CN104094821B - A kind of cultural method of high flavones content bitter buckwheat bud - Google Patents
A kind of cultural method of high flavones content bitter buckwheat bud Download PDFInfo
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- CN104094821B CN104094821B CN201410209129.3A CN201410209129A CN104094821B CN 104094821 B CN104094821 B CN 104094821B CN 201410209129 A CN201410209129 A CN 201410209129A CN 104094821 B CN104094821 B CN 104094821B
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- bitter buckwheat
- bud
- alcohol extract
- flavones
- sophora bud
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Abstract
The invention discloses a kind of cultural method of high flavones content bitter buckwheat bud, the method uses that first bitter buckwheat seed is put into clear water soaks, the bitter buckwheat seed after immersion is cultivated with the sophora bud alcohol extract that concentration is 5 40g/L again, to germination after the 7th day harvesting bitter buckwheat bud.The present invention cultivates bitter buckwheat bud as nutrient solution using sophora bud alcohol extract, can significantly improve the content of flavones in bitter buckwheat bud, and compared with the bitter buckwheat bud with Aquaponic, flavones content can improve 43.51%.
Description
Technical field
The present invention relates to a kind of cultural method of bitter buckwheat bud, more particularly to a kind of culture side of high flavones content bitter buckwheat bud
Method.
Background technology
Buckwheat has two kinds of bitter buckwheat and sweet buckwheat, and wherein duck wheat is the exclusive kind of China, is one of medical and edible dual purpose plant.
Duck wheat is special rich in trophic function compositions such as protein, fat, starch, dietary fiber, vitamin, mineral matter and trace elements
It is not containing the unexistent flavone compound of other cereal crops, wherein with rutin content highest, accounting for general flavone compound
85%.There are some researches show buckwheat seed is sprouted as the activity substance content that duck wheat can be significantly improved after bud.
Bitter buckwheat bud is disturbed at present, the method used with changing its ingredient mainly there are following three kinds：One is
Trace element, such as potassium nitrate, magnesium sulfate, zinc sulfate are added in nutrient solution；Two be with trace element as borax, zinc sulfate,
Manganese sulfate, copper sulphate etc. are soaked seed；Three is to change water sucting mode, absorbent time etc..Though the above method can be improved to a certain extent
The flavones content of bitter buckwheat bud, but flavones content only up to improve 20% or so (compared with the bitter buckwheat bud with Aquaponic).
The report as nutrient solution culture bitter buckwheat bud using Chinese medicine extract has not yet been viewed at present.The present invention is from rich in Huang
The alcohol extract of the bud-sophora bud of the legume Chinese scholartree (Sophora japonical) of ketone carries out bitter buckwheat bud as nutrient solution
Culture.
The content of the invention
It is an object of the invention to provide a kind of cultural method of high flavones content bitter buckwheat bud, the method uses Chinese medicine Chinese scholartree
The alcohol extract of rice carries out the culture of bitter buckwheat bud as nutrient solution, can significantly improve flavones content in bitter buckwheat bud.
To reach above-mentioned purpose, the solution that the present invention is used is：First bitter buckwheat seed is put into clear water and is soaked, then will
Bitter buckwheat seed after immersion is put into through in sterilized germination disk, then germination disk being placed in incubator and being cultivated to bitter buckwheat seed hair
The 7th day after bud, germination disk is tried to get to the heart of a matter and is placed with nutrient solution, and the nutrient solution is sophora bud alcohol extract, and its concentration is 5-40g/L.
Further, the concentration of sophora bud alcohol extract is 20g/L.
Change the sophora bud alcohol extract during culture twice sooner or later daily, the extract solution in the disk that germinates need to completely be inhaled during replacing
Go out, add fresh extract solution, if there is flavones Precipitation in extract solution, need again to be added after flavones precipitation dissolving.
The present invention as nutrient solution culture bitter buckwheat bud, can significantly improve containing for flavones in bitter buckwheat bud using sophora bud alcohol extract
Amount, compared with the bitter buckwheat bud with Aquaponic, its flavones content can improve 43.51%.
The present invention is described in further details with reference to the accompanying drawings and examples, but present disclosure is not limited in
This.
Brief description of the drawings
Fig. 1 is the comparing figure of the flavones content of the bitter buckwheat bud of sophora bud alcohol extract and aqueous extract culture.
Specific embodiment
Embodiment 1
(1) preparation of sophora bud alcohol extract
The sophora bud, plus 10 times of ethanol (edible alcohol) of 70% for measuring are weighed, 25 DEG C of ultrasound extraction 40min, filtering takes supernatant
Liquid Rotary Evaporators remove ethanol, then add 25 DEG C of hot water constant volumes, are centrifuged standby.
(2) soak seed
Selection high purity, full seed, pollution-free and placement first use clear water quick wash no more than the seed of half a year,
Place into and soak 10h in 25 DEG C of clear water, drained away the water after pulling out.
(3) culture of bitter buckwheat bud
After by germination disk sterilization, one layer of absorbent cotton first is put into its bottom, places into warp layer cloth, the kind that then will be drained
Son randomly selects 20-30 and is put into germination disk, and adds sophora bud alcohol extract, and the addition of sophora bud alcohol extract is not planted with
The amount of the 1/3 of sub- height is advisable.Cultivated during germination disk is placed in into 25 DEG C, the incubator of relative humidity 80%, sooner or later twice daily
Nutrient solution is changed, the mother liquor in the disk that germinates need to completely be suctioned out when changing nutrient solution every time, add the nutrient solution of Fresh,
If there is flavones Precipitation in nutrient solution, need to be put into nutrient solution in ultrasonic instrument carry out it is ultrasonically treated, after its dissolving after again
Addition, the 7th day harvesting bitter buckwheat bud after bitter buckwheat germination.
(4) result and analysis
The related data such as following table of the bitter buckwheat bud that sophora bud alcohol extract is cultivated：
| Sophora bud alcohol extract concentration (g/L) | 40 | 30 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.168 | 0.217 | 0.226 | 0.18 | 0.24 | 0.266 |
| Flavones content percentage (%) | 1.707 | 1.657 | 2.266 | 1.787 | 1.607 | 1.579 |
Note：Sophora bud alcohol extract concentration is 0 to refer to clear water in table.
Be can be seen that from Fig. 1 and table, in scope of experiment, contained using flavones in the bitter buckwheat bud of sophora bud alcohol extract culture
Amount is above the content of flavones in the bitter buckwheat bud using clear water culture, and when the concentration of sophora bud alcohol extract is 20g/L, flavones contains
The rising of amount is most obvious, and the content than flavones in the bitter buckwheat bud with clear water culture is higher by 43.51%, it can be seen that, sophora bud alcohol extracting
The optium concentration for taking liquid cultivation bitter buckwheat bud is 20g/L.
Comparing embodiment 1
The nutrient solution of bitter buckwheat bud in embodiment 1 is changed to sophora bud aqueous extract by sophora bud alcohol extract, other and embodiment 1
It is identical；
The sophora bud aqueous extract is adopted and prepared with the following method：The sophora bud is weighed, after appropriate crushing, plus 10 times of water immersions of amount
30min, is then heated with low baking temperature and boils 30min, is filtered, and the dregs of a decoction add the water of same amount again, is heated to boiling 30min, and filtering will twice
Filtrate merges, and is centrifuged standby；
The related data such as following table of the bitter buckwheat bud cultivated：
| Sophora bud aqueous extract concentration (g/L) | 4030 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.1280.217 | 0.275 | 0.178 | 0.199 | 0.266 |
| Flavones content percentage (%) | 1.5391.589 | 1.643 | 1.621 | 1.605 | 1.579 |
Note：Sophora bud aqueous extract concentration is 0 to refer to clear water in table.
Be can be seen that from Fig. 1 and table, in scope of experiment, bitter buckwheat can also be improved using sophora bud aqueous extract culture bitter buckwheat bud
The content (concentration is for except 40g/L) of flavones in bud, but significantly lower than flavones in the bitter buckwheat bud using sophora bud alcohol extract culture
Content.
Comparing embodiment 2
The nutrient solution of bitter buckwheat bud in embodiment 1 is changed to root of large-flowered skullcap alcohol extract or the extraction of root of large-flowered skullcap water by sophora bud alcohol extract
Liquid, other are same as Example 1, the preparation method of the root of large-flowered skullcap alcohol extract and root of large-flowered skullcap aqueous extract respectively with embodiment 1 and
Comparing embodiment 1 is identical, and simply raw material is changed to the root of large-flowered skullcap, and the related data of the bitter buckwheat bud cultivated is respectively such as following table：
| Root of large-flowered skullcap alcohol extract concentration (g/L) | 40 | 30 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.122 | 0.213 | 0.216 | 0.218 | 0.19 | 0.266 |
| Flavones content percentage (%) | 1.427 | 1.08 | 1.368 | 1.388 | 1.255 | 1.579 |
| Root of large-flowered skullcap aqueous extract concentration (g/L) | 40 | 30 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.224 | 0.195 | 0.23 | 0.205 | 0.237 | 0.266 |
| Flavones content percentage (%) | 1.632 | 1.65 | 1.39 | 1.411 | 1.422 | 1.579 |
Note：Extract concentration is 0 to refer to clear water in table.
Be can be seen that from upper table, in scope of experiment, flavones contains during the root of large-flowered skullcap alcohol extract of various concentrations makes bitter buckwheat bud
Amount is reduced, and simply reduces degree difference, and as a whole, when root of large-flowered skullcap alcohol extract concentration is 30g/L, flavones content reduction is most strict
Evil.Flavones content is presented the low concentration reduction elevated phenomenon of high concentration in the bitter buckwheat bud that root of large-flowered skullcap aqueous extract is cultivated, when concentration is
5th, 10, reduction effect is basically identical during 20g/L, the content of flavones all 1.4% or so, but when the concentration of root of large-flowered skullcap aqueous extract is
During 30g/L, the content of flavones is raised, this effect contrast with root of large-flowered skullcap alcohol extract.
Comparing embodiment 3
The nutrient solution of bitter buckwheat bud in embodiment 1 is changed to rhodiola root alcohol extract or rhodiola root water extraction by sophora bud alcohol extract
Take liquid, other are same as Example 1, the preparation method of the rhodiola root alcohol extract and rhodiola root aqueous extract respectively with implementation
Example 1 and comparing embodiment 1 are identical, and simply raw material is changed to rhodiola root, and the related data of the bitter buckwheat bud cultivated is respectively such as following table：
| Rhodiola root alcohol extract concentration (g/L) | 40 | 30 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.231 | 0.206 | 0.233 | 0.237 | 0.187 | 0.266 |
| Flavones content percentage (%) | 1.493 | 1.365 | 1.479 | 1.444 | 1.675 | 1.579 |
| Rhodiola root aqueous extract concentration (g/L) | 40 | 30 | 20 | 10 | 5 | 0 |
| Shoot dry matter (g) | 0.275 | 0.205 | 0.212 | 0.195 | 0.246 | 0.266 |
| Flavones content percentage (%) | 1.287 | 1.482 | 1.467 | 1.564 | 1.639 | 1.579 |
Note：Extract concentration is 0 to refer to clear water in table.
Be can be seen that from upper table, in scope of experiment, except concentration be the rhodiola root alcohol extracting of 5g/L, aqueous extract can make hardship
Outside the percentage composition of flavones is raised in buckwheat bud, the extract solution of remaining each concentration makes flavones content reduction in bitter buckwheat bud, wherein
When rhodiola root alcohol extract concentration is 30g/L and aqueous extract concentration is 40g/L, the content of flavones is minimum in bitter buckwheat bud.
Be can be seen that from comparing embodiment 2 and comparing embodiment 3, although the sophora bud, the root of large-flowered skullcap and rhodiola root are all to contain flavones
Chinese medicine, but their alcohol, aqueous extract is for being not the content that can improve flavones in bitter buckwheat bud when cultivating bitter buckwheat bud.
Claims (3)
1., first be put into bitter buckwheat seed in clear water and soak by a kind of cultural method of high flavones content bitter buckwheat bud, then by after immersion
Bitter buckwheat seed is put into through in sterilized germination disk, being cultivated during germination disk then is placed in into 25 DEG C, the incubator of relative humidity 80%
The 7th day after to bitter buckwheat germination, the germination disk is tried to get to the heart of a matter and is placed with nutrient solution, it is characterised in that：The nutrient solution is sophora bud alcohol
Extract solution, its concentration is 5-40g/L, and the amount that sophora bud alcohol extract presses do not had seed height 1/3 is added, sophora bud alcohol extract
Preparation method is：The sophora bud, plus 10 times of ethanol of 70% for measuring are taken, 25 DEG C of ultrasound extraction 40min, filtering takes supernatant rotation
Evaporimeter removes ethanol, then adds 25 DEG C of hot water constant volumes, centrifugation to be obtained.
2. the cultural method of high flavones content bitter buckwheat bud according to claim 1, it is characterised in that：Sophora bud alcohol extract
Concentration is 20g/L.
3. the cultural method of high flavones content bitter buckwheat bud according to claim 1 and 2, it is characterised in that：Needed during culture every
It changes sophora bud alcohol extract twice sooner or later, need to completely be suctioned out the extract solution in the disk that germinates during replacing, adds fresh carrying
Liquid is taken, if there is flavones Precipitation in extract solution, needs again to be added after flavones precipitation dissolving.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2729743C1 (en) * | 2020-02-27 | 2020-08-11 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр агробиотехнологий Дальнего Востока им. А.К. Чайки" (ФГБНУ "ФНЦ агробиотехнологий Дальнего Востока им. А.К. Чайки") | Method for increasing content of flavonoids in buckwheat fruits |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941732A (en) * | 2016-04-07 | 2016-09-21 | 北京农学院 | Tartarian buckwheat sprout tea and manufacturing method thereof |
| CN110082248B (en) * | 2019-05-06 | 2020-03-31 | 东北林业大学 | Method for determining use concentration of leaching liquor in biological assay of seed germination inhibitor and application of method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273022A (en) * | 1999-05-05 | 2000-11-15 | 杨天寿 | Method for cultivating multi-vitamine nutritive vegetables |
| CN101695248A (en) * | 2009-10-30 | 2010-04-21 | 何飞翔 | Method for cultivating kudzuvine root |
| CN102523862A (en) * | 2011-11-16 | 2012-07-04 | 沈阳双鼎制药有限公司 | Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection |
| CN102893783A (en) * | 2012-10-08 | 2013-01-30 | 四川师范大学投资管理有限公司 | Method for culturing Fagopyrum tataricum(L.)Gaertn, Fagopyrum tataricum(L.)Gaertn powder and preparation thereof |
| CN103563624A (en) * | 2013-10-17 | 2014-02-12 | 杨茂忠 | Green vegetable planting method |
| CN103563615A (en) * | 2013-09-28 | 2014-02-12 | 枞阳县新少圩种粮农民专业合作社 | Culturing method for duckwheat |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006020565A (en) * | 2004-07-08 | 2006-01-26 | Mori Sangyo Kk | Method and device for producing sprout vegetable highly containing polyphenol |
| KR101206707B1 (en) * | 2010-04-15 | 2012-11-29 | 한국식품연구원 | Cultivation method of plant with increased contents of functional materials |
-
2014
- 2014-05-18 CN CN201410209129.3A patent/CN104094821B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1273022A (en) * | 1999-05-05 | 2000-11-15 | 杨天寿 | Method for cultivating multi-vitamine nutritive vegetables |
| CN101695248A (en) * | 2009-10-30 | 2010-04-21 | 何飞翔 | Method for cultivating kudzuvine root |
| CN102523862A (en) * | 2011-11-16 | 2012-07-04 | 沈阳双鼎制药有限公司 | Cultivation and quality control method for ixeris sonchifolia hance for Kudiezi injection |
| CN102893783A (en) * | 2012-10-08 | 2013-01-30 | 四川师范大学投资管理有限公司 | Method for culturing Fagopyrum tataricum(L.)Gaertn, Fagopyrum tataricum(L.)Gaertn powder and preparation thereof |
| CN103563615A (en) * | 2013-09-28 | 2014-02-12 | 枞阳县新少圩种粮农民专业合作社 | Culturing method for duckwheat |
| CN103563624A (en) * | 2013-10-17 | 2014-02-12 | 杨茂忠 | Green vegetable planting method |
Non-Patent Citations (2)
| Title |
|---|
| 芦荟粗提物对苹果采后灰霉病的防治效果与机理;袁仲玉 等;《农业工程学报》;20140228;第30卷(第4期);第255-261页 * |
| 芽苗菜的生产特点及家庭栽培方法;刘金霞 等;《新疆农业科技》;20040531(第3期);第30页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2729743C1 (en) * | 2020-02-27 | 2020-08-11 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр агробиотехнологий Дальнего Востока им. А.К. Чайки" (ФГБНУ "ФНЦ агробиотехнологий Дальнего Востока им. А.К. Чайки") | Method for increasing content of flavonoids in buckwheat fruits |
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