素生成性毒性蓝细菌的方法和引物 Pending CN104145025A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161557496P 2011-11-09 2011-11-09
US61/557,496 2011-11-09
FI20116109 2011-11-09
FI20116109 2011-11-09
PCT/FI2012/051098 WO2013068654A1 (en) 2011-11-09 2012-11-09 Method and primers for the detection of microcystin-producing toxic cyanobacteria

Publications (1)

Publication Number Publication Date
CN104145025A true CN104145025A (zh) 2014-11-12

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CN201280066592.6A Pending CN104145025A (zh) 2011-11-09 2012-11-09 用于检测微囊藻素生成性毒性蓝细菌的方法和引物

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Country Link
US (1) US20150093748A1 (enExample)
EP (1) EP2776584B1 (enExample)
JP (1) JP2014532438A (enExample)
CN (1) CN104145025A (enExample)
PL (1) PL2776584T3 (enExample)
WO (1) WO2013068654A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110741090A (zh) * 2017-05-09 2020-01-31 赛安诺生物技术有限责任公司 用于修饰微囊藻毒素和节球藻毒素的方法
WO2020232734A1 (zh) * 2019-05-21 2020-11-26 武汉藻优生物科技有限公司 使不产毒微藻产生微囊藻毒素的方法及得到的产毒微藻
CN118979117A (zh) * 2024-10-22 2024-11-19 水利部交通运输部国家能源局南京水利科学研究院 一种特异性引物探针组合及其检测试剂盒和应用

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104278081A (zh) * 2013-07-03 2015-01-14 宁波大学 一种用lamp-lfd芯片高通量检测微囊藻毒素的方法
CN103484394B (zh) * 2013-07-04 2015-02-18 河南师范大学 有毒微囊藻菌株及其毒素纯化方法
WO2018071954A1 (en) * 2016-10-17 2018-04-26 Newsouth Innovations Pty Limited Recombinant microcystin production
US12391996B2 (en) 2018-09-26 2025-08-19 The U.S. Epa Early warning genetic testing of toxic cyanobacteria in water supply
CN111518936A (zh) * 2019-09-02 2020-08-11 广州微芯生物科技有限公司 用于检测产毒铜绿微囊藻的荧光定量pcr方法及相应的试剂盒
KR102421942B1 (ko) * 2020-10-20 2022-07-19 한국수자원공사 마이크로시스티스, 독성 마이크로시스티스 검출용 프라이머 세트 및 이를 이용한 독성 마이크로시스티스 검출방법
CN113652472B (zh) * 2021-07-27 2022-07-26 壹健生物科技(苏州)有限公司 一种检测产毒微囊藻菌型的探针组合、芯片、试剂盒及方法

Citations (1)

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CN1428434A (zh) * 2002-11-14 2003-07-09 中国科学院水生生物研究所 一种检测微囊藻产毒的方法

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FI20030771A7 (fi) * 2003-05-21 2004-11-22 Helsingin Yliopisto Menetelmä toksisten syanobakteerien määrittämiseksi
EP1888766B1 (en) 2005-05-31 2013-03-27 Newsouth Innovations Pty Limited Detection of hepatotoxic cyanobacteria
WO2007005649A2 (en) * 2005-06-30 2007-01-11 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension field
WO2011003184A1 (en) 2009-07-08 2011-01-13 National Research Council Of Canada Detection of microcystin-producing cyanobacteria

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CN1428434A (zh) * 2002-11-14 2003-07-09 中国科学院水生生物研究所 一种检测微囊藻产毒的方法

Non-Patent Citations (8)

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Title
BRETT A. NEILAN,ET AL: "Nonribosomal Peptide Synthesis and Toxigenicity of Cyanobacteria", 《JOURNAL OF BACTERIOLOGY》 *
CHRISTIANSEN,G.,ET AL: "Planktothrix agardhii microcystin synthesis gene cluster", 《GENBANK AJ441056》 *
EVA SCHOBER,ET AL: "Interlaboratory comparison of Taq Nuclease Assays for the quantification of the toxic cyanobacteria Microcystis sp.", 《JOURNAL OF MICROBIOLOGICAL METHODS》 *
HENNA HAUTALA,ET AL: "Quantitative PCR detection and improved sampl epreparation of microcystin-producing Anabaena, Microcystis and Planktothrix", 《ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY》 *
NISHIZAWA,T.ET AL: "Microcystis aeruginosa mcyA, mcyB and mcyC genes, complete cds", 《GENBANK:AB019578》 *
RAINER KURMAYER , THOMAS KUTZENBERGER: "Application of Real-Time PCR for Quantification of Microcystin Genotypes in a Population of the Toxic Cyanobacterium Microcystis sp.", 《APPLIED AND ENVIROMENTAL MICROBIOGLOGY》 *
ROUHIAINEN,L.,ET AL: "Anabaena sp. 90 hypothetical protein genes, complete cds; microcystin biosynthesis gene cluster, complete sequence; and putative transposase (atn1) gene, complete cds", 《GENBANK AY212249》 *
谢数涛等: "微囊藻毒素合成酶基因的PCR检测方法", 《热带亚热带植物学报》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110741090A (zh) * 2017-05-09 2020-01-31 赛安诺生物技术有限责任公司 用于修饰微囊藻毒素和节球藻毒素的方法
WO2020232734A1 (zh) * 2019-05-21 2020-11-26 武汉藻优生物科技有限公司 使不产毒微藻产生微囊藻毒素的方法及得到的产毒微藻
CN118979117A (zh) * 2024-10-22 2024-11-19 水利部交通运输部国家能源局南京水利科学研究院 一种特异性引物探针组合及其检测试剂盒和应用

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EP2776584A1 (en) 2014-09-17
EP2776584B1 (en) 2019-01-02
PL2776584T3 (pl) 2019-05-31
WO2013068654A1 (en) 2013-05-16
JP2014532438A (ja) 2014-12-08
US20150093748A1 (en) 2015-04-02

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Application publication date: 20141112