RU2729743C1 - Method for increasing content of flavonoids in buckwheat fruits - Google Patents

Abstract

FIELD: agriculture.SUBSTANCE: invention relates to the agriculture. Method for increasing content of flavonoids in buckwheat fruits includes sowing, treatment of plants, harvesting, wherein treatment of plants in crops is carried out in a phase of budding with water-alcohol extract in concentration of 100 mcg/ml, obtained from buckwheat plants with red staining and antioxidant activity of 99.3–99.4 %.EFFECT: invention allows to increase content of flavonoids in buckwheat fruits due to use of cheap available vegetal raw material with simultaneous reduction of the preparation consumption.1 cl, 4 tbl

Description

The invention relates to agriculture, in particular to herbal products for treating plants as growth stimulants and is intended for use in plant growing.

A known method for regulating the content of flavonols in buckwheat, including growing plants, treating them with a physiologically active gibberellin preparation containing gibersib at a concentration of 100-200 mg / l and a growth regulator of 2.4 D at a concentration of 0.1; 0.5; 5 g / l, in the phase of four true leaves and budding, and the determination of flavonols is carried out in the reproductive organs and leaves. (see, for example, Buckwheat as a source of flavonoids / V.E. Kiselev, V.I. Kovalenko, V.G. Minaeva, A.V. Kiselyova, T.A. Volkhonskaya, T.A. Zhanaeva, A.V. Laptev, - Novosibirsk: Nauka, 1985, p. 61-68).

The disadvantage of this method is the low efficiency of the drugs used, which reduce the content of flavonoids in plants.

A known method for regulating the content of flavonols in buckwheat, including growing plants, processing them with an endogenous phenolic substance isolated from the aerial part of buckwheat plants and consisting of a mixture of phenol carboxylic acids, rutin, quercitrin, quercetin at a concentration of 100 mg / l, in the phase of four true leaves and budding with an interval of 10 days, and the determination of flavonols is carried out in the reproductive organs and leaves. (see, for example, Buckwheat as a source of flavonoids / V.E. Kiselev, V.I. Kovalenko, V.G. Minaeva, A.V. Kiselyova, T.A. Volkhonskaya, T.A. Zhanaeva, A.V. Laptev, - Novosibirsk: Nauka, 1985, pp. 58-61).

The disadvantage of this method is the high complexity of obtaining drugs and repeated processing.

A known method of increasing the content of rutin in buckwheat seeds, including soaking the seeds in a solution of lurastim (n.o. 1.15 × 10 ^-6 and 10 ^-7 g / l, sowing, in the budding phase, spraying plants with a solution of modus (n.o. 4 , 4 × 10 ^-3 mol / L (see, for example, Influence of growth regulators lurastim and moddus on the content of rutin in buckwheat seeds / A.V. Korotkov, L.D. Prusakova, S.L. Belopukhov, A.N. Fesenko, V.V. Vakulenko // Agrochemistry, 2010. No. 12. P. 18-23).

The disadvantage of this method is the use of expensive drugs and their low stimulating efficiency.

A known method of increasing the content of rutin in buckwheat, including pre-sowing treatment of seeds with a solution of quartazine at a dose of 0.16-10.0 g / t and 24-epibrassinalid at a dose of 1.0-10.0 mg / t. (see, for example, patent BY, No. 5828, class IPC A01N 33/26, 45/00, 30.12.2003).

The disadvantage of this method is that the treatment of seeds with solutions of quartazine and 24-epibrassinalide is carried out in 1% starch gel, which complicates the technical solution of the problem, while the preparations are synthetic, obtained as a result of the synthesis of chemicals.

The purpose of the invention is to increase the content of flavonoids in buckwheat fruits, due to the use of cheap available plant raw materials while reducing the consumption of the drug.

This goal is achieved by the fact that in the method for increasing the content of flavonoids in buckwheat fruits, including sowing, processing plants, harvesting, according to the invention, the treatment of plants in crops is carried out in the budding phase with a water-alcohol extract at a concentration of 100 μg / ml obtained from buckwheat plants with red color of stems and antioxidant activity 99.3-99.4%.

Compared with the prototype, the features of the inventive step of the proposed method for increasing the content of flavonoids in buckwheat fruits are as follows:

1. "Treatment of plants in crops is carried out in the budding phase with a water-alcohol extract at a concentration of 100 μg / ml ...", which allows:

- use environmentally friendly means - herbal preparations;

- improve the quality of buckwheat fruits as a source of P-vitamins and improve the dietary properties of the fruits, which is important for the prevention of diseases associated with disorders of blood vessels.

2. "... obtained from buckwheat plants with red color of stems and antioxidant activity of 99.3-99.4%", which allows:

- to expand the range of products of natural origin with high antioxidant activity;

- to reduce the concentration of the solution for processing plants and the amount of the drug used and thus reduce its labor intensity, the cost of drugs;

- increase the content of flavonoids in buckwheat fruits and get food with antioxidant properties.

The features indicated in the distinctive part of the description of achieving the goal prove that the claimed method has novelty. The totality of the features, given in comparison of the properties of the claimed and known solutions, gives grounds to conclude that the claimed method of increasing the content of flavonoids in buckwheat fruits has an inventive step.

The proposed method for increasing the content of flavonoids in buckwheat fruits is as follows. Seeds of buckwheat (Fagopyrum esculentum Moench) varieties Izumrud and varieties At 7 are sown into the prepared soil. The size of the plot plot for each variety is 10 square meters, in triplicate. Agricultural technology in the experience is generally accepted for the cultivation of buckwheat in the Primorsky Territory. For the treatment of plants in crops, a water-alcohol extract is prepared. In the phase of mass flowering of buckwheat, its aerial part with different color of the stems (red, red-green, green-red, green) is collected. Selected samples are dried by air-shadow method. Grind in a mill for grinding dry samples to a fraction of 1 mm. Extracted with ethyl alcohol (C ₂ H ₅ OH - 70%) in a boiling water bath for 60 minutes. A dry extract is obtained. The antioxidant activity of the extract is determined by the spectrophotometric method (according to Bloisome) on a Multiskan FC flatbed spectrophotometer (Termo Scientific, Canada) at a wavelength of 517 nm and concentrations of 1; ten; 100 μg / ml. Studies have shown that extracts from the aboveground part of buckwheat, collected in the phase of mass flowering, have a pronounced antioxidant activity. The data obtained are presented in table 1 (grade Izumrud) and table 2 (grade Pri 7).

Table 1 shows that an aqueous-alcoholic extract with a concentration of 100 μg / ml from buckwheat plants with red stems of the Izumrud variety has the highest antioxidant activity equal to 99.3 ± 0.2%.

Table 2 shows that an aqueous-alcoholic extract with a concentration of 100 μg / ml from buckwheat plants with a red coloration of stems of cultivar Pry 7 has the highest antioxidant activity equal to 99.4 ± 0.2%.

For processing buckwheat plants in crops, an extract with a concentration of 1; ten; 100 μg / ml at the onset of the plant budding phase. Plants without extract treatment are used as a control option. At the onset of the maturation phase, 75-80% of the plants start harvesting. After harvesting and bringing the crop up to standard standards, the buckwheat fruit is analyzed for flavonoid content. The content of flavonoids is determined using a Shimadzu UV mini-1240 spectrophotometer (Japan). The results are presented in table 3 (grade Emerald) and table 4 (grade At 7).

The data in Table 3 shows that the maximum content of flavonoids in buckwheat fruits was obtained in the variant when treating crops with VSE from red plant stems at a concentration of 100 μg / ml and is 0.22 ± 0.02%.

The data in Table 4 shows that the maximum content of flavonoids in buckwheat fruits was obtained in the variant when treating crops with VSE from red plant stems at a concentration of 100 μg / ml and is 0.22 ± 0.02%.

The use of the proposed method will increase the content of flavonoids in buckwheat fruits due to the use of affordable, cheap natural and environmentally friendly raw materials with high antioxidant activity.

Claims (1)

A method of increasing the content of flavonoids in buckwheat fruits, including sowing, processing plants, harvesting, characterized in that the processing of plants in crops is carried out in the budding phase with a water-alcohol extract at a concentration of 100 μg / ml obtained from buckwheat plants with red stems and antioxidant activity 99.3-99.4%.